Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats
Obesity is associated with altered glycine metabolism in humans. This study investigated the mechanisms regulating glycine metabolism in obese rats. Eight-week-old Zucker diabetic fatty rats (ZDF; a type-II diabetic animal model) received either 1% glycine or 1.19% l -alanine (isonitrogenous control...
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| Veröffentlicht in: | Amino acids 2020-10, Vol.52 (10), p.1413-1423 |
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| creator | Simmons, Rebecca M. McKnight, Sorin M. Edwards, Ashley K. Wu, Guoyao Satterfield, Michael C. |
| description | Obesity is associated with altered glycine metabolism in humans. This study investigated the mechanisms regulating glycine metabolism in obese rats. Eight-week-old Zucker diabetic fatty rats (ZDF; a type-II diabetic animal model) received either 1% glycine or 1.19%
l
-alanine (isonitrogenous control) in drinking water for 6 weeks. An additional group of lean Zucker rats also received 1.19%
l
-alanine as a lean control. Glycine concentrations in serum and liver were markedly lower in obese versus lean rats. Enteral glycine supplementation restored both serum and hepatic glycine levels, while reducing mesenteric and internal white fat mass compared with alanine-treated ZDF rats. Blood glucose and non-esterified fatty acid (NEFA) concentrations did not differ between the control and glycine-supplemented ZDF rats (
P
> 0.10). Both mRNA and protein expression of aminomethyltransferase (AMT) and glycine dehydrogenase, decarboxylating (GLDC) were increased in the livers of obese versus lean rats (
P
|
| doi_str_mv | 10.1007/s00726-020-02901-9 |
| format | Article |
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l
-alanine (isonitrogenous control) in drinking water for 6 weeks. An additional group of lean Zucker rats also received 1.19%
l
-alanine as a lean control. Glycine concentrations in serum and liver were markedly lower in obese versus lean rats. Enteral glycine supplementation restored both serum and hepatic glycine levels, while reducing mesenteric and internal white fat mass compared with alanine-treated ZDF rats. Blood glucose and non-esterified fatty acid (NEFA) concentrations did not differ between the control and glycine-supplemented ZDF rats (
P
> 0.10). Both mRNA and protein expression of aminomethyltransferase (AMT) and glycine dehydrogenase, decarboxylating (GLDC) were increased in the livers of obese versus lean rats (
P
< 0.05). In contrast, glycine cleavage system H (
GCSH
) hepatic mRNA expression was downregulated in obese versus lean rats, although there was no change in protein expression. These findings indicate that reduced quantities of glycine observed in obese subjects likely results from an upregulation of the hepatic glycine cleavage system and that dietary glycine supplementation potentially reduces obesity in ZDF rats.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-020-02901-9</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Adipose tissue ; Alanine ; Aminomethyltransferase ; Analytical Chemistry ; Animal models ; Biochemical Engineering ; Biochemistry ; Biomedical and Life Sciences ; Body fat ; Cleavage ; Dehydrogenases ; Diabetes ; Diabetes mellitus ; Dietary supplements ; Drinking water ; Esterification ; Fatty acids ; Gene expression ; Glycine ; Glycine dehydrogenase ; L-Alanine ; Life Sciences ; Liver ; Metabolism ; Neurobiology ; Obesity ; Original Article ; Protein expression ; Proteins ; Proteomics</subject><ispartof>Amino acids, 2020-10, Vol.52 (10), p.1413-1423</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-e217b3596b2600ab8b6a42336f50d01d5d4f41a58e7661a5b4e8a16153bfb0f43</citedby><cites>FETCH-LOGICAL-c352t-e217b3596b2600ab8b6a42336f50d01d5d4f41a58e7661a5b4e8a16153bfb0f43</cites><orcidid>0000-0002-9819-141X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00726-020-02901-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00726-020-02901-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Simmons, Rebecca M.</creatorcontrib><creatorcontrib>McKnight, Sorin M.</creatorcontrib><creatorcontrib>Edwards, Ashley K.</creatorcontrib><creatorcontrib>Wu, Guoyao</creatorcontrib><creatorcontrib>Satterfield, Michael C.</creatorcontrib><title>Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><description>Obesity is associated with altered glycine metabolism in humans. This study investigated the mechanisms regulating glycine metabolism in obese rats. Eight-week-old Zucker diabetic fatty rats (ZDF; a type-II diabetic animal model) received either 1% glycine or 1.19%
l
-alanine (isonitrogenous control) in drinking water for 6 weeks. An additional group of lean Zucker rats also received 1.19%
l
-alanine as a lean control. Glycine concentrations in serum and liver were markedly lower in obese versus lean rats. Enteral glycine supplementation restored both serum and hepatic glycine levels, while reducing mesenteric and internal white fat mass compared with alanine-treated ZDF rats. Blood glucose and non-esterified fatty acid (NEFA) concentrations did not differ between the control and glycine-supplemented ZDF rats (
P
> 0.10). Both mRNA and protein expression of aminomethyltransferase (AMT) and glycine dehydrogenase, decarboxylating (GLDC) were increased in the livers of obese versus lean rats (
P
< 0.05). In contrast, glycine cleavage system H (
GCSH
) hepatic mRNA expression was downregulated in obese versus lean rats, although there was no change in protein expression. These findings indicate that reduced quantities of glycine observed in obese subjects likely results from an upregulation of the hepatic glycine cleavage system and that dietary glycine supplementation potentially reduces obesity in ZDF rats.</description><subject>Adipose tissue</subject><subject>Alanine</subject><subject>Aminomethyltransferase</subject><subject>Analytical Chemistry</subject><subject>Animal models</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Body fat</subject><subject>Cleavage</subject><subject>Dehydrogenases</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Dietary supplements</subject><subject>Drinking water</subject><subject>Esterification</subject><subject>Fatty acids</subject><subject>Gene expression</subject><subject>Glycine</subject><subject>Glycine dehydrogenase</subject><subject>L-Alanine</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Metabolism</subject><subject>Neurobiology</subject><subject>Obesity</subject><subject>Original Article</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Proteomics</subject><issn>0939-4451</issn><issn>1438-2199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kb2O1TAQhS0EEpeFF6CKREMT8H-SEq1gQVppG2hoLDuZ3OslcYLHEeTFeD7mchFIFBT2FPPNmaM5jD0X_JXgvHmN9Elbc8npdVzU3QN2EFq1tRRd95AdeKe6WmsjHrMniPecC9kKe2A_7gJgLHsVU5_BI2B1gtWX2FfHae9jgmqA0z7k5QiJ2pVPQ-XnmJYZymmfSvYJR8jnFnxfMyDGJVXfTnGiyQjF5_2PEm7rOsEMqdACojIMW08biS6kPMR1IZkSETcgQ9Xnrf8CmWR8gLOj0Rdymn3Bp-zR6CeEZ7_rFfv07u3H6_f17d3Nh-s3t3WvjCw1SNEEZTobpOXchzZYr6VSdjR84GIwgx618KaFxlqqQUPrhRVGhTHwUasr9vKiu-bl6wZY3Byxh2nyCZYNnaSLtkYbZQh98Q96v2w5kTuiGmFM23BLlLxQfV4QM4xuzXGmIznB3TlKd4nSUZTuV5SuoyF1GUKC0xHyX-n_TP0EiE-m5Q</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Simmons, Rebecca M.</creator><creator>McKnight, Sorin M.</creator><creator>Edwards, Ashley K.</creator><creator>Wu, Guoyao</creator><creator>Satterfield, Michael C.</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9819-141X</orcidid></search><sort><creationdate>20201001</creationdate><title>Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats</title><author>Simmons, Rebecca M. ; McKnight, Sorin M. ; Edwards, Ashley K. ; Wu, Guoyao ; Satterfield, Michael C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-e217b3596b2600ab8b6a42336f50d01d5d4f41a58e7661a5b4e8a16153bfb0f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipose tissue</topic><topic>Alanine</topic><topic>Aminomethyltransferase</topic><topic>Analytical Chemistry</topic><topic>Animal models</topic><topic>Biochemical Engineering</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Body fat</topic><topic>Cleavage</topic><topic>Dehydrogenases</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Dietary supplements</topic><topic>Drinking water</topic><topic>Esterification</topic><topic>Fatty acids</topic><topic>Gene expression</topic><topic>Glycine</topic><topic>Glycine dehydrogenase</topic><topic>L-Alanine</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Metabolism</topic><topic>Neurobiology</topic><topic>Obesity</topic><topic>Original Article</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Proteomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simmons, Rebecca M.</creatorcontrib><creatorcontrib>McKnight, Sorin M.</creatorcontrib><creatorcontrib>Edwards, Ashley K.</creatorcontrib><creatorcontrib>Wu, Guoyao</creatorcontrib><creatorcontrib>Satterfield, Michael C.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Amino acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simmons, Rebecca M.</au><au>McKnight, Sorin M.</au><au>Edwards, Ashley K.</au><au>Wu, Guoyao</au><au>Satterfield, Michael C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats</atitle><jtitle>Amino acids</jtitle><stitle>Amino Acids</stitle><date>2020-10-01</date><risdate>2020</risdate><volume>52</volume><issue>10</issue><spage>1413</spage><epage>1423</epage><pages>1413-1423</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>Obesity is associated with altered glycine metabolism in humans. This study investigated the mechanisms regulating glycine metabolism in obese rats. Eight-week-old Zucker diabetic fatty rats (ZDF; a type-II diabetic animal model) received either 1% glycine or 1.19%
l
-alanine (isonitrogenous control) in drinking water for 6 weeks. An additional group of lean Zucker rats also received 1.19%
l
-alanine as a lean control. Glycine concentrations in serum and liver were markedly lower in obese versus lean rats. Enteral glycine supplementation restored both serum and hepatic glycine levels, while reducing mesenteric and internal white fat mass compared with alanine-treated ZDF rats. Blood glucose and non-esterified fatty acid (NEFA) concentrations did not differ between the control and glycine-supplemented ZDF rats (
P
> 0.10). Both mRNA and protein expression of aminomethyltransferase (AMT) and glycine dehydrogenase, decarboxylating (GLDC) were increased in the livers of obese versus lean rats (
P
< 0.05). In contrast, glycine cleavage system H (
GCSH
) hepatic mRNA expression was downregulated in obese versus lean rats, although there was no change in protein expression. These findings indicate that reduced quantities of glycine observed in obese subjects likely results from an upregulation of the hepatic glycine cleavage system and that dietary glycine supplementation potentially reduces obesity in ZDF rats.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00726-020-02901-9</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9819-141X</orcidid></addata></record> |
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| ispartof | Amino acids, 2020-10, Vol.52 (10), p.1413-1423 |
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| language | eng |
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| source | SpringerLink Journals |
| subjects | Adipose tissue Alanine Aminomethyltransferase Analytical Chemistry Animal models Biochemical Engineering Biochemistry Biomedical and Life Sciences Body fat Cleavage Dehydrogenases Diabetes Diabetes mellitus Dietary supplements Drinking water Esterification Fatty acids Gene expression Glycine Glycine dehydrogenase L-Alanine Life Sciences Liver Metabolism Neurobiology Obesity Original Article Protein expression Proteins Proteomics |
| title | Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats |
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