Peptides from Extruded Lupin (Lupinus albus L.) Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells
In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characte...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2020-10, Vol.68 (42), p.11702-11709 |
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| creator | Gao, Yue Zhang, Xuna Ren, Guixing Wu, Caie Qin, Peiyou Yao, Yang |
| description | In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characterized after UPLC–MS/MS, was screened out by macrophage inflammatory cytokine production assay. RNA-sequencing analysis was performed to explore the mechanisms underlying the anti-inflammatory activity associated with this peptide. The results indicated that lupin peptides effectively inhibited the lipopolysaccharide-induced overproduction of proinflammatory mediators. IQD inhibited the production of tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and monocyte chemoattractant protein-1 by 51.20, 38.52, 44.70, and 40.43%, respectively. RNA-sequencing results showed that IQD inhibited the inflammatory response by regulating the gene expression of the p38 mitogen-activated protein kinase pathway and inhibiting downstream inflammatory cytokines. These bioactive peptides may be used to develop new ingredients for anti-inflammatory nutritional supplements. |
| doi_str_mv | 10.1021/acs.jafc.0c02476 |
| format | Article |
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Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells</title><source>ACS Publications</source><source>MEDLINE</source><creator>Gao, Yue ; Zhang, Xuna ; Ren, Guixing ; Wu, Caie ; Qin, Peiyou ; Yao, Yang</creator><creatorcontrib>Gao, Yue ; Zhang, Xuna ; Ren, Guixing ; Wu, Caie ; Qin, Peiyou ; Yao, Yang</creatorcontrib><description>In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characterized after UPLC–MS/MS, was screened out by macrophage inflammatory cytokine production assay. RNA-sequencing analysis was performed to explore the mechanisms underlying the anti-inflammatory activity associated with this peptide. The results indicated that lupin peptides effectively inhibited the lipopolysaccharide-induced overproduction of proinflammatory mediators. IQD inhibited the production of tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and monocyte chemoattractant protein-1 by 51.20, 38.52, 44.70, and 40.43%, respectively. RNA-sequencing results showed that IQD inhibited the inflammatory response by regulating the gene expression of the p38 mitogen-activated protein kinase pathway and inhibiting downstream inflammatory cytokines. These bioactive peptides may be used to develop new ingredients for anti-inflammatory nutritional supplements.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.0c02476</identifier><identifier>PMID: 32869636</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Anti-Inflammatory Agents - pharmacology ; Bioactive Constituents, Metabolites, and Functions ; Inflammation - genetics ; Inflammation - immunology ; Lupinus - chemistry ; Macrophages - drug effects ; Macrophages - immunology ; Mice ; p38 Mitogen-Activated Protein Kinases - genetics ; p38 Mitogen-Activated Protein Kinases - immunology ; Peptides - pharmacology ; Plant Proteins - chemistry ; RAW 264.7 Cells ; Seeds - chemistry ; Tandem Mass Spectrometry</subject><ispartof>Journal of agricultural and food chemistry, 2020-10, Vol.68 (42), p.11702-11709</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-191387c27b2e9c42cff81820312bc6eee2132a7af1c255820f46dfb296c6c6cb3</citedby><cites>FETCH-LOGICAL-a336t-191387c27b2e9c42cff81820312bc6eee2132a7af1c255820f46dfb296c6c6cb3</cites><orcidid>0000-0002-8672-408X ; 0000-0001-7733-930X ; 0000-0002-1126-5464 ; 0000-0002-1306-7349</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.0c02476$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.0c02476$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32869636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Yue</creatorcontrib><creatorcontrib>Zhang, Xuna</creatorcontrib><creatorcontrib>Ren, Guixing</creatorcontrib><creatorcontrib>Wu, Caie</creatorcontrib><creatorcontrib>Qin, Peiyou</creatorcontrib><creatorcontrib>Yao, Yang</creatorcontrib><title>Peptides from Extruded Lupin (Lupinus albus L.) Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characterized after UPLC–MS/MS, was screened out by macrophage inflammatory cytokine production assay. RNA-sequencing analysis was performed to explore the mechanisms underlying the anti-inflammatory activity associated with this peptide. The results indicated that lupin peptides effectively inhibited the lipopolysaccharide-induced overproduction of proinflammatory mediators. IQD inhibited the production of tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and monocyte chemoattractant protein-1 by 51.20, 38.52, 44.70, and 40.43%, respectively. RNA-sequencing results showed that IQD inhibited the inflammatory response by regulating the gene expression of the p38 mitogen-activated protein kinase pathway and inhibiting downstream inflammatory cytokines. These bioactive peptides may be used to develop new ingredients for anti-inflammatory nutritional supplements.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Inflammation - genetics</subject><subject>Inflammation - immunology</subject><subject>Lupinus - chemistry</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Mice</subject><subject>p38 Mitogen-Activated Protein Kinases - genetics</subject><subject>p38 Mitogen-Activated Protein Kinases - immunology</subject><subject>Peptides - pharmacology</subject><subject>Plant Proteins - chemistry</subject><subject>RAW 264.7 Cells</subject><subject>Seeds - chemistry</subject><subject>Tandem Mass Spectrometry</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtP3DAUhS1EBVPonlV1l1QiwY_EcZajEaWogxhRqi4jx7EhKC_8AOZP9DfXwwzdoSvdu_B3zrXuQeiE4JRgSs6lcumjNCrFCtOs4HtoRnKKk5wQsY9mODKJyDk5RJ-de8QYi7zAB-iQUcFLzvgM_V3pybeNdmDs2MPFq7eh0Q0sw9QOcPo2ggPZ1bEv029wq-9DJ72Gq8F0su-lH-0a5sq3z61fw3MrwT9omJiA6_nqJ_xq7wfZwZ2Vg2tCxMYBVtI_vMg1xA238z9AeZYWsNBd547RJyM7p7_s5hH6_f3ibvEjWd5cXi3my0Qyxn1CSsJEoWhRU12qjCpjBBEUM0JrxbXWlDAqC2mIonkeH0zGG1PTkqtN1ewInW59Jzs-Be181bdOxR_IQY_BVTRjJac5F0VE8RZVdnTOalNNtu2lXVcEV5sUqphCtUmh2qUQJV937qHudfNf8H72CJxtgTfpGGw8kfvY7x8dPZJq</recordid><startdate>20201021</startdate><enddate>20201021</enddate><creator>Gao, Yue</creator><creator>Zhang, Xuna</creator><creator>Ren, Guixing</creator><creator>Wu, Caie</creator><creator>Qin, Peiyou</creator><creator>Yao, Yang</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8672-408X</orcidid><orcidid>https://orcid.org/0000-0001-7733-930X</orcidid><orcidid>https://orcid.org/0000-0002-1126-5464</orcidid><orcidid>https://orcid.org/0000-0002-1306-7349</orcidid></search><sort><creationdate>20201021</creationdate><title>Peptides from Extruded Lupin (Lupinus albus L.) Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells</title><author>Gao, Yue ; Zhang, Xuna ; Ren, Guixing ; Wu, Caie ; Qin, Peiyou ; Yao, Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-191387c27b2e9c42cff81820312bc6eee2132a7af1c255820f46dfb296c6c6cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Inflammation - genetics</topic><topic>Inflammation - immunology</topic><topic>Lupinus - chemistry</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Mice</topic><topic>p38 Mitogen-Activated Protein Kinases - genetics</topic><topic>p38 Mitogen-Activated Protein Kinases - immunology</topic><topic>Peptides - pharmacology</topic><topic>Plant Proteins - chemistry</topic><topic>RAW 264.7 Cells</topic><topic>Seeds - chemistry</topic><topic>Tandem Mass Spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Yue</creatorcontrib><creatorcontrib>Zhang, Xuna</creatorcontrib><creatorcontrib>Ren, Guixing</creatorcontrib><creatorcontrib>Wu, Caie</creatorcontrib><creatorcontrib>Qin, Peiyou</creatorcontrib><creatorcontrib>Yao, Yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Yue</au><au>Zhang, Xuna</au><au>Ren, Guixing</au><au>Wu, Caie</au><au>Qin, Peiyou</au><au>Yao, Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peptides from Extruded Lupin (Lupinus albus L.) Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2020-10-21</date><risdate>2020</risdate><volume>68</volume><issue>42</issue><spage>11702</spage><epage>11709</epage><pages>11702-11709</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characterized after UPLC–MS/MS, was screened out by macrophage inflammatory cytokine production assay. RNA-sequencing analysis was performed to explore the mechanisms underlying the anti-inflammatory activity associated with this peptide. The results indicated that lupin peptides effectively inhibited the lipopolysaccharide-induced overproduction of proinflammatory mediators. IQD inhibited the production of tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and monocyte chemoattractant protein-1 by 51.20, 38.52, 44.70, and 40.43%, respectively. RNA-sequencing results showed that IQD inhibited the inflammatory response by regulating the gene expression of the p38 mitogen-activated protein kinase pathway and inhibiting downstream inflammatory cytokines. These bioactive peptides may be used to develop new ingredients for anti-inflammatory nutritional supplements.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>32869636</pmid><doi>10.1021/acs.jafc.0c02476</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8672-408X</orcidid><orcidid>https://orcid.org/0000-0001-7733-930X</orcidid><orcidid>https://orcid.org/0000-0002-1126-5464</orcidid><orcidid>https://orcid.org/0000-0002-1306-7349</orcidid></addata></record> |
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| subjects | Animals Anti-Inflammatory Agents - pharmacology Bioactive Constituents, Metabolites, and Functions Inflammation - genetics Inflammation - immunology Lupinus - chemistry Macrophages - drug effects Macrophages - immunology Mice p38 Mitogen-Activated Protein Kinases - genetics p38 Mitogen-Activated Protein Kinases - immunology Peptides - pharmacology Plant Proteins - chemistry RAW 264.7 Cells Seeds - chemistry Tandem Mass Spectrometry |
| title | Peptides from Extruded Lupin (Lupinus albus L.) Regulate Inflammatory Activity via the p38 MAPK Signal Transduction Pathway in RAW 264.7 Cells |
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