Occurrence and molecular identification of zoonotic microsporidia in pet budgerigars (Melopsittacus undulatus) in Turkey
Abstract Encephalitozoon spp. and Enterocytozoon bieneusi are well-known microsporidian pathogens, recently classified as fungi, infecting humans and reptiles, mammals, and birds. Budgerigars (Melopsittacus undulates) are the most preferred captive pet birds in the households. Prevalence and molecul...
Gespeichert in:
| Veröffentlicht in: | Medical mycology (Oxford) 2020-10, Vol.59 (6), p.585-591 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 591 |
|---|---|
| container_issue | 6 |
| container_start_page | 585 |
| container_title | Medical mycology (Oxford) |
| container_volume | 59 |
| creator | Pekmezci, Didem Yetismis, Gamze Esin, Cagatay Duzlu, Onder Colak, Zeynep Nurselin Inci, Abdullah Pekmezci, Gokmen Zafer Yildirim, Alparslan |
| description | Abstract
Encephalitozoon spp. and Enterocytozoon bieneusi are well-known microsporidian pathogens, recently classified as fungi, infecting humans and reptiles, mammals, and birds. Budgerigars (Melopsittacus undulates) are the most preferred captive pet birds in the households. Prevalence and molecular data on microsporidian species in budgerigars are scarce worldwide. The aim of the present study was to investigate the occurrence and genotypes of Encephalitozoon spp. and E. bieneusi in budgerigars, and to reveal their zoonotic potential. A total of 143 fecal samples were collected from owned healthy budgerigars in Turkey. Encephalitozoon spp. and E. bieneusi were examined by nested PCR targeting the ribosomal internal transcribed spacer (ITS) region and sequenced for identifying Encephalitozoon spp. and E. bieneusi. The overall prevalence of E. hellem and E. bieneusi was 14.7% (21/143) and 3.5% (5/143), respectively. Two genotypes of E. hellem were identified, including one known 1A (n = 18) and a novel TURK1B (n = 3). In addition, we determined two E. bieneusi genotypes, including one known N (n = 2) and a novel TURKM1 (n = 3). E. hellem 1A and novel TURK1B clustered as a sister taxon, and genotype N and novel TURKM1 genotypes fall into group 2 of E. bieneusi in the phylogenetic tree. Novel genotypes of E. hellem and E. bieneusi were described for the first time in the avian host. Moreover, E. bieneusi genotype N was first detected in avian hosts in the present study. This study contributes to the current knowledge on the molecular epidemiology and transmission dynamics of E. hellem and E. bieneusi.
Lay Summary
Spore producing microsporidia are ubiquitous, obligate, and intracellular fungus defined as emerging opportunistic pathogens of humans, livestock, companion animals, wild mammals, birds, and water worldwide. The occurrence of microsporidia in animals could be risky for human public health. |
| doi_str_mv | 10.1093/mmy/myaa088 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2452099777</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/mmy/myaa088</oup_id><sourcerecordid>2452099777</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-2ff811f49ee038403512261236adf4a9d3529045ea99e5db37128c465fe159a3</originalsourceid><addsrcrecordid>eNp9kDtLBDEURoMovit7SSUrMm4e80opiy9Qttl-yCY3Ep2ZjHmA4693ll0tre4tDge-g9AFJbeUCD7vunHejVKSut5DxzQvScYqIvann5ci41VdHqGTEN4JoZVg_BAdcU4qQmtxjL6WSiXvoVeAZa9x51pQqZUeWw19tMYqGa3rsTP427neRatwZ5V3YXDeaiux7fEAEa-TfgNv36QPePYKrRuCjVGqFHDq9aSMKVxv4FXyHzCeoQMj2wDnu3uKVg_3q8VT9rJ8fF7cvWSK0zJmzJiaUpMLAMLrnPCCMlZSxkupTS6F5gUTJC9ACgGFXvOKslrlZWGAFkLyUzTbagfvPhOE2HQ2KGhb2YNLoWF5wYgQVVVN6M0W3YwLHkwzeNtJPzaUNJvSzVS62ZWe6MudOK070H_sb9oJuNoCLg3_mn4AslCKGw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2452099777</pqid></control><display><type>article</type><title>Occurrence and molecular identification of zoonotic microsporidia in pet budgerigars (Melopsittacus undulatus) in Turkey</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Pekmezci, Didem ; Yetismis, Gamze ; Esin, Cagatay ; Duzlu, Onder ; Colak, Zeynep Nurselin ; Inci, Abdullah ; Pekmezci, Gokmen Zafer ; Yildirim, Alparslan</creator><creatorcontrib>Pekmezci, Didem ; Yetismis, Gamze ; Esin, Cagatay ; Duzlu, Onder ; Colak, Zeynep Nurselin ; Inci, Abdullah ; Pekmezci, Gokmen Zafer ; Yildirim, Alparslan</creatorcontrib><description>Abstract
Encephalitozoon spp. and Enterocytozoon bieneusi are well-known microsporidian pathogens, recently classified as fungi, infecting humans and reptiles, mammals, and birds. Budgerigars (Melopsittacus undulates) are the most preferred captive pet birds in the households. Prevalence and molecular data on microsporidian species in budgerigars are scarce worldwide. The aim of the present study was to investigate the occurrence and genotypes of Encephalitozoon spp. and E. bieneusi in budgerigars, and to reveal their zoonotic potential. A total of 143 fecal samples were collected from owned healthy budgerigars in Turkey. Encephalitozoon spp. and E. bieneusi were examined by nested PCR targeting the ribosomal internal transcribed spacer (ITS) region and sequenced for identifying Encephalitozoon spp. and E. bieneusi. The overall prevalence of E. hellem and E. bieneusi was 14.7% (21/143) and 3.5% (5/143), respectively. Two genotypes of E. hellem were identified, including one known 1A (n = 18) and a novel TURK1B (n = 3). In addition, we determined two E. bieneusi genotypes, including one known N (n = 2) and a novel TURKM1 (n = 3). E. hellem 1A and novel TURK1B clustered as a sister taxon, and genotype N and novel TURKM1 genotypes fall into group 2 of E. bieneusi in the phylogenetic tree. Novel genotypes of E. hellem and E. bieneusi were described for the first time in the avian host. Moreover, E. bieneusi genotype N was first detected in avian hosts in the present study. This study contributes to the current knowledge on the molecular epidemiology and transmission dynamics of E. hellem and E. bieneusi.
Lay Summary
Spore producing microsporidia are ubiquitous, obligate, and intracellular fungus defined as emerging opportunistic pathogens of humans, livestock, companion animals, wild mammals, birds, and water worldwide. The occurrence of microsporidia in animals could be risky for human public health.</description><identifier>ISSN: 1369-3786</identifier><identifier>EISSN: 1460-2709</identifier><identifier>DOI: 10.1093/mmy/myaa088</identifier><identifier>PMID: 33070189</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><ispartof>Medical mycology (Oxford), 2020-10, Vol.59 (6), p.585-591</ispartof><rights>The Author(s) 2020. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-2ff811f49ee038403512261236adf4a9d3529045ea99e5db37128c465fe159a3</citedby><cites>FETCH-LOGICAL-c316t-2ff811f49ee038403512261236adf4a9d3529045ea99e5db37128c465fe159a3</cites><orcidid>0000-0003-2072-8165</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33070189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pekmezci, Didem</creatorcontrib><creatorcontrib>Yetismis, Gamze</creatorcontrib><creatorcontrib>Esin, Cagatay</creatorcontrib><creatorcontrib>Duzlu, Onder</creatorcontrib><creatorcontrib>Colak, Zeynep Nurselin</creatorcontrib><creatorcontrib>Inci, Abdullah</creatorcontrib><creatorcontrib>Pekmezci, Gokmen Zafer</creatorcontrib><creatorcontrib>Yildirim, Alparslan</creatorcontrib><title>Occurrence and molecular identification of zoonotic microsporidia in pet budgerigars (Melopsittacus undulatus) in Turkey</title><title>Medical mycology (Oxford)</title><addtitle>Med Mycol</addtitle><description>Abstract
Encephalitozoon spp. and Enterocytozoon bieneusi are well-known microsporidian pathogens, recently classified as fungi, infecting humans and reptiles, mammals, and birds. Budgerigars (Melopsittacus undulates) are the most preferred captive pet birds in the households. Prevalence and molecular data on microsporidian species in budgerigars are scarce worldwide. The aim of the present study was to investigate the occurrence and genotypes of Encephalitozoon spp. and E. bieneusi in budgerigars, and to reveal their zoonotic potential. A total of 143 fecal samples were collected from owned healthy budgerigars in Turkey. Encephalitozoon spp. and E. bieneusi were examined by nested PCR targeting the ribosomal internal transcribed spacer (ITS) region and sequenced for identifying Encephalitozoon spp. and E. bieneusi. The overall prevalence of E. hellem and E. bieneusi was 14.7% (21/143) and 3.5% (5/143), respectively. Two genotypes of E. hellem were identified, including one known 1A (n = 18) and a novel TURK1B (n = 3). In addition, we determined two E. bieneusi genotypes, including one known N (n = 2) and a novel TURKM1 (n = 3). E. hellem 1A and novel TURK1B clustered as a sister taxon, and genotype N and novel TURKM1 genotypes fall into group 2 of E. bieneusi in the phylogenetic tree. Novel genotypes of E. hellem and E. bieneusi were described for the first time in the avian host. Moreover, E. bieneusi genotype N was first detected in avian hosts in the present study. This study contributes to the current knowledge on the molecular epidemiology and transmission dynamics of E. hellem and E. bieneusi.
Lay Summary
Spore producing microsporidia are ubiquitous, obligate, and intracellular fungus defined as emerging opportunistic pathogens of humans, livestock, companion animals, wild mammals, birds, and water worldwide. The occurrence of microsporidia in animals could be risky for human public health.</description><issn>1369-3786</issn><issn>1460-2709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kDtLBDEURoMovit7SSUrMm4e80opiy9Qttl-yCY3Ep2ZjHmA4693ll0tre4tDge-g9AFJbeUCD7vunHejVKSut5DxzQvScYqIvann5ci41VdHqGTEN4JoZVg_BAdcU4qQmtxjL6WSiXvoVeAZa9x51pQqZUeWw19tMYqGa3rsTP427neRatwZ5V3YXDeaiux7fEAEa-TfgNv36QPePYKrRuCjVGqFHDq9aSMKVxv4FXyHzCeoQMj2wDnu3uKVg_3q8VT9rJ8fF7cvWSK0zJmzJiaUpMLAMLrnPCCMlZSxkupTS6F5gUTJC9ACgGFXvOKslrlZWGAFkLyUzTbagfvPhOE2HQ2KGhb2YNLoWF5wYgQVVVN6M0W3YwLHkwzeNtJPzaUNJvSzVS62ZWe6MudOK070H_sb9oJuNoCLg3_mn4AslCKGw</recordid><startdate>20201017</startdate><enddate>20201017</enddate><creator>Pekmezci, Didem</creator><creator>Yetismis, Gamze</creator><creator>Esin, Cagatay</creator><creator>Duzlu, Onder</creator><creator>Colak, Zeynep Nurselin</creator><creator>Inci, Abdullah</creator><creator>Pekmezci, Gokmen Zafer</creator><creator>Yildirim, Alparslan</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2072-8165</orcidid></search><sort><creationdate>20201017</creationdate><title>Occurrence and molecular identification of zoonotic microsporidia in pet budgerigars (Melopsittacus undulatus) in Turkey</title><author>Pekmezci, Didem ; Yetismis, Gamze ; Esin, Cagatay ; Duzlu, Onder ; Colak, Zeynep Nurselin ; Inci, Abdullah ; Pekmezci, Gokmen Zafer ; Yildirim, Alparslan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-2ff811f49ee038403512261236adf4a9d3529045ea99e5db37128c465fe159a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pekmezci, Didem</creatorcontrib><creatorcontrib>Yetismis, Gamze</creatorcontrib><creatorcontrib>Esin, Cagatay</creatorcontrib><creatorcontrib>Duzlu, Onder</creatorcontrib><creatorcontrib>Colak, Zeynep Nurselin</creatorcontrib><creatorcontrib>Inci, Abdullah</creatorcontrib><creatorcontrib>Pekmezci, Gokmen Zafer</creatorcontrib><creatorcontrib>Yildirim, Alparslan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Medical mycology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pekmezci, Didem</au><au>Yetismis, Gamze</au><au>Esin, Cagatay</au><au>Duzlu, Onder</au><au>Colak, Zeynep Nurselin</au><au>Inci, Abdullah</au><au>Pekmezci, Gokmen Zafer</au><au>Yildirim, Alparslan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence and molecular identification of zoonotic microsporidia in pet budgerigars (Melopsittacus undulatus) in Turkey</atitle><jtitle>Medical mycology (Oxford)</jtitle><addtitle>Med Mycol</addtitle><date>2020-10-17</date><risdate>2020</risdate><volume>59</volume><issue>6</issue><spage>585</spage><epage>591</epage><pages>585-591</pages><issn>1369-3786</issn><eissn>1460-2709</eissn><abstract>Abstract
Encephalitozoon spp. and Enterocytozoon bieneusi are well-known microsporidian pathogens, recently classified as fungi, infecting humans and reptiles, mammals, and birds. Budgerigars (Melopsittacus undulates) are the most preferred captive pet birds in the households. Prevalence and molecular data on microsporidian species in budgerigars are scarce worldwide. The aim of the present study was to investigate the occurrence and genotypes of Encephalitozoon spp. and E. bieneusi in budgerigars, and to reveal their zoonotic potential. A total of 143 fecal samples were collected from owned healthy budgerigars in Turkey. Encephalitozoon spp. and E. bieneusi were examined by nested PCR targeting the ribosomal internal transcribed spacer (ITS) region and sequenced for identifying Encephalitozoon spp. and E. bieneusi. The overall prevalence of E. hellem and E. bieneusi was 14.7% (21/143) and 3.5% (5/143), respectively. Two genotypes of E. hellem were identified, including one known 1A (n = 18) and a novel TURK1B (n = 3). In addition, we determined two E. bieneusi genotypes, including one known N (n = 2) and a novel TURKM1 (n = 3). E. hellem 1A and novel TURK1B clustered as a sister taxon, and genotype N and novel TURKM1 genotypes fall into group 2 of E. bieneusi in the phylogenetic tree. Novel genotypes of E. hellem and E. bieneusi were described for the first time in the avian host. Moreover, E. bieneusi genotype N was first detected in avian hosts in the present study. This study contributes to the current knowledge on the molecular epidemiology and transmission dynamics of E. hellem and E. bieneusi.
Lay Summary
Spore producing microsporidia are ubiquitous, obligate, and intracellular fungus defined as emerging opportunistic pathogens of humans, livestock, companion animals, wild mammals, birds, and water worldwide. The occurrence of microsporidia in animals could be risky for human public health.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>33070189</pmid><doi>10.1093/mmy/myaa088</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-2072-8165</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1369-3786 |
| ispartof | Medical mycology (Oxford), 2020-10, Vol.59 (6), p.585-591 |
| issn | 1369-3786 1460-2709 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2452099777 |
| source | Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| title | Occurrence and molecular identification of zoonotic microsporidia in pet budgerigars (Melopsittacus undulatus) in Turkey |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T05%3A37%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Occurrence%20and%20molecular%20identification%20of%20zoonotic%20microsporidia%20in%20pet%20budgerigars%20(Melopsittacus%20undulatus)%20in%20Turkey&rft.jtitle=Medical%20mycology%20(Oxford)&rft.au=Pekmezci,%20Didem&rft.date=2020-10-17&rft.volume=59&rft.issue=6&rft.spage=585&rft.epage=591&rft.pages=585-591&rft.issn=1369-3786&rft.eissn=1460-2709&rft_id=info:doi/10.1093/mmy/myaa088&rft_dat=%3Cproquest_cross%3E2452099777%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2452099777&rft_id=info:pmid/33070189&rft_oup_id=10.1093/mmy/myaa088&rfr_iscdi=true |