Evaluation of the biological properties of two experimental calcium silicate sealers: an in vivo study in rats

Aim To evaluate the biological properties of experimental sealers based on tricalcium silicate and dicalcium silicate, manipulated with polyethylene glycol (CE‐1) and with the addition of calcium hypochlorite (CE‐2) compared to AH Plus (AHP) and TotalFill BC Sealer (TBC). Methodology The tissue reaction caused by the materials in the subcutaneous tissue of rats was evaluated after implantation of polyethylene tubes filled with the materials at 7, 15, 30 and 60 days. Sections were stained with haematoxylin and eosin (HE) for morphological analysis and to evaluated number of inflammatory cells/mm2 (ICs). Sections were used for immunohistochemical detection of interleukin‐6 (IL‐6) and osteocalcin (OC). The von Kossa method was used to identify calcium precipitation in the capsules. The data were submitted to anova and Tukey’s tests, with 5% significance level. Results At 7 days, CE‐1, CE‐2 and AHP had higher numbers of ICs. AHP presented higher immunolabelling for IL‐6. After 15 days, regarding IL‐6, there was no difference between CE‐2 and the control group. At 30 days, AHP exhibited the highest number of IC (P