Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates
Accurate assays for the detection of antibodies to SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) are essential for the control of the COVID-19 (coronavirus disease 2019) pandemic. Here, we report antibody and antibody-avidity assays, relying on near-infrared-fluorescence amplification...
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| Veröffentlicht in: | Nature biomedical engineering 2020-12, Vol.4 (12), p.1188-1196 |
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| creator | Liu, Tiancheng Hsiung, Jessica Zhao, Su Kost, Jessica Sreedhar, Deepika Hanson, Carl V. Olson, Kjerstie Keare, Douglas Chang, Shin Ting Bliden, Kevin P. Gurbel, Paul A. Tantry, Udaya S. Roche, John Press, Cynthia Boggs, John Rodriguez-Soto, Jorge P. Montoya, Jose G. Tang, Meijie Dai, Hongjie |
| description | Accurate assays for the detection of antibodies to SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) are essential for the control of the COVID-19 (coronavirus disease 2019) pandemic. Here, we report antibody and antibody-avidity assays, relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates, for the simultaneous detection of antibodies to the S1 subunit of the spike protein and to the receptor binding domain of SARS-CoV-2 in human serum and saliva, and for quantifying immunoglobulin avidities against coronavirus antigens from SARS-CoV-2, SARS-CoV-1 and the common-cold viruses OC43, HKU1, NL63 and 229E. The antibody assay detected immunoglobulin M in 87% (52 of 60) COVID-19-positive serum samples collected 6 or more days after symptom onset (and the immunoglobulins M and G in all 33 samples collected at least 15 days after symptom onset), and correctly classified 456 out of the 457 COVID-19-negative serum samples tested (424 of them collected before the pandemic, including 73 that were positive for other viruses). We used the antibody-avidity assay to study antibody-maturation patterns, anamnestic responses, and cross-immunity to the common-cold coronaviruses.
Antibody and antibody-avidity assays relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates accurately quantify antibodies to SARS-CoV-2 and to common viruses in human serum and saliva. |
| doi_str_mv | 10.1038/s41551-020-00642-4 |
| format | Article |
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Here, we report antibody and antibody-avidity assays, relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates, for the simultaneous detection of antibodies to the S1 subunit of the spike protein and to the receptor binding domain of SARS-CoV-2 in human serum and saliva, and for quantifying immunoglobulin avidities against coronavirus antigens from SARS-CoV-2, SARS-CoV-1 and the common-cold viruses OC43, HKU1, NL63 and 229E. The antibody assay detected immunoglobulin M in 87% (52 of 60) COVID-19-positive serum samples collected 6 or more days after symptom onset (and the immunoglobulins M and G in all 33 samples collected at least 15 days after symptom onset), and correctly classified 456 out of the 457 COVID-19-negative serum samples tested (424 of them collected before the pandemic, including 73 that were positive for other viruses). We used the antibody-avidity assay to study antibody-maturation patterns, anamnestic responses, and cross-immunity to the common-cold coronaviruses.
Antibody and antibody-avidity assays relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates accurately quantify antibodies to SARS-CoV-2 and to common viruses in human serum and saliva.</description><subject>38/79</subject><subject>42/34</subject><subject>631/61/32</subject><subject>692/699/255/2514</subject><subject>82/1</subject><subject>96/47</subject><subject>Aged</subject><subject>Amplification</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Affinity - immunology</subject><subject>Antigens</subject><subject>Antigens, Viral - immunology</subject><subject>Assaying</subject><subject>Avidity</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Coronaviridae</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>COVID-19 - blood</subject><subject>COVID-19 - immunology</subject><subject>COVID-19 Serological Testing - methods</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Gold</subject><subject>Humans</subject><subject>I.R. radiation</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulin M - blood</subject><subject>Immunoglobulin M - immunology</subject><subject>Immunoglobulins</subject><subject>Nanostructure</subject><subject>Pandemics</subject><subject>Pandemics - prevention & control</subject><subject>Plasmonics</subject><subject>Saliva</subject><subject>Saliva - immunology</subject><subject>SARS-CoV-2 - immunology</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Spike protein</subject><subject>Substrates</subject><subject>Viral diseases</subject><subject>Viruses</subject><issn>2157-846X</issn><issn>2157-846X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtLHTEYhkNpUbH-ARcy4KabtLmfzFIO9gJCqYq4C7lNicxMTvPNCO7605vxeKy4cJXb875f4EHomJLPlHD9BQSVkmLCCCZECYbFO3TAqFxhLdTt-xf7fXQEcEcIoS0X7UruoX3OKWNa8gP099dsxyl1ydsp5bHJXbOcXQ4Pjb1PIU0pQr0KjfV-LnaKTYhT9Dv46uzyCq_zDWa73MKnsYFY5uExCLZP97ap_Ka3MOQx-QZmB9PSBh_Rh872EI-e1kN0_fX8ev0dX_z89mN9doG9oO2EI7HRWydVq5WQqzZKGnXQimktWBCdD51SznLfqZY471SkLlAlrRTOOcIP0adt7abkP3OEyQwJfOx7O8Y8g2FCKkFapkVFT1-hd3kuY_1cpVacVUjSSrEt5UsGKLEzm5IGWx4MJWYxZLaGTDVkHg2ZpfrkqXp2QwzPkZ2PCvAtAPVp_B3L_9lv1P4DQW6dIw</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Liu, Tiancheng</creator><creator>Hsiung, Jessica</creator><creator>Zhao, Su</creator><creator>Kost, Jessica</creator><creator>Sreedhar, Deepika</creator><creator>Hanson, Carl V.</creator><creator>Olson, Kjerstie</creator><creator>Keare, Douglas</creator><creator>Chang, Shin Ting</creator><creator>Bliden, Kevin P.</creator><creator>Gurbel, Paul A.</creator><creator>Tantry, Udaya S.</creator><creator>Roche, John</creator><creator>Press, Cynthia</creator><creator>Boggs, John</creator><creator>Rodriguez-Soto, Jorge P.</creator><creator>Montoya, Jose G.</creator><creator>Tang, Meijie</creator><creator>Dai, Hongjie</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3378-8071</orcidid><orcidid>https://orcid.org/0000-0002-6707-1597</orcidid><orcidid>https://orcid.org/0000-0002-8541-5752</orcidid><orcidid>https://orcid.org/0000-0003-3432-4479</orcidid><orcidid>https://orcid.org/0000-0002-4906-4502</orcidid></search><sort><creationdate>20201201</creationdate><title>Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates</title><author>Liu, Tiancheng ; Hsiung, Jessica ; Zhao, Su ; Kost, Jessica ; Sreedhar, Deepika ; Hanson, Carl V. ; Olson, Kjerstie ; Keare, Douglas ; Chang, Shin Ting ; Bliden, Kevin P. ; Gurbel, Paul A. ; Tantry, Udaya S. ; Roche, John ; Press, Cynthia ; Boggs, John ; Rodriguez-Soto, Jorge P. ; Montoya, Jose G. ; Tang, Meijie ; Dai, Hongjie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-e0aecab569864579e51e8d8628842d4fcdf66ba3cf690bcb6e1bd165a54bbb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>38/79</topic><topic>42/34</topic><topic>631/61/32</topic><topic>692/699/255/2514</topic><topic>82/1</topic><topic>96/47</topic><topic>Aged</topic><topic>Amplification</topic><topic>Antibodies</topic><topic>Antibodies, Viral - 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Academic</collection><jtitle>Nature biomedical engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Tiancheng</au><au>Hsiung, Jessica</au><au>Zhao, Su</au><au>Kost, Jessica</au><au>Sreedhar, Deepika</au><au>Hanson, Carl V.</au><au>Olson, Kjerstie</au><au>Keare, Douglas</au><au>Chang, Shin Ting</au><au>Bliden, Kevin P.</au><au>Gurbel, Paul A.</au><au>Tantry, Udaya S.</au><au>Roche, John</au><au>Press, Cynthia</au><au>Boggs, John</au><au>Rodriguez-Soto, Jorge P.</au><au>Montoya, Jose G.</au><au>Tang, Meijie</au><au>Dai, Hongjie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates</atitle><jtitle>Nature biomedical engineering</jtitle><stitle>Nat Biomed Eng</stitle><addtitle>Nat Biomed Eng</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>4</volume><issue>12</issue><spage>1188</spage><epage>1196</epage><pages>1188-1196</pages><issn>2157-846X</issn><eissn>2157-846X</eissn><abstract>Accurate assays for the detection of antibodies to SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) are essential for the control of the COVID-19 (coronavirus disease 2019) pandemic. Here, we report antibody and antibody-avidity assays, relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates, for the simultaneous detection of antibodies to the S1 subunit of the spike protein and to the receptor binding domain of SARS-CoV-2 in human serum and saliva, and for quantifying immunoglobulin avidities against coronavirus antigens from SARS-CoV-2, SARS-CoV-1 and the common-cold viruses OC43, HKU1, NL63 and 229E. The antibody assay detected immunoglobulin M in 87% (52 of 60) COVID-19-positive serum samples collected 6 or more days after symptom onset (and the immunoglobulins M and G in all 33 samples collected at least 15 days after symptom onset), and correctly classified 456 out of the 457 COVID-19-negative serum samples tested (424 of them collected before the pandemic, including 73 that were positive for other viruses). We used the antibody-avidity assay to study antibody-maturation patterns, anamnestic responses, and cross-immunity to the common-cold coronaviruses.
Antibody and antibody-avidity assays relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates accurately quantify antibodies to SARS-CoV-2 and to common viruses in human serum and saliva.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33122853</pmid><doi>10.1038/s41551-020-00642-4</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-3378-8071</orcidid><orcidid>https://orcid.org/0000-0002-6707-1597</orcidid><orcidid>https://orcid.org/0000-0002-8541-5752</orcidid><orcidid>https://orcid.org/0000-0003-3432-4479</orcidid><orcidid>https://orcid.org/0000-0002-4906-4502</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 38/79 42/34 631/61/32 692/699/255/2514 82/1 96/47 Aged Amplification Antibodies Antibodies, Viral - blood Antibodies, Viral - immunology Antibody Affinity - immunology Antigens Antigens, Viral - immunology Assaying Avidity Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Coronaviridae Coronaviruses COVID-19 COVID-19 - blood COVID-19 - immunology COVID-19 Serological Testing - methods Female Fluorescence Gold Humans I.R. radiation Immunoglobulin G - blood Immunoglobulin G - immunology Immunoglobulin M Immunoglobulin M - blood Immunoglobulin M - immunology Immunoglobulins Nanostructure Pandemics Pandemics - prevention & control Plasmonics Saliva Saliva - immunology SARS-CoV-2 - immunology Severe acute respiratory syndrome coronavirus 2 Spike protein Substrates Viral diseases Viruses |
| title | Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates |
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