Functional and structural characterization of a novel GH3 β-glucosidase from the gut metagenome of the Brazilian Cerrado termite Syntermes wheeleri

In this study, a GH3 family β-glucosidase (Bgl7226) from metagenomic sequences of the Syntermes wheeleri gut, a Brazilian Cerrado termite, was expressed, purified and characterized. The enzyme showed two optimum pHs (pH 7 and pH 10), and a maximum optimum temperature of about 40 °C using 4-Nitrophen...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	International journal of biological macromolecules 2020-12, Vol.165 (Pt A), p.822-834
Hauptverfasser:	Lima, Raul Alcântara Teixeira, De Oliveira, Gideane, Souza, Amanda Araújo, Lopes, Fabyano Alvares Cardoso, Santana, Renata Henrique, Istvan, Paula, Quirino, Betania Ferraz, Barbosa, João, De Freitas, Sonia, Garay, Aisel Valle, Krüger, Ricardo Henrique
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals beta-Glucosidase - chemistry beta-Glucosidase - genetics Enzyme Stability Gastrointestinal Microbiome GH3-β-glucosidase Isoptera - microbiology Metagenome Substrate Specificity Syntermes wheeleri
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!