IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.)
Key message The transcription factor (TF) IbERF71 forms a novel complex, IbERF71-IbMYB340-IbbHLH2 , to coregulate anthocyanin biosynthesis by binding to the IbANS1 promoter in purple-fleshed sweet potatoes. Purple-fleshed sweet potato ( Ipomoea batatas L.) is very popular because of its abundant ant...
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| Veröffentlicht in: | Plant cell reports 2021, Vol.40 (1), p.157-169 |
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| creator | Ning, Zhiyuan Hu, Kangdi Zhou, Zhilin Zhao, Donglan Tang, Jun Wang, Hong Li, Lixia Ding, Chen Chen, Xiaoyan Yao, Gaifang Zhang, Hua |
| description | Key message
The transcription factor (TF)
IbERF71
forms a novel complex,
IbERF71-IbMYB340-IbbHLH2
, to coregulate anthocyanin biosynthesis by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes.
Purple-fleshed sweet potato (
Ipomoea batatas
L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TF
IbERF71
and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels of
IbERF71
and partners
IbMYB340
and
IbbHLH2
were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation of
IbMYB340+IbbHLH2
resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additional
IbERF71
significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels of
FvANS
and
FvGST
, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity of
IbANS1.
In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes. |
| doi_str_mv | 10.1007/s00299-020-02621-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2452977465</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2478285759</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2900-72cbf911824d6c1bcac69d63799ea24066e619b8070fe76e9c858a0940c30dbf3</originalsourceid><addsrcrecordid>eNp9kc9u1DAQxi0EokvhBTggS1yK1JSxk9jxsVQtu9ICEn8kOEW2M9lNlY2Dnajal-mzdsoWkDhwsKyZ-X3fjPQx9lLAmQDQbxOANCYDCfSUFBk8YgtR5DKTkH9_zBagqam1KI7Ys5SuAWio1VN2lOdQFUaVC3a7cpefr7Q45TfdtOUr9-HHu7wAboeGCrdcL-Up9yHiZu7thIkG0zb4vR26gVvv5919vwsDd3vuuqHphg2fAp-2SPrzj18EH2PYhQkjJ8U4x7HHrO0xbbHh6QZx4mOYLElOViOBaLmzVNvE12dvnrMnre0Tvnj4j9m3q8uvF8ts_en96uJ8nXlpADItvWuNEJUsGuWF89Yr06hcG4NWFqAUKmFcBRpa1AqNr8rKginA59C4Nj9mJwdfOvbnjGmqd13y2Pd2wDCnWhalNFoXqiT09T_odZjjQNcRpStZlbo0RMkD5WNIKWJbj7Hb2bivBdT36dWH9GpKr_6VXg0kevVgPbsdNn8kv-MiID8AiUbDBuPf3f-xvQMq0qMh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2478285759</pqid></control><display><type>article</type><title>IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.)</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Ning, Zhiyuan ; Hu, Kangdi ; Zhou, Zhilin ; Zhao, Donglan ; Tang, Jun ; Wang, Hong ; Li, Lixia ; Ding, Chen ; Chen, Xiaoyan ; Yao, Gaifang ; Zhang, Hua</creator><creatorcontrib>Ning, Zhiyuan ; Hu, Kangdi ; Zhou, Zhilin ; Zhao, Donglan ; Tang, Jun ; Wang, Hong ; Li, Lixia ; Ding, Chen ; Chen, Xiaoyan ; Yao, Gaifang ; Zhang, Hua</creatorcontrib><description>Key message
The transcription factor (TF)
IbERF71
forms a novel complex,
IbERF71-IbMYB340-IbbHLH2
, to coregulate anthocyanin biosynthesis by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes.
Purple-fleshed sweet potato (
Ipomoea batatas
L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TF
IbERF71
and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels of
IbERF71
and partners
IbMYB340
and
IbbHLH2
were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation of
IbMYB340+IbbHLH2
resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additional
IbERF71
significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels of
FvANS
and
FvGST
, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity of
IbANS1.
In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-020-02621-0</identifier><identifier>PMID: 33084965</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Anthocyanins ; Anthocyanins - genetics ; Anthocyanins - metabolism ; Binding ; Bioinformatics ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Cell Biology ; Fragaria - genetics ; Fragaria - metabolism ; Gene Expression Regulation, Plant ; Ipomoea batatas ; Ipomoea batatas - genetics ; Ipomoea batatas - metabolism ; Life Sciences ; Nicotiana - genetics ; Nicotiana - metabolism ; Original Article ; Pigmentation ; Pigments ; Plant Biochemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; Plants, Genetically Modified ; Potatoes ; Promoter Regions, Genetic ; Protein Interaction Maps ; Strawberries ; Sweet potatoes ; Tobacco ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Vegetables ; Visual aspects ; Yeasts</subject><ispartof>Plant cell reports, 2021, Vol.40 (1), p.157-169</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2900-72cbf911824d6c1bcac69d63799ea24066e619b8070fe76e9c858a0940c30dbf3</citedby><cites>FETCH-LOGICAL-c2900-72cbf911824d6c1bcac69d63799ea24066e619b8070fe76e9c858a0940c30dbf3</cites><orcidid>0000-0002-8406-8373</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-020-02621-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-020-02621-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33084965$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ning, Zhiyuan</creatorcontrib><creatorcontrib>Hu, Kangdi</creatorcontrib><creatorcontrib>Zhou, Zhilin</creatorcontrib><creatorcontrib>Zhao, Donglan</creatorcontrib><creatorcontrib>Tang, Jun</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>Li, Lixia</creatorcontrib><creatorcontrib>Ding, Chen</creatorcontrib><creatorcontrib>Chen, Xiaoyan</creatorcontrib><creatorcontrib>Yao, Gaifang</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><title>IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Key message
The transcription factor (TF)
IbERF71
forms a novel complex,
IbERF71-IbMYB340-IbbHLH2
, to coregulate anthocyanin biosynthesis by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes.
Purple-fleshed sweet potato (
Ipomoea batatas
L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TF
IbERF71
and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels of
IbERF71
and partners
IbMYB340
and
IbbHLH2
were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation of
IbMYB340+IbbHLH2
resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additional
IbERF71
significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels of
FvANS
and
FvGST
, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity of
IbANS1.
In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes.</description><subject>Accumulation</subject><subject>Anthocyanins</subject><subject>Anthocyanins - genetics</subject><subject>Anthocyanins - metabolism</subject><subject>Binding</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Fragaria - genetics</subject><subject>Fragaria - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Ipomoea batatas</subject><subject>Ipomoea batatas - genetics</subject><subject>Ipomoea batatas - metabolism</subject><subject>Life Sciences</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Original Article</subject><subject>Pigmentation</subject><subject>Pigments</subject><subject>Plant Biochemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified</subject><subject>Potatoes</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Interaction Maps</subject><subject>Strawberries</subject><subject>Sweet potatoes</subject><subject>Tobacco</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Vegetables</subject><subject>Visual aspects</subject><subject>Yeasts</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc9u1DAQxi0EokvhBTggS1yK1JSxk9jxsVQtu9ICEn8kOEW2M9lNlY2Dnajal-mzdsoWkDhwsKyZ-X3fjPQx9lLAmQDQbxOANCYDCfSUFBk8YgtR5DKTkH9_zBagqam1KI7Ys5SuAWio1VN2lOdQFUaVC3a7cpefr7Q45TfdtOUr9-HHu7wAboeGCrdcL-Up9yHiZu7thIkG0zb4vR26gVvv5919vwsDd3vuuqHphg2fAp-2SPrzj18EH2PYhQkjJ8U4x7HHrO0xbbHh6QZx4mOYLElOViOBaLmzVNvE12dvnrMnre0Tvnj4j9m3q8uvF8ts_en96uJ8nXlpADItvWuNEJUsGuWF89Yr06hcG4NWFqAUKmFcBRpa1AqNr8rKginA59C4Nj9mJwdfOvbnjGmqd13y2Pd2wDCnWhalNFoXqiT09T_odZjjQNcRpStZlbo0RMkD5WNIKWJbj7Hb2bivBdT36dWH9GpKr_6VXg0kevVgPbsdNn8kv-MiID8AiUbDBuPf3f-xvQMq0qMh</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Ning, Zhiyuan</creator><creator>Hu, Kangdi</creator><creator>Zhou, Zhilin</creator><creator>Zhao, Donglan</creator><creator>Tang, Jun</creator><creator>Wang, Hong</creator><creator>Li, Lixia</creator><creator>Ding, Chen</creator><creator>Chen, Xiaoyan</creator><creator>Yao, Gaifang</creator><creator>Zhang, Hua</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8406-8373</orcidid></search><sort><creationdate>2021</creationdate><title>IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.)</title><author>Ning, Zhiyuan ; Hu, Kangdi ; Zhou, Zhilin ; Zhao, Donglan ; Tang, Jun ; Wang, Hong ; Li, Lixia ; Ding, Chen ; Chen, Xiaoyan ; Yao, Gaifang ; Zhang, Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2900-72cbf911824d6c1bcac69d63799ea24066e619b8070fe76e9c858a0940c30dbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Accumulation</topic><topic>Anthocyanins</topic><topic>Anthocyanins - genetics</topic><topic>Anthocyanins - metabolism</topic><topic>Binding</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Fragaria - genetics</topic><topic>Fragaria - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Ipomoea batatas</topic><topic>Ipomoea batatas - genetics</topic><topic>Ipomoea batatas - metabolism</topic><topic>Life Sciences</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Original Article</topic><topic>Pigmentation</topic><topic>Pigments</topic><topic>Plant Biochemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified</topic><topic>Potatoes</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Interaction Maps</topic><topic>Strawberries</topic><topic>Sweet potatoes</topic><topic>Tobacco</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Vegetables</topic><topic>Visual aspects</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ning, Zhiyuan</creatorcontrib><creatorcontrib>Hu, Kangdi</creatorcontrib><creatorcontrib>Zhou, Zhilin</creatorcontrib><creatorcontrib>Zhao, Donglan</creatorcontrib><creatorcontrib>Tang, Jun</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>Li, Lixia</creatorcontrib><creatorcontrib>Ding, Chen</creatorcontrib><creatorcontrib>Chen, Xiaoyan</creatorcontrib><creatorcontrib>Yao, Gaifang</creatorcontrib><creatorcontrib>Zhang, Hua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ning, Zhiyuan</au><au>Hu, Kangdi</au><au>Zhou, Zhilin</au><au>Zhao, Donglan</au><au>Tang, Jun</au><au>Wang, Hong</au><au>Li, Lixia</au><au>Ding, Chen</au><au>Chen, Xiaoyan</au><au>Yao, Gaifang</au><au>Zhang, Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.)</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2021</date><risdate>2021</risdate><volume>40</volume><issue>1</issue><spage>157</spage><epage>169</epage><pages>157-169</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>Key message
The transcription factor (TF)
IbERF71
forms a novel complex,
IbERF71-IbMYB340-IbbHLH2
, to coregulate anthocyanin biosynthesis by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes.
Purple-fleshed sweet potato (
Ipomoea batatas
L.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TF
IbERF71
and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels of
IbERF71
and partners
IbMYB340
and
IbbHLH2
were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation of
IbMYB340+IbbHLH2
resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additional
IbERF71
significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels of
FvANS
and
FvGST
, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity of
IbANS1.
In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to the
IbANS1
promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33084965</pmid><doi>10.1007/s00299-020-02621-0</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-8406-8373</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 2021, Vol.40 (1), p.157-169 |
| issn | 0721-7714 1432-203X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2452977465 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Accumulation Anthocyanins Anthocyanins - genetics Anthocyanins - metabolism Binding Bioinformatics Biomedical and Life Sciences Biosynthesis Biotechnology Cell Biology Fragaria - genetics Fragaria - metabolism Gene Expression Regulation, Plant Ipomoea batatas Ipomoea batatas - genetics Ipomoea batatas - metabolism Life Sciences Nicotiana - genetics Nicotiana - metabolism Original Article Pigmentation Pigments Plant Biochemistry Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences Plants, Genetically Modified Potatoes Promoter Regions, Genetic Protein Interaction Maps Strawberries Sweet potatoes Tobacco Transcription Factors - genetics Transcription Factors - metabolism Vegetables Visual aspects Yeasts |
| title | IbERF71, with IbMYB340 and IbbHLH2, coregulates anthocyanin accumulation by binding to the IbANS1 promoter in purple-fleshed sweet potato (Ipomoea batatas L.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T04%3A31%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=IbERF71,%20with%20IbMYB340%20and%20IbbHLH2,%20coregulates%20anthocyanin%20accumulation%20by%20binding%20to%20the%20IbANS1%20promoter%20in%20purple-fleshed%20sweet%20potato%20(Ipomoea%20batatas%20L.)&rft.jtitle=Plant%20cell%20reports&rft.au=Ning,%20Zhiyuan&rft.date=2021&rft.volume=40&rft.issue=1&rft.spage=157&rft.epage=169&rft.pages=157-169&rft.issn=0721-7714&rft.eissn=1432-203X&rft_id=info:doi/10.1007/s00299-020-02621-0&rft_dat=%3Cproquest_cross%3E2478285759%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2478285759&rft_id=info:pmid/33084965&rfr_iscdi=true |