Nanoelectrochemical quantification of single-cell metabolism
At the most fundamental level, the behavior of tissue is governed by the activity of its single cells. A detailed examination of single-cell biology is necessary in order to gain a deeper understanding of disease progression. While single-cell genomics and transcriptomics are mature due to robust am...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2021-01, Vol.413 (1), p.17-24 |
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| creator | McCormick, Hadley K. Dick, Jeffrey E. |
| description | At the most fundamental level, the behavior of tissue is governed by the activity of its single cells. A detailed examination of single-cell biology is necessary in order to gain a deeper understanding of disease progression. While single-cell genomics and transcriptomics are mature due to robust amplification strategies, the metabolome is difficult to quantify. Nanoelectrochemical techniques stand poised to quantify single-cell metabolism as a result of the fabrication of nanoelectrodes, which allow one to make intracellular electrochemical measurements. This article is concerned with intracellular nanoelectrochemistry, focusing on the sensitive and selective quantification of various metabolites within a single, living cell. We will review the strong literature behind this field, discuss the potential deleterious effects of passing charge inside cells, and provide future outlooks for this promising avenue of inquiry. We also present a mathematical relationship based on Faraday’s Law and bulk electrolysis theory to examine the consumption of analyte within a cell due to passing charge at the nanotip.
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| doi_str_mv | 10.1007/s00216-020-02899-9 |
| format | Article |
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We also present a mathematical relationship based on Faraday’s Law and bulk electrolysis theory to examine the consumption of analyte within a cell due to passing charge at the nanotip.
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| subjects | Analysis Analytical Chemistry Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Electrochemical analysis Electrochemistry Electrolysis Electrolytic cells Fabrication Food Science Intracellular Laboratory Medicine Literature reviews Metabolism Metabolites Monitoring/Environmental Analysis Nanotechnology Physiological aspects Robustness (mathematics) Technology application Trends Unicellular organisms |
| title | Nanoelectrochemical quantification of single-cell metabolism |
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