Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles
A new ultrasensitive immunosensor is proposed based on the covalently attached anti-protein A antibody (IgY) on deposited gold nanoparticle (AuNP)-modified glassy carbon electrode (GCE) for the electrochemical measurement of Staphylococcus aureus ( S. aureus ) . Chicken IgY as a capture antibody pro...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2020-10, Vol.187 (10), p.567-567, Article 567 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Roushani, Mahmoud Rahmati, Zeinab Golchin, Mehdi Lotfi, Zinat Nemati, Mostafa |
| description | A new ultrasensitive immunosensor is proposed based on the covalently attached anti-protein A antibody (IgY) on deposited gold nanoparticle (AuNP)-modified glassy carbon electrode (GCE) for the electrochemical measurement of
Staphylococcus aureus
(
S. aureus
)
.
Chicken IgY as a capture antibody provides highly selective and specific binding to the target bacteria and selectively captures the
S. aureus
in its three-dimensional space. Due to that it can eliminate the interference from protein G-producing Streptococcus. In addition, the electron-transfer characteristic of [Fe(CN)
6
]
4−/3−
is hindered by this combination; as it is reflected on the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) curves. The proposed immunosensor displays a wide linear dynamic range from 10 to 10
7
CFU mL
−1
with a detection limit of 3.3 CFU mL
−1
with RSD 3.0%. It is capable to accurately determine
S. aureus
in milk and human blood serum as a complex matrix sample with satisfactory recovery of ∼ 97–103%. The immunosensor also displays high selectivity over other bacteria and acceptable stability. Presumably, our study can be regarded as the first one to report chicken IgY in order to detect
S. aureus
based on an electrochemical method.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-020-04547-6 |
| format | Article |
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Staphylococcus aureus
(
S. aureus
)
.
Chicken IgY as a capture antibody provides highly selective and specific binding to the target bacteria and selectively captures the
S. aureus
in its three-dimensional space. Due to that it can eliminate the interference from protein G-producing Streptococcus. In addition, the electron-transfer characteristic of [Fe(CN)
6
]
4−/3−
is hindered by this combination; as it is reflected on the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) curves. The proposed immunosensor displays a wide linear dynamic range from 10 to 10
7
CFU mL
−1
with a detection limit of 3.3 CFU mL
−1
with RSD 3.0%. It is capable to accurately determine
S. aureus
in milk and human blood serum as a complex matrix sample with satisfactory recovery of ∼ 97–103%. The immunosensor also displays high selectivity over other bacteria and acceptable stability. Presumably, our study can be regarded as the first one to report chicken IgY in order to detect
S. aureus
based on an electrochemical method.
Graphical abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-020-04547-6</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Antibodies ; Bacteria ; Biological products ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Displays ; Electric properties ; Electrochemical impedance spectroscopy ; Electrodes ; Electron transport ; Glassy carbon ; Gold ; Immunosensors ; Iron cyanides ; Microengineering ; Milk ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Original Paper ; Penicillin ; Protein A ; Proteins ; Selectivity ; Staphylococcus aureus ; Staphylococcus aureus infections ; Viral antibodies</subject><ispartof>Mikrochimica acta (1966), 2020-10, Vol.187 (10), p.567-567, Article 567</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-91b7bbcca767b794c2051f9237720da58c9162e91ef9dbbbcba50c12d64153d63</citedby><cites>FETCH-LOGICAL-c419t-91b7bbcca767b794c2051f9237720da58c9162e91ef9dbbbcba50c12d64153d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-020-04547-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-020-04547-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Roushani, Mahmoud</creatorcontrib><creatorcontrib>Rahmati, Zeinab</creatorcontrib><creatorcontrib>Golchin, Mehdi</creatorcontrib><creatorcontrib>Lotfi, Zinat</creatorcontrib><creatorcontrib>Nemati, Mostafa</creatorcontrib><title>Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>A new ultrasensitive immunosensor is proposed based on the covalently attached anti-protein A antibody (IgY) on deposited gold nanoparticle (AuNP)-modified glassy carbon electrode (GCE) for the electrochemical measurement of
Staphylococcus aureus
(
S. aureus
)
.
Chicken IgY as a capture antibody provides highly selective and specific binding to the target bacteria and selectively captures the
S. aureus
in its three-dimensional space. Due to that it can eliminate the interference from protein G-producing Streptococcus. In addition, the electron-transfer characteristic of [Fe(CN)
6
]
4−/3−
is hindered by this combination; as it is reflected on the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) curves. The proposed immunosensor displays a wide linear dynamic range from 10 to 10
7
CFU mL
−1
with a detection limit of 3.3 CFU mL
−1
with RSD 3.0%. It is capable to accurately determine
S. aureus
in milk and human blood serum as a complex matrix sample with satisfactory recovery of ∼ 97–103%. The immunosensor also displays high selectivity over other bacteria and acceptable stability. Presumably, our study can be regarded as the first one to report chicken IgY in order to detect
S. aureus
based on an electrochemical method.
Graphical abstract</description><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Bacteria</subject><subject>Biological products</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Displays</subject><subject>Electric properties</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Electrodes</subject><subject>Electron transport</subject><subject>Glassy carbon</subject><subject>Gold</subject><subject>Immunosensors</subject><subject>Iron cyanides</subject><subject>Microengineering</subject><subject>Milk</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Penicillin</subject><subject>Protein A</subject><subject>Proteins</subject><subject>Selectivity</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus infections</subject><subject>Viral antibodies</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9ks2KFDEUhQtRsB19AVcBN25qTFL56VoOw6gDAy7UhauQpG5VZ0glbZJCel7I1zQ1JTSKSAiXG75zckNO07wm-JJgLN9ljAVmLaa4xYwz2YonzY6wTrQcy-5ps8OYirYTkj5vXuR8jzGRgrJd8_PGgy0p2gPMzmqP3DwvIWYIOSY01j1AgTS7oIuLAcURfS76eDj5aKO1S0Z6SVCL0bZyTiNzQrfTt9UnGufdAwyo6iavcz4hq5OpHWyXDoB-uHI4t8eYXamCKfoBBR3iUafirIf8snk2ap_h1e960Xx9f_Pl-mN79-nD7fXVXWsZ6UvbEyONsVZLIY3smaWYk7GnnZQUD5rvbU8EhZ7A2A-mkkZzbAkdBCO8G0R30bzdfI8pfl8gFzW7bMF7HSAuWVHG6J5xzEhF3_yF3sclhTrdIyUI2VN2pibtQbkwxpK0XU3VlSQcc8HZvlKX_6DqGtZviQFGV8__ENBNYFPMOcGojsnNOp0UwWqNhNoioWok1GMk1Pq4bhPlCocJ0nni_6h-ASg_vHw</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Roushani, Mahmoud</creator><creator>Rahmati, Zeinab</creator><creator>Golchin, Mehdi</creator><creator>Lotfi, Zinat</creator><creator>Nemati, Mostafa</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20201001</creationdate><title>Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles</title><author>Roushani, Mahmoud ; Rahmati, Zeinab ; Golchin, Mehdi ; Lotfi, Zinat ; Nemati, Mostafa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-91b7bbcca767b794c2051f9237720da58c9162e91ef9dbbbcba50c12d64153d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Bacteria</topic><topic>Biological products</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Displays</topic><topic>Electric properties</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Electrodes</topic><topic>Electron transport</topic><topic>Glassy carbon</topic><topic>Gold</topic><topic>Immunosensors</topic><topic>Iron cyanides</topic><topic>Microengineering</topic><topic>Milk</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Penicillin</topic><topic>Protein A</topic><topic>Proteins</topic><topic>Selectivity</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus infections</topic><topic>Viral antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roushani, Mahmoud</creatorcontrib><creatorcontrib>Rahmati, Zeinab</creatorcontrib><creatorcontrib>Golchin, Mehdi</creatorcontrib><creatorcontrib>Lotfi, Zinat</creatorcontrib><creatorcontrib>Nemati, Mostafa</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roushani, Mahmoud</au><au>Rahmati, Zeinab</au><au>Golchin, Mehdi</au><au>Lotfi, Zinat</au><au>Nemati, Mostafa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2020-10-01</date><risdate>2020</risdate><volume>187</volume><issue>10</issue><spage>567</spage><epage>567</epage><pages>567-567</pages><artnum>567</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A new ultrasensitive immunosensor is proposed based on the covalently attached anti-protein A antibody (IgY) on deposited gold nanoparticle (AuNP)-modified glassy carbon electrode (GCE) for the electrochemical measurement of
Staphylococcus aureus
(
S. aureus
)
.
Chicken IgY as a capture antibody provides highly selective and specific binding to the target bacteria and selectively captures the
S. aureus
in its three-dimensional space. Due to that it can eliminate the interference from protein G-producing Streptococcus. In addition, the electron-transfer characteristic of [Fe(CN)
6
]
4−/3−
is hindered by this combination; as it is reflected on the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) curves. The proposed immunosensor displays a wide linear dynamic range from 10 to 10
7
CFU mL
−1
with a detection limit of 3.3 CFU mL
−1
with RSD 3.0%. It is capable to accurately determine
S. aureus
in milk and human blood serum as a complex matrix sample with satisfactory recovery of ∼ 97–103%. The immunosensor also displays high selectivity over other bacteria and acceptable stability. Presumably, our study can be regarded as the first one to report chicken IgY in order to detect
S. aureus
based on an electrochemical method.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-020-04547-6</doi><tpages>1</tpages></addata></record> |
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| subjects | Analytical Chemistry Antibodies Bacteria Biological products Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Displays Electric properties Electrochemical impedance spectroscopy Electrodes Electron transport Glassy carbon Gold Immunosensors Iron cyanides Microengineering Milk Nanochemistry Nanoparticles Nanotechnology Original Paper Penicillin Protein A Proteins Selectivity Staphylococcus aureus Staphylococcus aureus infections Viral antibodies |
| title | Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles |
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