HPLC-MS/MS method for the determination of perfluoroalkyl substances in breast milk by combining salt-assisted and dispersive liquid-liquid microextraction
The widespread use of perfluoroalkyl substances has resulted in the universal exposure of humans to these endocrine-disrupting chemicals, including the exposure of neonates through breastfeeding. The objective of this study was to develop a method to determine 10 perfluoroalkyl substances in breast...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2020-11, Vol.412 (28), p.7913-7923 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Vela-Soria, Fernando Serrano-López, Laura García-Villanova, Javier de Haro, Tomás Olea, Nicolas Freire, Carmen |
| description | The widespread use of perfluoroalkyl substances has resulted in the universal exposure of humans to these endocrine-disrupting chemicals, including the exposure of neonates through breastfeeding. The objective of this study was to develop a method to determine 10 perfluoroalkyl substances in breast milk (1-mL aliquot) by combining salt-assisted liquid-liquid extraction with dispersive liquid-liquid microextraction and using high-performance liquid chromatography-tandem mass spectrometry. Chemometric strategies were applied to optimize experimental parameters. The limit of quantification was 20 pg mL
−1
for all analytes, and inter-day variability (evaluated as relative standard deviation) ranged from 8.2 to 13.8%. The method was validated by a recovery assay with spiked samples. Percentage recoveries ranged from 85.9 to 110.8%. The method was satisfactorily applied to assess target compounds in 20 breast milk samples from donors. Perfluorooctanoic acid, perfluorooctane sulfonate, and perfluorohexanoic acid were the most frequently detected analytes. This analytical procedure can provide useful information on newborn’s exposure to these xenobiotics. |
| doi_str_mv | 10.1007/s00216-020-02924-x |
| format | Article |
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−1
for all analytes, and inter-day variability (evaluated as relative standard deviation) ranged from 8.2 to 13.8%. The method was validated by a recovery assay with spiked samples. Percentage recoveries ranged from 85.9 to 110.8%. The method was satisfactorily applied to assess target compounds in 20 breast milk samples from donors. Perfluorooctanoic acid, perfluorooctane sulfonate, and perfluorohexanoic acid were the most frequently detected analytes. This analytical procedure can provide useful information on newborn’s exposure to these xenobiotics.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-020-02924-x</identifier><identifier>PMID: 32935152</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Ammonium perfluorooctanoate ; Analysis ; Analytical Chemistry ; Animals ; Biochemistry ; Breast feeding ; Characterization and Evaluation of Materials ; Chemicals ; Chemistry ; Chemistry and Materials Science ; Chromatography, High Pressure Liquid - methods ; Dietary Exposure ; Fluorocarbons - analysis ; Fluorocarbons - toxicity ; Food Science ; High performance liquid chromatography ; Humans ; Infant, Newborn ; Laboratory Medicine ; Limit of Detection ; Liquid Phase Microextraction - methods ; Mass spectrometry ; Methods ; Milk, Human - chemistry ; Monitoring/Environmental Analysis ; Reference Standards ; Reproducibility of Results ; Research Paper ; Salts - chemistry ; Tandem Mass Spectrometry - methods</subject><ispartof>Analytical and bioanalytical chemistry, 2020-11, Vol.412 (28), p.7913-7923</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-36c5b19a8b2c28ba57b4c8c6faa5d0319c79a96b364beb87bf68196add82d0ad3</citedby><cites>FETCH-LOGICAL-c451t-36c5b19a8b2c28ba57b4c8c6faa5d0319c79a96b364beb87bf68196add82d0ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-020-02924-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-020-02924-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32935152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vela-Soria, Fernando</creatorcontrib><creatorcontrib>Serrano-López, Laura</creatorcontrib><creatorcontrib>García-Villanova, Javier</creatorcontrib><creatorcontrib>de Haro, Tomás</creatorcontrib><creatorcontrib>Olea, Nicolas</creatorcontrib><creatorcontrib>Freire, Carmen</creatorcontrib><title>HPLC-MS/MS method for the determination of perfluoroalkyl substances in breast milk by combining salt-assisted and dispersive liquid-liquid microextraction</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The widespread use of perfluoroalkyl substances has resulted in the universal exposure of humans to these endocrine-disrupting chemicals, including the exposure of neonates through breastfeeding. The objective of this study was to develop a method to determine 10 perfluoroalkyl substances in breast milk (1-mL aliquot) by combining salt-assisted liquid-liquid extraction with dispersive liquid-liquid microextraction and using high-performance liquid chromatography-tandem mass spectrometry. Chemometric strategies were applied to optimize experimental parameters. The limit of quantification was 20 pg mL
−1
for all analytes, and inter-day variability (evaluated as relative standard deviation) ranged from 8.2 to 13.8%. The method was validated by a recovery assay with spiked samples. Percentage recoveries ranged from 85.9 to 110.8%. The method was satisfactorily applied to assess target compounds in 20 breast milk samples from donors. Perfluorooctanoic acid, perfluorooctane sulfonate, and perfluorohexanoic acid were the most frequently detected analytes. This analytical procedure can provide useful information on newborn’s exposure to these xenobiotics.</description><subject>Ammonium perfluorooctanoate</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Breast feeding</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemicals</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dietary Exposure</subject><subject>Fluorocarbons - analysis</subject><subject>Fluorocarbons - toxicity</subject><subject>Food Science</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Liquid Phase Microextraction - methods</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Milk, Human - chemistry</subject><subject>Monitoring/Environmental Analysis</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Salts - chemistry</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UV1rFDEUHUSxdfUP-CABX3yZNslMMjOPZalW2KJQfQ75uLNNm0m2SUZ2f4t_1qxTC4JICOeSnHNyb05VvSX4jGDcnSeMKeE1prjsgbb1_ll1Sjjpa8oZfv5Ut_SkepXSHcaE9YS_rE4aOjSMMHpa_bz6ulnX1zfn1zdognwbDBpDRPkWkIEMcbJeZhs8CiPaQRzdHGKQ7v7gUJpVytJrSMh6pCLIlNFk3T1SB6TDpKy3fouSdLmWKdmUwSDpDTI2FatkfwBy9mG2pl6giHUMsM9R6uObr6sXo3QJ3jziqvr-8fLb-qrefPn0eX2xqXXLSK4brpkig-wV1bRXknWq1b3mo5TM4IYMuhvkwFXDWwWq79TIezJwaUxPDZamWVUfFt9dDA8zpCwmmzQ4Jz2EOQnatg2juCuwqt4v1K10IKwfw7HZI11c8KanbKC0K6yzf7DKMlBGDB5GW87_EtBFUOZPKcIodtFOMh4EweKYtViyFiVr8TtrsS-id49tz2oC8yT5E24hNAshlSu_hSjuwhx9-cr_2f4Cglu3rg</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Vela-Soria, Fernando</creator><creator>Serrano-López, Laura</creator><creator>García-Villanova, Javier</creator><creator>de Haro, Tomás</creator><creator>Olea, Nicolas</creator><creator>Freire, Carmen</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20201101</creationdate><title>HPLC-MS/MS method for the determination of perfluoroalkyl substances in breast milk by combining salt-assisted and dispersive liquid-liquid microextraction</title><author>Vela-Soria, Fernando ; 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The objective of this study was to develop a method to determine 10 perfluoroalkyl substances in breast milk (1-mL aliquot) by combining salt-assisted liquid-liquid extraction with dispersive liquid-liquid microextraction and using high-performance liquid chromatography-tandem mass spectrometry. Chemometric strategies were applied to optimize experimental parameters. The limit of quantification was 20 pg mL
−1
for all analytes, and inter-day variability (evaluated as relative standard deviation) ranged from 8.2 to 13.8%. The method was validated by a recovery assay with spiked samples. Percentage recoveries ranged from 85.9 to 110.8%. The method was satisfactorily applied to assess target compounds in 20 breast milk samples from donors. Perfluorooctanoic acid, perfluorooctane sulfonate, and perfluorohexanoic acid were the most frequently detected analytes. This analytical procedure can provide useful information on newborn’s exposure to these xenobiotics.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32935152</pmid><doi>10.1007/s00216-020-02924-x</doi><tpages>11</tpages></addata></record> |
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| subjects | Ammonium perfluorooctanoate Analysis Analytical Chemistry Animals Biochemistry Breast feeding Characterization and Evaluation of Materials Chemicals Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid - methods Dietary Exposure Fluorocarbons - analysis Fluorocarbons - toxicity Food Science High performance liquid chromatography Humans Infant, Newborn Laboratory Medicine Limit of Detection Liquid Phase Microextraction - methods Mass spectrometry Methods Milk, Human - chemistry Monitoring/Environmental Analysis Reference Standards Reproducibility of Results Research Paper Salts - chemistry Tandem Mass Spectrometry - methods |
| title | HPLC-MS/MS method for the determination of perfluoroalkyl substances in breast milk by combining salt-assisted and dispersive liquid-liquid microextraction |
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