Prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in suspected diarrhoeic buffaloes and cattle reporting at Veterinary University in India

•Manuscript discusses the incidence of MAP infection in diarrhoeic bovines, a question which has never been evaluated.•Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.•Buffaloes positive for MAP infection had reduced total leukocyte count and lympho...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2020-12, Vol.73, p.101533-101533, Article 101533
Hauptverfasser: Sharma, Shalini, Gautam, Ajay, Singh, Shoor Vir, Chaubey, Kundan Kumar, Mehta, Rajat, Gupta, Saurabh, Sharma, Maneesh, Rose, Manoj Kumar, Jain, V.K.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 101533
container_issue
container_start_page 101533
container_title Comparative immunology, microbiology and infectious diseases
container_volume 73
creator Sharma, Shalini
Gautam, Ajay
Singh, Shoor Vir
Chaubey, Kundan Kumar
Mehta, Rajat
Gupta, Saurabh
Sharma, Maneesh
Rose, Manoj Kumar
Jain, V.K.
description •Manuscript discusses the incidence of MAP infection in diarrhoeic bovines, a question which has never been evaluated.•Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.•Buffaloes positive for MAP infection had reduced total leukocyte count and lymphocytes indicating chronic nature of disease.•Buffalo PBMCs express TIM3.•Elucidating relative expression of TIM3 on JD positive and negative PBMCs will provide a clue on immune exhaustion in JD. Murrah buffaloes, best breed for milk production are native of Haryana state. They contributes significantly to the farmer’s income, livelihood and food (milk and meat) security, in the semi-tropical regions of North India. Johne’s disease though endemic in the domestic livestock of the country, but reports are not available in the buffaloes suffering from morbidity due to progressive weakness and diarrhoea. We estimated the status of JD in diarrhoeic buffaloes and cattle reporting at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, using conventional, serological and PCR assays.141 buffaloes suffering from chronic diarrhoea were screened to estimate sero-prevalence of MAP and 50.0 % young and 53.52 % adult animals were positive. Of 14 cattle screened, none of the young and 66.6 % adult cows were positive. In buffaloes, 66.1 and 6.77 %, fecal samples were positive in microscopy and IS900 PCR, respectively. Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.Buffaloes positive for Mycobacterium avium subspecies paratuberculosis (MAP) infection had reduced total leukocyte count and lymphocytes.
doi_str_mv 10.1016/j.cimid.2020.101533
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2442843744</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0147957120301223</els_id><sourcerecordid>2442843744</sourcerecordid><originalsourceid>FETCH-LOGICAL-c336t-6f6a9422b0538f611d010ff638437678e9fba72414295d928bc75e198efe52b13</originalsourceid><addsrcrecordid>eNp9kc1O3DAUha0KpA60T9CNl3SRqf_iJAsWCBWKBCoLYGs5zjV4lLGD7Yw0L8Rz4jCs2diWdc537XMQ-kXJmhIq_2zWxm3dsGaEfdzUnH9DK9o2bUWlbI7QilDRVF3d0O_oJKUNIaSjgq7Q232EnR7BG8DB4ru9Cb02GaKbt1jvljXNfZrAOEh40lHnuYdo5jEkl_DZ3cX9b-y8BZNd8OVU5Is6w4AHp2N8CeAM7mdr9RgKQvsBG53zCDjCFGJ2_hnrjJ9gGep13ONH73YQk8v7hXfjC-cHOi7-BD8_91P0ePX34fJfdfv_-uby4rYynMtcSSt1JxjrSc1bKykdCCXWSt4K3simhc72umGCCtbVQ8fa3jQ10K4FCzXrKT9FZwfuFMPrDCmrrUsGxlF7CHNSTAi2sIQoUn6QmhhSimDVFN22vF9RopZW1EZ9tKKWVtShleI6P7ig_GLnIKpUki3pDy6W1NQQ3Jf-d9aTmgM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2442843744</pqid></control><display><type>article</type><title>Prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in suspected diarrhoeic buffaloes and cattle reporting at Veterinary University in India</title><source>Access via ScienceDirect (Elsevier)</source><creator>Sharma, Shalini ; Gautam, Ajay ; Singh, Shoor Vir ; Chaubey, Kundan Kumar ; Mehta, Rajat ; Gupta, Saurabh ; Sharma, Maneesh ; Rose, Manoj Kumar ; Jain, V.K.</creator><creatorcontrib>Sharma, Shalini ; Gautam, Ajay ; Singh, Shoor Vir ; Chaubey, Kundan Kumar ; Mehta, Rajat ; Gupta, Saurabh ; Sharma, Maneesh ; Rose, Manoj Kumar ; Jain, V.K.</creatorcontrib><description>•Manuscript discusses the incidence of MAP infection in diarrhoeic bovines, a question which has never been evaluated.•Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.•Buffaloes positive for MAP infection had reduced total leukocyte count and lymphocytes indicating chronic nature of disease.•Buffalo PBMCs express TIM3.•Elucidating relative expression of TIM3 on JD positive and negative PBMCs will provide a clue on immune exhaustion in JD. Murrah buffaloes, best breed for milk production are native of Haryana state. They contributes significantly to the farmer’s income, livelihood and food (milk and meat) security, in the semi-tropical regions of North India. Johne’s disease though endemic in the domestic livestock of the country, but reports are not available in the buffaloes suffering from morbidity due to progressive weakness and diarrhoea. We estimated the status of JD in diarrhoeic buffaloes and cattle reporting at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, using conventional, serological and PCR assays.141 buffaloes suffering from chronic diarrhoea were screened to estimate sero-prevalence of MAP and 50.0 % young and 53.52 % adult animals were positive. Of 14 cattle screened, none of the young and 66.6 % adult cows were positive. In buffaloes, 66.1 and 6.77 %, fecal samples were positive in microscopy and IS900 PCR, respectively. Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.Buffaloes positive for Mycobacterium avium subspecies paratuberculosis (MAP) infection had reduced total leukocyte count and lymphocytes.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2020.101533</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Diarrhoeic bovine ; Johne’s disease (JD) ; Mycobacterium avium subspecies paratuberculosis ; Paratuberculosis ; Sero-prevalence ; TIM3 ; TLC</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2020-12, Vol.73, p.101533-101533, Article 101533</ispartof><rights>2020 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c336t-6f6a9422b0538f611d010ff638437678e9fba72414295d928bc75e198efe52b13</citedby><cites>FETCH-LOGICAL-c336t-6f6a9422b0538f611d010ff638437678e9fba72414295d928bc75e198efe52b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cimid.2020.101533$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Sharma, Shalini</creatorcontrib><creatorcontrib>Gautam, Ajay</creatorcontrib><creatorcontrib>Singh, Shoor Vir</creatorcontrib><creatorcontrib>Chaubey, Kundan Kumar</creatorcontrib><creatorcontrib>Mehta, Rajat</creatorcontrib><creatorcontrib>Gupta, Saurabh</creatorcontrib><creatorcontrib>Sharma, Maneesh</creatorcontrib><creatorcontrib>Rose, Manoj Kumar</creatorcontrib><creatorcontrib>Jain, V.K.</creatorcontrib><title>Prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in suspected diarrhoeic buffaloes and cattle reporting at Veterinary University in India</title><title>Comparative immunology, microbiology and infectious diseases</title><description>•Manuscript discusses the incidence of MAP infection in diarrhoeic bovines, a question which has never been evaluated.•Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.•Buffaloes positive for MAP infection had reduced total leukocyte count and lymphocytes indicating chronic nature of disease.•Buffalo PBMCs express TIM3.•Elucidating relative expression of TIM3 on JD positive and negative PBMCs will provide a clue on immune exhaustion in JD. Murrah buffaloes, best breed for milk production are native of Haryana state. They contributes significantly to the farmer’s income, livelihood and food (milk and meat) security, in the semi-tropical regions of North India. Johne’s disease though endemic in the domestic livestock of the country, but reports are not available in the buffaloes suffering from morbidity due to progressive weakness and diarrhoea. We estimated the status of JD in diarrhoeic buffaloes and cattle reporting at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, using conventional, serological and PCR assays.141 buffaloes suffering from chronic diarrhoea were screened to estimate sero-prevalence of MAP and 50.0 % young and 53.52 % adult animals were positive. Of 14 cattle screened, none of the young and 66.6 % adult cows were positive. In buffaloes, 66.1 and 6.77 %, fecal samples were positive in microscopy and IS900 PCR, respectively. Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.Buffaloes positive for Mycobacterium avium subspecies paratuberculosis (MAP) infection had reduced total leukocyte count and lymphocytes.</description><subject>Diarrhoeic bovine</subject><subject>Johne’s disease (JD)</subject><subject>Mycobacterium avium subspecies paratuberculosis</subject><subject>Paratuberculosis</subject><subject>Sero-prevalence</subject><subject>TIM3</subject><subject>TLC</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc1O3DAUha0KpA60T9CNl3SRqf_iJAsWCBWKBCoLYGs5zjV4lLGD7Yw0L8Rz4jCs2diWdc537XMQ-kXJmhIq_2zWxm3dsGaEfdzUnH9DK9o2bUWlbI7QilDRVF3d0O_oJKUNIaSjgq7Q232EnR7BG8DB4ru9Cb02GaKbt1jvljXNfZrAOEh40lHnuYdo5jEkl_DZ3cX9b-y8BZNd8OVU5Is6w4AHp2N8CeAM7mdr9RgKQvsBG53zCDjCFGJ2_hnrjJ9gGep13ONH73YQk8v7hXfjC-cHOi7-BD8_91P0ePX34fJfdfv_-uby4rYynMtcSSt1JxjrSc1bKykdCCXWSt4K3simhc72umGCCtbVQ8fa3jQ10K4FCzXrKT9FZwfuFMPrDCmrrUsGxlF7CHNSTAi2sIQoUn6QmhhSimDVFN22vF9RopZW1EZ9tKKWVtShleI6P7ig_GLnIKpUki3pDy6W1NQQ3Jf-d9aTmgM</recordid><startdate>202012</startdate><enddate>202012</enddate><creator>Sharma, Shalini</creator><creator>Gautam, Ajay</creator><creator>Singh, Shoor Vir</creator><creator>Chaubey, Kundan Kumar</creator><creator>Mehta, Rajat</creator><creator>Gupta, Saurabh</creator><creator>Sharma, Maneesh</creator><creator>Rose, Manoj Kumar</creator><creator>Jain, V.K.</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202012</creationdate><title>Prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in suspected diarrhoeic buffaloes and cattle reporting at Veterinary University in India</title><author>Sharma, Shalini ; Gautam, Ajay ; Singh, Shoor Vir ; Chaubey, Kundan Kumar ; Mehta, Rajat ; Gupta, Saurabh ; Sharma, Maneesh ; Rose, Manoj Kumar ; Jain, V.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-6f6a9422b0538f611d010ff638437678e9fba72414295d928bc75e198efe52b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Diarrhoeic bovine</topic><topic>Johne’s disease (JD)</topic><topic>Mycobacterium avium subspecies paratuberculosis</topic><topic>Paratuberculosis</topic><topic>Sero-prevalence</topic><topic>TIM3</topic><topic>TLC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Shalini</creatorcontrib><creatorcontrib>Gautam, Ajay</creatorcontrib><creatorcontrib>Singh, Shoor Vir</creatorcontrib><creatorcontrib>Chaubey, Kundan Kumar</creatorcontrib><creatorcontrib>Mehta, Rajat</creatorcontrib><creatorcontrib>Gupta, Saurabh</creatorcontrib><creatorcontrib>Sharma, Maneesh</creatorcontrib><creatorcontrib>Rose, Manoj Kumar</creatorcontrib><creatorcontrib>Jain, V.K.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Shalini</au><au>Gautam, Ajay</au><au>Singh, Shoor Vir</au><au>Chaubey, Kundan Kumar</au><au>Mehta, Rajat</au><au>Gupta, Saurabh</au><au>Sharma, Maneesh</au><au>Rose, Manoj Kumar</au><au>Jain, V.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in suspected diarrhoeic buffaloes and cattle reporting at Veterinary University in India</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><date>2020-12</date><risdate>2020</risdate><volume>73</volume><spage>101533</spage><epage>101533</epage><pages>101533-101533</pages><artnum>101533</artnum><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>•Manuscript discusses the incidence of MAP infection in diarrhoeic bovines, a question which has never been evaluated.•Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.•Buffaloes positive for MAP infection had reduced total leukocyte count and lymphocytes indicating chronic nature of disease.•Buffalo PBMCs express TIM3.•Elucidating relative expression of TIM3 on JD positive and negative PBMCs will provide a clue on immune exhaustion in JD. Murrah buffaloes, best breed for milk production are native of Haryana state. They contributes significantly to the farmer’s income, livelihood and food (milk and meat) security, in the semi-tropical regions of North India. Johne’s disease though endemic in the domestic livestock of the country, but reports are not available in the buffaloes suffering from morbidity due to progressive weakness and diarrhoea. We estimated the status of JD in diarrhoeic buffaloes and cattle reporting at Veterinary Clinical Complex of Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India, using conventional, serological and PCR assays.141 buffaloes suffering from chronic diarrhoea were screened to estimate sero-prevalence of MAP and 50.0 % young and 53.52 % adult animals were positive. Of 14 cattle screened, none of the young and 66.6 % adult cows were positive. In buffaloes, 66.1 and 6.77 %, fecal samples were positive in microscopy and IS900 PCR, respectively. Sero-prevalence of JD was very high in diarrhoeic buffaloes and cattle from Haryana state of India.Buffaloes positive for Mycobacterium avium subspecies paratuberculosis (MAP) infection had reduced total leukocyte count and lymphocytes.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.cimid.2020.101533</doi><tpages>1</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0147-9571
ispartof Comparative immunology, microbiology and infectious diseases, 2020-12, Vol.73, p.101533-101533, Article 101533
issn 0147-9571
1878-1667
language eng
recordid cdi_proquest_miscellaneous_2442843744
source Access via ScienceDirect (Elsevier)
subjects Diarrhoeic bovine
Johne’s disease (JD)
Mycobacterium avium subspecies paratuberculosis
Paratuberculosis
Sero-prevalence
TIM3
TLC
title Prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) infection in suspected diarrhoeic buffaloes and cattle reporting at Veterinary University in India
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T16%3A40%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20of%20Mycobacterium%20avium%20subspecies%20paratuberculosis%20(MAP)%20infection%20in%20suspected%20diarrhoeic%20buffaloes%20and%20cattle%20reporting%20at%20Veterinary%20University%20in%20India&rft.jtitle=Comparative%20immunology,%20microbiology%20and%20infectious%20diseases&rft.au=Sharma,%20Shalini&rft.date=2020-12&rft.volume=73&rft.spage=101533&rft.epage=101533&rft.pages=101533-101533&rft.artnum=101533&rft.issn=0147-9571&rft.eissn=1878-1667&rft_id=info:doi/10.1016/j.cimid.2020.101533&rft_dat=%3Cproquest_cross%3E2442843744%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2442843744&rft_id=info:pmid/&rft_els_id=S0147957120301223&rfr_iscdi=true