“One-step” characterization platform for pathogenic genetics of Staphylococcus aureus
Staphylococcus aureus ( S. aureus ) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by S. aureus contaminated food has become a global health problem. S. aureus can express various pathogenic factors, mainly used for adhesion,...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2021-05, Vol.44 (5), p.985-994 |
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| creator | Li, Yanmei Qiu, Yisen Ye, Congxiu Chen, Ling Liang, Yi Huang, Teng-Yi Zhang, Li Liu, Junyan |
| description | Staphylococcus aureus
(
S. aureus
) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by
S. aureus
contaminated food has become a global health problem.
S. aureus
can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in
S. aureus
is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in
S. aureus
, including
sea
,
seb
,
sec
,
see
,
pvl
and
tsst
, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in
S. aureus
. |
| doi_str_mv | 10.1007/s00449-020-02449-8 |
| format | Article |
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(
S. aureus
) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by
S. aureus
contaminated food has become a global health problem.
S. aureus
can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in
S. aureus
is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in
S. aureus
, including
sea
,
seb
,
sec
,
see
,
pvl
and
tsst
, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in
S. aureus
.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-020-02449-8</identifier><identifier>PMID: 33112989</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biotechnology ; Chemistry ; Chemistry and Materials Science ; Colonization ; Environmental Engineering/Biotechnology ; Food contamination ; Food Science ; Foodborne diseases ; Foodborne pathogens ; Genes ; Genetics ; Global health ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Pathogens ; Penicillin ; Public health ; Research Paper ; Staphylococcus aureus ; Toxins ; Virulence</subject><ispartof>Bioprocess and biosystems engineering, 2021-05, Vol.44 (5), p.985-994</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-7da9e0a57ffd3cd32e983d6f272fe19318552139d461feca2cd524948679a8983</citedby><cites>FETCH-LOGICAL-c412t-7da9e0a57ffd3cd32e983d6f272fe19318552139d461feca2cd524948679a8983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-020-02449-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-020-02449-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33112989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yanmei</creatorcontrib><creatorcontrib>Qiu, Yisen</creatorcontrib><creatorcontrib>Ye, Congxiu</creatorcontrib><creatorcontrib>Chen, Ling</creatorcontrib><creatorcontrib>Liang, Yi</creatorcontrib><creatorcontrib>Huang, Teng-Yi</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Liu, Junyan</creatorcontrib><title>“One-step” characterization platform for pathogenic genetics of Staphylococcus aureus</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Staphylococcus aureus
(
S. aureus
) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by
S. aureus
contaminated food has become a global health problem.
S. aureus
can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in
S. aureus
is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in
S. aureus
, including
sea
,
seb
,
sec
,
see
,
pvl
and
tsst
, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in
S. aureus
.</description><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colonization</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Food contamination</subject><subject>Food Science</subject><subject>Foodborne diseases</subject><subject>Foodborne pathogens</subject><subject>Genes</subject><subject>Genetics</subject><subject>Global health</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Pathogens</subject><subject>Penicillin</subject><subject>Public health</subject><subject>Research Paper</subject><subject>Staphylococcus aureus</subject><subject>Toxins</subject><subject>Virulence</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kLtOBCEUhonR6Hp5AQsziY3NKDAwA6XZeEtMLNTCiiBzcMfMDiMwxVrtg-jL7ZPIul4SCwsOJ-HjPycfQvsEHxOMq5OAMWMyxxSns-zEGhqRkvC8KjFf_-65JFtoO4RnjAkXFG-iraIghEohR-hhMX-76SAPEfrF_D0zE-21ieCbVx0b12V9q6N1fpqlkvU6TtwTdI3JUoXYmJA5m91G3U9mrTPOmCFkevAwhF20YXUbYO_r3kH352d348v8-ubianx6nRtGaMyrWkvAmlfW1oWpCwpSFHVpaUUtEFkQwTklhaxZSSwYTU3NKZNMlJXUIrE76GiV23v3MkCIatoEA22rO3BDUJRxLhimkib08A_67Abfpe0UTUMEqYhgiaIryngXggeret9MtZ8pgtVSvFqJV0m8-hSvllscfEUPj1Oof758m05AsQJCeuqewP_O_if2A_DYkA0</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Li, Yanmei</creator><creator>Qiu, Yisen</creator><creator>Ye, Congxiu</creator><creator>Chen, Ling</creator><creator>Liang, Yi</creator><creator>Huang, Teng-Yi</creator><creator>Zhang, Li</creator><creator>Liu, Junyan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20210501</creationdate><title>“One-step” characterization platform for pathogenic genetics of Staphylococcus aureus</title><author>Li, Yanmei ; Qiu, Yisen ; Ye, Congxiu ; Chen, Ling ; Liang, Yi ; Huang, Teng-Yi ; Zhang, Li ; Liu, Junyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-7da9e0a57ffd3cd32e983d6f272fe19318552139d461feca2cd524948679a8983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colonization</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Food contamination</topic><topic>Food Science</topic><topic>Foodborne diseases</topic><topic>Foodborne pathogens</topic><topic>Genes</topic><topic>Genetics</topic><topic>Global health</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Pathogens</topic><topic>Penicillin</topic><topic>Public health</topic><topic>Research Paper</topic><topic>Staphylococcus aureus</topic><topic>Toxins</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yanmei</creatorcontrib><creatorcontrib>Qiu, Yisen</creatorcontrib><creatorcontrib>Ye, Congxiu</creatorcontrib><creatorcontrib>Chen, Ling</creatorcontrib><creatorcontrib>Liang, Yi</creatorcontrib><creatorcontrib>Huang, Teng-Yi</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Liu, Junyan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yanmei</au><au>Qiu, Yisen</au><au>Ye, Congxiu</au><au>Chen, Ling</au><au>Liang, Yi</au><au>Huang, Teng-Yi</au><au>Zhang, Li</au><au>Liu, Junyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>“One-step” characterization platform for pathogenic genetics of Staphylococcus aureus</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>44</volume><issue>5</issue><spage>985</spage><epage>994</epage><pages>985-994</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>Staphylococcus aureus
(
S. aureus
) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by
S. aureus
contaminated food has become a global health problem.
S. aureus
can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in
S. aureus
is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in
S. aureus
, including
sea
,
seb
,
sec
,
see
,
pvl
and
tsst
, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in
S. aureus
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33112989</pmid><doi>10.1007/s00449-020-02449-8</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1615-7591 |
| ispartof | Bioprocess and biosystems engineering, 2021-05, Vol.44 (5), p.985-994 |
| issn | 1615-7591 1615-7605 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2455840292 |
| source | Springer Online Journals Complete |
| subjects | Biotechnology Chemistry Chemistry and Materials Science Colonization Environmental Engineering/Biotechnology Food contamination Food Science Foodborne diseases Foodborne pathogens Genes Genetics Global health Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Pathogens Penicillin Public health Research Paper Staphylococcus aureus Toxins Virulence |
| title | “One-step” characterization platform for pathogenic genetics of Staphylococcus aureus |
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