Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres
Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized th...
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| Veröffentlicht in: | Acta biomaterialia 2020-12, Vol.118, p.141-152 |
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| creator | Gryadunova, Anna A. Koudan, Elizaveta V. Rodionov, Sergey A. Pereira, F.D.A.S. Meteleva, Nina Yu Kasyanov, Vladimir A. Parfenov, Vladislav A. Kovalev, Alexey V. Khesuani, Yusef D. Mironov, Vladimir A. Bulanova, Elena A. |
| description | Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.
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| doi_str_mv | 10.1016/j.actbio.2020.10.007 |
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[Display omitted]</description><subject>Actin</subject><subject>Actin Cytoskeleton</subject><subject>Biomechanical properties</subject><subject>Biomechanics</subject><subject>Chondrospheres</subject><subject>Cytochalasin D</subject><subject>Cytoskeleton</subject><subject>Filaments</subject><subject>Fusion</subject><subject>Intermediate Filaments</subject><subject>Mechanical properties</subject><subject>Microfilaments</subject><subject>Microtubules</subject><subject>Morphology</subject><subject>Nocodazole</subject><subject>Properties (attributes)</subject><subject>Spheroids</subject><subject>Spreading</subject><subject>Stiffness</subject><subject>Vimentin</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoO4uOvqPxAJeNlLz1Y-OslcBBlcFRa86Dl0JxUmY09nTNLC_HvTzOrBg3VJUTxvVapeQt4w2DBg6v6wGVwdY9pw4GtpA6CfkRtmtOl0r8zzlmvJOw2KXZOXpRwAhGHcvCDXQoDsJbAb4nfnmsoPnLCmmZZzqXgs1KW55jguFamPIWDGuU5nWhOte6RhmV2NaR4mGlduLtHRU04nzDVioSlQt0-zz6mc9k1bXpGrMEwFXz-9t-T7w8dvu8_d49dPX3YfHjsntlA777wMYmTSgXItgGvd46iC4aNgo-QAyJlmxsComOp58NIM_Rak0tIwI27J3aVv-8zPBUu1x1gcTtMwY1qK5bIHJY0RsqHv_kEPacltpZUyim3FVolGyQvl2i4lY7CnHI9DPlsGdnXBHuzFBbu6sFabC0329qn5Mh7R_xX9OXsD3l8AbNf4FTHb4iLODn3M6Kr1Kf5_wm8Exptb</recordid><startdate>202012</startdate><enddate>202012</enddate><creator>Gryadunova, Anna A.</creator><creator>Koudan, Elizaveta V.</creator><creator>Rodionov, Sergey A.</creator><creator>Pereira, F.D.A.S.</creator><creator>Meteleva, Nina Yu</creator><creator>Kasyanov, Vladimir A.</creator><creator>Parfenov, Vladislav A.</creator><creator>Kovalev, Alexey V.</creator><creator>Khesuani, Yusef D.</creator><creator>Mironov, Vladimir A.</creator><creator>Bulanova, Elena A.</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202012</creationdate><title>Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres</title><author>Gryadunova, Anna A. ; 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To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.
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| ispartof | Acta biomaterialia, 2020-12, Vol.118, p.141-152 |
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| subjects | Actin Actin Cytoskeleton Biomechanical properties Biomechanics Chondrospheres Cytochalasin D Cytoskeleton Filaments Fusion Intermediate Filaments Mechanical properties Microfilaments Microtubules Morphology Nocodazole Properties (attributes) Spheroids Spreading Stiffness Vimentin |
| title | Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres |
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