Inhibition of E. coli Host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness

With the growing interest in continuous cultivation of Escherichia coli, secretion of product to the medium is not only a benefit, but a necessity in future bioprocessing. In this study, it is shown that induced decoupling of growth and heterologous gene expression in the E. coli X‐press strain (der...

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Veröffentlicht in:	Biotechnology journal 2021-03, Vol.16 (3), p.e2000274-n/a
Hauptverfasser:	Kastenhofer, Jens, Rettenbacher, Lukas, Feuchtenhofer, Lukas, Mairhofer, Juergen, Spadiut, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	continuous manufacturing Culture Media DNA-Directed RNA Polymerases - genetics Escherichia coli - genetics Escherichia coli Proteins leakiness outer membrane integrity periplasmic protein release Recombinant Proteins - genetics secretion
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