Correlation between clinical outcome and tissue inflammatory response in kidney transplant recipients with cryptococcosis
ABSTRACT Cryptococcosis is the second most common invasive fungal infection reported in renal transplant recipients. Tissue granulomatous inflammation is necessary to contain Cryptococcus infection. This study aims to analyze the granuloma patterns and in situ expression of regulatory T (Treg) immun...
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| Veröffentlicht in: | Pathogens and Disease 2020-10, Vol.78 (7), p.1 |
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| creator | Nishikaku, Angela S Soldá, Marcel V Ricci, Giannina Ponzio, Vinicius Pagliari, Carla Medina-Pestana, José O de Franco, Marcello F Colombo, Arnaldo Lopes |
| description | ABSTRACT
Cryptococcosis is the second most common invasive fungal infection reported in renal transplant recipients. Tissue granulomatous inflammation is necessary to contain Cryptococcus infection. This study aims to analyze the granuloma patterns and in situ expression of regulatory T (Treg) immune response in tissue samples from 12 renal transplant recipients with cryptococcosis. Fungal isolates were molecularly identified as Cryptococcus neoformans species complex. A detailed characterization of granulomas in tissue samples from 12 kidney transplant recipients with cryptococcosis was described by checking six lung and six skin biopsies by conventional histology and for immunohistochemical detection of CD4 and Treg markers: forkhead box P3 (FoxP3), interleukin (IL)-10 and transforming-growth factor (TGF)-β. Granulomas were classified as compact, loose or mixed. Patients with mixed (n = 4) and compact (n = 3) granulomatous inflammation patterns were associated with a better prognosis and presented a higher number of CD4+FoxP3+T cells compared to the group of patients with loose granulomas. In counterpart, three out of five patients with loose granulomas died with cryptococcosis. We suggest that Treg may have a protective role in the tissue response to Cryptococcus infection given its association with compact and mixed granulomas in patients with better clinical outcomes.
Tissue inflammatory host response to Cryptococcus spp. may predict outcome in kidney transplant recipients with cryptococcosis. |
| doi_str_mv | 10.1093/femspd/ftaa054 |
| format | Article |
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Cryptococcosis is the second most common invasive fungal infection reported in renal transplant recipients. Tissue granulomatous inflammation is necessary to contain Cryptococcus infection. This study aims to analyze the granuloma patterns and in situ expression of regulatory T (Treg) immune response in tissue samples from 12 renal transplant recipients with cryptococcosis. Fungal isolates were molecularly identified as Cryptococcus neoformans species complex. A detailed characterization of granulomas in tissue samples from 12 kidney transplant recipients with cryptococcosis was described by checking six lung and six skin biopsies by conventional histology and for immunohistochemical detection of CD4 and Treg markers: forkhead box P3 (FoxP3), interleukin (IL)-10 and transforming-growth factor (TGF)-β. Granulomas were classified as compact, loose or mixed. Patients with mixed (n = 4) and compact (n = 3) granulomatous inflammation patterns were associated with a better prognosis and presented a higher number of CD4+FoxP3+T cells compared to the group of patients with loose granulomas. In counterpart, three out of five patients with loose granulomas died with cryptococcosis. We suggest that Treg may have a protective role in the tissue response to Cryptococcus infection given its association with compact and mixed granulomas in patients with better clinical outcomes.
Tissue inflammatory host response to Cryptococcus spp. may predict outcome in kidney transplant recipients with cryptococcosis.</description><identifier>ISSN: 2049-632X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1093/femspd/ftaa054</identifier><identifier>PMID: 32945853</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>CD4 antigen ; Clinical outcomes ; Cryptococcal infections ; Cryptococcosis ; Cryptococcus ; Demographic aspects ; Forkhead protein ; Foxp3 protein ; Fungal infections ; Fungi ; Granuloma ; Granulomas ; Growth factors ; Histology ; Immune response ; Immune system ; Infections ; Inflammation ; Inflammatory response ; Interleukins ; Kidney transplantation ; Kidney transplants ; Kidneys ; Lymphocytes ; Lymphocytes T ; Medical examination ; Organ transplant recipients ; Patient outcomes ; Risk factors ; Tissues ; Transplantation</subject><ispartof>Pathogens and Disease, 2020-10, Vol.78 (7), p.1</ispartof><rights>The Author(s) 2020. Published by Oxford University Press on behalf of FEMS. 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.</rights><rights>COPYRIGHT 2020 Oxford University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-5fda98519524b3a0e324dc257b063a2962d21035222e40dd06ccc97ecc718fcf3</citedby><cites>FETCH-LOGICAL-c424t-5fda98519524b3a0e324dc257b063a2962d21035222e40dd06ccc97ecc718fcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1598,27901,27902</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/femspd/ftaa054$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32945853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishikaku, Angela S</creatorcontrib><creatorcontrib>Soldá, Marcel V</creatorcontrib><creatorcontrib>Ricci, Giannina</creatorcontrib><creatorcontrib>Ponzio, Vinicius</creatorcontrib><creatorcontrib>Pagliari, Carla</creatorcontrib><creatorcontrib>Medina-Pestana, José O</creatorcontrib><creatorcontrib>de Franco, Marcello F</creatorcontrib><creatorcontrib>Colombo, Arnaldo Lopes</creatorcontrib><title>Correlation between clinical outcome and tissue inflammatory response in kidney transplant recipients with cryptococcosis</title><title>Pathogens and Disease</title><addtitle>Pathog Dis</addtitle><description>ABSTRACT
Cryptococcosis is the second most common invasive fungal infection reported in renal transplant recipients. Tissue granulomatous inflammation is necessary to contain Cryptococcus infection. This study aims to analyze the granuloma patterns and in situ expression of regulatory T (Treg) immune response in tissue samples from 12 renal transplant recipients with cryptococcosis. Fungal isolates were molecularly identified as Cryptococcus neoformans species complex. A detailed characterization of granulomas in tissue samples from 12 kidney transplant recipients with cryptococcosis was described by checking six lung and six skin biopsies by conventional histology and for immunohistochemical detection of CD4 and Treg markers: forkhead box P3 (FoxP3), interleukin (IL)-10 and transforming-growth factor (TGF)-β. Granulomas were classified as compact, loose or mixed. Patients with mixed (n = 4) and compact (n = 3) granulomatous inflammation patterns were associated with a better prognosis and presented a higher number of CD4+FoxP3+T cells compared to the group of patients with loose granulomas. In counterpart, three out of five patients with loose granulomas died with cryptococcosis. We suggest that Treg may have a protective role in the tissue response to Cryptococcus infection given its association with compact and mixed granulomas in patients with better clinical outcomes.
Tissue inflammatory host response to Cryptococcus spp. may predict outcome in kidney transplant recipients with cryptococcosis.</description><subject>CD4 antigen</subject><subject>Clinical outcomes</subject><subject>Cryptococcal infections</subject><subject>Cryptococcosis</subject><subject>Cryptococcus</subject><subject>Demographic aspects</subject><subject>Forkhead protein</subject><subject>Foxp3 protein</subject><subject>Fungal infections</subject><subject>Fungi</subject><subject>Granuloma</subject><subject>Granulomas</subject><subject>Growth factors</subject><subject>Histology</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukins</subject><subject>Kidney transplantation</subject><subject>Kidney transplants</subject><subject>Kidneys</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medical examination</subject><subject>Organ transplant recipients</subject><subject>Patient outcomes</subject><subject>Risk factors</subject><subject>Tissues</subject><subject>Transplantation</subject><issn>2049-632X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqFkcuLFDEQxoMo7rLu1aMEvOhhdvPq13EZfMGCFwVvTaZS0azdSZtKs_R_b5YZH3gxOaSo-lXxpT7GnktxJcWgrz3OtLhrX6wVjXnEzpUww67V6svjv-Izdkl0J-rpG9l37VN2ptVgmr7R52zbp5xxsiWkyA9Y7hEjhynEAHbiaS2QZuQ2Ol4C0Yo8RD_ZebYl5Y1npCVFesjy78FF3HjJNtIy2VhqFcISMBbi96F845C3pSRIAIkCPWNPvJ0IL0_vBfv89s2n_fvd7cd3H_Y3tzswypRd450dqvChUeagrUCtjAPVdAfRaquGVjklhW6UUmiEc6IFgKFDgE72Hry-YK-Oc5ecfqxIZZwDAU5VIqaVRmWM0V1npK7oy3_Qu7TmWNVVqmtl2_VKVOrqSH21E451H6n-Gep1OAdIEX2o-Zt2EMZIpcyfBsiJKKMflxxmm7dRivHByPFo5Hgysja8OOlYDzO63_gv2yrw-gikdfnfsJ__sKv2</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Nishikaku, Angela S</creator><creator>Soldá, Marcel V</creator><creator>Ricci, Giannina</creator><creator>Ponzio, Vinicius</creator><creator>Pagliari, Carla</creator><creator>Medina-Pestana, José O</creator><creator>de Franco, Marcello F</creator><creator>Colombo, Arnaldo Lopes</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>3V.</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20201001</creationdate><title>Correlation between clinical outcome and tissue inflammatory response in kidney transplant recipients with cryptococcosis</title><author>Nishikaku, Angela S ; 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Cryptococcosis is the second most common invasive fungal infection reported in renal transplant recipients. Tissue granulomatous inflammation is necessary to contain Cryptococcus infection. This study aims to analyze the granuloma patterns and in situ expression of regulatory T (Treg) immune response in tissue samples from 12 renal transplant recipients with cryptococcosis. Fungal isolates were molecularly identified as Cryptococcus neoformans species complex. A detailed characterization of granulomas in tissue samples from 12 kidney transplant recipients with cryptococcosis was described by checking six lung and six skin biopsies by conventional histology and for immunohistochemical detection of CD4 and Treg markers: forkhead box P3 (FoxP3), interleukin (IL)-10 and transforming-growth factor (TGF)-β. Granulomas were classified as compact, loose or mixed. Patients with mixed (n = 4) and compact (n = 3) granulomatous inflammation patterns were associated with a better prognosis and presented a higher number of CD4+FoxP3+T cells compared to the group of patients with loose granulomas. In counterpart, three out of five patients with loose granulomas died with cryptococcosis. We suggest that Treg may have a protective role in the tissue response to Cryptococcus infection given its association with compact and mixed granulomas in patients with better clinical outcomes.
Tissue inflammatory host response to Cryptococcus spp. may predict outcome in kidney transplant recipients with cryptococcosis.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>32945853</pmid><doi>10.1093/femspd/ftaa054</doi></addata></record> |
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| subjects | CD4 antigen Clinical outcomes Cryptococcal infections Cryptococcosis Cryptococcus Demographic aspects Forkhead protein Foxp3 protein Fungal infections Fungi Granuloma Granulomas Growth factors Histology Immune response Immune system Infections Inflammation Inflammatory response Interleukins Kidney transplantation Kidney transplants Kidneys Lymphocytes Lymphocytes T Medical examination Organ transplant recipients Patient outcomes Risk factors Tissues Transplantation |
| title | Correlation between clinical outcome and tissue inflammatory response in kidney transplant recipients with cryptococcosis |
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