Antifungal activity and potential mechanism of magnoflorine against Trichophyton rubrum
Coptis alkaloids show potent antifungal activity against Trichophyton rubrum (T. rubrum) , which was a Tinea pedis fungus, but little of the literature was reported to investigate the antifungal activity of magnoflorine against it. Meanwhile, the potential mechanism of magnoflorine against T. rubrum...
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| Veröffentlicht in: | Journal of antibiotics 2021-03, Vol.74 (3), p.206-214 |
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| creator | Luo, Nanxuan Jin, Liang Yang, Changqing Zhu, Yurong Ye, Xiaoli Li, Xuegang Zhang, Baoshun |
| description | Coptis alkaloids show potent antifungal activity against
Trichophyton rubrum (T. rubrum)
, which was a Tinea pedis fungus, but little of the literature was reported to investigate the antifungal activity of magnoflorine against it. Meanwhile, the potential mechanism of magnoflorine against
T. rubrum
is unknown. In the present study, we found that Coptis alkaloids, especially magnoflorine had significant antifungal activities against
T. rubrum
and
Trichophyton mentagrophyte (T. mentagrophyte)
. The MIC values of magnoflorine against
T. rubrum
and
T. mentagrophyte
were both 62.5 μg ml
−1
, but magnoflorine exerted a better fungicidal efficiency against
T. rubrum
than
T. mentagrophyte
. Magnoflorine inhibited the conidia germination and hyphal growth, and changed the mycelial morphology such as deformation growth, surface peeling, and cytoplasmic contraction in
T. rubrum
. Magnoflorine had no significant effect on cell wall integrity. However, magnoflorine destroyed the fungal cell membrane of
T. rubrum
through increasing the nucleic acid leakage, reducing the activities of squalene epoxidase and CYP51 enzyme, and decreasing the content of ergosterol in hyphae. Our study supported the potential use of magnoflorine as an antifungal agent against
T. rubrum
and made contributions to the clinical application of magnoflorine against fungi. |
| doi_str_mv | 10.1038/s41429-020-00380-4 |
| format | Article |
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Trichophyton rubrum (T. rubrum)
, which was a Tinea pedis fungus, but little of the literature was reported to investigate the antifungal activity of magnoflorine against it. Meanwhile, the potential mechanism of magnoflorine against
T. rubrum
is unknown. In the present study, we found that Coptis alkaloids, especially magnoflorine had significant antifungal activities against
T. rubrum
and
Trichophyton mentagrophyte (T. mentagrophyte)
. The MIC values of magnoflorine against
T. rubrum
and
T. mentagrophyte
were both 62.5 μg ml
−1
, but magnoflorine exerted a better fungicidal efficiency against
T. rubrum
than
T. mentagrophyte
. Magnoflorine inhibited the conidia germination and hyphal growth, and changed the mycelial morphology such as deformation growth, surface peeling, and cytoplasmic contraction in
T. rubrum
. Magnoflorine had no significant effect on cell wall integrity. However, magnoflorine destroyed the fungal cell membrane of
T. rubrum
through increasing the nucleic acid leakage, reducing the activities of squalene epoxidase and CYP51 enzyme, and decreasing the content of ergosterol in hyphae. Our study supported the potential use of magnoflorine as an antifungal agent against
T. rubrum
and made contributions to the clinical application of magnoflorine against fungi.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.1038/s41429-020-00380-4</identifier><identifier>PMID: 33082529</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14 ; 14/28 ; 631/378/193 ; 692/699 ; Antibiotics ; Antifungal agents ; Athletes foot ; Bacteriology ; Biomedical and Life Sciences ; Bioorganic Chemistry ; Drinking water ; Fungi ; Life Sciences ; Medicinal Chemistry ; Microbiology ; Morphology ; Nucleic acids ; Organic Chemistry</subject><ispartof>Journal of antibiotics, 2021-03, Vol.74 (3), p.206-214</ispartof><rights>The Author(s), under exclusive licence to the Japan Antibiotics Research Association 2020</rights><rights>The Author(s), under exclusive licence to the Japan Antibiotics Research Association 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3144-a3c80c0e9603d50ea05feaaac783def358548a290067456abeb6976daf8461143</citedby><cites>FETCH-LOGICAL-c3144-a3c80c0e9603d50ea05feaaac783def358548a290067456abeb6976daf8461143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33082529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Nanxuan</creatorcontrib><creatorcontrib>Jin, Liang</creatorcontrib><creatorcontrib>Yang, Changqing</creatorcontrib><creatorcontrib>Zhu, Yurong</creatorcontrib><creatorcontrib>Ye, Xiaoli</creatorcontrib><creatorcontrib>Li, Xuegang</creatorcontrib><creatorcontrib>Zhang, Baoshun</creatorcontrib><title>Antifungal activity and potential mechanism of magnoflorine against Trichophyton rubrum</title><title>Journal of antibiotics</title><addtitle>J Antibiot</addtitle><addtitle>J Antibiot (Tokyo)</addtitle><description>Coptis alkaloids show potent antifungal activity against
Trichophyton rubrum (T. rubrum)
, which was a Tinea pedis fungus, but little of the literature was reported to investigate the antifungal activity of magnoflorine against it. Meanwhile, the potential mechanism of magnoflorine against
T. rubrum
is unknown. In the present study, we found that Coptis alkaloids, especially magnoflorine had significant antifungal activities against
T. rubrum
and
Trichophyton mentagrophyte (T. mentagrophyte)
. The MIC values of magnoflorine against
T. rubrum
and
T. mentagrophyte
were both 62.5 μg ml
−1
, but magnoflorine exerted a better fungicidal efficiency against
T. rubrum
than
T. mentagrophyte
. Magnoflorine inhibited the conidia germination and hyphal growth, and changed the mycelial morphology such as deformation growth, surface peeling, and cytoplasmic contraction in
T. rubrum
. Magnoflorine had no significant effect on cell wall integrity. However, magnoflorine destroyed the fungal cell membrane of
T. rubrum
through increasing the nucleic acid leakage, reducing the activities of squalene epoxidase and CYP51 enzyme, and decreasing the content of ergosterol in hyphae. Our study supported the potential use of magnoflorine as an antifungal agent against
T. rubrum
and made contributions to the clinical application of magnoflorine against fungi.</description><subject>14</subject><subject>14/28</subject><subject>631/378/193</subject><subject>692/699</subject><subject>Antibiotics</subject><subject>Antifungal agents</subject><subject>Athletes foot</subject><subject>Bacteriology</subject><subject>Biomedical and Life Sciences</subject><subject>Bioorganic Chemistry</subject><subject>Drinking water</subject><subject>Fungi</subject><subject>Life Sciences</subject><subject>Medicinal Chemistry</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Nucleic acids</subject><subject>Organic Chemistry</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kMtKxDAUhoMoOl5ewIUU3LipnlzapksRbyC4UVyGM2k6k6FNxqQV5u3NOKOCCyEQkvPlPycfIacULilweRUFFazOgUEO6Qy52CETKiXNqSjrXTIBYDSXksEBOYxxkaCKV3KfHHAOkhWsnpC3azfYdnQz7DLUg_2wwypD12RLP5hUSte90XN0NvaZb7MeZ863nQ_WmQxnaF0cspdg9dwv56vBuyyM0zD2x2SvxS6ak-1-RF7vbl9uHvKn5_vHm-unXHMqRI5cS9Bg6hJ4U4BBKFqDiLqSvDEtL2QhJLIaoKxEUeLUTMu6KhtspSgpFfyIXGxyl8G_jyYOqrdRm65DZ_wYFRPpm1VdSkjo-R904cfg0nSJkhUteFqJYhtKBx9jMK1aBttjWCkKaq1dbbSrpF19aVfrKc620eO0N83Pk2_PCeAbIKaSm5nw2_uf2E_nF42d</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Luo, Nanxuan</creator><creator>Jin, Liang</creator><creator>Yang, Changqing</creator><creator>Zhu, Yurong</creator><creator>Ye, Xiaoli</creator><creator>Li, Xuegang</creator><creator>Zhang, Baoshun</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20210301</creationdate><title>Antifungal activity and potential mechanism of magnoflorine against Trichophyton rubrum</title><author>Luo, Nanxuan ; 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Trichophyton rubrum (T. rubrum)
, which was a Tinea pedis fungus, but little of the literature was reported to investigate the antifungal activity of magnoflorine against it. Meanwhile, the potential mechanism of magnoflorine against
T. rubrum
is unknown. In the present study, we found that Coptis alkaloids, especially magnoflorine had significant antifungal activities against
T. rubrum
and
Trichophyton mentagrophyte (T. mentagrophyte)
. The MIC values of magnoflorine against
T. rubrum
and
T. mentagrophyte
were both 62.5 μg ml
−1
, but magnoflorine exerted a better fungicidal efficiency against
T. rubrum
than
T. mentagrophyte
. Magnoflorine inhibited the conidia germination and hyphal growth, and changed the mycelial morphology such as deformation growth, surface peeling, and cytoplasmic contraction in
T. rubrum
. Magnoflorine had no significant effect on cell wall integrity. However, magnoflorine destroyed the fungal cell membrane of
T. rubrum
through increasing the nucleic acid leakage, reducing the activities of squalene epoxidase and CYP51 enzyme, and decreasing the content of ergosterol in hyphae. Our study supported the potential use of magnoflorine as an antifungal agent against
T. rubrum
and made contributions to the clinical application of magnoflorine against fungi.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33082529</pmid><doi>10.1038/s41429-020-00380-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of antibiotics, 2021-03, Vol.74 (3), p.206-214 |
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| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | 14 14/28 631/378/193 692/699 Antibiotics Antifungal agents Athletes foot Bacteriology Biomedical and Life Sciences Bioorganic Chemistry Drinking water Fungi Life Sciences Medicinal Chemistry Microbiology Morphology Nucleic acids Organic Chemistry |
| title | Antifungal activity and potential mechanism of magnoflorine against Trichophyton rubrum |
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