Wild boar as a potential reservoir of zoonotic tick-borne pathogens

The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic an...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Ticks and tick-borne diseases 2021-01, Vol.12 (1), p.101558-101558, Article 101558
Hauptverfasser: Hrazdilová, Kristýna, Lesiczka, Paulina Maria, Bardoň, Jan, Vyroubalová, Šárka, Šimek, Bronislav, Zurek, Ludek, Modrý, David
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 101558
container_issue 1
container_start_page 101558
container_title Ticks and tick-borne diseases
container_volume 12
creator Hrazdilová, Kristýna
Lesiczka, Paulina Maria
Bardoň, Jan
Vyroubalová, Šárka
Šimek, Bronislav
Zurek, Ludek
Modrý, David
description The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.
doi_str_mv 10.1016/j.ttbdis.2020.101558
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2448408097</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1877959X20304271</els_id><sourcerecordid>2448408097</sourcerecordid><originalsourceid>FETCH-LOGICAL-c428t-59cae21429a986718ed14fea88d86cb77c343d7351e8cfa67f58cb4c105bd6543</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMottT-A5EcvWxNNtlN9iJI8QsKXhS9hWwyq6nbTU3Sgv56t2716MAww_DOvMyD0CklM0poebGcpVRbF2c5yX9GRSEP0JhKIbKqJOzwty-qlxGaxrgkfTDKpciP0YgxQknJ6BjNn11rce11wDpijdc-QZecbnGACGHrXcC-wV_edz45g_t8z2ofOsBrnd78K3TxBB01uo0w3dcJerq5fpzfZYuH2_v51SIzPJcpKyqjIac8r3QlS0ElWMob0FJaWZpaCMM4s4IVFKRpdCmaQpqaG0qK2pYFZxN0PtxdB_-xgZjUykUDbas78Juocs4lJ5JUopfyQWqCjzFAo9bBrXT4VJSoHUG1VANBtSOoBoL92tneYVOvwP4t_fLqBZeDAPo_tw6CisZBZ8C6ACYp693_Dt_VZoMQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2448408097</pqid></control><display><type>article</type><title>Wild boar as a potential reservoir of zoonotic tick-borne pathogens</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Hrazdilová, Kristýna ; Lesiczka, Paulina Maria ; Bardoň, Jan ; Vyroubalová, Šárka ; Šimek, Bronislav ; Zurek, Ludek ; Modrý, David</creator><creatorcontrib>Hrazdilová, Kristýna ; Lesiczka, Paulina Maria ; Bardoň, Jan ; Vyroubalová, Šárka ; Šimek, Bronislav ; Zurek, Ludek ; Modrý, David</creatorcontrib><description>The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.</description><identifier>ISSN: 1877-959X</identifier><identifier>EISSN: 1877-9603</identifier><identifier>DOI: 10.1016/j.ttbdis.2020.101558</identifier><identifier>PMID: 33010631</identifier><language>eng</language><publisher>Netherlands: Elsevier GmbH</publisher><subject>Anaplasma phagocytophilum ; Ixodesricinus ; Piroplasmids ; Wild boar ; Zoonosis</subject><ispartof>Ticks and tick-borne diseases, 2021-01, Vol.12 (1), p.101558-101558, Article 101558</ispartof><rights>2020 Elsevier GmbH</rights><rights>Copyright © 2020 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-59cae21429a986718ed14fea88d86cb77c343d7351e8cfa67f58cb4c105bd6543</citedby><cites>FETCH-LOGICAL-c428t-59cae21429a986718ed14fea88d86cb77c343d7351e8cfa67f58cb4c105bd6543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ttbdis.2020.101558$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33010631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hrazdilová, Kristýna</creatorcontrib><creatorcontrib>Lesiczka, Paulina Maria</creatorcontrib><creatorcontrib>Bardoň, Jan</creatorcontrib><creatorcontrib>Vyroubalová, Šárka</creatorcontrib><creatorcontrib>Šimek, Bronislav</creatorcontrib><creatorcontrib>Zurek, Ludek</creatorcontrib><creatorcontrib>Modrý, David</creatorcontrib><title>Wild boar as a potential reservoir of zoonotic tick-borne pathogens</title><title>Ticks and tick-borne diseases</title><addtitle>Ticks Tick Borne Dis</addtitle><description>The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.</description><subject>Anaplasma phagocytophilum</subject><subject>Ixodesricinus</subject><subject>Piroplasmids</subject><subject>Wild boar</subject><subject>Zoonosis</subject><issn>1877-959X</issn><issn>1877-9603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMottT-A5EcvWxNNtlN9iJI8QsKXhS9hWwyq6nbTU3Sgv56t2716MAww_DOvMyD0CklM0poebGcpVRbF2c5yX9GRSEP0JhKIbKqJOzwty-qlxGaxrgkfTDKpciP0YgxQknJ6BjNn11rce11wDpijdc-QZecbnGACGHrXcC-wV_edz45g_t8z2ofOsBrnd78K3TxBB01uo0w3dcJerq5fpzfZYuH2_v51SIzPJcpKyqjIac8r3QlS0ElWMob0FJaWZpaCMM4s4IVFKRpdCmaQpqaG0qK2pYFZxN0PtxdB_-xgZjUykUDbas78Juocs4lJ5JUopfyQWqCjzFAo9bBrXT4VJSoHUG1VANBtSOoBoL92tneYVOvwP4t_fLqBZeDAPo_tw6CisZBZ8C6ACYp693_Dt_VZoMQ</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Hrazdilová, Kristýna</creator><creator>Lesiczka, Paulina Maria</creator><creator>Bardoň, Jan</creator><creator>Vyroubalová, Šárka</creator><creator>Šimek, Bronislav</creator><creator>Zurek, Ludek</creator><creator>Modrý, David</creator><general>Elsevier GmbH</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202101</creationdate><title>Wild boar as a potential reservoir of zoonotic tick-borne pathogens</title><author>Hrazdilová, Kristýna ; Lesiczka, Paulina Maria ; Bardoň, Jan ; Vyroubalová, Šárka ; Šimek, Bronislav ; Zurek, Ludek ; Modrý, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-59cae21429a986718ed14fea88d86cb77c343d7351e8cfa67f58cb4c105bd6543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anaplasma phagocytophilum</topic><topic>Ixodesricinus</topic><topic>Piroplasmids</topic><topic>Wild boar</topic><topic>Zoonosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hrazdilová, Kristýna</creatorcontrib><creatorcontrib>Lesiczka, Paulina Maria</creatorcontrib><creatorcontrib>Bardoň, Jan</creatorcontrib><creatorcontrib>Vyroubalová, Šárka</creatorcontrib><creatorcontrib>Šimek, Bronislav</creatorcontrib><creatorcontrib>Zurek, Ludek</creatorcontrib><creatorcontrib>Modrý, David</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ticks and tick-borne diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hrazdilová, Kristýna</au><au>Lesiczka, Paulina Maria</au><au>Bardoň, Jan</au><au>Vyroubalová, Šárka</au><au>Šimek, Bronislav</au><au>Zurek, Ludek</au><au>Modrý, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wild boar as a potential reservoir of zoonotic tick-borne pathogens</atitle><jtitle>Ticks and tick-borne diseases</jtitle><addtitle>Ticks Tick Borne Dis</addtitle><date>2021-01</date><risdate>2021</risdate><volume>12</volume><issue>1</issue><spage>101558</spage><epage>101558</epage><pages>101558-101558</pages><artnum>101558</artnum><issn>1877-959X</issn><eissn>1877-9603</eissn><abstract>The wild boar (Sus scrofa) population has increased dramatically over the last decades throughout Europe and it has become a serious pest. In addition, the common habitat of wild boar and of the tick, Ixodes ricinus, indicates the potential of wild boar to play a role in epidemiology of epizootic and zoonotic tick-borne pathogens, including Anaplasma phagocytophilum. In Europe, epidemiological cycles and reservoirs of A. phagocytophilum, including its zoonotic haplotypes, are poorly understood. In this study, we focused on detection and further genetic characterization of A. phagocytophilum and piroplasmids in 550 wild boars from eleven districts of Moravia and Silesia in the Czech Republic. Using highly sensitive nested PCR targeting the groEL gene, the DNA of A. phagocytophilum was detected in 28 wild boars (5.1 %) representing six unique haplotypes. The dominant haplotype was found in 21 samples from 7 different districts. All detected haplotypes clustered in the largest clade representing the European ecotype I and the dominant haplotype fell to the subclade with the European human cases and strains from dogs and horses. Nested PCR targeting the variable region of the 18S rRNA gene of piroplasmids resulted in one positive sample with 99.8 % sequence identity to Babesia divergens. The presence of these two pathogens that are primarily circulated by I. ricinus confirms the local participation of wild boar in the host spectrum of this tick and warrants experimental studies to address wild boar as a reservoir of zoonotic haplotypes of A. phagocytophilum.</abstract><cop>Netherlands</cop><pub>Elsevier GmbH</pub><pmid>33010631</pmid><doi>10.1016/j.ttbdis.2020.101558</doi><tpages>1</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1877-959X
ispartof Ticks and tick-borne diseases, 2021-01, Vol.12 (1), p.101558-101558, Article 101558
issn 1877-959X
1877-9603
language eng
recordid cdi_proquest_miscellaneous_2448408097
source Elsevier ScienceDirect Journals Complete
subjects Anaplasma phagocytophilum
Ixodesricinus
Piroplasmids
Wild boar
Zoonosis
title Wild boar as a potential reservoir of zoonotic tick-borne pathogens
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T05%3A12%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Wild%20boar%20as%20a%20potential%20reservoir%20of%20zoonotic%20tick-borne%20pathogens&rft.jtitle=Ticks%20and%20tick-borne%20diseases&rft.au=Hrazdilov%C3%A1,%20Krist%C3%BDna&rft.date=2021-01&rft.volume=12&rft.issue=1&rft.spage=101558&rft.epage=101558&rft.pages=101558-101558&rft.artnum=101558&rft.issn=1877-959X&rft.eissn=1877-9603&rft_id=info:doi/10.1016/j.ttbdis.2020.101558&rft_dat=%3Cproquest_cross%3E2448408097%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2448408097&rft_id=info:pmid/33010631&rft_els_id=S1877959X20304271&rfr_iscdi=true