Green synthesis of silver nanoparticles using canthaxanthin from Dietzia maris AURCCBT01 and their cytotoxic properties against human keratinocyte cell line
Aim Nano‐biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study. Methods and Results The pigment extra...
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| Veröffentlicht in: | Journal of applied microbiology 2021-05, Vol.130 (5), p.1730-1744 |
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| container_title | Journal of applied microbiology |
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| creator | Venil, C.K. Malathi, M. Velmurugan, P. Renuka Devi, P. |
| description | Aim
Nano‐biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study.
Methods and Results
The pigment extracted from D. maris AURCCBT01 was identified as canthaxanthin using UV‐VIS spectroscopy, FTIR, NMR (1H NMR and 13C NMR) and MS. Canthaxanthin, treated with silver nitrate solution, produced canthaxanthin‐mediated silver nanoparticles and they were characterized by UV‐VIS spectroscopy, FTIR, XRD, FESEM‐EDX and TEM‐SAED techniques. UV‐VIS spectroscopy pointed out an absorption band at 420 nm, relating to the surface plasmon resonance of silver nanoparticles. FTIR findings suggested that the diverse functional groups of canthaxanthin bio‐molecules played a significant task in capping the silver nanoparticles. XRD analysis exhibited 40·20 nm for the crystal size of nanoparticles. FESEM and TEM exhibited that the biosynthesized silver nanoparticles were spherical in shape with crystalline nature and the particle size was 40–50 nm. Moreover, the cytotoxicity assessment of the synthesized nanoparticles in HaCaT revealed significant cytotoxicity in the cultured cells with an IC50 value of 43 µg ml−1.
Conclusion
Stable silver nanoparticles synthesized using canthaxanthin from D. maris AURCCBT01 were found effective for application in wound healing activity.
Significance and Impact of the Study
Biosynthesized silver nanoparticles via canthaxanthin bacterial pigment exhibited their cytotoxicity effect in HaCaT and testified their eventual therapeutic potential in the wound healing activity with no side effects in a cost effective and eco‐friendly process. |
| doi_str_mv | 10.1111/jam.14889 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2452508312</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2511103212</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-9acee69c83fafbf70640a41f7cceba5a6af333204d527d472ce1fe02490d550a3</originalsourceid><addsrcrecordid>eNp1kctuEzEUhq2qiJbCghdAlropi2l9Gc9lmQYooCIk1K6tE89x63TGTu0ZaHgWHhYnaVkg1Qvbi-98-nV-Qt5ydsrzOVvCcMrLpmn3yCGXlSpEVYv97b8sFKvFAXmV0pIxLpmqXpIDKVndKMkOyZ-LiOhpWvvxFpNLNFiaXP8TI_Xgwwri6EyPiU7J-RtqIHPwsLmdpzaGgX5wOP52QAeIeXx2_WM-P79inILvaHa6SM16DGN4cIauYlhhNmYf3IDzaaS30wCe3mGE0fmQUaQG-572zuNr8sJCn_DN43tErj99vJp_Li6_X3yZzy4LI5VsixYMYtWaRlqwC1uzqmRQclsbgwtQUIGVUgpWdkrUXVkLg9wiE2XLOqUYyCNysvPmfPcTplEPLm1SgMcwJS1KJRRrJBcZPf4PXYYp-pxOC5W7YFJsqfc7ysSQUkSrV9HlBa01Z3pTmc6V6W1lmX33aJwWA3b_yKeOMnC2A365HtfPm_TX2bed8i9iI6Lx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2511103212</pqid></control><display><type>article</type><title>Green synthesis of silver nanoparticles using canthaxanthin from Dietzia maris AURCCBT01 and their cytotoxic properties against human keratinocyte cell line</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Venil, C.K. ; Malathi, M. ; Velmurugan, P. ; Renuka Devi, P.</creator><creatorcontrib>Venil, C.K. ; Malathi, M. ; Velmurugan, P. ; Renuka Devi, P.</creatorcontrib><description>Aim
Nano‐biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study.
Methods and Results
The pigment extracted from D. maris AURCCBT01 was identified as canthaxanthin using UV‐VIS spectroscopy, FTIR, NMR (1H NMR and 13C NMR) and MS. Canthaxanthin, treated with silver nitrate solution, produced canthaxanthin‐mediated silver nanoparticles and they were characterized by UV‐VIS spectroscopy, FTIR, XRD, FESEM‐EDX and TEM‐SAED techniques. UV‐VIS spectroscopy pointed out an absorption band at 420 nm, relating to the surface plasmon resonance of silver nanoparticles. FTIR findings suggested that the diverse functional groups of canthaxanthin bio‐molecules played a significant task in capping the silver nanoparticles. XRD analysis exhibited 40·20 nm for the crystal size of nanoparticles. FESEM and TEM exhibited that the biosynthesized silver nanoparticles were spherical in shape with crystalline nature and the particle size was 40–50 nm. Moreover, the cytotoxicity assessment of the synthesized nanoparticles in HaCaT revealed significant cytotoxicity in the cultured cells with an IC50 value of 43 µg ml−1.
Conclusion
Stable silver nanoparticles synthesized using canthaxanthin from D. maris AURCCBT01 were found effective for application in wound healing activity.
Significance and Impact of the Study
Biosynthesized silver nanoparticles via canthaxanthin bacterial pigment exhibited their cytotoxicity effect in HaCaT and testified their eventual therapeutic potential in the wound healing activity with no side effects in a cost effective and eco‐friendly process.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14889</identifier><identifier>PMID: 33078530</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Absorption spectra ; Actinobacteria - metabolism ; Biotechnology ; canthaxanthin ; Canthaxanthin - isolation & purification ; Cell Line ; characterization ; Cytotoxicity ; Dietzia ; Dietzia maris ; Fourier transforms ; Functional groups ; Gold ; Green Chemistry Technology ; green synthesis ; HaCaT Cells ; human keratinocyte cell line ; Humans ; Infrared spectroscopy ; Inhibitory Concentration 50 ; Keratinocytes - drug effects ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - toxicity ; Nanoparticles ; Nanotechnology ; NMR ; Nuclear magnetic resonance ; Particle Size ; Side effects ; Silver ; Silver nitrate ; Spectroscopy ; Spectroscopy, Fourier Transform Infrared ; Spectrum analysis ; Surface plasmon resonance ; Synthesis ; Toxicity ; Wound Healing</subject><ispartof>Journal of applied microbiology, 2021-05, Vol.130 (5), p.1730-1744</ispartof><rights>2020 The Society for Applied Microbiology</rights><rights>2020 The Society for Applied Microbiology.</rights><rights>Copyright © 2021 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-9acee69c83fafbf70640a41f7cceba5a6af333204d527d472ce1fe02490d550a3</citedby><cites>FETCH-LOGICAL-c3539-9acee69c83fafbf70640a41f7cceba5a6af333204d527d472ce1fe02490d550a3</cites><orcidid>0000-0002-1810-9480</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14889$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14889$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33078530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Venil, C.K.</creatorcontrib><creatorcontrib>Malathi, M.</creatorcontrib><creatorcontrib>Velmurugan, P.</creatorcontrib><creatorcontrib>Renuka Devi, P.</creatorcontrib><title>Green synthesis of silver nanoparticles using canthaxanthin from Dietzia maris AURCCBT01 and their cytotoxic properties against human keratinocyte cell line</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
Nano‐biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study.
Methods and Results
The pigment extracted from D. maris AURCCBT01 was identified as canthaxanthin using UV‐VIS spectroscopy, FTIR, NMR (1H NMR and 13C NMR) and MS. Canthaxanthin, treated with silver nitrate solution, produced canthaxanthin‐mediated silver nanoparticles and they were characterized by UV‐VIS spectroscopy, FTIR, XRD, FESEM‐EDX and TEM‐SAED techniques. UV‐VIS spectroscopy pointed out an absorption band at 420 nm, relating to the surface plasmon resonance of silver nanoparticles. FTIR findings suggested that the diverse functional groups of canthaxanthin bio‐molecules played a significant task in capping the silver nanoparticles. XRD analysis exhibited 40·20 nm for the crystal size of nanoparticles. FESEM and TEM exhibited that the biosynthesized silver nanoparticles were spherical in shape with crystalline nature and the particle size was 40–50 nm. Moreover, the cytotoxicity assessment of the synthesized nanoparticles in HaCaT revealed significant cytotoxicity in the cultured cells with an IC50 value of 43 µg ml−1.
Conclusion
Stable silver nanoparticles synthesized using canthaxanthin from D. maris AURCCBT01 were found effective for application in wound healing activity.
Significance and Impact of the Study
Biosynthesized silver nanoparticles via canthaxanthin bacterial pigment exhibited their cytotoxicity effect in HaCaT and testified their eventual therapeutic potential in the wound healing activity with no side effects in a cost effective and eco‐friendly process.</description><subject>Absorption spectra</subject><subject>Actinobacteria - metabolism</subject><subject>Biotechnology</subject><subject>canthaxanthin</subject><subject>Canthaxanthin - isolation & purification</subject><subject>Cell Line</subject><subject>characterization</subject><subject>Cytotoxicity</subject><subject>Dietzia</subject><subject>Dietzia maris</subject><subject>Fourier transforms</subject><subject>Functional groups</subject><subject>Gold</subject><subject>Green Chemistry Technology</subject><subject>green synthesis</subject><subject>HaCaT Cells</subject><subject>human keratinocyte cell line</subject><subject>Humans</subject><subject>Infrared spectroscopy</subject><subject>Inhibitory Concentration 50</subject><subject>Keratinocytes - drug effects</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - toxicity</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Particle Size</subject><subject>Side effects</subject><subject>Silver</subject><subject>Silver nitrate</subject><subject>Spectroscopy</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Spectrum analysis</subject><subject>Surface plasmon resonance</subject><subject>Synthesis</subject><subject>Toxicity</subject><subject>Wound Healing</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctuEzEUhq2qiJbCghdAlropi2l9Gc9lmQYooCIk1K6tE89x63TGTu0ZaHgWHhYnaVkg1Qvbi-98-nV-Qt5ydsrzOVvCcMrLpmn3yCGXlSpEVYv97b8sFKvFAXmV0pIxLpmqXpIDKVndKMkOyZ-LiOhpWvvxFpNLNFiaXP8TI_Xgwwri6EyPiU7J-RtqIHPwsLmdpzaGgX5wOP52QAeIeXx2_WM-P79inILvaHa6SM16DGN4cIauYlhhNmYf3IDzaaS30wCe3mGE0fmQUaQG-572zuNr8sJCn_DN43tErj99vJp_Li6_X3yZzy4LI5VsixYMYtWaRlqwC1uzqmRQclsbgwtQUIGVUgpWdkrUXVkLg9wiE2XLOqUYyCNysvPmfPcTplEPLm1SgMcwJS1KJRRrJBcZPf4PXYYp-pxOC5W7YFJsqfc7ysSQUkSrV9HlBa01Z3pTmc6V6W1lmX33aJwWA3b_yKeOMnC2A365HtfPm_TX2bed8i9iI6Lx</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Venil, C.K.</creator><creator>Malathi, M.</creator><creator>Velmurugan, P.</creator><creator>Renuka Devi, P.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1810-9480</orcidid></search><sort><creationdate>202105</creationdate><title>Green synthesis of silver nanoparticles using canthaxanthin from Dietzia maris AURCCBT01 and their cytotoxic properties against human keratinocyte cell line</title><author>Venil, C.K. ; Malathi, M. ; Velmurugan, P. ; Renuka Devi, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-9acee69c83fafbf70640a41f7cceba5a6af333204d527d472ce1fe02490d550a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Absorption spectra</topic><topic>Actinobacteria - metabolism</topic><topic>Biotechnology</topic><topic>canthaxanthin</topic><topic>Canthaxanthin - isolation & purification</topic><topic>Cell Line</topic><topic>characterization</topic><topic>Cytotoxicity</topic><topic>Dietzia</topic><topic>Dietzia maris</topic><topic>Fourier transforms</topic><topic>Functional groups</topic><topic>Gold</topic><topic>Green Chemistry Technology</topic><topic>green synthesis</topic><topic>HaCaT Cells</topic><topic>human keratinocyte cell line</topic><topic>Humans</topic><topic>Infrared spectroscopy</topic><topic>Inhibitory Concentration 50</topic><topic>Keratinocytes - drug effects</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - toxicity</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Particle Size</topic><topic>Side effects</topic><topic>Silver</topic><topic>Silver nitrate</topic><topic>Spectroscopy</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Spectrum analysis</topic><topic>Surface plasmon resonance</topic><topic>Synthesis</topic><topic>Toxicity</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Venil, C.K.</creatorcontrib><creatorcontrib>Malathi, M.</creatorcontrib><creatorcontrib>Velmurugan, P.</creatorcontrib><creatorcontrib>Renuka Devi, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Venil, C.K.</au><au>Malathi, M.</au><au>Velmurugan, P.</au><au>Renuka Devi, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green synthesis of silver nanoparticles using canthaxanthin from Dietzia maris AURCCBT01 and their cytotoxic properties against human keratinocyte cell line</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2021-05</date><risdate>2021</risdate><volume>130</volume><issue>5</issue><spage>1730</spage><epage>1744</epage><pages>1730-1744</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
Nano‐biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study.
Methods and Results
The pigment extracted from D. maris AURCCBT01 was identified as canthaxanthin using UV‐VIS spectroscopy, FTIR, NMR (1H NMR and 13C NMR) and MS. Canthaxanthin, treated with silver nitrate solution, produced canthaxanthin‐mediated silver nanoparticles and they were characterized by UV‐VIS spectroscopy, FTIR, XRD, FESEM‐EDX and TEM‐SAED techniques. UV‐VIS spectroscopy pointed out an absorption band at 420 nm, relating to the surface plasmon resonance of silver nanoparticles. FTIR findings suggested that the diverse functional groups of canthaxanthin bio‐molecules played a significant task in capping the silver nanoparticles. XRD analysis exhibited 40·20 nm for the crystal size of nanoparticles. FESEM and TEM exhibited that the biosynthesized silver nanoparticles were spherical in shape with crystalline nature and the particle size was 40–50 nm. Moreover, the cytotoxicity assessment of the synthesized nanoparticles in HaCaT revealed significant cytotoxicity in the cultured cells with an IC50 value of 43 µg ml−1.
Conclusion
Stable silver nanoparticles synthesized using canthaxanthin from D. maris AURCCBT01 were found effective for application in wound healing activity.
Significance and Impact of the Study
Biosynthesized silver nanoparticles via canthaxanthin bacterial pigment exhibited their cytotoxicity effect in HaCaT and testified their eventual therapeutic potential in the wound healing activity with no side effects in a cost effective and eco‐friendly process.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33078530</pmid><doi>10.1111/jam.14889</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-1810-9480</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Absorption spectra Actinobacteria - metabolism Biotechnology canthaxanthin Canthaxanthin - isolation & purification Cell Line characterization Cytotoxicity Dietzia Dietzia maris Fourier transforms Functional groups Gold Green Chemistry Technology green synthesis HaCaT Cells human keratinocyte cell line Humans Infrared spectroscopy Inhibitory Concentration 50 Keratinocytes - drug effects Metal Nanoparticles - chemistry Metal Nanoparticles - toxicity Nanoparticles Nanotechnology NMR Nuclear magnetic resonance Particle Size Side effects Silver Silver nitrate Spectroscopy Spectroscopy, Fourier Transform Infrared Spectrum analysis Surface plasmon resonance Synthesis Toxicity Wound Healing |
| title | Green synthesis of silver nanoparticles using canthaxanthin from Dietzia maris AURCCBT01 and their cytotoxic properties against human keratinocyte cell line |
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