Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system
Collecting biofilm samples from drinking water distribution systems (DWDSs) is challenging due to limited access to the pipes during regular operations. We report here the analysis of microbial communities in biofilm and water samples collected from sensors installed in a DWDS where monochloramine i...
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Veröffentlicht in: | The Science of the total environment 2021-04, Vol.766, p.142314-142314, Article 142314 |
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description | Collecting biofilm samples from drinking water distribution systems (DWDSs) is challenging due to limited access to the pipes during regular operations. We report here the analysis of microbial communities in biofilm and water samples collected from sensors installed in a DWDS where monochloramine is used as a residual disinfectant. A total of 52 biofilm samples and 14 bulk water samples were collected from 17 pipe sections representing different water ages. Prokaryotic genome copies (bacterial and archaeal 16S rRNA genes, Mycobacterium spp., ammonia-oxidizing bacteria (AOB), and cyanobacteria) were quantified with droplet digital PCR, which revealed the abundance of these genes in both biofilm and water samples. Prokaryotic 16S rRNA gene sequencing analysis was carried out for a subset of the samples (12 samples from four sites). Mycobacterium and AOB species were dominant in the DWDS sections with low water age and sufficient residual monochloramine, whereas Nitrospira species (nitrite-oxidizing bacteria) dominated in the sections with higher water age and depleted monochloramine level, suggesting the occurrence of nitrification in the studied DWDS. The present study provides novel information on the abundance and identity of prokaryotes in biofilms and water in a full-scale operational DWDS.
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•Mycobacterium and AOB were dominant in WDS sections with low water age.•Nitrospira predominated in the WDS sections with higher water age.•Results suggested the occurrence of nitrification in the studied WDS.•Microbial community structure was primarily affected by difference in WDS sections. |
doi_str_mv | 10.1016/j.scitotenv.2020.142314 |
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[Display omitted]
•Mycobacterium and AOB were dominant in WDS sections with low water age.•Nitrospira predominated in the WDS sections with higher water age.•Results suggested the occurrence of nitrification in the studied WDS.•Microbial community structure was primarily affected by difference in WDS sections.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2020.142314</identifier><identifier>PMID: 33077212</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>16S rRNA gene sequencing ; Biofilm ; Biofilms ; Drinking Water ; Nitrification ; RNA, Ribosomal, 16S - genetics ; Water distribution system ; Water Microbiology ; Water quality ; Water Supply</subject><ispartof>The Science of the total environment, 2021-04, Vol.766, p.142314-142314, Article 142314</ispartof><rights>2020</rights><rights>Copyright © 2020. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-6b6ba13b3ed441ae1fba9207b9c1b8a8bb852d7f9d9f63ad96a7fd80eed333ee3</citedby><cites>FETCH-LOGICAL-c464t-6b6ba13b3ed441ae1fba9207b9c1b8a8bb852d7f9d9f63ad96a7fd80eed333ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.scitotenv.2020.142314$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33077212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitajima, Masaaki</creatorcontrib><creatorcontrib>Cruz, Mercedes C.</creatorcontrib><creatorcontrib>Williams, Rohan B.H.</creatorcontrib><creatorcontrib>Wuertz, Stefan</creatorcontrib><creatorcontrib>Whittle, Andrew J.</creatorcontrib><title>Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Collecting biofilm samples from drinking water distribution systems (DWDSs) is challenging due to limited access to the pipes during regular operations. We report here the analysis of microbial communities in biofilm and water samples collected from sensors installed in a DWDS where monochloramine is used as a residual disinfectant. A total of 52 biofilm samples and 14 bulk water samples were collected from 17 pipe sections representing different water ages. Prokaryotic genome copies (bacterial and archaeal 16S rRNA genes, Mycobacterium spp., ammonia-oxidizing bacteria (AOB), and cyanobacteria) were quantified with droplet digital PCR, which revealed the abundance of these genes in both biofilm and water samples. Prokaryotic 16S rRNA gene sequencing analysis was carried out for a subset of the samples (12 samples from four sites). Mycobacterium and AOB species were dominant in the DWDS sections with low water age and sufficient residual monochloramine, whereas Nitrospira species (nitrite-oxidizing bacteria) dominated in the sections with higher water age and depleted monochloramine level, suggesting the occurrence of nitrification in the studied DWDS. The present study provides novel information on the abundance and identity of prokaryotes in biofilms and water in a full-scale operational DWDS.
[Display omitted]
•Mycobacterium and AOB were dominant in WDS sections with low water age.•Nitrospira predominated in the WDS sections with higher water age.•Results suggested the occurrence of nitrification in the studied WDS.•Microbial community structure was primarily affected by difference in WDS sections.</description><subject>16S rRNA gene sequencing</subject><subject>Biofilm</subject><subject>Biofilms</subject><subject>Drinking Water</subject><subject>Nitrification</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Water distribution system</subject><subject>Water Microbiology</subject><subject>Water quality</subject><subject>Water Supply</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v1DAQxS0EotvCVwAfuWTxv43jY1VRQCriAmfLjidolsQOtlO0356kW3rtXDxjv_dG_hHynrM9Z7z9eNyXHmuqEO_3gon1VgnJ1Quy4502DWeifUl2jKmuMa3RF-SylCNbS3f8NbmQkmktuNiR-g37nDy6kTq_xOBiD9TFQPs0TUvEetq6ORWsmCLFSD2mAcep0IexmXEGWiCWlMv27GjIGH9j_EX_ugqZBiw1o18e_OVUKkxvyKvBjQXePp5X5Oftpx83X5q775-_3lzfNb1qVW1a33rHpZcQlOIO-OCdEUx703Pfuc777iCCHkwwQytdMK3TQ-gYQJBSAsgr8uGcO-f0Z4FS7YSlh3F0EdJSrFAHcWD8oMwq1WfpSqOUDIOdM04unyxndkNuj_YJud2Q2zPy1fnuccniJwhPvv-MV8H1WQDrV-8R8hYEK-eAGfpqQ8Jnl_wDF4-ajg</recordid><startdate>20210420</startdate><enddate>20210420</enddate><creator>Kitajima, Masaaki</creator><creator>Cruz, Mercedes C.</creator><creator>Williams, Rohan B.H.</creator><creator>Wuertz, Stefan</creator><creator>Whittle, Andrew J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210420</creationdate><title>Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system</title><author>Kitajima, Masaaki ; Cruz, Mercedes C. ; Williams, Rohan B.H. ; Wuertz, Stefan ; Whittle, Andrew J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-6b6ba13b3ed441ae1fba9207b9c1b8a8bb852d7f9d9f63ad96a7fd80eed333ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>16S rRNA gene sequencing</topic><topic>Biofilm</topic><topic>Biofilms</topic><topic>Drinking Water</topic><topic>Nitrification</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Water distribution system</topic><topic>Water Microbiology</topic><topic>Water quality</topic><topic>Water Supply</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitajima, Masaaki</creatorcontrib><creatorcontrib>Cruz, Mercedes C.</creatorcontrib><creatorcontrib>Williams, Rohan B.H.</creatorcontrib><creatorcontrib>Wuertz, Stefan</creatorcontrib><creatorcontrib>Whittle, Andrew J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitajima, Masaaki</au><au>Cruz, Mercedes C.</au><au>Williams, Rohan B.H.</au><au>Wuertz, Stefan</au><au>Whittle, Andrew J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2021-04-20</date><risdate>2021</risdate><volume>766</volume><spage>142314</spage><epage>142314</epage><pages>142314-142314</pages><artnum>142314</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>Collecting biofilm samples from drinking water distribution systems (DWDSs) is challenging due to limited access to the pipes during regular operations. We report here the analysis of microbial communities in biofilm and water samples collected from sensors installed in a DWDS where monochloramine is used as a residual disinfectant. A total of 52 biofilm samples and 14 bulk water samples were collected from 17 pipe sections representing different water ages. Prokaryotic genome copies (bacterial and archaeal 16S rRNA genes, Mycobacterium spp., ammonia-oxidizing bacteria (AOB), and cyanobacteria) were quantified with droplet digital PCR, which revealed the abundance of these genes in both biofilm and water samples. Prokaryotic 16S rRNA gene sequencing analysis was carried out for a subset of the samples (12 samples from four sites). Mycobacterium and AOB species were dominant in the DWDS sections with low water age and sufficient residual monochloramine, whereas Nitrospira species (nitrite-oxidizing bacteria) dominated in the sections with higher water age and depleted monochloramine level, suggesting the occurrence of nitrification in the studied DWDS. The present study provides novel information on the abundance and identity of prokaryotes in biofilms and water in a full-scale operational DWDS.
[Display omitted]
•Mycobacterium and AOB were dominant in WDS sections with low water age.•Nitrospira predominated in the WDS sections with higher water age.•Results suggested the occurrence of nitrification in the studied WDS.•Microbial community structure was primarily affected by difference in WDS sections.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>33077212</pmid><doi>10.1016/j.scitotenv.2020.142314</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 16S rRNA gene sequencing Biofilm Biofilms Drinking Water Nitrification RNA, Ribosomal, 16S - genetics Water distribution system Water Microbiology Water quality Water Supply |
title | Microbial abundance and community composition in biofilms on in-pipe sensors in a drinking water distribution system |
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