Effects of microRNA-338 Transfection into Sciatic Nerve on Rats with Experimental Autoimmune Neuritis
Nerve demyelination or axonal lesions are characteristic of experimental autoimmune neuritis (EAN). Previous studies have demonstrated that microRNA-338 can regulate the differentiation and maturation of oligodendrocytes and Schwann cells and promote injured peripheral nerves in rats. In this study,...
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| Veröffentlicht in: | Journal of molecular neuroscience 2021-04, Vol.71 (4), p.713-723 |
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| creator | Yuan, Xiaojing Wei, Yujun Ao, Tianrang Gong, Kai Sun, Qiangsan Zheng, Zuncheng Hagiwara, Haruo Ao, Qiang |
| description | Nerve demyelination or axonal lesions are characteristic of experimental autoimmune neuritis (EAN). Previous studies have demonstrated that microRNA-338 can regulate the differentiation and maturation of oligodendrocytes and Schwann cells and promote injured peripheral nerves in rats. In this study, we used microRNA-338 coded lentivirus vector (miR-338-LV) in a Lewis rat EAN model, in with the conjunction P0 peptide 180–199 which was injected into the footpads of animals to induce immunization. The clinical scores of miR-338-LV and intravenous immunoglobulin (IVIg) (positive drug) groups were significantly superior to those of untreated group at disease peak and disease plateau (
p
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| doi_str_mv | 10.1007/s12031-020-01689-3 |
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p
< 0.05). The nerve conduction velocity and the compound nerve action potential amplitude of miR-338-LV and IVIg groups increased significantly compared to those of the untreated group at disease peak (
p
< 0.01). At disease peak, myelin swelling, cavity formation, and lamellae separation showed improvement in miR-338-LV and IVIg groups compared to untreated group. S100 and NF200 expression in miR-338-LV and IVIg groups increased compared to that in untreated group. Iba1 and S100 co-expression in Schwann cells in miR-338-LV and IVIg groups decreased compared to that in untreated group, which was indicative of the reduced conversion of Schwann cells into inflammatory cells. Overall, miR-338-LV in sciatic nerves might improve neuromuscular function in EAN by inhibiting the conversion of Schwann cells into inflammatory cells.</description><identifier>ISSN: 0895-8696</identifier><identifier>EISSN: 1559-1166</identifier><identifier>DOI: 10.1007/s12031-020-01689-3</identifier><identifier>PMID: 32915416</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Action potential ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Conversion ; Demyelination ; Experimental allergic neuritis ; Immunization ; Immunoglobulins ; Inflammation ; Intravenous administration ; Lamellae ; MicroRNAs ; miRNA ; Myelin ; Myelin P0 protein ; Nerve conduction ; Neuritis ; Neurochemistry ; Neurology ; Neurosciences ; Oligodendrocytes ; Peripheral nerves ; Proteomics ; Ribonucleic acid ; RNA ; Schwann cells ; Sciatic nerve ; Transfection</subject><ispartof>Journal of molecular neuroscience, 2021-04, Vol.71 (4), p.713-723</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-59b4d2a2e1528071c973b28c20ae7e607ae30c9fb747df7b2efc05cca754cf8e3</citedby><cites>FETCH-LOGICAL-c375t-59b4d2a2e1528071c973b28c20ae7e607ae30c9fb747df7b2efc05cca754cf8e3</cites><orcidid>0000-0002-1309-1971</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12031-020-01689-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12031-020-01689-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32915416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Xiaojing</creatorcontrib><creatorcontrib>Wei, Yujun</creatorcontrib><creatorcontrib>Ao, Tianrang</creatorcontrib><creatorcontrib>Gong, Kai</creatorcontrib><creatorcontrib>Sun, Qiangsan</creatorcontrib><creatorcontrib>Zheng, Zuncheng</creatorcontrib><creatorcontrib>Hagiwara, Haruo</creatorcontrib><creatorcontrib>Ao, Qiang</creatorcontrib><title>Effects of microRNA-338 Transfection into Sciatic Nerve on Rats with Experimental Autoimmune Neuritis</title><title>Journal of molecular neuroscience</title><addtitle>J Mol Neurosci</addtitle><addtitle>J Mol Neurosci</addtitle><description>Nerve demyelination or axonal lesions are characteristic of experimental autoimmune neuritis (EAN). Previous studies have demonstrated that microRNA-338 can regulate the differentiation and maturation of oligodendrocytes and Schwann cells and promote injured peripheral nerves in rats. In this study, we used microRNA-338 coded lentivirus vector (miR-338-LV) in a Lewis rat EAN model, in with the conjunction P0 peptide 180–199 which was injected into the footpads of animals to induce immunization. The clinical scores of miR-338-LV and intravenous immunoglobulin (IVIg) (positive drug) groups were significantly superior to those of untreated group at disease peak and disease plateau (
p
< 0.05). The nerve conduction velocity and the compound nerve action potential amplitude of miR-338-LV and IVIg groups increased significantly compared to those of the untreated group at disease peak (
p
< 0.01). At disease peak, myelin swelling, cavity formation, and lamellae separation showed improvement in miR-338-LV and IVIg groups compared to untreated group. S100 and NF200 expression in miR-338-LV and IVIg groups increased compared to that in untreated group. Iba1 and S100 co-expression in Schwann cells in miR-338-LV and IVIg groups decreased compared to that in untreated group, which was indicative of the reduced conversion of Schwann cells into inflammatory cells. Overall, miR-338-LV in sciatic nerves might improve neuromuscular function in EAN by inhibiting the conversion of Schwann cells into inflammatory cells.</description><subject>Action potential</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Conversion</subject><subject>Demyelination</subject><subject>Experimental allergic neuritis</subject><subject>Immunization</subject><subject>Immunoglobulins</subject><subject>Inflammation</subject><subject>Intravenous administration</subject><subject>Lamellae</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Myelin</subject><subject>Myelin P0 protein</subject><subject>Nerve conduction</subject><subject>Neuritis</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Oligodendrocytes</subject><subject>Peripheral nerves</subject><subject>Proteomics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Schwann cells</subject><subject>Sciatic nerve</subject><subject>Transfection</subject><issn>0895-8696</issn><issn>1559-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kUFvFSEUhYmxsc_qH3BhSNy4wV5ggGH50jy1SdMmta4Jw7sozZuZJzBW_73UVzVx0RUJ9zsH7jmEvOLwjgOY08IFSM5AAAOue8vkE7LiSlnGudZPyQp6q1ivrT4mz0u5BRC84_0zciyF5arjekVwEyOGWugc6ZhCnq8v10zKnt5kP5X7UZonmqY6008h-ZoCvcT8HWm7vfZNd5fqV7r5scecRpyq39H1Uuc0jsuEDV1yqqm8IEfR7wq-fDhPyOf3m5uzj-zi6sP52fqCBWlUZcoO3VZ4gVyJHgwP1shB9EGAR4MajEcJwcbBdGYbzSAwBlAheKO6EHuUJ-TtwXef528LlurGVALudn7CeSlOdG1p0Eqbhr75D72dlzy13zmhWqSdNFY1ShyolkwpGaPbtz19_uk4uPsS3KEE1xTudwlONtHrB-tlGHH7V_In9QbIA1DaaPqC-d_bj9j-AueskZ8</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Yuan, Xiaojing</creator><creator>Wei, Yujun</creator><creator>Ao, Tianrang</creator><creator>Gong, Kai</creator><creator>Sun, Qiangsan</creator><creator>Zheng, Zuncheng</creator><creator>Hagiwara, Haruo</creator><creator>Ao, Qiang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1309-1971</orcidid></search><sort><creationdate>20210401</creationdate><title>Effects of microRNA-338 Transfection into Sciatic Nerve on Rats with Experimental Autoimmune Neuritis</title><author>Yuan, Xiaojing ; Wei, Yujun ; Ao, Tianrang ; Gong, Kai ; Sun, Qiangsan ; Zheng, Zuncheng ; Hagiwara, Haruo ; Ao, Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-59b4d2a2e1528071c973b28c20ae7e607ae30c9fb747df7b2efc05cca754cf8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Action potential</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Conversion</topic><topic>Demyelination</topic><topic>Experimental allergic neuritis</topic><topic>Immunization</topic><topic>Immunoglobulins</topic><topic>Inflammation</topic><topic>Intravenous administration</topic><topic>Lamellae</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Myelin</topic><topic>Myelin P0 protein</topic><topic>Nerve conduction</topic><topic>Neuritis</topic><topic>Neurochemistry</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Oligodendrocytes</topic><topic>Peripheral nerves</topic><topic>Proteomics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Schwann cells</topic><topic>Sciatic nerve</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Xiaojing</creatorcontrib><creatorcontrib>Wei, Yujun</creatorcontrib><creatorcontrib>Ao, Tianrang</creatorcontrib><creatorcontrib>Gong, Kai</creatorcontrib><creatorcontrib>Sun, Qiangsan</creatorcontrib><creatorcontrib>Zheng, Zuncheng</creatorcontrib><creatorcontrib>Hagiwara, Haruo</creatorcontrib><creatorcontrib>Ao, Qiang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Xiaojing</au><au>Wei, Yujun</au><au>Ao, Tianrang</au><au>Gong, Kai</au><au>Sun, Qiangsan</au><au>Zheng, Zuncheng</au><au>Hagiwara, Haruo</au><au>Ao, Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of microRNA-338 Transfection into Sciatic Nerve on Rats with Experimental Autoimmune Neuritis</atitle><jtitle>Journal of molecular neuroscience</jtitle><stitle>J Mol Neurosci</stitle><addtitle>J Mol Neurosci</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>71</volume><issue>4</issue><spage>713</spage><epage>723</epage><pages>713-723</pages><issn>0895-8696</issn><eissn>1559-1166</eissn><abstract>Nerve demyelination or axonal lesions are characteristic of experimental autoimmune neuritis (EAN). Previous studies have demonstrated that microRNA-338 can regulate the differentiation and maturation of oligodendrocytes and Schwann cells and promote injured peripheral nerves in rats. In this study, we used microRNA-338 coded lentivirus vector (miR-338-LV) in a Lewis rat EAN model, in with the conjunction P0 peptide 180–199 which was injected into the footpads of animals to induce immunization. The clinical scores of miR-338-LV and intravenous immunoglobulin (IVIg) (positive drug) groups were significantly superior to those of untreated group at disease peak and disease plateau (
p
< 0.05). The nerve conduction velocity and the compound nerve action potential amplitude of miR-338-LV and IVIg groups increased significantly compared to those of the untreated group at disease peak (
p
< 0.01). At disease peak, myelin swelling, cavity formation, and lamellae separation showed improvement in miR-338-LV and IVIg groups compared to untreated group. S100 and NF200 expression in miR-338-LV and IVIg groups increased compared to that in untreated group. Iba1 and S100 co-expression in Schwann cells in miR-338-LV and IVIg groups decreased compared to that in untreated group, which was indicative of the reduced conversion of Schwann cells into inflammatory cells. Overall, miR-338-LV in sciatic nerves might improve neuromuscular function in EAN by inhibiting the conversion of Schwann cells into inflammatory cells.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32915416</pmid><doi>10.1007/s12031-020-01689-3</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1309-1971</orcidid></addata></record> |
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| subjects | Action potential Biomedical and Life Sciences Biomedicine Cell Biology Conversion Demyelination Experimental allergic neuritis Immunization Immunoglobulins Inflammation Intravenous administration Lamellae MicroRNAs miRNA Myelin Myelin P0 protein Nerve conduction Neuritis Neurochemistry Neurology Neurosciences Oligodendrocytes Peripheral nerves Proteomics Ribonucleic acid RNA Schwann cells Sciatic nerve Transfection |
| title | Effects of microRNA-338 Transfection into Sciatic Nerve on Rats with Experimental Autoimmune Neuritis |
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