Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1
l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivat...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2020-09, Vol.68 (39), p.10678-10684 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10684 |
|---|---|
| container_issue | 39 |
| container_start_page | 10678 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 68 |
| creator | Zhang, Xia Gan, Min Li, Jingyun Li, Hui Su, Meicheng Tan, Dongfei Wang, Shaolei Jia, Man Zhang, Liguo Chen, Gang |
| description | l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the aryl hydrocarbon receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Trp to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity. |
| doi_str_mv | 10.1021/acs.jafc.0c03735 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2439634912</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2439634912</sourcerecordid><originalsourceid>FETCH-LOGICAL-a336t-57e15d073ab60d4cd17ee3e18fcd65c914db25de43f73abf1cac08c24fa510b13</originalsourceid><addsrcrecordid>eNp1kD1PwzAURS0EoqWwM6GMDKS8F8dJOqKqQKXyMZTZcmyHpkriYCeg_HtcWtiYfC2de6V3CLlEmCJEeCukm25FIacggaaUHZExsghChpgdkzF4JsxYgiNy5twWADKWwikZ0ShLEv8dk-dFo8y7bkzvgqWPlQ5eB9t_is4H0W2-xBAUxgZrO7SdaTeiCZ50J3JTla72UZWeVEE-BMtVvMRzclKIyumLwzshb_eL9fwxXL08LOd3q1BQmnQhSzUyBSkVeQIqlgpTranGrJAqYXKGscojpnRMix1ToBQSMhnFhWAIOdIJud7vttZ89Np1vC6d1FUlGu1P4VFMZwmNZxh5FPaotMY5qwve2rIWduAIfGeRe4t8Z5EfLPrK1WG9z2ut_gq_2jxwswd-qqa3jT_2_71vQCZ98Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2439634912</pqid></control><display><type>article</type><title>Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1</title><source>MEDLINE</source><source>ACS Publications</source><creator>Zhang, Xia ; Gan, Min ; Li, Jingyun ; Li, Hui ; Su, Meicheng ; Tan, Dongfei ; Wang, Shaolei ; Jia, Man ; Zhang, Liguo ; Chen, Gang</creator><creatorcontrib>Zhang, Xia ; Gan, Min ; Li, Jingyun ; Li, Hui ; Su, Meicheng ; Tan, Dongfei ; Wang, Shaolei ; Jia, Man ; Zhang, Liguo ; Chen, Gang</creatorcontrib><description>l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the aryl hydrocarbon receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Trp to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.0c03735</identifier><identifier>PMID: 32866000</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Bioactive Constituents, Metabolites, and Functions ; Dendritic Cells - metabolism ; Female ; Humans ; Indoleacetic Acids - metabolism ; Indoles - metabolism ; L-Amino Acid Oxidase - genetics ; L-Amino Acid Oxidase - metabolism ; Mice ; Mice, Inbred C57BL ; Pyruvic Acid - metabolism ; Tryptophan - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2020-09, Vol.68 (39), p.10678-10684</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-57e15d073ab60d4cd17ee3e18fcd65c914db25de43f73abf1cac08c24fa510b13</citedby><cites>FETCH-LOGICAL-a336t-57e15d073ab60d4cd17ee3e18fcd65c914db25de43f73abf1cac08c24fa510b13</cites><orcidid>0000-0002-0360-4091</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.0c03735$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.0c03735$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,778,782,2754,27059,27907,27908,56721,56771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32866000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xia</creatorcontrib><creatorcontrib>Gan, Min</creatorcontrib><creatorcontrib>Li, Jingyun</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Su, Meicheng</creatorcontrib><creatorcontrib>Tan, Dongfei</creatorcontrib><creatorcontrib>Wang, Shaolei</creatorcontrib><creatorcontrib>Jia, Man</creatorcontrib><creatorcontrib>Zhang, Liguo</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><title>Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the aryl hydrocarbon receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Trp to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity.</description><subject>Animals</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Dendritic Cells - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Indoles - metabolism</subject><subject>L-Amino Acid Oxidase - genetics</subject><subject>L-Amino Acid Oxidase - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Pyruvic Acid - metabolism</subject><subject>Tryptophan - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAURS0EoqWwM6GMDKS8F8dJOqKqQKXyMZTZcmyHpkriYCeg_HtcWtiYfC2de6V3CLlEmCJEeCukm25FIacggaaUHZExsghChpgdkzF4JsxYgiNy5twWADKWwikZ0ShLEv8dk-dFo8y7bkzvgqWPlQ5eB9t_is4H0W2-xBAUxgZrO7SdaTeiCZ50J3JTla72UZWeVEE-BMtVvMRzclKIyumLwzshb_eL9fwxXL08LOd3q1BQmnQhSzUyBSkVeQIqlgpTranGrJAqYXKGscojpnRMix1ToBQSMhnFhWAIOdIJud7vttZ89Np1vC6d1FUlGu1P4VFMZwmNZxh5FPaotMY5qwve2rIWduAIfGeRe4t8Z5EfLPrK1WG9z2ut_gq_2jxwswd-qqa3jT_2_71vQCZ98Q</recordid><startdate>20200930</startdate><enddate>20200930</enddate><creator>Zhang, Xia</creator><creator>Gan, Min</creator><creator>Li, Jingyun</creator><creator>Li, Hui</creator><creator>Su, Meicheng</creator><creator>Tan, Dongfei</creator><creator>Wang, Shaolei</creator><creator>Jia, Man</creator><creator>Zhang, Liguo</creator><creator>Chen, Gang</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0360-4091</orcidid></search><sort><creationdate>20200930</creationdate><title>Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1</title><author>Zhang, Xia ; Gan, Min ; Li, Jingyun ; Li, Hui ; Su, Meicheng ; Tan, Dongfei ; Wang, Shaolei ; Jia, Man ; Zhang, Liguo ; Chen, Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-57e15d073ab60d4cd17ee3e18fcd65c914db25de43f73abf1cac08c24fa510b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Dendritic Cells - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Indoles - metabolism</topic><topic>L-Amino Acid Oxidase - genetics</topic><topic>L-Amino Acid Oxidase - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Pyruvic Acid - metabolism</topic><topic>Tryptophan - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xia</creatorcontrib><creatorcontrib>Gan, Min</creatorcontrib><creatorcontrib>Li, Jingyun</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Su, Meicheng</creatorcontrib><creatorcontrib>Tan, Dongfei</creatorcontrib><creatorcontrib>Wang, Shaolei</creatorcontrib><creatorcontrib>Jia, Man</creatorcontrib><creatorcontrib>Zhang, Liguo</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xia</au><au>Gan, Min</au><au>Li, Jingyun</au><au>Li, Hui</au><au>Su, Meicheng</au><au>Tan, Dongfei</au><au>Wang, Shaolei</au><au>Jia, Man</au><au>Zhang, Liguo</au><au>Chen, Gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2020-09-30</date><risdate>2020</risdate><volume>68</volume><issue>39</issue><spage>10678</spage><epage>10684</epage><pages>10678-10684</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the aryl hydrocarbon receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Trp to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>32866000</pmid><doi>10.1021/acs.jafc.0c03735</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0360-4091</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2020-09, Vol.68 (39), p.10678-10684 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2439634912 |
| source | MEDLINE; ACS Publications |
| subjects | Animals Bioactive Constituents, Metabolites, and Functions Dendritic Cells - metabolism Female Humans Indoleacetic Acids - metabolism Indoles - metabolism L-Amino Acid Oxidase - genetics L-Amino Acid Oxidase - metabolism Mice Mice, Inbred C57BL Pyruvic Acid - metabolism Tryptophan - metabolism |
| title | Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T19%3A11%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Endogenous%20Indole%20Pyruvate%20Pathway%20for%20Tryptophan%20Metabolism%20Mediated%20by%20IL4I1&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Zhang,%20Xia&rft.date=2020-09-30&rft.volume=68&rft.issue=39&rft.spage=10678&rft.epage=10684&rft.pages=10678-10684&rft.issn=0021-8561&rft.eissn=1520-5118&rft_id=info:doi/10.1021/acs.jafc.0c03735&rft_dat=%3Cproquest_cross%3E2439634912%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2439634912&rft_id=info:pmid/32866000&rfr_iscdi=true |