Bioprocess Technologies that Preserve the Quality of iPSCs
Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing st...
Gespeichert in:
| Veröffentlicht in: | Trends in biotechnology (Regular ed.) 2020-10, Vol.38 (10), p.1128-1140 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1140 |
|---|---|
| container_issue | 10 |
| container_start_page | 1128 |
| container_title | Trends in biotechnology (Regular ed.) |
| container_volume | 38 |
| creator | Polanco, Ashli Kuang, Bingyu Yoon, Seongkyu |
| description | Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods that measure and monitor intrinsic markers of cell differentiation state, developmental status, and viability in real time. In addition, proper cell culture modalities that nurture the growth of high-quality stem cells in suspension are critical for industrial scale-up. In this review, we present an overview of cell culture media, suspension modalities, and monitoring techniques that preserve the quality and pluripotency of iPSCs during initiation, expansion, and manufacturing.
Maintenance of pluripotent status and self-renewal capability are essential for the production of clinical-grade iPSCs for cell therapies.Media and matrix formulations and matrices have evolved through consideration of the signaling pathways that help to sustain pluripotency in iPSC lines and overall process scalability.Matrix- and feeder-free iPSC suspension culture systems overcome the limited scalability of static matrices while supporting iPSC growth and pluripotent status.Development of iPSC monitoring techniques, in silico models, and quality-by-design strategies that incorporate real-time data would enable robust process scalability. |
| doi_str_mv | 10.1016/j.tibtech.2020.03.006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2444378789</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0167779920300664</els_id><sourcerecordid>2444378789</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-d86daf4820c95c635b943d05b8d77d03c66341c1039c8fdb2af20f435d5a3fac3</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhoMoWKs_QVjw4mXXySa72XgRLX5BwYr1HNJk1qZsG022hf57U9qTF0_DwPO-zDyEXFIoKND6ZlH0btajmRcllFAAKwDqIzKgjZA5A1kfk0HiRC6ElKfkLMYFADAh6YDcPjj_HbzBGLNpqlj5zn85jFk_1302CRgxbDBtmL2vdef6bebbzE0-RvGcnLS6i3hxmEPy-fQ4Hb3k47fn19H9ODdMQJ_bpra65U0JRlamZtVMcmahmjVWCAvM1DXj1FBg0jStnZW6LaHlrLKVZq02bEiu973pzp81xl4tXTTYdXqFfh1VyTlnohGNTOjVH3Th12GVrttRjEugvElUtadM8DEGbNV3cEsdtoqC2hlVC3UwqnZGFTCVjKbc3T6H6duNw6CicbgyaF1A0yvr3T8NvzibgFE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2443490148</pqid></control><display><type>article</type><title>Bioprocess Technologies that Preserve the Quality of iPSCs</title><source>ScienceDirect Journals (5 years ago - present)</source><source>ProQuest Central UK/Ireland</source><creator>Polanco, Ashli ; Kuang, Bingyu ; Yoon, Seongkyu</creator><creatorcontrib>Polanco, Ashli ; Kuang, Bingyu ; Yoon, Seongkyu</creatorcontrib><description>Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods that measure and monitor intrinsic markers of cell differentiation state, developmental status, and viability in real time. In addition, proper cell culture modalities that nurture the growth of high-quality stem cells in suspension are critical for industrial scale-up. In this review, we present an overview of cell culture media, suspension modalities, and monitoring techniques that preserve the quality and pluripotency of iPSCs during initiation, expansion, and manufacturing.
Maintenance of pluripotent status and self-renewal capability are essential for the production of clinical-grade iPSCs for cell therapies.Media and matrix formulations and matrices have evolved through consideration of the signaling pathways that help to sustain pluripotency in iPSC lines and overall process scalability.Matrix- and feeder-free iPSC suspension culture systems overcome the limited scalability of static matrices while supporting iPSC growth and pluripotent status.Development of iPSC monitoring techniques, in silico models, and quality-by-design strategies that incorporate real-time data would enable robust process scalability.</description><identifier>ISSN: 0167-7799</identifier><identifier>EISSN: 1879-3096</identifier><identifier>DOI: 10.1016/j.tibtech.2020.03.006</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Antibodies ; Bioprocessing ; Cell adhesion & migration ; Cell culture ; Cell differentiation ; Cell growth ; cell therapy ; clinical manufacturing ; Clinical trials ; Cloning ; Culture media ; Differentiation (biology) ; Gene expression ; Good Manufacturing Practice ; Growth factors ; induced pluripotent stem cells ; Kinases ; Manufacturing ; Measurement methods ; Metabolism ; Morphology ; Pluripotency ; Proteins ; quality control ; R&D ; Research & development ; Signal transduction ; Stem cells</subject><ispartof>Trends in biotechnology (Regular ed.), 2020-10, Vol.38 (10), p.1128-1140</ispartof><rights>2020 Elsevier Ltd</rights><rights>2020. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-d86daf4820c95c635b943d05b8d77d03c66341c1039c8fdb2af20f435d5a3fac3</citedby><cites>FETCH-LOGICAL-c370t-d86daf4820c95c635b943d05b8d77d03c66341c1039c8fdb2af20f435d5a3fac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2443490148?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994,64384,64386,64388,72240</link.rule.ids></links><search><creatorcontrib>Polanco, Ashli</creatorcontrib><creatorcontrib>Kuang, Bingyu</creatorcontrib><creatorcontrib>Yoon, Seongkyu</creatorcontrib><title>Bioprocess Technologies that Preserve the Quality of iPSCs</title><title>Trends in biotechnology (Regular ed.)</title><description>Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods that measure and monitor intrinsic markers of cell differentiation state, developmental status, and viability in real time. In addition, proper cell culture modalities that nurture the growth of high-quality stem cells in suspension are critical for industrial scale-up. In this review, we present an overview of cell culture media, suspension modalities, and monitoring techniques that preserve the quality and pluripotency of iPSCs during initiation, expansion, and manufacturing.
Maintenance of pluripotent status and self-renewal capability are essential for the production of clinical-grade iPSCs for cell therapies.Media and matrix formulations and matrices have evolved through consideration of the signaling pathways that help to sustain pluripotency in iPSC lines and overall process scalability.Matrix- and feeder-free iPSC suspension culture systems overcome the limited scalability of static matrices while supporting iPSC growth and pluripotent status.Development of iPSC monitoring techniques, in silico models, and quality-by-design strategies that incorporate real-time data would enable robust process scalability.</description><subject>Antibodies</subject><subject>Bioprocessing</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell growth</subject><subject>cell therapy</subject><subject>clinical manufacturing</subject><subject>Clinical trials</subject><subject>Cloning</subject><subject>Culture media</subject><subject>Differentiation (biology)</subject><subject>Gene expression</subject><subject>Good Manufacturing Practice</subject><subject>Growth factors</subject><subject>induced pluripotent stem cells</subject><subject>Kinases</subject><subject>Manufacturing</subject><subject>Measurement methods</subject><subject>Metabolism</subject><subject>Morphology</subject><subject>Pluripotency</subject><subject>Proteins</subject><subject>quality control</subject><subject>R&D</subject><subject>Research & development</subject><subject>Signal transduction</subject><subject>Stem cells</subject><issn>0167-7799</issn><issn>1879-3096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkE1LAzEQhoMoWKs_QVjw4mXXySa72XgRLX5BwYr1HNJk1qZsG022hf57U9qTF0_DwPO-zDyEXFIoKND6ZlH0btajmRcllFAAKwDqIzKgjZA5A1kfk0HiRC6ElKfkLMYFADAh6YDcPjj_HbzBGLNpqlj5zn85jFk_1302CRgxbDBtmL2vdef6bebbzE0-RvGcnLS6i3hxmEPy-fQ4Hb3k47fn19H9ODdMQJ_bpra65U0JRlamZtVMcmahmjVWCAvM1DXj1FBg0jStnZW6LaHlrLKVZq02bEiu973pzp81xl4tXTTYdXqFfh1VyTlnohGNTOjVH3Th12GVrttRjEugvElUtadM8DEGbNV3cEsdtoqC2hlVC3UwqnZGFTCVjKbc3T6H6duNw6CicbgyaF1A0yvr3T8NvzibgFE</recordid><startdate>202010</startdate><enddate>202010</enddate><creator>Polanco, Ashli</creator><creator>Kuang, Bingyu</creator><creator>Yoon, Seongkyu</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>202010</creationdate><title>Bioprocess Technologies that Preserve the Quality of iPSCs</title><author>Polanco, Ashli ; Kuang, Bingyu ; Yoon, Seongkyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-d86daf4820c95c635b943d05b8d77d03c66341c1039c8fdb2af20f435d5a3fac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antibodies</topic><topic>Bioprocessing</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell growth</topic><topic>cell therapy</topic><topic>clinical manufacturing</topic><topic>Clinical trials</topic><topic>Cloning</topic><topic>Culture media</topic><topic>Differentiation (biology)</topic><topic>Gene expression</topic><topic>Good Manufacturing Practice</topic><topic>Growth factors</topic><topic>induced pluripotent stem cells</topic><topic>Kinases</topic><topic>Manufacturing</topic><topic>Measurement methods</topic><topic>Metabolism</topic><topic>Morphology</topic><topic>Pluripotency</topic><topic>Proteins</topic><topic>quality control</topic><topic>R&D</topic><topic>Research & development</topic><topic>Signal transduction</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polanco, Ashli</creatorcontrib><creatorcontrib>Kuang, Bingyu</creatorcontrib><creatorcontrib>Yoon, Seongkyu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Trends in biotechnology (Regular ed.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polanco, Ashli</au><au>Kuang, Bingyu</au><au>Yoon, Seongkyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioprocess Technologies that Preserve the Quality of iPSCs</atitle><jtitle>Trends in biotechnology (Regular ed.)</jtitle><date>2020-10</date><risdate>2020</risdate><volume>38</volume><issue>10</issue><spage>1128</spage><epage>1140</epage><pages>1128-1140</pages><issn>0167-7799</issn><eissn>1879-3096</eissn><abstract>Large-scale production of induced pluripotent stem cells (iPSCs) is essential for the treatment of a variety of clinical indications. However, culturing enough iPSCs for clinical applications is problematic due to their sensitive pluripotent state and dependence on a supporting matrix. Developing stem cell bioprocessing strategies that are scalable and meet clinical needs requires incorporating methods that measure and monitor intrinsic markers of cell differentiation state, developmental status, and viability in real time. In addition, proper cell culture modalities that nurture the growth of high-quality stem cells in suspension are critical for industrial scale-up. In this review, we present an overview of cell culture media, suspension modalities, and monitoring techniques that preserve the quality and pluripotency of iPSCs during initiation, expansion, and manufacturing.
Maintenance of pluripotent status and self-renewal capability are essential for the production of clinical-grade iPSCs for cell therapies.Media and matrix formulations and matrices have evolved through consideration of the signaling pathways that help to sustain pluripotency in iPSC lines and overall process scalability.Matrix- and feeder-free iPSC suspension culture systems overcome the limited scalability of static matrices while supporting iPSC growth and pluripotent status.Development of iPSC monitoring techniques, in silico models, and quality-by-design strategies that incorporate real-time data would enable robust process scalability.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.tibtech.2020.03.006</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7799 |
| ispartof | Trends in biotechnology (Regular ed.), 2020-10, Vol.38 (10), p.1128-1140 |
| issn | 0167-7799 1879-3096 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2444378789 |
| source | ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Antibodies Bioprocessing Cell adhesion & migration Cell culture Cell differentiation Cell growth cell therapy clinical manufacturing Clinical trials Cloning Culture media Differentiation (biology) Gene expression Good Manufacturing Practice Growth factors induced pluripotent stem cells Kinases Manufacturing Measurement methods Metabolism Morphology Pluripotency Proteins quality control R&D Research & development Signal transduction Stem cells |
| title | Bioprocess Technologies that Preserve the Quality of iPSCs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T13%3A42%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bioprocess%20Technologies%20that%20Preserve%20the%20Quality%20of%20iPSCs&rft.jtitle=Trends%20in%20biotechnology%20(Regular%20ed.)&rft.au=Polanco,%20Ashli&rft.date=2020-10&rft.volume=38&rft.issue=10&rft.spage=1128&rft.epage=1140&rft.pages=1128-1140&rft.issn=0167-7799&rft.eissn=1879-3096&rft_id=info:doi/10.1016/j.tibtech.2020.03.006&rft_dat=%3Cproquest_cross%3E2444378789%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2443490148&rft_id=info:pmid/&rft_els_id=S0167779920300664&rfr_iscdi=true |