Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina
Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed t...
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| Veröffentlicht in: | Parasitology research (1987) 2020-12, Vol.119 (12), p.4049-4059 |
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| creator | Dorsch, Matias A. Regidor-Cerrillo, Javier Ortega-Mora, Luis M. Scioli, María V. Morrell, Eleonora L. Cantón, Germán J. Moore, Dadín P. Hecker, Yanina P. |
| description | Neospora caninum
is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of
N. caninum
in this region is limited. Therefore, this study aimed to genetically characterize
N. caninum
isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of
N. caninum
in the Humid Pampa. |
| doi_str_mv | 10.1007/s00436-020-06922-x |
| format | Article |
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is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of
N. caninum
in this region is limited. Therefore, this study aimed to genetically characterize
N. caninum
isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of
N. caninum
in the Humid Pampa.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-020-06922-x</identifier><identifier>PMID: 33057815</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Argentina ; Biomedical and Life Sciences ; Biomedicine ; Cattle ; Cattle Diseases - genetics ; Cattle Diseases - parasitology ; Coccidiosis - veterinary ; Evolution ; Female ; Fetus ; Fluorescent Antibody Technique, Indirect ; Genetic Variation - genetics ; Genetics ; Genotype ; Immunology ; Linkage Disequilibrium - genetics ; Medical Microbiology ; Microbiology ; Microsatellite Repeats - genetics ; Neospora - genetics ; Neospora - immunology ; Phylogeny - Original Paper ; Polymerase Chain Reaction ; Pregnancy</subject><ispartof>Parasitology research (1987), 2020-12, Vol.119 (12), p.4049-4059</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c262x-47365bca29a884bf8e77d7e6e239c644142d8eac35cd1fd30c199d76481f8a483</citedby><cites>FETCH-LOGICAL-c262x-47365bca29a884bf8e77d7e6e239c644142d8eac35cd1fd30c199d76481f8a483</cites><orcidid>0000-0001-6735-3810 ; 0000-0001-9316-0588 ; 0000-0002-7061-8629 ; 0000-0003-3494-8193</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-020-06922-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-020-06922-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33057815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dorsch, Matias A.</creatorcontrib><creatorcontrib>Regidor-Cerrillo, Javier</creatorcontrib><creatorcontrib>Ortega-Mora, Luis M.</creatorcontrib><creatorcontrib>Scioli, María V.</creatorcontrib><creatorcontrib>Morrell, Eleonora L.</creatorcontrib><creatorcontrib>Cantón, Germán J.</creatorcontrib><creatorcontrib>Moore, Dadín P.</creatorcontrib><creatorcontrib>Hecker, Yanina P.</creatorcontrib><title>Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Neospora caninum
is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of
N. caninum
in this region is limited. Therefore, this study aimed to genetically characterize
N. caninum
isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of
N. caninum
in the Humid Pampa.</description><subject>Animals</subject><subject>Argentina</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cattle</subject><subject>Cattle Diseases - genetics</subject><subject>Cattle Diseases - parasitology</subject><subject>Coccidiosis - veterinary</subject><subject>Evolution</subject><subject>Female</subject><subject>Fetus</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Genetic Variation - genetics</subject><subject>Genetics</subject><subject>Genotype</subject><subject>Immunology</subject><subject>Linkage Disequilibrium - genetics</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Microsatellite Repeats - genetics</subject><subject>Neospora - genetics</subject><subject>Neospora - immunology</subject><subject>Phylogeny - Original Paper</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAUhS0EgvL4AwzII0vAryTOiBAvidcAs-U6N8WosYPtVO3IP8elwMh0fXXPOdb5EDqm5IwSUp9HQgSvCsJIQaqGsWK5hSZUcFbQpiy30YQ0-U0o5XtoP8Z3QmhdCbGL9jgnZS1pOUGfD9YEH3WC-dwmwDNwPq0G62Y4wAL0PGJYJnDRLr6PkKzBbV5CtGmFrcNTv7AO8CP4OPigsdHOurHHXfA9Tm-A38betvhZ94POmTPr3dp2EXJask4fop0ufwNHP_MAvV5fvVzeFvdPN3eXF_eFYRVbFqLmVTk1mjVaSjHtJNR1W0MFjDcmt6KCtRK04aVpaddyYmjTtLmvpJ3UQvIDdLrJHYL_GCEm1dtocm3twI9RMVFSWbKqYlnKNtI1mhigU0OwvQ4rRYlao1cb9CqjV9_o1TKbTn7yx2kP7Z_ll3UW8I0g5pObQVDvfgwud_4v9gu8G5JW</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Dorsch, Matias A.</creator><creator>Regidor-Cerrillo, Javier</creator><creator>Ortega-Mora, Luis M.</creator><creator>Scioli, María V.</creator><creator>Morrell, Eleonora L.</creator><creator>Cantón, Germán J.</creator><creator>Moore, Dadín P.</creator><creator>Hecker, Yanina P.</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6735-3810</orcidid><orcidid>https://orcid.org/0000-0001-9316-0588</orcidid><orcidid>https://orcid.org/0000-0002-7061-8629</orcidid><orcidid>https://orcid.org/0000-0003-3494-8193</orcidid></search><sort><creationdate>20201201</creationdate><title>Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina</title><author>Dorsch, Matias A. ; Regidor-Cerrillo, Javier ; Ortega-Mora, Luis M. ; Scioli, María V. ; Morrell, Eleonora L. ; Cantón, Germán J. ; Moore, Dadín P. ; Hecker, Yanina P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c262x-47365bca29a884bf8e77d7e6e239c644142d8eac35cd1fd30c199d76481f8a483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Argentina</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cattle</topic><topic>Cattle Diseases - genetics</topic><topic>Cattle Diseases - parasitology</topic><topic>Coccidiosis - veterinary</topic><topic>Evolution</topic><topic>Female</topic><topic>Fetus</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Genetic Variation - genetics</topic><topic>Genetics</topic><topic>Genotype</topic><topic>Immunology</topic><topic>Linkage Disequilibrium - genetics</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Microsatellite Repeats - genetics</topic><topic>Neospora - genetics</topic><topic>Neospora - immunology</topic><topic>Phylogeny - Original Paper</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dorsch, Matias A.</creatorcontrib><creatorcontrib>Regidor-Cerrillo, Javier</creatorcontrib><creatorcontrib>Ortega-Mora, Luis M.</creatorcontrib><creatorcontrib>Scioli, María V.</creatorcontrib><creatorcontrib>Morrell, Eleonora L.</creatorcontrib><creatorcontrib>Cantón, Germán J.</creatorcontrib><creatorcontrib>Moore, Dadín P.</creatorcontrib><creatorcontrib>Hecker, Yanina P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dorsch, Matias A.</au><au>Regidor-Cerrillo, Javier</au><au>Ortega-Mora, Luis M.</au><au>Scioli, María V.</au><au>Morrell, Eleonora L.</au><au>Cantón, Germán J.</au><au>Moore, Dadín P.</au><au>Hecker, Yanina P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>119</volume><issue>12</issue><spage>4049</spage><epage>4059</epage><pages>4049-4059</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Neospora caninum
is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of
N. caninum
in this region is limited. Therefore, this study aimed to genetically characterize
N. caninum
isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of
N. caninum
in the Humid Pampa.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33057815</pmid><doi>10.1007/s00436-020-06922-x</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6735-3810</orcidid><orcidid>https://orcid.org/0000-0001-9316-0588</orcidid><orcidid>https://orcid.org/0000-0002-7061-8629</orcidid><orcidid>https://orcid.org/0000-0003-3494-8193</orcidid></addata></record> |
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| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2020-12, Vol.119 (12), p.4049-4059 |
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| subjects | Animals Argentina Biomedical and Life Sciences Biomedicine Cattle Cattle Diseases - genetics Cattle Diseases - parasitology Coccidiosis - veterinary Evolution Female Fetus Fluorescent Antibody Technique, Indirect Genetic Variation - genetics Genetics Genotype Immunology Linkage Disequilibrium - genetics Medical Microbiology Microbiology Microsatellite Repeats - genetics Neospora - genetics Neospora - immunology Phylogeny - Original Paper Polymerase Chain Reaction Pregnancy |
| title | Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
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