Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus)
The bacteriophage vB_AhM_PVN02 (PVN02), infecting Aeromonas hydrophila , was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively....
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| Veröffentlicht in: | Archives of virology 2020-12, Vol.165 (12), p.2973-2977 |
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| creator | Tu, Vinh Q. Nguyen, Tan-Trung Tran, Xuan T. T. Millard, Andrew D. Phan, Huyen T. Le, Nga P. Dang, Oanh T. H. Hoang, Hoang A. |
| description | The bacteriophage vB_AhM_PVN02 (PVN02), infecting
Aeromonas hydrophila
, was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family
Myoviridae
. The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family
Myoviridae
. This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control
Aeromonas hydrophila
in striped catfish in the Mekong Delta, Vietnam. |
| doi_str_mv | 10.1007/s00705-020-04793-2 |
| format | Article |
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Aeromonas hydrophila
, was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family
Myoviridae
. The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family
Myoviridae
. This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control
Aeromonas hydrophila
in striped catfish in the Mekong Delta, Vietnam.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-020-04793-2</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Aeromonas hydrophila ; Annotated Sequence Record ; Antibiotic resistance ; Biological control ; Biomedical and Life Sciences ; Biomedicine ; Burst size ; Genomes ; Infectious Diseases ; Latent period ; Medical Microbiology ; Myoviridae ; New species ; Nucleotide sequence ; Phages ; Phylogeny ; Transmission electron microscopy ; Virology ; Virulence</subject><ispartof>Archives of virology, 2020-12, Vol.165 (12), p.2973-2977</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2672-c9df38866bab53baff5b7bb1156eb0d65e01e66d18efa3478bebe7c3672b8dae3</citedby><cites>FETCH-LOGICAL-c2672-c9df38866bab53baff5b7bb1156eb0d65e01e66d18efa3478bebe7c3672b8dae3</cites><orcidid>0000-0001-8874-0705</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-020-04793-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-020-04793-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids></links><search><creatorcontrib>Tu, Vinh Q.</creatorcontrib><creatorcontrib>Nguyen, Tan-Trung</creatorcontrib><creatorcontrib>Tran, Xuan T. T.</creatorcontrib><creatorcontrib>Millard, Andrew D.</creatorcontrib><creatorcontrib>Phan, Huyen T.</creatorcontrib><creatorcontrib>Le, Nga P.</creatorcontrib><creatorcontrib>Dang, Oanh T. H.</creatorcontrib><creatorcontrib>Hoang, Hoang A.</creatorcontrib><title>Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus)</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>The bacteriophage vB_AhM_PVN02 (PVN02), infecting
Aeromonas hydrophila
, was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family
Myoviridae
. The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family
Myoviridae
. This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control
Aeromonas hydrophila
in striped catfish in the Mekong Delta, Vietnam.</description><subject>Aeromonas hydrophila</subject><subject>Annotated Sequence Record</subject><subject>Antibiotic resistance</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Burst size</subject><subject>Genomes</subject><subject>Infectious Diseases</subject><subject>Latent period</subject><subject>Medical Microbiology</subject><subject>Myoviridae</subject><subject>New species</subject><subject>Nucleotide sequence</subject><subject>Phages</subject><subject>Phylogeny</subject><subject>Transmission electron microscopy</subject><subject>Virology</subject><subject>Virulence</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU2L1TAUhoMoeB39A64CbkawmjRNky6Hy_gBA7rQdUjSkzZDm9SkV7j_wx_s0asILtwkcHiel5O8hDzn7DVnTL2peDDZsJY1rFODaNoH5MA70TZaDfohOTDBukb3TD8mT2q9ZwwHQh7I92NetwV2oBOkvAKt8PUEyQPNgVqa8jdY6HLeo6fbbCegMQXwe0wTvYGS15xspfN5LHmb42JfUZv-IPlUKRppxwGte4kbjNTbPcQ60-tPNk22RpvymBMmbBiwz3ZZT_XlU_Io2KXCs9_3Ffny9vbz8X1z9_Hdh-PNXePbXrWNH8YgtO57Z50UzoYgnXKOc9mDY2MvgXHo-5FrCFZ0SjtwoLxA1-nRgrgi15fcrWR8dd3NGquHZbEJcHvTdh3-JusGieiLf9D7fCoJt0NKcamkFgNS7YXyJddaIJitxNWWs-HM_CzKXIoyWJT5VZRpURIXqSKcJih_o_9j_QDCMpl5</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Tu, Vinh Q.</creator><creator>Nguyen, Tan-Trung</creator><creator>Tran, Xuan T. 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T.</au><au>Millard, Andrew D.</au><au>Phan, Huyen T.</au><au>Le, Nga P.</au><au>Dang, Oanh T. H.</au><au>Hoang, Hoang A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus)</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><date>2020-12-01</date><risdate>2020</risdate><volume>165</volume><issue>12</issue><spage>2973</spage><epage>2977</epage><pages>2973-2977</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>The bacteriophage vB_AhM_PVN02 (PVN02), infecting
Aeromonas hydrophila
, was isolated from a striped catfish pond water sample in Can Tho City, Vietnam. The phage had high lytic activity with a latent period and burst size of approximately 20 min and 105 plaque-forming units per cell, respectively. Observation of the phage by transmission electron microscopy indicated that PVN02 belongs to the family
Myoviridae
. The genome of PVN02 is a double-stranded linear DNA with a length in 51,668 bp and a content of 52% GC. Among the 64 genes, 16 were predicted to encode proteins with predicted functions. No virulence or antibiotic resistance genes were found in the genome, suggesting it would be a useful biocontrol agent. Classification of the phage based on sequence comparisons, phylogenetic analysis, and gene-sharing networks was carried out, and it was found to be the first representative of a new species within a previously undefined genus in the family
Myoviridae
. This study confirmed that PVN02 is a novel lytic phage that could potentially be used as an agent to control
Aeromonas hydrophila
in striped catfish in the Mekong Delta, Vietnam.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00705-020-04793-2</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-8874-0705</orcidid></addata></record> |
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| identifier | ISSN: 0304-8608 |
| ispartof | Archives of virology, 2020-12, Vol.165 (12), p.2973-2977 |
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| language | eng |
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| source | SpringerNature Journals |
| subjects | Aeromonas hydrophila Annotated Sequence Record Antibiotic resistance Biological control Biomedical and Life Sciences Biomedicine Burst size Genomes Infectious Diseases Latent period Medical Microbiology Myoviridae New species Nucleotide sequence Phages Phylogeny Transmission electron microscopy Virology Virulence |
| title | Complete genome sequence of a novel lytic phage infecting Aeromonas hydrophila, an infectious agent in striped catfish (Pangasianodon hypophthalmus) |
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