A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding
Schistosomiasis is a neglected tropical disease that infects 240 million people. With no vaccines and only one drug available, new therapeutic targets are needed. The causative agents, schistosomes, are intravascular flatworm parasites that feed on blood and lay eggs, resulting in pathology. The fun...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2020-09, Vol.369 (6511), p.1644-1649 |
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| creator | Wendt, George Zhao, Lu Chen, Rui Liu, Chenxi O'Donoghue, Anthony J Caffrey, Conor R Reese, Michael L Collins, 3rd, James J |
| description | Schistosomiasis is a neglected tropical disease that infects 240 million people. With no vaccines and only one drug available, new therapeutic targets are needed. The causative agents, schistosomes, are intravascular flatworm parasites that feed on blood and lay eggs, resulting in pathology. The function of the parasite's various tissues in successful parasitism are poorly understood, hindering identification of therapeutic targets. Using single-cell RNA sequencing (RNA-seq), we characterize 43,642 cells from the adult schistosome and identify 68 distinct cell populations, including specialized stem cells that maintain the parasite's blood-digesting gut. These stem cells express the gene
, which is required for gut maintenance, blood feeding, and pathology in vivo. Together, these data provide molecular insights into the organ systems of this important pathogen and identify potential therapeutic targets. |
| doi_str_mv | 10.1126/science.abb7709 |
| format | Article |
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, which is required for gut maintenance, blood feeding, and pathology in vivo. Together, these data provide molecular insights into the organ systems of this important pathogen and identify potential therapeutic targets.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.abb7709</identifier><identifier>PMID: 32973030</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>Animals ; Atlases as Topic ; Blood ; Blood - parasitology ; Eggs ; Female ; Gene Expression ; Gene sequencing ; Helminth Proteins - genetics ; Helminth Proteins - physiology ; Hepatocyte Nuclear Factor 4 - genetics ; Hepatocyte Nuclear Factor 4 - physiology ; Interference ; Kinases ; Male ; Parasites ; Parasitism ; Pathology ; Protein kinase ; Ribonucleic acid ; RNA ; RNA Interference ; RNA-mediated interference ; RNA-Seq ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Schistosoma mansoni - physiology ; Schistosomiasis ; Schistosomiasis mansoni - blood ; Schistosomiasis mansoni - parasitology ; Single-Cell Analysis ; Stem cell transplantation ; Stem cells ; Stem Cells - metabolism ; Target recognition ; Therapeutic applications ; Tropical diseases ; Vaccines</subject><ispartof>Science (American Association for the Advancement of Science), 2020-09, Vol.369 (6511), p.1644-1649</ispartof><rights>Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.</rights><rights>Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-d1c8b3df02ad44b1e0ea7a3f8d178c1a619804e2b174c03f31d7a759d41c93003</citedby><cites>FETCH-LOGICAL-c366t-d1c8b3df02ad44b1e0ea7a3f8d178c1a619804e2b174c03f31d7a759d41c93003</cites><orcidid>0000-0003-4996-660X ; 0000-0002-8302-079X ; 0000-0003-0142-553X ; 0000-0001-9401-9594 ; 0000-0002-8803-2095 ; 0000-0001-5237-1004 ; 0000-0001-5695-0409</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2884,2885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32973030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wendt, George</creatorcontrib><creatorcontrib>Zhao, Lu</creatorcontrib><creatorcontrib>Chen, Rui</creatorcontrib><creatorcontrib>Liu, Chenxi</creatorcontrib><creatorcontrib>O'Donoghue, Anthony J</creatorcontrib><creatorcontrib>Caffrey, Conor R</creatorcontrib><creatorcontrib>Reese, Michael L</creatorcontrib><creatorcontrib>Collins, 3rd, James J</creatorcontrib><title>A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Schistosomiasis is a neglected tropical disease that infects 240 million people. With no vaccines and only one drug available, new therapeutic targets are needed. The causative agents, schistosomes, are intravascular flatworm parasites that feed on blood and lay eggs, resulting in pathology. The function of the parasite's various tissues in successful parasitism are poorly understood, hindering identification of therapeutic targets. Using single-cell RNA sequencing (RNA-seq), we characterize 43,642 cells from the adult schistosome and identify 68 distinct cell populations, including specialized stem cells that maintain the parasite's blood-digesting gut. These stem cells express the gene
, which is required for gut maintenance, blood feeding, and pathology in vivo. Together, these data provide molecular insights into the organ systems of this important pathogen and identify potential therapeutic targets.</description><subject>Animals</subject><subject>Atlases as Topic</subject><subject>Blood</subject><subject>Blood - parasitology</subject><subject>Eggs</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene sequencing</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - physiology</subject><subject>Hepatocyte Nuclear Factor 4 - genetics</subject><subject>Hepatocyte Nuclear Factor 4 - physiology</subject><subject>Interference</subject><subject>Kinases</subject><subject>Male</subject><subject>Parasites</subject><subject>Parasitism</subject><subject>Pathology</subject><subject>Protein kinase</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA-mediated interference</subject><subject>RNA-Seq</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - 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Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wendt, George</au><au>Zhao, Lu</au><au>Chen, Rui</au><au>Liu, Chenxi</au><au>O'Donoghue, Anthony J</au><au>Caffrey, Conor R</au><au>Reese, Michael L</au><au>Collins, 3rd, James J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>2020-09-25</date><risdate>2020</risdate><volume>369</volume><issue>6511</issue><spage>1644</spage><epage>1649</epage><pages>1644-1649</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Schistosomiasis is a neglected tropical disease that infects 240 million people. With no vaccines and only one drug available, new therapeutic targets are needed. The causative agents, schistosomes, are intravascular flatworm parasites that feed on blood and lay eggs, resulting in pathology. The function of the parasite's various tissues in successful parasitism are poorly understood, hindering identification of therapeutic targets. Using single-cell RNA sequencing (RNA-seq), we characterize 43,642 cells from the adult schistosome and identify 68 distinct cell populations, including specialized stem cells that maintain the parasite's blood-digesting gut. These stem cells express the gene
, which is required for gut maintenance, blood feeding, and pathology in vivo. Together, these data provide molecular insights into the organ systems of this important pathogen and identify potential therapeutic targets.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>32973030</pmid><doi>10.1126/science.abb7709</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-4996-660X</orcidid><orcidid>https://orcid.org/0000-0002-8302-079X</orcidid><orcidid>https://orcid.org/0000-0003-0142-553X</orcidid><orcidid>https://orcid.org/0000-0001-9401-9594</orcidid><orcidid>https://orcid.org/0000-0002-8803-2095</orcidid><orcidid>https://orcid.org/0000-0001-5237-1004</orcidid><orcidid>https://orcid.org/0000-0001-5695-0409</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Atlases as Topic Blood Blood - parasitology Eggs Female Gene Expression Gene sequencing Helminth Proteins - genetics Helminth Proteins - physiology Hepatocyte Nuclear Factor 4 - genetics Hepatocyte Nuclear Factor 4 - physiology Interference Kinases Male Parasites Parasitism Pathology Protein kinase Ribonucleic acid RNA RNA Interference RNA-mediated interference RNA-Seq Schistosoma mansoni Schistosoma mansoni - genetics Schistosoma mansoni - physiology Schistosomiasis Schistosomiasis mansoni - blood Schistosomiasis mansoni - parasitology Single-Cell Analysis Stem cell transplantation Stem cells Stem Cells - metabolism Target recognition Therapeutic applications Tropical diseases Vaccines |
| title | A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding |
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