Rapid Serotyping of Salmonella Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a Salmonella- Specific Gene
Although serotyping is the most important method of identification of taxonomy in , conventional serotype determination with a complete set of antisera is time consuming and laborious. Recently, rapid serotyping procedures with polymerase chain reaction (PCR) have been developed. In this study, we e...
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| Veröffentlicht in: | Foodborne pathogens and disease 2021-01, Vol.18 (1), p.31-40 |
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| creator | Yamasaki, Eiki Matsuzawa, Shigeru Takeuchi, Kaoru Morimoto, Yo Ikeda, Tetsuya Okumura, Kayo Kurazono, Hisao |
| description | Although serotyping is the most important method of identification of taxonomy in
, conventional serotype determination with a complete set of antisera is time consuming and laborious. Recently, rapid serotyping procedures with polymerase chain reaction (PCR) have been developed. In this study, we established a novel PCR-based rapid serotyping method that employs a unique target gene. Alignment study of
-specific gene (
enterotoxin [
]) revealed a correlation between the
gene sequence and the serotype of the organism. In 750 bp of
gene, 55 nucleotides indicated single nucleotide polymorphism (SNP)-like polymorphism, and the correlation between the SNP-like polymorphism and the serotype of the organism suggests that SNP-like sequences in
gene can serve as an index for serotyping. To develop a rapid serotyping method based on the SNP-like polymorphism, we selected serotype-associated 12 SNP-like sites in the
gene and established a method based on high-resolution melting (HRM) and PCR, which identifies nucleotides at SNP-like sites within 1.5 h. This newly established rapid serotyping procedure (
-HRM) could identify nine serotypes, including the frequently isolated serovar Enteritidis. These nine serotypes cover 64.3% of cases of
, as reported by the World Health Organization/Global Foodborne Infection Network (WHO/GFN) Country Databank from 2001 to 2010. In this study, we employed a unique target gene,
, which is completely independent of the genes that were targeted in previously reported rapid serotyping procedures. Therefore, the results obtained by our newly developed
-HRM procedure are independent of the results obtained by other procedures. Besides,
-HRM can ensure accurate identification of the bacterial species as
is a
-specific gene. It is expected that the combination of newly constructed
-HRM and previously reported procedures could further improve the credibility of
isolate serotyping. |
| doi_str_mv | 10.1089/fpd.2020.2823 |
| format | Article |
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, conventional serotype determination with a complete set of antisera is time consuming and laborious. Recently, rapid serotyping procedures with polymerase chain reaction (PCR) have been developed. In this study, we established a novel PCR-based rapid serotyping method that employs a unique target gene. Alignment study of
-specific gene (
enterotoxin [
]) revealed a correlation between the
gene sequence and the serotype of the organism. In 750 bp of
gene, 55 nucleotides indicated single nucleotide polymorphism (SNP)-like polymorphism, and the correlation between the SNP-like polymorphism and the serotype of the organism suggests that SNP-like sequences in
gene can serve as an index for serotyping. To develop a rapid serotyping method based on the SNP-like polymorphism, we selected serotype-associated 12 SNP-like sites in the
gene and established a method based on high-resolution melting (HRM) and PCR, which identifies nucleotides at SNP-like sites within 1.5 h. This newly established rapid serotyping procedure (
-HRM) could identify nine serotypes, including the frequently isolated serovar Enteritidis. These nine serotypes cover 64.3% of cases of
, as reported by the World Health Organization/Global Foodborne Infection Network (WHO/GFN) Country Databank from 2001 to 2010. In this study, we employed a unique target gene,
, which is completely independent of the genes that were targeted in previously reported rapid serotyping procedures. Therefore, the results obtained by our newly developed
-HRM procedure are independent of the results obtained by other procedures. Besides,
-HRM can ensure accurate identification of the bacterial species as
is a
-specific gene. It is expected that the combination of newly constructed
-HRM and previously reported procedures could further improve the credibility of
isolate serotyping.</description><identifier>ISSN: 1535-3141</identifier><identifier>EISSN: 1556-7125</identifier><identifier>DOI: 10.1089/fpd.2020.2823</identifier><identifier>PMID: 33103921</identifier><language>eng</language><publisher>United States</publisher><ispartof>Foodborne pathogens and disease, 2021-01, Vol.18 (1), p.31-40</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-c62f043ba0eaeca0e9c133ccb7e4503e26bfb53df678699ccc8b37c00b53ad193</citedby><cites>FETCH-LOGICAL-c359t-c62f043ba0eaeca0e9c133ccb7e4503e26bfb53df678699ccc8b37c00b53ad193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33103921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamasaki, Eiki</creatorcontrib><creatorcontrib>Matsuzawa, Shigeru</creatorcontrib><creatorcontrib>Takeuchi, Kaoru</creatorcontrib><creatorcontrib>Morimoto, Yo</creatorcontrib><creatorcontrib>Ikeda, Tetsuya</creatorcontrib><creatorcontrib>Okumura, Kayo</creatorcontrib><creatorcontrib>Kurazono, Hisao</creatorcontrib><title>Rapid Serotyping of Salmonella Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a Salmonella- Specific Gene</title><title>Foodborne pathogens and disease</title><addtitle>Foodborne Pathog Dis</addtitle><description>Although serotyping is the most important method of identification of taxonomy in
, conventional serotype determination with a complete set of antisera is time consuming and laborious. Recently, rapid serotyping procedures with polymerase chain reaction (PCR) have been developed. In this study, we established a novel PCR-based rapid serotyping method that employs a unique target gene. Alignment study of
-specific gene (
enterotoxin [
]) revealed a correlation between the
gene sequence and the serotype of the organism. In 750 bp of
gene, 55 nucleotides indicated single nucleotide polymorphism (SNP)-like polymorphism, and the correlation between the SNP-like polymorphism and the serotype of the organism suggests that SNP-like sequences in
gene can serve as an index for serotyping. To develop a rapid serotyping method based on the SNP-like polymorphism, we selected serotype-associated 12 SNP-like sites in the
gene and established a method based on high-resolution melting (HRM) and PCR, which identifies nucleotides at SNP-like sites within 1.5 h. This newly established rapid serotyping procedure (
-HRM) could identify nine serotypes, including the frequently isolated serovar Enteritidis. These nine serotypes cover 64.3% of cases of
, as reported by the World Health Organization/Global Foodborne Infection Network (WHO/GFN) Country Databank from 2001 to 2010. In this study, we employed a unique target gene,
, which is completely independent of the genes that were targeted in previously reported rapid serotyping procedures. Therefore, the results obtained by our newly developed
-HRM procedure are independent of the results obtained by other procedures. Besides,
-HRM can ensure accurate identification of the bacterial species as
is a
-specific gene. It is expected that the combination of newly constructed
-HRM and previously reported procedures could further improve the credibility of
isolate serotyping.</description><issn>1535-3141</issn><issn>1556-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpNkE1PwyAYgInRuDk9ejUcvXTyUfpx1EXnkkWN03ND6YuitNTSHvYD_N_SbBovQODhyZsHoXNK5pRk-ZVuqzkjjMxZxvgBmlIhkiilTByOZy4iTmM6QSfefxDCcibSYzThnBKeMzpF38-yNRXeQOf6bWuaN-w03khbuwaslXjlnZU9eHwjPVTYNXgTIAv4YVAWXG8qwE_ObmvXte_G19HafELQfQ3QqPDUOW1s-B6s8p83wpsWlNFG4SU0cIqOtLQezvb7DL3e3b4s7qP143K1uF5Hiou8j1TCNIl5KQlIUGHNFeVcqTKFWBAOLCl1KXilkzRL8lwplZU8VYSES1nRnM_Q5c7bdi5M6PuiNl6NAzXgBl-wWMQxSUSWBTTaoapz3negi7Yztey2BSXFWL4I5YuxfDGWD_zFXj2UNVR_9G9q_gNdn4D3</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Yamasaki, Eiki</creator><creator>Matsuzawa, Shigeru</creator><creator>Takeuchi, Kaoru</creator><creator>Morimoto, Yo</creator><creator>Ikeda, Tetsuya</creator><creator>Okumura, Kayo</creator><creator>Kurazono, Hisao</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202101</creationdate><title>Rapid Serotyping of Salmonella Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a Salmonella- Specific Gene</title><author>Yamasaki, Eiki ; Matsuzawa, Shigeru ; Takeuchi, Kaoru ; Morimoto, Yo ; Ikeda, Tetsuya ; Okumura, Kayo ; Kurazono, Hisao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-c62f043ba0eaeca0e9c133ccb7e4503e26bfb53df678699ccc8b37c00b53ad193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamasaki, Eiki</creatorcontrib><creatorcontrib>Matsuzawa, Shigeru</creatorcontrib><creatorcontrib>Takeuchi, Kaoru</creatorcontrib><creatorcontrib>Morimoto, Yo</creatorcontrib><creatorcontrib>Ikeda, Tetsuya</creatorcontrib><creatorcontrib>Okumura, Kayo</creatorcontrib><creatorcontrib>Kurazono, Hisao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Foodborne pathogens and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamasaki, Eiki</au><au>Matsuzawa, Shigeru</au><au>Takeuchi, Kaoru</au><au>Morimoto, Yo</au><au>Ikeda, Tetsuya</au><au>Okumura, Kayo</au><au>Kurazono, Hisao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Serotyping of Salmonella Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a Salmonella- Specific Gene</atitle><jtitle>Foodborne pathogens and disease</jtitle><addtitle>Foodborne Pathog Dis</addtitle><date>2021-01</date><risdate>2021</risdate><volume>18</volume><issue>1</issue><spage>31</spage><epage>40</epage><pages>31-40</pages><issn>1535-3141</issn><eissn>1556-7125</eissn><abstract>Although serotyping is the most important method of identification of taxonomy in
, conventional serotype determination with a complete set of antisera is time consuming and laborious. Recently, rapid serotyping procedures with polymerase chain reaction (PCR) have been developed. In this study, we established a novel PCR-based rapid serotyping method that employs a unique target gene. Alignment study of
-specific gene (
enterotoxin [
]) revealed a correlation between the
gene sequence and the serotype of the organism. In 750 bp of
gene, 55 nucleotides indicated single nucleotide polymorphism (SNP)-like polymorphism, and the correlation between the SNP-like polymorphism and the serotype of the organism suggests that SNP-like sequences in
gene can serve as an index for serotyping. To develop a rapid serotyping method based on the SNP-like polymorphism, we selected serotype-associated 12 SNP-like sites in the
gene and established a method based on high-resolution melting (HRM) and PCR, which identifies nucleotides at SNP-like sites within 1.5 h. This newly established rapid serotyping procedure (
-HRM) could identify nine serotypes, including the frequently isolated serovar Enteritidis. These nine serotypes cover 64.3% of cases of
, as reported by the World Health Organization/Global Foodborne Infection Network (WHO/GFN) Country Databank from 2001 to 2010. In this study, we employed a unique target gene,
, which is completely independent of the genes that were targeted in previously reported rapid serotyping procedures. Therefore, the results obtained by our newly developed
-HRM procedure are independent of the results obtained by other procedures. Besides,
-HRM can ensure accurate identification of the bacterial species as
is a
-specific gene. It is expected that the combination of newly constructed
-HRM and previously reported procedures could further improve the credibility of
isolate serotyping.</abstract><cop>United States</cop><pmid>33103921</pmid><doi>10.1089/fpd.2020.2823</doi><tpages>10</tpages></addata></record> |
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| source | Alma/SFX Local Collection |
| title | Rapid Serotyping of Salmonella Isolates Based on Single Nucleotide Polymorphism-Like Sequence Profiles of a Salmonella- Specific Gene |
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