A fluorogenic probe using a catalytic reaction for the detection of trace intracellular zinc
Labile zinc plays various roles in cells at low concentrations which most fluorescent probes are not able to detect. Here we report a cephem-based probe which coordinates to zinc and zinc-bound water cleaves the scaffold and releases the fluorophore. In addition, the zinc is recycled and reacts with...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2020-11, Vol.56 (87), p.13327-1333 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Takashima, Ippei Inoue, Yohei Matsumoto, Nobuyuki Takagi, Akira Okuda, Kensuke |
| description | Labile zinc plays various roles in cells at low concentrations which most fluorescent probes are not able to detect. Here we report a cephem-based probe which coordinates to zinc and zinc-bound water cleaves the scaffold and releases the fluorophore. In addition, the zinc is recycled and reacts with multiple probes, amplifying the signal. This signal amplification system is useful for the detection of intracellular zinc at low concentrations and has potential for further development of probes with a similar molecular design.
A reaction-based fluorescent probe with cephem scaffold has been applied for signal amplification system to detect trace intracellular zinc. |
| doi_str_mv | 10.1039/d0cc05315e |
| format | Article |
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| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2020-11, Vol.56 (87), p.13327-1333 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2451850000 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Amplification beta-Lactams - chemistry Cell Line Fluorescent Dyes - chemistry Fluorescent indicators Intracellular Space - metabolism Limit of Detection Low concentrations Water - chemistry Zinc Zinc - chemistry Zinc - metabolism |
| title | A fluorogenic probe using a catalytic reaction for the detection of trace intracellular zinc |
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