The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique
Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels h...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2021-07, Vol.109 (7), p.1209-1219 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Tian, Yinping Liu, Minyi Liu, Yaoyao Shi, Changzheng Wang, Yayu Liu, Tong Huang, Yi Zhong, Peihua Dai, Jian Liu, Xiangning |
| description | Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell‐loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano‐hydroxyapatite (na‐HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na‐HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na‐HA printing slurry to create a “bioink” to prepare SA/Gel/na‐HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na‐HA hydrogel exhibited shear‐thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na‐HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na‐HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction. |
| doi_str_mv | 10.1002/jbm.a.37114 |
| format | Article |
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Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell‐loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano‐hydroxyapatite (na‐HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na‐HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na‐HA printing slurry to create a “bioink” to prepare SA/Gel/na‐HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na‐HA hydrogel exhibited shear‐thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na‐HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na‐HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.37114</identifier><identifier>PMID: 33021062</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>3-D printers ; 3D bioprinting ; Alginates - chemistry ; Alginic acid ; Alkaline phosphatase ; Biocompatibility ; bioink ; Biomaterials ; Biomedical materials ; Bioprinting - methods ; Cell adhesion ; cell bioscaffolds ; Cell differentiation ; Cell proliferation ; Cell survival ; Cells, Cultured ; Compression ; Cytology ; Differentiation (biology) ; Durapatite - chemistry ; Gelatin ; Gelatin - chemistry ; human periodontal ligament stem cells ; Humans ; Hydrogels ; Hydrogels - chemistry ; Hydroxyapatite ; Ligaments ; Mechanical properties ; Modulus of elasticity ; Morphology ; Osteoblastogenesis ; Osteogenesis ; Periodontal ligament ; Periodontal Ligament - cytology ; Physical properties ; Printing ; Printing, Three-Dimensional ; Reconstruction ; Rheological properties ; Slurries ; Sodium alginate ; Stem cells ; Stem Cells - cytology ; Structure-function relationships ; Swelling ; Three dimensional printing ; Tissue Engineering ; Tissue Scaffolds - chemistry</subject><ispartof>Journal of biomedical materials research. Part A, 2021-07, Vol.109 (7), p.1209-1219</ispartof><rights>2020 Wiley Periodicals LLC</rights><rights>2020 Wiley Periodicals LLC.</rights><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4044-accfe1896d8434e19cc72b870ab964e628b3237333dd5e57542e5154a21702f3</citedby><cites>FETCH-LOGICAL-c4044-accfe1896d8434e19cc72b870ab964e628b3237333dd5e57542e5154a21702f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.37114$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.37114$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33021062$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Yinping</creatorcontrib><creatorcontrib>Liu, Minyi</creatorcontrib><creatorcontrib>Liu, Yaoyao</creatorcontrib><creatorcontrib>Shi, Changzheng</creatorcontrib><creatorcontrib>Wang, Yayu</creatorcontrib><creatorcontrib>Liu, Tong</creatorcontrib><creatorcontrib>Huang, Yi</creatorcontrib><creatorcontrib>Zhong, Peihua</creatorcontrib><creatorcontrib>Dai, Jian</creatorcontrib><creatorcontrib>Liu, Xiangning</creatorcontrib><title>The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique</title><title>Journal of biomedical materials research. Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell‐loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano‐hydroxyapatite (na‐HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na‐HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na‐HA printing slurry to create a “bioink” to prepare SA/Gel/na‐HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na‐HA hydrogel exhibited shear‐thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na‐HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na‐HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction.</description><subject>3-D printers</subject><subject>3D bioprinting</subject><subject>Alginates - chemistry</subject><subject>Alginic acid</subject><subject>Alkaline phosphatase</subject><subject>Biocompatibility</subject><subject>bioink</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Bioprinting - methods</subject><subject>Cell adhesion</subject><subject>cell bioscaffolds</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Cell survival</subject><subject>Cells, Cultured</subject><subject>Compression</subject><subject>Cytology</subject><subject>Differentiation (biology)</subject><subject>Durapatite - chemistry</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>human periodontal ligament stem cells</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Hydroxyapatite</subject><subject>Ligaments</subject><subject>Mechanical properties</subject><subject>Modulus of elasticity</subject><subject>Morphology</subject><subject>Osteoblastogenesis</subject><subject>Osteogenesis</subject><subject>Periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Physical properties</subject><subject>Printing</subject><subject>Printing, Three-Dimensional</subject><subject>Reconstruction</subject><subject>Rheological properties</subject><subject>Slurries</subject><subject>Sodium alginate</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Structure-function relationships</subject><subject>Swelling</subject><subject>Three dimensional printing</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90Dtv2zAUBWCiaFDn0al7QaBLgEAuefmQNCZu06RIkcU7QVFXtgyJdEQJQf59qNrNkKETOXz3kPcQ8oWzJWcMvu-qfmmXIudcfiCnXCnIZKnVx_kuy0xAqRfkLMZdwpop-EQWQjDgTMMp2ay3SPc4NGHorXdIQ0PFD1q1ITrbNKGrW7-hlY1Y0-CppdspuXmiDXXwo-1o125sj36kccSeOuw6uh9aP86DI7qtb58mvCAnje0ifj6e52R9-3O9usseHn_dr64fMieZlJl1rkFelLoupJDIS-dyqIqc2arUEjUUlQCRCyHqWqHKlQRUaU0LPGfQiHNyeYjdDyG9GkfTt3H-kvUYpmhAyqJQTBUs0W_v6C5Mg0-fM6AARClB66SuDsoNIcYBG5N26-3wYjgzc_0m1W-s-Vt_0l-PmVPVY_1m__WdABzAc9vhy_-yzO-bP9eH1Fd_Eo8a</recordid><startdate>202107</startdate><enddate>202107</enddate><creator>Tian, Yinping</creator><creator>Liu, Minyi</creator><creator>Liu, Yaoyao</creator><creator>Shi, Changzheng</creator><creator>Wang, Yayu</creator><creator>Liu, Tong</creator><creator>Huang, Yi</creator><creator>Zhong, Peihua</creator><creator>Dai, Jian</creator><creator>Liu, Xiangning</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202107</creationdate><title>The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique</title><author>Tian, Yinping ; 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Yinping</au><au>Liu, Minyi</au><au>Liu, Yaoyao</au><au>Shi, Changzheng</au><au>Wang, Yayu</au><au>Liu, Tong</au><au>Huang, Yi</au><au>Zhong, Peihua</au><au>Dai, Jian</au><au>Liu, Xiangning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J Biomed Mater Res A</addtitle><date>2021-07</date><risdate>2021</risdate><volume>109</volume><issue>7</issue><spage>1209</spage><epage>1219</epage><pages>1209-1219</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Bone tissue plays an important role in supporting and protecting the structure and function of the human body. Bone defects are a common source of injury and there are many reconstruction challenges in clinical practice. However, 3D bioprinting of scaffolds provides a promising solution. Hydrogels have emerged as biomaterials with good biocompatibility and are now widely used as cell‐loaded materials for bioprinting. This study involved three steps: First, sodium alginate (SA), gelatin (Gel), and nano‐hydroxyapatite (na‐HA) were mixed into a hydrogel and its rheological properties assessed to identify the optimum slurry for printing. Second, SA/Gel/na‐HA bioscaffolds were printed using 3D bioprinting technology and their physical properties characterized for surface morphology, swelling, and mechanical properties. Finally, human periodontal ligament stem cells (hPDLSCs) were mixed with SA/Gel/na‐HA printing slurry to create a “bioink” to prepare SA/Gel/na‐HA/ hPDLSCs cell bioscaffolds. These were tested for biocompatibility and osteogenic differentiation performance using live/dead cell staining, cell adhesion, cell proliferation, and alkaline phosphatase activity. The SA/Gel/na‐HA hydrogel exhibited shear‐thinning behavior. The equilibrium swelling of the bioscaffold was 125.9%, the compression stress was 0.671 MPa, and the compression elastic modulus was 8.27 MPa. The SA/Gel/na‐HA/hPDLSCs cell bioscaffolds caused effective stimulation of cell survival, proliferation, and osteoblast differentiation. Therefore, the SA/Gel/na‐HA/hPDLSCs cell bioscaffolds displayed potential as a material for bone defect reconstruction.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>33021062</pmid><doi>10.1002/jbm.a.37114</doi><tpages>11</tpages></addata></record> |
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| subjects | 3-D printers 3D bioprinting Alginates - chemistry Alginic acid Alkaline phosphatase Biocompatibility bioink Biomaterials Biomedical materials Bioprinting - methods Cell adhesion cell bioscaffolds Cell differentiation Cell proliferation Cell survival Cells, Cultured Compression Cytology Differentiation (biology) Durapatite - chemistry Gelatin Gelatin - chemistry human periodontal ligament stem cells Humans Hydrogels Hydrogels - chemistry Hydroxyapatite Ligaments Mechanical properties Modulus of elasticity Morphology Osteoblastogenesis Osteogenesis Periodontal ligament Periodontal Ligament - cytology Physical properties Printing Printing, Three-Dimensional Reconstruction Rheological properties Slurries Sodium alginate Stem cells Stem Cells - cytology Structure-function relationships Swelling Three dimensional printing Tissue Engineering Tissue Scaffolds - chemistry |
| title | The performance of 3D bioscaffolding based on a human periodontal ligament stem cell printing technique |
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