A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system
Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to Salmonella typhimurium ( S. typhimurium ), as organic components, were embedded in organic-inorganic nanocompo...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2020-11, Vol.412 (28), p.7955-7962 |
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| container_issue | 28 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Zhang, Wenguang Bu, Shengjun Bai, Huasong Ma, Chengyou Ma, Li Wei, Hongguo Liu, Xiu Li, Zehong Wan, Jiayu |
| description | Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to
Salmonella typhimurium
(
S. typhimurium
), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of
S. typhimurium
from 10
2
to 10
8
CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria. |
| doi_str_mv | 10.1007/s00216-020-02928-7 |
| format | Article |
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Salmonella typhimurium
(
S. typhimurium
), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of
S. typhimurium
from 10
2
to 10
8
CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-020-02928-7</identifier><identifier>PMID: 32879993</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetaldehyde ; Acetic acid ; Aldehyde Dehydrogenase - metabolism ; Analytical Chemistry ; Animals ; Bacteria ; Biochemistry ; Biosensing Techniques - methods ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Colony Count, Microbial ; Detectors ; Enzymes ; Food Science ; Laboratory Medicine ; Limit of Detection ; Microscopy, Electron, Scanning ; Milk - microbiology ; Monitoring/Environmental Analysis ; Organic acids ; Research Paper ; Salmonella typhimurium - isolation & purification ; Signal Transduction</subject><ispartof>Analytical and bioanalytical chemistry, 2020-11, Vol.412 (28), p.7955-7962</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-c340fc738d7cb5eaebb72242ab313daf862406ce6f2d2ce9d4c066783f8ee32b3</citedby><cites>FETCH-LOGICAL-c451t-c340fc738d7cb5eaebb72242ab313daf862406ce6f2d2ce9d4c066783f8ee32b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-020-02928-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-020-02928-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32879993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Wenguang</creatorcontrib><creatorcontrib>Bu, Shengjun</creatorcontrib><creatorcontrib>Bai, Huasong</creatorcontrib><creatorcontrib>Ma, Chengyou</creatorcontrib><creatorcontrib>Ma, Li</creatorcontrib><creatorcontrib>Wei, Hongguo</creatorcontrib><creatorcontrib>Liu, Xiu</creatorcontrib><creatorcontrib>Li, Zehong</creatorcontrib><creatorcontrib>Wan, Jiayu</creatorcontrib><title>A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to
Salmonella typhimurium
(
S. typhimurium
), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of
S. typhimurium
from 10
2
to 10
8
CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria.</description><subject>Acetaldehyde</subject><subject>Acetic acid</subject><subject>Aldehyde Dehydrogenase - metabolism</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biosensing Techniques - methods</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colony Count, Microbial</subject><subject>Detectors</subject><subject>Enzymes</subject><subject>Food Science</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Microscopy, Electron, Scanning</subject><subject>Milk - microbiology</subject><subject>Monitoring/Environmental Analysis</subject><subject>Organic acids</subject><subject>Research Paper</subject><subject>Salmonella typhimurium - isolation & purification</subject><subject>Signal Transduction</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9rFjEQxoMotla_gAcJePGyNX92k-zxpVQrFLzoOWSTyTZlN3lNdoX32zfbrQVBJAyTyfyeIcyD0HtKLikh8nMhhFHREEZq9Ew18gU6p4KqhomOvHy-t-wMvSnlnhDaKSpeozPOlOz7np-j6YALxBKW8BvwENJWpIx9DQcL5DlEs4QUcfL4aJa7NEIMFg_G1mYweC0hjthMDu5ODvBjyhtkCuASxmimDSinssD8Fr3yZirw7ilfoJ9frn9c3TS3379-uzrcNrbt6NJY3hJvJVdO2qEDA8MgGWuZGTjlznglWEuEBeGZYxZ611oihFTcKwDOBn6BPu1zjzn9WqEseg7FwjSZCGktmrW872XHFavoxx0dzQQ6RJ-WbOyG64OoQNcztlGX_6DqcTAHmyL4UN__ErBdYHMqJYPXxxxmk0-aEr2Zp3fzdDVPP5qnZRV9ePr2OszgniV_3KoA34FSW3GErO_TmuuKy__GPgAdD6Yl</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Zhang, Wenguang</creator><creator>Bu, Shengjun</creator><creator>Bai, Huasong</creator><creator>Ma, Chengyou</creator><creator>Ma, Li</creator><creator>Wei, Hongguo</creator><creator>Liu, Xiu</creator><creator>Li, Zehong</creator><creator>Wan, Jiayu</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20201101</creationdate><title>A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system</title><author>Zhang, Wenguang ; Bu, Shengjun ; Bai, Huasong ; Ma, Chengyou ; Ma, Li ; Wei, Hongguo ; Liu, Xiu ; Li, Zehong ; Wan, Jiayu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-c340fc738d7cb5eaebb72242ab313daf862406ce6f2d2ce9d4c066783f8ee32b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acetaldehyde</topic><topic>Acetic acid</topic><topic>Aldehyde Dehydrogenase - metabolism</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biosensing Techniques - methods</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colony Count, Microbial</topic><topic>Detectors</topic><topic>Enzymes</topic><topic>Food Science</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Microscopy, Electron, Scanning</topic><topic>Milk - microbiology</topic><topic>Monitoring/Environmental Analysis</topic><topic>Organic acids</topic><topic>Research Paper</topic><topic>Salmonella typhimurium - isolation & purification</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Wenguang</creatorcontrib><creatorcontrib>Bu, Shengjun</creatorcontrib><creatorcontrib>Bai, Huasong</creatorcontrib><creatorcontrib>Ma, Chengyou</creatorcontrib><creatorcontrib>Ma, Li</creatorcontrib><creatorcontrib>Wei, Hongguo</creatorcontrib><creatorcontrib>Liu, Xiu</creatorcontrib><creatorcontrib>Li, Zehong</creatorcontrib><creatorcontrib>Wan, Jiayu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Wenguang</au><au>Bu, Shengjun</au><au>Bai, Huasong</au><au>Ma, Chengyou</au><au>Ma, Li</au><au>Wei, Hongguo</au><au>Liu, Xiu</au><au>Li, Zehong</au><au>Wan, Jiayu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>412</volume><issue>28</issue><spage>7955</spage><epage>7962</epage><pages>7955-7962</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Aldehyde dehydrogenase (ALDH) was first developed as an enzymatic signaling system of a biosensor for sensitive point-of-care detection of pathogenic bacteria. ALDH and specific aptamers to
Salmonella typhimurium
(
S. typhimurium
), as organic components, were embedded in organic-inorganic nanocomposites as a biosensor signal label, integrating the functions of signal amplification and target recognition. The biosensing mechanism is based on the fact that ALDH can catalyze rapid oxidation of acetaldehyde into acetic acid, resulting in pH change with portable pH meter readout. The altered pH exhibited a linear relationship with the logarithm of
S. typhimurium
from 10
2
to 10
8
CFU/mL and detection limit of 46 CFU/mL. Thus, the proposed biosensor has potential application in the diagnosis of pathogenic bacteria.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32879993</pmid><doi>10.1007/s00216-020-02928-7</doi><tpages>8</tpages></addata></record> |
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| subjects | Acetaldehyde Acetic acid Aldehyde Dehydrogenase - metabolism Analytical Chemistry Animals Bacteria Biochemistry Biosensing Techniques - methods Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Colony Count, Microbial Detectors Enzymes Food Science Laboratory Medicine Limit of Detection Microscopy, Electron, Scanning Milk - microbiology Monitoring/Environmental Analysis Organic acids Research Paper Salmonella typhimurium - isolation & purification Signal Transduction |
| title | A sensitive biosensor for determination of pathogenic bacteria using aldehyde dehydrogenase signaling system |
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