A Lipid Transfer Protein has Antifungal and Antioxidant Activity and Suppresses Fusarium Head Blight Disease and DON Accumulation in Transgenic Wheat
Trichothecene mycotoxins such as deoxynivalenol (DON) are virulence factors of , which causes Fusarium head blight, one of the most important diseases of small grain cereals. We previously identified a nonspecific lipid transfer protein (nsLTP) gene, , which was overexpressed in an activation-tagged...
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| Veröffentlicht in: | Phytopathology 2021-04, Vol.111 (4), p.671-683 |
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| creator | McLaughlin, John E Darwish, Noura I Garcia-Sanchez, Jeffrey Tyagi, Neerja Trick, Harold N McCormick, Susan Dill-Macky, Ruth Tumer, Nilgun E |
| description | Trichothecene mycotoxins such as deoxynivalenol (DON) are virulence factors of
, which causes Fusarium head blight, one of the most important diseases of small grain cereals. We previously identified a nonspecific lipid transfer protein (nsLTP) gene,
, which was overexpressed in an activation-tagged
line resistant to trichothecin, a type B trichothecene in the same class as DON. Here we show that overexpression of
in transgenic wheat significantly reduced
growth in 'Bobwhite' and 'RB07' lines in the greenhouse and reduced fungal lesion size in detached leaf assays. Hydrogen peroxide accumulation was attenuated on exposure of transgenic wheat plants to DON, indicating that
may confer resistance by inhibiting oxidative stress. Field testing indicated that disease severity was significantly reduced in two transgenic 'Bobwhite' lines expressing
DON accumulation was significantly reduced in four different transgenic 'Bobwhite' lines expressing
or a wheat nsLTP,
, which was previously shown to have antioxidant activity. Recombinant
purified from
exhibited potent antifungal activity against
. These results demonstrate that overexpression of
in transgenic wheat suppresses DON accumulation in the field. Suppression of DON-induced reactive oxygen species by
might be the mechanism by which fungal spread and mycotoxin accumulation are inhibited in transgenic wheat plants. |
| doi_str_mv | 10.1094/PHYTO-04-20-0153-R |
| format | Article |
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, which causes Fusarium head blight, one of the most important diseases of small grain cereals. We previously identified a nonspecific lipid transfer protein (nsLTP) gene,
, which was overexpressed in an activation-tagged
line resistant to trichothecin, a type B trichothecene in the same class as DON. Here we show that overexpression of
in transgenic wheat significantly reduced
growth in 'Bobwhite' and 'RB07' lines in the greenhouse and reduced fungal lesion size in detached leaf assays. Hydrogen peroxide accumulation was attenuated on exposure of transgenic wheat plants to DON, indicating that
may confer resistance by inhibiting oxidative stress. Field testing indicated that disease severity was significantly reduced in two transgenic 'Bobwhite' lines expressing
DON accumulation was significantly reduced in four different transgenic 'Bobwhite' lines expressing
or a wheat nsLTP,
, which was previously shown to have antioxidant activity. Recombinant
purified from
exhibited potent antifungal activity against
. These results demonstrate that overexpression of
in transgenic wheat suppresses DON accumulation in the field. Suppression of DON-induced reactive oxygen species by
might be the mechanism by which fungal spread and mycotoxin accumulation are inhibited in transgenic wheat plants.</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/PHYTO-04-20-0153-R</identifier><identifier>PMID: 32896217</identifier><language>eng</language><publisher>United States</publisher><subject>Antifungal Agents - pharmacology ; Antioxidants ; Carrier Proteins ; Fusarium ; Plant Diseases ; Saccharomycetales ; Triticum - genetics</subject><ispartof>Phytopathology, 2021-04, Vol.111 (4), p.671-683</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-41dac18e975ba3290ffdd453c6582bdb62624d67493fd294eea29479f1714bde3</citedby><cites>FETCH-LOGICAL-c347t-41dac18e975ba3290ffdd453c6582bdb62624d67493fd294eea29479f1714bde3</cites><orcidid>0000-0002-1693-2940</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3710,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32896217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McLaughlin, John E</creatorcontrib><creatorcontrib>Darwish, Noura I</creatorcontrib><creatorcontrib>Garcia-Sanchez, Jeffrey</creatorcontrib><creatorcontrib>Tyagi, Neerja</creatorcontrib><creatorcontrib>Trick, Harold N</creatorcontrib><creatorcontrib>McCormick, Susan</creatorcontrib><creatorcontrib>Dill-Macky, Ruth</creatorcontrib><creatorcontrib>Tumer, Nilgun E</creatorcontrib><title>A Lipid Transfer Protein has Antifungal and Antioxidant Activity and Suppresses Fusarium Head Blight Disease and DON Accumulation in Transgenic Wheat</title><title>Phytopathology</title><addtitle>Phytopathology</addtitle><description>Trichothecene mycotoxins such as deoxynivalenol (DON) are virulence factors of
, which causes Fusarium head blight, one of the most important diseases of small grain cereals. We previously identified a nonspecific lipid transfer protein (nsLTP) gene,
, which was overexpressed in an activation-tagged
line resistant to trichothecin, a type B trichothecene in the same class as DON. Here we show that overexpression of
in transgenic wheat significantly reduced
growth in 'Bobwhite' and 'RB07' lines in the greenhouse and reduced fungal lesion size in detached leaf assays. Hydrogen peroxide accumulation was attenuated on exposure of transgenic wheat plants to DON, indicating that
may confer resistance by inhibiting oxidative stress. Field testing indicated that disease severity was significantly reduced in two transgenic 'Bobwhite' lines expressing
DON accumulation was significantly reduced in four different transgenic 'Bobwhite' lines expressing
or a wheat nsLTP,
, which was previously shown to have antioxidant activity. Recombinant
purified from
exhibited potent antifungal activity against
. These results demonstrate that overexpression of
in transgenic wheat suppresses DON accumulation in the field. Suppression of DON-induced reactive oxygen species by
might be the mechanism by which fungal spread and mycotoxin accumulation are inhibited in transgenic wheat plants.</description><subject>Antifungal Agents - pharmacology</subject><subject>Antioxidants</subject><subject>Carrier Proteins</subject><subject>Fusarium</subject><subject>Plant Diseases</subject><subject>Saccharomycetales</subject><subject>Triticum - genetics</subject><issn>0031-949X</issn><issn>1943-7684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9Uctu1DAUtRAVHQo_wAJ5ycbgVx5eDi3tII06VRkErCwnvpkxSpzgR0U_hP8lTQube3V1z0M6B6E3jL5nVMkPN5sf-x2hknBKKCsEuX2GVkxJQaqyls_RilLBiJLq-yl6GeNPSmlVF-ULdCp4rUrOqhX6s8ZbNzmL98H42EHAN2FM4Dw-mojXPrku-4PpsfF2Ocffzhqf8LpN7s6l--XxJU9TgBgh4sscTXB5wBswFn_s3eGY8IWLYCIs2Ivd9Uxu85B7M8t5PHst5gfwrsXfjmDSK3TSmT7C66d9hr5eftqfb8h2d_X5fL0lrZBVIpJZ07IaVFU0RnBFu85aWYi2LGre2KbkJZe2rKQSneVKAph5VqpjFZONBXGG3j3qTmH8lSEmPbjYQt8bD2OOmktJazVHJWcof4S2YYwxQKen4AYT7jWj-qEOvdShqdSc6oc69O1Mevukn5sB7H_Kv_zFX4VMiHc</recordid><startdate>202104</startdate><enddate>202104</enddate><creator>McLaughlin, John E</creator><creator>Darwish, Noura I</creator><creator>Garcia-Sanchez, Jeffrey</creator><creator>Tyagi, Neerja</creator><creator>Trick, Harold N</creator><creator>McCormick, Susan</creator><creator>Dill-Macky, Ruth</creator><creator>Tumer, Nilgun E</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1693-2940</orcidid></search><sort><creationdate>202104</creationdate><title>A Lipid Transfer Protein has Antifungal and Antioxidant Activity and Suppresses Fusarium Head Blight Disease and DON Accumulation in Transgenic Wheat</title><author>McLaughlin, John E ; Darwish, Noura I ; Garcia-Sanchez, Jeffrey ; Tyagi, Neerja ; Trick, Harold N ; McCormick, Susan ; Dill-Macky, Ruth ; Tumer, Nilgun E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-41dac18e975ba3290ffdd453c6582bdb62624d67493fd294eea29479f1714bde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antifungal Agents - pharmacology</topic><topic>Antioxidants</topic><topic>Carrier Proteins</topic><topic>Fusarium</topic><topic>Plant Diseases</topic><topic>Saccharomycetales</topic><topic>Triticum - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McLaughlin, John E</creatorcontrib><creatorcontrib>Darwish, Noura I</creatorcontrib><creatorcontrib>Garcia-Sanchez, Jeffrey</creatorcontrib><creatorcontrib>Tyagi, Neerja</creatorcontrib><creatorcontrib>Trick, Harold N</creatorcontrib><creatorcontrib>McCormick, Susan</creatorcontrib><creatorcontrib>Dill-Macky, Ruth</creatorcontrib><creatorcontrib>Tumer, Nilgun E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McLaughlin, John E</au><au>Darwish, Noura I</au><au>Garcia-Sanchez, Jeffrey</au><au>Tyagi, Neerja</au><au>Trick, Harold N</au><au>McCormick, Susan</au><au>Dill-Macky, Ruth</au><au>Tumer, Nilgun E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Lipid Transfer Protein has Antifungal and Antioxidant Activity and Suppresses Fusarium Head Blight Disease and DON Accumulation in Transgenic Wheat</atitle><jtitle>Phytopathology</jtitle><addtitle>Phytopathology</addtitle><date>2021-04</date><risdate>2021</risdate><volume>111</volume><issue>4</issue><spage>671</spage><epage>683</epage><pages>671-683</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><abstract>Trichothecene mycotoxins such as deoxynivalenol (DON) are virulence factors of
, which causes Fusarium head blight, one of the most important diseases of small grain cereals. We previously identified a nonspecific lipid transfer protein (nsLTP) gene,
, which was overexpressed in an activation-tagged
line resistant to trichothecin, a type B trichothecene in the same class as DON. Here we show that overexpression of
in transgenic wheat significantly reduced
growth in 'Bobwhite' and 'RB07' lines in the greenhouse and reduced fungal lesion size in detached leaf assays. Hydrogen peroxide accumulation was attenuated on exposure of transgenic wheat plants to DON, indicating that
may confer resistance by inhibiting oxidative stress. Field testing indicated that disease severity was significantly reduced in two transgenic 'Bobwhite' lines expressing
DON accumulation was significantly reduced in four different transgenic 'Bobwhite' lines expressing
or a wheat nsLTP,
, which was previously shown to have antioxidant activity. Recombinant
purified from
exhibited potent antifungal activity against
. These results demonstrate that overexpression of
in transgenic wheat suppresses DON accumulation in the field. Suppression of DON-induced reactive oxygen species by
might be the mechanism by which fungal spread and mycotoxin accumulation are inhibited in transgenic wheat plants.</abstract><cop>United States</cop><pmid>32896217</pmid><doi>10.1094/PHYTO-04-20-0153-R</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-1693-2940</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | Antifungal Agents - pharmacology Antioxidants Carrier Proteins Fusarium Plant Diseases Saccharomycetales Triticum - genetics |
| title | A Lipid Transfer Protein has Antifungal and Antioxidant Activity and Suppresses Fusarium Head Blight Disease and DON Accumulation in Transgenic Wheat |
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