A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea

A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation exper...

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Veröffentlicht in:	Journal of microbiological methods 2020-12, Vol.179, p.106053-106053, Article 106053
Hauptverfasser:	Nguyen, Dong Xuan, Nakazawa, Takehito, Myo, Genki, Inoue, Chikako, Kawauchi, Moriyuki, Sakamoto, Masahiro, Honda, Yoichi
Format:	Artikel
Sprache:	eng
Schlagworte:	CRISPR/Cas9 Gene targeting Mushroom Promoter assay Wood-rot fungi
Online-Zugang:	Volltext
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