Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves

Background The present study reports the development of a sensitive dot blot protocol for determining the level of preformed antibodies against porcine heart valve tissue derived from wild‐type (WT) and α‐Gal‐KO (GGTA1‐KO) pigs in human sera. Methods The assay uses decellularized and solubilized hea...

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Veröffentlicht in:	Xenotransplantation (Københaven) 2021-01, Vol.28 (1), p.e12646-n/a
Hauptverfasser:	Smart, Isabel, Goecke, Tobias, Ramm, Robert, Petersen, Björn, Lenz, Doreen, Haverich, Axel, Niemann, Heiner, Hilfiker, Andres
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies antibody decellularization Enzyme-linked immunosorbent assay Epitopes Extracellular matrix Heart preformed antibodies solubilized ECM Transplantation xenoantigen Xenografts xenotransplantation
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