A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea
A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation exper...
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| Veröffentlicht in: | Journal of microbiological methods 2020-12, Vol.179, p.106053-106053, Article 106053 |
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| container_end_page | 106053 |
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| container_start_page | 106053 |
| container_title | Journal of microbiological methods |
| container_volume | 179 |
| creator | Nguyen, Dong Xuan Nakazawa, Takehito Myo, Genki Inoue, Chikako Kawauchi, Moriyuki Sakamoto, Masahiro Honda, Yoichi |
| description | A novel promoter assay was developed for Agaricomycetes, using a gene-targeting approach, with or without the CRISPR/Cas9 technique. It enables precise evaluation of promoter activity at the original site of the chromosome without random and multiple integrations in conventional transformation experiments. |
| doi_str_mv | 10.1016/j.mimet.2020.106053 |
| format | Article |
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| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2020-12, Vol.179, p.106053-106053, Article 106053 |
| issn | 0167-7012 1872-8359 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2442220997 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | CRISPR/Cas9 Gene targeting Mushroom Promoter assay Wood-rot fungi |
| title | A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea |
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