Application of Metabolomics Tools to Determine Possible Biomarker Metabolites Linked to Leaf Blackening in Protea
The postharvesting disorder leaf blackening is the main cause of product rejection in Protea during export. In this study, we report an investigation into metabolites associated with leaf blackening in Protea species. Methanol extracts of leaf and involucral bract tissue were analyzed by liquid chro...
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Veröffentlicht in: | Journal of agricultural and food chemistry 2020-11, Vol.68 (45), p.12595-12605 |
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creator | Masike, Keabetswe de Villiers, André Hoffman, Eleanor W Stander, Maria A |
description | The postharvesting disorder leaf blackening is the main cause of product rejection in Protea during export. In this study, we report an investigation into metabolites associated with leaf blackening in Protea species. Methanol extracts of leaf and involucral bract tissue were analyzed by liquid chromatography hyphenated to photodiode array and high-resolution mass spectrometry (LC–PDA–HRMS), where 116 features were annotated. Analytical data obtained from 37 Protea species, selections, and hybrids were investigated using metabolomics tools, which showed that stems susceptible to leaf blackening cluster together and contained features identified as benzenetriol- and/or hydroquinone-derived metabolites. On the other hand, species, selections, and cultivars not prone to blackening were linked to metabolites with known protective properties against biotic and abiotic stressors. During the browning process, susceptible cultivars also produce these protective metabolites, yet at innately low levels, which may render these species and cultivars more vulnerable to blackening. Metabolites that were found to be correlated to the instigation of the browning process, all comprising benzenetriol- and hydroquinone-glycoside derivatives, are highlighted to provide preliminary insights to guide the development of new Protea cultivars not susceptible to leaf blackening. |
doi_str_mv | 10.1021/acs.jafc.0c03607 |
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Metabolites that were found to be correlated to the instigation of the browning process, all comprising benzenetriol- and hydroquinone-glycoside derivatives, are highlighted to provide preliminary insights to guide the development of new Protea cultivars not susceptible to leaf blackening.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.0c03607</identifier><identifier>PMID: 32936621</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bioactive Constituents, Metabolites, and Functions ; Biomarkers - chemistry ; Color ; Metabolomics ; Plant Extracts - chemistry ; Plant Extracts - metabolism ; Plant Leaves - chemistry ; Plant Leaves - metabolism ; Proteaceae - chemistry ; Proteaceae - growth & development ; Proteaceae - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2020-11, Vol.68 (45), p.12595-12605</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-8421bc1ed145ade7c83fa0448b060680fc04a4e65feb818894210f478b6e071a3</citedby><cites>FETCH-LOGICAL-a336t-8421bc1ed145ade7c83fa0448b060680fc04a4e65feb818894210f478b6e071a3</cites><orcidid>0000-0003-0806-9734</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.0c03607$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.0c03607$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32936621$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masike, Keabetswe</creatorcontrib><creatorcontrib>de Villiers, André</creatorcontrib><creatorcontrib>Hoffman, Eleanor W</creatorcontrib><creatorcontrib>Stander, Maria A</creatorcontrib><title>Application of Metabolomics Tools to Determine Possible Biomarker Metabolites Linked to Leaf Blackening in Protea</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The postharvesting disorder leaf blackening is the main cause of product rejection in Protea during export. In this study, we report an investigation into metabolites associated with leaf blackening in Protea species. Methanol extracts of leaf and involucral bract tissue were analyzed by liquid chromatography hyphenated to photodiode array and high-resolution mass spectrometry (LC–PDA–HRMS), where 116 features were annotated. Analytical data obtained from 37 Protea species, selections, and hybrids were investigated using metabolomics tools, which showed that stems susceptible to leaf blackening cluster together and contained features identified as benzenetriol- and/or hydroquinone-derived metabolites. On the other hand, species, selections, and cultivars not prone to blackening were linked to metabolites with known protective properties against biotic and abiotic stressors. During the browning process, susceptible cultivars also produce these protective metabolites, yet at innately low levels, which may render these species and cultivars more vulnerable to blackening. Metabolites that were found to be correlated to the instigation of the browning process, all comprising benzenetriol- and hydroquinone-glycoside derivatives, are highlighted to provide preliminary insights to guide the development of new Protea cultivars not susceptible to leaf blackening.</description><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Biomarkers - chemistry</subject><subject>Color</subject><subject>Metabolomics</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Leaves - metabolism</subject><subject>Proteaceae - chemistry</subject><subject>Proteaceae - growth & development</subject><subject>Proteaceae - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL1PwzAQRy0EoqWwMyGPDKScE8dxx7Z8SkV0KHPkuGfkNolbOx3473FpYWO65b2fdI-QawZDBim7VzoMV8roIWjIBBQnpM_yFJKcMXlK-hCZROaC9chFCCsAkHkB56SXpaNMiJT1yXa82dRWq866ljpD37BTlatdY3WgC-fqQDtHH7BD39gW6dyFYKsa6cS6Rvk1-l_FdhjozLZrXO6VGSpDJ7XSa2xt-0ltS-fedaguyZlRdcCr4x2Qj6fHxfQlmb0_v07Hs0RlmegSyVNWaYZLxnO1xELLzCjgXFYgQEgwGrjiKHKDlWRSjiIPhheyEggFU9mA3B52N95tdxi6srFBY12rFt0ulCnnmZRslPKIwgHVPr7n0ZQbb-N3XyWDch-6jKHLfejyGDoqN8f1XdXg8k_4LRuBuwPwo7qdb-Oz_-99A5mfifo</recordid><startdate>20201111</startdate><enddate>20201111</enddate><creator>Masike, Keabetswe</creator><creator>de Villiers, André</creator><creator>Hoffman, Eleanor W</creator><creator>Stander, Maria A</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0806-9734</orcidid></search><sort><creationdate>20201111</creationdate><title>Application of Metabolomics Tools to Determine Possible Biomarker Metabolites Linked to Leaf Blackening in Protea</title><author>Masike, Keabetswe ; de Villiers, André ; Hoffman, Eleanor W ; Stander, Maria A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-8421bc1ed145ade7c83fa0448b060680fc04a4e65feb818894210f478b6e071a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Biomarkers - chemistry</topic><topic>Color</topic><topic>Metabolomics</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Leaves - metabolism</topic><topic>Proteaceae - chemistry</topic><topic>Proteaceae - growth & development</topic><topic>Proteaceae - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masike, Keabetswe</creatorcontrib><creatorcontrib>de Villiers, André</creatorcontrib><creatorcontrib>Hoffman, Eleanor W</creatorcontrib><creatorcontrib>Stander, Maria A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masike, Keabetswe</au><au>de Villiers, André</au><au>Hoffman, Eleanor W</au><au>Stander, Maria A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Metabolomics Tools to Determine Possible Biomarker Metabolites Linked to Leaf Blackening in Protea</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2020-11-11</date><risdate>2020</risdate><volume>68</volume><issue>45</issue><spage>12595</spage><epage>12605</epage><pages>12595-12605</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>The postharvesting disorder leaf blackening is the main cause of product rejection in Protea during export. In this study, we report an investigation into metabolites associated with leaf blackening in Protea species. Methanol extracts of leaf and involucral bract tissue were analyzed by liquid chromatography hyphenated to photodiode array and high-resolution mass spectrometry (LC–PDA–HRMS), where 116 features were annotated. Analytical data obtained from 37 Protea species, selections, and hybrids were investigated using metabolomics tools, which showed that stems susceptible to leaf blackening cluster together and contained features identified as benzenetriol- and/or hydroquinone-derived metabolites. On the other hand, species, selections, and cultivars not prone to blackening were linked to metabolites with known protective properties against biotic and abiotic stressors. During the browning process, susceptible cultivars also produce these protective metabolites, yet at innately low levels, which may render these species and cultivars more vulnerable to blackening. Metabolites that were found to be correlated to the instigation of the browning process, all comprising benzenetriol- and hydroquinone-glycoside derivatives, are highlighted to provide preliminary insights to guide the development of new Protea cultivars not susceptible to leaf blackening.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>32936621</pmid><doi>10.1021/acs.jafc.0c03607</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-0806-9734</orcidid></addata></record> |
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subjects | Bioactive Constituents, Metabolites, and Functions Biomarkers - chemistry Color Metabolomics Plant Extracts - chemistry Plant Extracts - metabolism Plant Leaves - chemistry Plant Leaves - metabolism Proteaceae - chemistry Proteaceae - growth & development Proteaceae - metabolism |
title | Application of Metabolomics Tools to Determine Possible Biomarker Metabolites Linked to Leaf Blackening in Protea |
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