Structural characterization with light scattering: A tool for rationally designing protein formulations
Here we explore the possibility of using light scattering technologies as an analytical tool for understanding structural features of a protein that might be responsible for initiating aggregative interactions. Using widely independent complementary experimental and computational techniques, we foun...
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| Veröffentlicht in: | Analytical biochemistry 2020-11, Vol.609, p.113979-113979, Article 113979 |
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| container_end_page | 113979 |
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| container_title | Analytical biochemistry |
| container_volume | 609 |
| creator | Kumar, Atul Dubey, Richa Singhai, Sakshi Konar, Anita Dutt Basu, Anindya |
| description | Here we explore the possibility of using light scattering technologies as an analytical tool for understanding structural features of a protein that might be responsible for initiating aggregative interactions. Using widely independent complementary experimental and computational techniques, we found that interaction parameters like Km in particular possess good correlation with residue specific descriptors for the model protein Bovine Serum Albumin. Such information can help rationally design protein engineering and/or formulation strategies for prolonged shelf-life of such products.
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•Strategy to identify aggregation prone motifs of a protein.•Rational approach towards protein engineering & formulation design.•Correlate LS data with molecular simulations. |
| doi_str_mv | 10.1016/j.ab.2020.113979 |
| format | Article |
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•Strategy to identify aggregation prone motifs of a protein.•Rational approach towards protein engineering & formulation design.•Correlate LS data with molecular simulations.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2020.113979</identifier><identifier>PMID: 33035463</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Dynamic Light Scattering ; Hydrogen-Ion Concentration ; Kinetics ; Molecular dynamics ; Molecular Dynamics Simulation ; ProtDCal ; Protein Aggregates - physiology ; Protein aggregation ; Protein interaction parameters ; Protein Stability ; Serum Albumin, Bovine - chemistry ; Serum Albumin, Bovine - metabolism</subject><ispartof>Analytical biochemistry, 2020-11, Vol.609, p.113979-113979, Article 113979</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-a78c6a78868e8165dbcdfbda5ee4692efc56e637fac2797918b72e168b273dc43</citedby><cites>FETCH-LOGICAL-c350t-a78c6a78868e8165dbcdfbda5ee4692efc56e637fac2797918b72e168b273dc43</cites><orcidid>0000-0002-0166-7988 ; 0000-0001-7196-4614 ; 0000-0002-4512-0271</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2020.113979$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33035463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Atul</creatorcontrib><creatorcontrib>Dubey, Richa</creatorcontrib><creatorcontrib>Singhai, Sakshi</creatorcontrib><creatorcontrib>Konar, Anita Dutt</creatorcontrib><creatorcontrib>Basu, Anindya</creatorcontrib><title>Structural characterization with light scattering: A tool for rationally designing protein formulations</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Here we explore the possibility of using light scattering technologies as an analytical tool for understanding structural features of a protein that might be responsible for initiating aggregative interactions. Using widely independent complementary experimental and computational techniques, we found that interaction parameters like Km in particular possess good correlation with residue specific descriptors for the model protein Bovine Serum Albumin. Such information can help rationally design protein engineering and/or formulation strategies for prolonged shelf-life of such products.
[Display omitted]
•Strategy to identify aggregation prone motifs of a protein.•Rational approach towards protein engineering & formulation design.•Correlate LS data with molecular simulations.</description><subject>Animals</subject><subject>Cattle</subject><subject>Dynamic Light Scattering</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Molecular dynamics</subject><subject>Molecular Dynamics Simulation</subject><subject>ProtDCal</subject><subject>Protein Aggregates - physiology</subject><subject>Protein aggregation</subject><subject>Protein interaction parameters</subject><subject>Protein Stability</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Serum Albumin, Bovine - metabolism</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1PwzAMhiMEYmNw54Ry5NKRNG3a7jZNfEmTOADnKE3dLlPajiQFjV9P9gE3LrYsP35tvwhdUzKlhPK79VSW05jEoaSsyIoTNKak4BFhpDhFY0IIi2JeZCN04dyaEEqTlJ-jEWOEpQlnY9S8ejsoP1hpsFpJK5UHq7-l132Hv7RfYaOblcdOSb_rdM0Mz7Hve4Pr3mK7B6UxW1yB000XALyxvQfd7YB2MHvCXaKzWhoHV8c8Qe8P92-Lp2j58vi8mC8jxVLiI5nlioeQ8xxyytOqVFVdVjIFSHgRQ61SDpxltVRxFh6meZnFQHlexhmrVMIm6PagG474GMB50WqnwBjZQT84ESdJUaRhAQ0oOaDK9s5ZqMXG6lbaraBE7OwVayFLsbNXHOwNIzdH9aFsofob-PUzALMDAOHHTw1WOKWhU1BpC8qLqtf_q_8ARFGMRQ</recordid><startdate>20201115</startdate><enddate>20201115</enddate><creator>Kumar, Atul</creator><creator>Dubey, Richa</creator><creator>Singhai, Sakshi</creator><creator>Konar, Anita Dutt</creator><creator>Basu, Anindya</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0166-7988</orcidid><orcidid>https://orcid.org/0000-0001-7196-4614</orcidid><orcidid>https://orcid.org/0000-0002-4512-0271</orcidid></search><sort><creationdate>20201115</creationdate><title>Structural characterization with light scattering: A tool for rationally designing protein formulations</title><author>Kumar, Atul ; Dubey, Richa ; Singhai, Sakshi ; Konar, Anita Dutt ; Basu, Anindya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-a78c6a78868e8165dbcdfbda5ee4692efc56e637fac2797918b72e168b273dc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Dynamic Light Scattering</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Molecular dynamics</topic><topic>Molecular Dynamics Simulation</topic><topic>ProtDCal</topic><topic>Protein Aggregates - physiology</topic><topic>Protein aggregation</topic><topic>Protein interaction parameters</topic><topic>Protein Stability</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Serum Albumin, Bovine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Atul</creatorcontrib><creatorcontrib>Dubey, Richa</creatorcontrib><creatorcontrib>Singhai, Sakshi</creatorcontrib><creatorcontrib>Konar, Anita Dutt</creatorcontrib><creatorcontrib>Basu, Anindya</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Atul</au><au>Dubey, Richa</au><au>Singhai, Sakshi</au><au>Konar, Anita Dutt</au><au>Basu, Anindya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural characterization with light scattering: A tool for rationally designing protein formulations</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2020-11-15</date><risdate>2020</risdate><volume>609</volume><spage>113979</spage><epage>113979</epage><pages>113979-113979</pages><artnum>113979</artnum><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Here we explore the possibility of using light scattering technologies as an analytical tool for understanding structural features of a protein that might be responsible for initiating aggregative interactions. Using widely independent complementary experimental and computational techniques, we found that interaction parameters like Km in particular possess good correlation with residue specific descriptors for the model protein Bovine Serum Albumin. Such information can help rationally design protein engineering and/or formulation strategies for prolonged shelf-life of such products.
[Display omitted]
•Strategy to identify aggregation prone motifs of a protein.•Rational approach towards protein engineering & formulation design.•Correlate LS data with molecular simulations.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33035463</pmid><doi>10.1016/j.ab.2020.113979</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0166-7988</orcidid><orcidid>https://orcid.org/0000-0001-7196-4614</orcidid><orcidid>https://orcid.org/0000-0002-4512-0271</orcidid></addata></record> |
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| ispartof | Analytical biochemistry, 2020-11, Vol.609, p.113979-113979, Article 113979 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cattle Dynamic Light Scattering Hydrogen-Ion Concentration Kinetics Molecular dynamics Molecular Dynamics Simulation ProtDCal Protein Aggregates - physiology Protein aggregation Protein interaction parameters Protein Stability Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - metabolism |
| title | Structural characterization with light scattering: A tool for rationally designing protein formulations |
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