Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity
•Two rescued viruses rGX15MM6−2and rGX14FF03 and a recombinant virus rALV-B-Jenv were generated and tested utilizing both in vitro and in vivo experiments.•The env gene affects virus replication in vitro, and affects viremia, tumor type in vivo.•Recombinant subgroup B avian leukosis virus combined w...
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| Veröffentlicht in: | Veterinary microbiology 2020-11, Vol.250, p.108862-108862, Article 108862 |
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| container_title | Veterinary microbiology |
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| creator | Li, Qiuhong Wang, Peikun Li, Min Lin, Lulu Shi, Mengya Li, Haijuan Deng, Qiaomu Teng, Huang Mo, Meilan Wei, Tianchao Wei, Ping |
| description | •Two rescued viruses rGX15MM6−2and rGX14FF03 and a recombinant virus rALV-B-Jenv were generated and tested utilizing both in vitro and in vivo experiments.•The env gene affects virus replication in vitro, and affects viremia, tumor type in vivo.•Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity.
The differences among different sub-groups of the avian leukosis virus (ALV) genome are mainly concentrated in the env gene, which binds to cell-specific receptors and determines the characteristics of viral tropism and pathogenicity. In this study, two rescued viruses rGX15MM6−2 (ALV of subgroup J, ALV-J) and rGX14FF03 (ALV of subgroup B, ALV-B) and a recombinant virus rALV-B-Jenv (ALV-B’s backbone with ALV-J’s env) were generated and tested utilizing both in vitro and in vivo experiments. The results showed that the replication ability of the viruses released in DF-1 cell cultures was listed in order as rGX15MM6−2 >rALV-B-Jenv >rGX14FF03. rGX15MM6−2 caused the most serious suppression of body weight gain, exhibited a significant negative effect on the development of immune organs (P < 0.05) and lower antibody responses to vaccinations with the commercial oil-emulsion vaccines (OEVs) (P |
| doi_str_mv | 10.1016/j.vetmic.2020.108862 |
| format | Article |
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The differences among different sub-groups of the avian leukosis virus (ALV) genome are mainly concentrated in the env gene, which binds to cell-specific receptors and determines the characteristics of viral tropism and pathogenicity. In this study, two rescued viruses rGX15MM6−2 (ALV of subgroup J, ALV-J) and rGX14FF03 (ALV of subgroup B, ALV-B) and a recombinant virus rALV-B-Jenv (ALV-B’s backbone with ALV-J’s env) were generated and tested utilizing both in vitro and in vivo experiments. The results showed that the replication ability of the viruses released in DF-1 cell cultures was listed in order as rGX15MM6−2 >rALV-B-Jenv >rGX14FF03. rGX15MM6−2 caused the most serious suppression of body weight gain, exhibited a significant negative effect on the development of immune organs (P < 0.05) and lower antibody responses to vaccinations with the commercial oil-emulsion vaccines (OEVs) (P<0.05) in the challenged chickens. The viral detection showed that the positive rate in blood from the birds infected with rALV-B-Jenv were respectively higher than those from the birds infected with rGX14FF03 (P < 0.05). At 25 wpi, similar tumors were found in the abdominal cavity of the birds in rGX15MM6−2 and rALV-B-Jenv groups. The results demonstrated that the ALV-J env gene significantly increases the pathogenicity of the recombinant ALV-B. With the increasing incidence of co-infections of different subgroups of ALV in the field, the possibility of viral recombination is increasing and demands further study.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2020.108862</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Avian leukosis ; Avian leukosis virus of subgroup B ; Avian leukosis virus of subgroup J ; Body weight gain ; Env ; Env gene ; Genomes ; Leukosis ; Pathogenesis ; Pathogenicity ; Recombinant virus ; Recombination ; Tropism ; Tumors ; Viruses</subject><ispartof>Veterinary microbiology, 2020-11, Vol.250, p.108862-108862, Article 108862</ispartof><rights>2020 Elsevier B.V.</rights><rights>Copyright Elsevier BV Nov 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-9ac09b4ad6d3b9ba5605fe3901aa47a5f50932847def90619b9163045de387653</citedby><cites>FETCH-LOGICAL-c367t-9ac09b4ad6d3b9ba5605fe3901aa47a5f50932847def90619b9163045de387653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2020.108862$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Li, Qiuhong</creatorcontrib><creatorcontrib>Wang, Peikun</creatorcontrib><creatorcontrib>Li, Min</creatorcontrib><creatorcontrib>Lin, Lulu</creatorcontrib><creatorcontrib>Shi, Mengya</creatorcontrib><creatorcontrib>Li, Haijuan</creatorcontrib><creatorcontrib>Deng, Qiaomu</creatorcontrib><creatorcontrib>Teng, Huang</creatorcontrib><creatorcontrib>Mo, Meilan</creatorcontrib><creatorcontrib>Wei, Tianchao</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><title>Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity</title><title>Veterinary microbiology</title><description>•Two rescued viruses rGX15MM6−2and rGX14FF03 and a recombinant virus rALV-B-Jenv were generated and tested utilizing both in vitro and in vivo experiments.•The env gene affects virus replication in vitro, and affects viremia, tumor type in vivo.•Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity.
The differences among different sub-groups of the avian leukosis virus (ALV) genome are mainly concentrated in the env gene, which binds to cell-specific receptors and determines the characteristics of viral tropism and pathogenicity. In this study, two rescued viruses rGX15MM6−2 (ALV of subgroup J, ALV-J) and rGX14FF03 (ALV of subgroup B, ALV-B) and a recombinant virus rALV-B-Jenv (ALV-B’s backbone with ALV-J’s env) were generated and tested utilizing both in vitro and in vivo experiments. The results showed that the replication ability of the viruses released in DF-1 cell cultures was listed in order as rGX15MM6−2 >rALV-B-Jenv >rGX14FF03. rGX15MM6−2 caused the most serious suppression of body weight gain, exhibited a significant negative effect on the development of immune organs (P < 0.05) and lower antibody responses to vaccinations with the commercial oil-emulsion vaccines (OEVs) (P<0.05) in the challenged chickens. The viral detection showed that the positive rate in blood from the birds infected with rALV-B-Jenv were respectively higher than those from the birds infected with rGX14FF03 (P < 0.05). At 25 wpi, similar tumors were found in the abdominal cavity of the birds in rGX15MM6−2 and rALV-B-Jenv groups. The results demonstrated that the ALV-J env gene significantly increases the pathogenicity of the recombinant ALV-B. With the increasing incidence of co-infections of different subgroups of ALV in the field, the possibility of viral recombination is increasing and demands further study.</description><subject>Avian leukosis</subject><subject>Avian leukosis virus of subgroup B</subject><subject>Avian leukosis virus of subgroup J</subject><subject>Body weight gain</subject><subject>Env</subject><subject>Env gene</subject><subject>Genomes</subject><subject>Leukosis</subject><subject>Pathogenesis</subject><subject>Pathogenicity</subject><subject>Recombinant virus</subject><subject>Recombination</subject><subject>Tropism</subject><subject>Tumors</subject><subject>Viruses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kU2L1EAQhoMoOK7-Aw8NXrxk7O-kL4Iu6xcLC8t6bjqdykyNmc7Y3YkM_nl7iCB42FNB8bwvVTxV9ZrRLaNMvztsF8hH9FtO-WXVtpo_qTasbUTNleRPqw0VTVszJtTz6kVKB0qpNJpuqt_34Kdjh8GFTNLc7eI0n8hH4hZ0gYww_5gSJrJgnBNZSejJL8x7kvfwL_GNQFjIDkLZ4S7ggL40jmeCwUdwCRLBnMjJ5f1UKPSYzy-rZ4MbE7z6O6-q759uHq6_1Ld3n79ef7itvdBNro3z1HTS9boXnemc0lQNIAxlzsnGqUFRI3grmx4GQzUznWFaUKl6EG2jlbiq3q69pzj9nCFle8TkYRxdgGlOlkvZSqq1ogV98x96mOYYynWF0oYZyrkslFwpH6eUIgz2FPHo4tkyai9G7MGuRuzFiF2NlNj7NQbl2QUh2uQRgoceI_hs-wkfL_gDmNCXog</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Li, Qiuhong</creator><creator>Wang, Peikun</creator><creator>Li, Min</creator><creator>Lin, Lulu</creator><creator>Shi, Mengya</creator><creator>Li, Haijuan</creator><creator>Deng, Qiaomu</creator><creator>Teng, Huang</creator><creator>Mo, Meilan</creator><creator>Wei, Tianchao</creator><creator>Wei, Ping</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>202011</creationdate><title>Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity</title><author>Li, Qiuhong ; Wang, Peikun ; Li, Min ; Lin, Lulu ; Shi, Mengya ; Li, Haijuan ; Deng, Qiaomu ; Teng, Huang ; Mo, Meilan ; Wei, Tianchao ; Wei, Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-9ac09b4ad6d3b9ba5605fe3901aa47a5f50932847def90619b9163045de387653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Avian leukosis</topic><topic>Avian leukosis virus of subgroup B</topic><topic>Avian leukosis virus of subgroup J</topic><topic>Body weight gain</topic><topic>Env</topic><topic>Env gene</topic><topic>Genomes</topic><topic>Leukosis</topic><topic>Pathogenesis</topic><topic>Pathogenicity</topic><topic>Recombinant virus</topic><topic>Recombination</topic><topic>Tropism</topic><topic>Tumors</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Qiuhong</creatorcontrib><creatorcontrib>Wang, Peikun</creatorcontrib><creatorcontrib>Li, Min</creatorcontrib><creatorcontrib>Lin, Lulu</creatorcontrib><creatorcontrib>Shi, Mengya</creatorcontrib><creatorcontrib>Li, Haijuan</creatorcontrib><creatorcontrib>Deng, Qiaomu</creatorcontrib><creatorcontrib>Teng, Huang</creatorcontrib><creatorcontrib>Mo, Meilan</creatorcontrib><creatorcontrib>Wei, Tianchao</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Qiuhong</au><au>Wang, Peikun</au><au>Li, Min</au><au>Lin, Lulu</au><au>Shi, Mengya</au><au>Li, Haijuan</au><au>Deng, Qiaomu</au><au>Teng, Huang</au><au>Mo, Meilan</au><au>Wei, Tianchao</au><au>Wei, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity</atitle><jtitle>Veterinary microbiology</jtitle><date>2020-11</date><risdate>2020</risdate><volume>250</volume><spage>108862</spage><epage>108862</epage><pages>108862-108862</pages><artnum>108862</artnum><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>•Two rescued viruses rGX15MM6−2and rGX14FF03 and a recombinant virus rALV-B-Jenv were generated and tested utilizing both in vitro and in vivo experiments.•The env gene affects virus replication in vitro, and affects viremia, tumor type in vivo.•Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity.
The differences among different sub-groups of the avian leukosis virus (ALV) genome are mainly concentrated in the env gene, which binds to cell-specific receptors and determines the characteristics of viral tropism and pathogenicity. In this study, two rescued viruses rGX15MM6−2 (ALV of subgroup J, ALV-J) and rGX14FF03 (ALV of subgroup B, ALV-B) and a recombinant virus rALV-B-Jenv (ALV-B’s backbone with ALV-J’s env) were generated and tested utilizing both in vitro and in vivo experiments. The results showed that the replication ability of the viruses released in DF-1 cell cultures was listed in order as rGX15MM6−2 >rALV-B-Jenv >rGX14FF03. rGX15MM6−2 caused the most serious suppression of body weight gain, exhibited a significant negative effect on the development of immune organs (P < 0.05) and lower antibody responses to vaccinations with the commercial oil-emulsion vaccines (OEVs) (P<0.05) in the challenged chickens. The viral detection showed that the positive rate in blood from the birds infected with rALV-B-Jenv were respectively higher than those from the birds infected with rGX14FF03 (P < 0.05). At 25 wpi, similar tumors were found in the abdominal cavity of the birds in rGX15MM6−2 and rALV-B-Jenv groups. The results demonstrated that the ALV-J env gene significantly increases the pathogenicity of the recombinant ALV-B. With the increasing incidence of co-infections of different subgroups of ALV in the field, the possibility of viral recombination is increasing and demands further study.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.vetmic.2020.108862</doi><tpages>1</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 2020-11, Vol.250, p.108862-108862, Article 108862 |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Avian leukosis Avian leukosis virus of subgroup B Avian leukosis virus of subgroup J Body weight gain Env Env gene Genomes Leukosis Pathogenesis Pathogenicity Recombinant virus Recombination Tropism Tumors Viruses |
| title | Recombinant subgroup B avian leukosis virus combined with the subgroup J env gene significantly increases its pathogenicity |
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