Anti-nociceptive effect of Arbutus andrachne L. methanolic leaf extract mediated by CB1, TRPV1 and PPARs in mouse pain models
Arbutus andrachne L. is a medicinal plant that grows in Jordan and has many valuable effects. In the present study, the anti-nociceptive effect of A. andrachne methanolic leaf extract was determined in mice using thermal and chemical tests. Our findings show that different doses of A. andrachne ext...
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Veröffentlicht in: | Inflammopharmacology 2020-12, Vol.28 (6), p.1567-1577 |
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description | Arbutus andrachne
L. is a medicinal plant that grows in Jordan and has many valuable effects. In the present study, the anti-nociceptive effect of
A. andrachne
methanolic leaf extract was determined in mice using thermal and chemical tests. Our findings show that different doses of
A. andrachne
extract reduced the number of writhings significantly compared to control group. The leaf extract also reduced the time of paw licking in the early and late phases of formalin test. In all the conducted tests, 300 mg/kg body wt. was the best effective dose. A peroxisome proliferator-activated receptor alpha (PPARα) antagonist reversed the action of the plant extract in the early phase of formalin test while antagonists of the PPARα, PPAR gamma (PPARγ) and cannabinoid 1 (CB1) receptors were responsible for abolishing its effect in the late phase of this test. Also, the extract administration increased the latency time in hot plate and tail flick, an effect that was reversed by the antagonists of PPARγ, CB1 and transient receptor potential vanilloid 1 (TRPV1). No effect was noticed for α
2
-adrenergic receptor antagonist in the action of
A. andrachne
in any of the conducted tests in this study. Furthermore, analysis of the constituents in the methanolic leaf extract using liquid chromatography mass spectrometry (LCMS) showed that the extract is rich in compounds that have anti-nociceptive and/or anti-inflammatory effects such as arbutin, rutin, linalool, linoleic acid, gallic acid, lauric acid, myristic acid, hydroquinone, β-sitosterol, ursolic acid, isoquercetin, quercetin, (+)-gallocatechin, kaempferol, α-tocopherol, myricetin 3-O-rhamnoside and catechin gallate. In conclusion,
A. andrachne
showed promising anti-nociceptive effects in thermal and chemical models of pain. These findings can open an avenue for natural pain relief. |
doi_str_mv | 10.1007/s10787-020-00746-y |
format | Article |
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L. is a medicinal plant that grows in Jordan and has many valuable effects. In the present study, the anti-nociceptive effect of
A. andrachne
methanolic leaf extract was determined in mice using thermal and chemical tests. Our findings show that different doses of
A. andrachne
extract reduced the number of writhings significantly compared to control group. The leaf extract also reduced the time of paw licking in the early and late phases of formalin test. In all the conducted tests, 300 mg/kg body wt. was the best effective dose. A peroxisome proliferator-activated receptor alpha (PPARα) antagonist reversed the action of the plant extract in the early phase of formalin test while antagonists of the PPARα, PPAR gamma (PPARγ) and cannabinoid 1 (CB1) receptors were responsible for abolishing its effect in the late phase of this test. Also, the extract administration increased the latency time in hot plate and tail flick, an effect that was reversed by the antagonists of PPARγ, CB1 and transient receptor potential vanilloid 1 (TRPV1). No effect was noticed for α
2
-adrenergic receptor antagonist in the action of
A. andrachne
in any of the conducted tests in this study. Furthermore, analysis of the constituents in the methanolic leaf extract using liquid chromatography mass spectrometry (LCMS) showed that the extract is rich in compounds that have anti-nociceptive and/or anti-inflammatory effects such as arbutin, rutin, linalool, linoleic acid, gallic acid, lauric acid, myristic acid, hydroquinone, β-sitosterol, ursolic acid, isoquercetin, quercetin, (+)-gallocatechin, kaempferol, α-tocopherol, myricetin 3-O-rhamnoside and catechin gallate. In conclusion,
A. andrachne
showed promising anti-nociceptive effects in thermal and chemical models of pain. These findings can open an avenue for natural pain relief.</description><identifier>ISSN: 0925-4692</identifier><identifier>EISSN: 1568-5608</identifier><identifier>DOI: 10.1007/s10787-020-00746-y</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Allergology ; Biomedical and Life Sciences ; Biomedicine ; Dermatology ; Gastroenterology ; Immunology ; Original Article ; Pharmacology/Toxicology ; Rheumatology</subject><ispartof>Inflammopharmacology, 2020-12, Vol.28 (6), p.1567-1577</ispartof><rights>Springer Nature Switzerland AG 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-8c0bd8e15bf46814c853fd89117107049aa8dc03143be3c2106ef5308bd20b223</citedby><cites>FETCH-LOGICAL-c324t-8c0bd8e15bf46814c853fd89117107049aa8dc03143be3c2106ef5308bd20b223</cites><orcidid>0000-0001-7115-5841</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10787-020-00746-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10787-020-00746-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Jaffal, Sahar M.</creatorcontrib><creatorcontrib>Oran, Sawsan A.</creatorcontrib><creatorcontrib>Alsalem, Mohammad</creatorcontrib><title>Anti-nociceptive effect of Arbutus andrachne L. methanolic leaf extract mediated by CB1, TRPV1 and PPARs in mouse pain models</title><title>Inflammopharmacology</title><addtitle>Inflammopharmacol</addtitle><description>Arbutus andrachne
L. is a medicinal plant that grows in Jordan and has many valuable effects. In the present study, the anti-nociceptive effect of
A. andrachne
methanolic leaf extract was determined in mice using thermal and chemical tests. Our findings show that different doses of
A. andrachne
extract reduced the number of writhings significantly compared to control group. The leaf extract also reduced the time of paw licking in the early and late phases of formalin test. In all the conducted tests, 300 mg/kg body wt. was the best effective dose. A peroxisome proliferator-activated receptor alpha (PPARα) antagonist reversed the action of the plant extract in the early phase of formalin test while antagonists of the PPARα, PPAR gamma (PPARγ) and cannabinoid 1 (CB1) receptors were responsible for abolishing its effect in the late phase of this test. Also, the extract administration increased the latency time in hot plate and tail flick, an effect that was reversed by the antagonists of PPARγ, CB1 and transient receptor potential vanilloid 1 (TRPV1). No effect was noticed for α
2
-adrenergic receptor antagonist in the action of
A. andrachne
in any of the conducted tests in this study. Furthermore, analysis of the constituents in the methanolic leaf extract using liquid chromatography mass spectrometry (LCMS) showed that the extract is rich in compounds that have anti-nociceptive and/or anti-inflammatory effects such as arbutin, rutin, linalool, linoleic acid, gallic acid, lauric acid, myristic acid, hydroquinone, β-sitosterol, ursolic acid, isoquercetin, quercetin, (+)-gallocatechin, kaempferol, α-tocopherol, myricetin 3-O-rhamnoside and catechin gallate. In conclusion,
A. andrachne
showed promising anti-nociceptive effects in thermal and chemical models of pain. These findings can open an avenue for natural pain relief.</description><subject>Allergology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Dermatology</subject><subject>Gastroenterology</subject><subject>Immunology</subject><subject>Original Article</subject><subject>Pharmacology/Toxicology</subject><subject>Rheumatology</subject><issn>0925-4692</issn><issn>1568-5608</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEtuFDEQhi0EEkPCBVh5yQKH8qPd7uUw4hFpJEZRkq3ldpdJRz3uwXajzIaz5CycDCfDmlW9_q9U9RPyjsMFB2g_Zg6taRkIYLVUmh1fkBVvtGGNBvOSrKATDVO6E6_Jm5zvAUC3uluR3-tYRhZnP3o8lPEXUgwBfaFzoOvUL2XJ1MUhOX8X8c_j9oLusdy5OE-jpxO6QPGh1Gmp_WF0BQfaH-nmE_9Ar692t_wJprvd-irTMdL9vGSkB_ecDjjlc_IquCnj23_xjNx8-Xy9-ca2379ebtZb5qVQhRkP_WCQN31Q2nDlTSPDYDrO2_o4qM45M3iQXMkepRccNIZGgukHAb0Q8oy8P-09pPnngrnY_Zg9TpOLWG-yQinZcA28qVJxkvo055ww2EMa9y4dLQf7ZLY9mW2r2fbZbHuskDxBuYrjD0z2fl5SrC_9j_oLNHWCow</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Jaffal, Sahar M.</creator><creator>Oran, Sawsan A.</creator><creator>Alsalem, Mohammad</creator><general>Springer International Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7115-5841</orcidid></search><sort><creationdate>20201201</creationdate><title>Anti-nociceptive effect of Arbutus andrachne L. methanolic leaf extract mediated by CB1, TRPV1 and PPARs in mouse pain models</title><author>Jaffal, Sahar M. ; Oran, Sawsan A. ; Alsalem, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-8c0bd8e15bf46814c853fd89117107049aa8dc03143be3c2106ef5308bd20b223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Allergology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Dermatology</topic><topic>Gastroenterology</topic><topic>Immunology</topic><topic>Original Article</topic><topic>Pharmacology/Toxicology</topic><topic>Rheumatology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jaffal, Sahar M.</creatorcontrib><creatorcontrib>Oran, Sawsan A.</creatorcontrib><creatorcontrib>Alsalem, Mohammad</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Inflammopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jaffal, Sahar M.</au><au>Oran, Sawsan A.</au><au>Alsalem, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-nociceptive effect of Arbutus andrachne L. methanolic leaf extract mediated by CB1, TRPV1 and PPARs in mouse pain models</atitle><jtitle>Inflammopharmacology</jtitle><stitle>Inflammopharmacol</stitle><date>2020-12-01</date><risdate>2020</risdate><volume>28</volume><issue>6</issue><spage>1567</spage><epage>1577</epage><pages>1567-1577</pages><issn>0925-4692</issn><eissn>1568-5608</eissn><abstract>Arbutus andrachne
L. is a medicinal plant that grows in Jordan and has many valuable effects. In the present study, the anti-nociceptive effect of
A. andrachne
methanolic leaf extract was determined in mice using thermal and chemical tests. Our findings show that different doses of
A. andrachne
extract reduced the number of writhings significantly compared to control group. The leaf extract also reduced the time of paw licking in the early and late phases of formalin test. In all the conducted tests, 300 mg/kg body wt. was the best effective dose. A peroxisome proliferator-activated receptor alpha (PPARα) antagonist reversed the action of the plant extract in the early phase of formalin test while antagonists of the PPARα, PPAR gamma (PPARγ) and cannabinoid 1 (CB1) receptors were responsible for abolishing its effect in the late phase of this test. Also, the extract administration increased the latency time in hot plate and tail flick, an effect that was reversed by the antagonists of PPARγ, CB1 and transient receptor potential vanilloid 1 (TRPV1). No effect was noticed for α
2
-adrenergic receptor antagonist in the action of
A. andrachne
in any of the conducted tests in this study. Furthermore, analysis of the constituents in the methanolic leaf extract using liquid chromatography mass spectrometry (LCMS) showed that the extract is rich in compounds that have anti-nociceptive and/or anti-inflammatory effects such as arbutin, rutin, linalool, linoleic acid, gallic acid, lauric acid, myristic acid, hydroquinone, β-sitosterol, ursolic acid, isoquercetin, quercetin, (+)-gallocatechin, kaempferol, α-tocopherol, myricetin 3-O-rhamnoside and catechin gallate. In conclusion,
A. andrachne
showed promising anti-nociceptive effects in thermal and chemical models of pain. These findings can open an avenue for natural pain relief.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s10787-020-00746-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-7115-5841</orcidid></addata></record> |
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subjects | Allergology Biomedical and Life Sciences Biomedicine Dermatology Gastroenterology Immunology Original Article Pharmacology/Toxicology Rheumatology |
title | Anti-nociceptive effect of Arbutus andrachne L. methanolic leaf extract mediated by CB1, TRPV1 and PPARs in mouse pain models |
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