A rheological approach to identify efficient biopolymer producing bacteria

This study investigates the relationship between collective motion and propulsion of bacterial consortia and their biopolymer production efficiency. Rheological tests were conducted for suspensions of two different methanotrophic bacterial consortia obtained after enrichment of sediment samples from mangrove sites in Brazil. We considered the linear viscoelasticity region and analyzed the values of storage and loss moduli as functions of days of cultivation, for different values of the volume fraction. The suspensions' rheological behaviors reflected the bacterial growth stage. We found that the formation of structures over time in some types of consortia can hinder the movement of bacteria in the search for nutrients. The change in complex viscosity of the two consortia followed a different and rich behavior that appears to be closely related to their capacity to capture methane. Our analysis showed a possible correlation between collective motion, viscosity reduction, and biopolymer production. The pieces of evidence from this study suggest that the efficiency of bacterial motion is directly related to biopolymer production, and this could facilitate the process of identifying the best consortium of biopolymer producing bacteria. A novel analysis using rheological characterization is conducted in this study using two different consortia of biopolymer producing bacteria. Malvar and coworkers' findings support the idea that consortia formed by bacteria with better propulsion efficiency or those in which the forming bacteria interact constructively, that is, generating a negative stresslet strength, may favor PHB accumulation. By using this strategy, consortia capable of efficiently produce a biopolymer is identified.