Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage‐dependent calcium channels
Aim We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur‐containing foods such as garlic on rat uterine activity. Methods Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague–Dawley rats (250–300 g) in es...
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| Veröffentlicht in: | The journal of obstetrics and gynaecology research 2021-01, Vol.47 (1), p.88-95 |
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| container_title | The journal of obstetrics and gynaecology research |
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| creator | Efe, Oğuzhan E. Lux, K. Michael Emre Aydingöz, Selda Tuncer, Meral |
| description | Aim
We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur‐containing foods such as garlic on rat uterine activity.
Methods
Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague–Dawley rats (250–300 g) in estrus stage of estrous cycle were studied in isolated organ baths containing Krebs–Henseleit solution.
Results
Diallyl sulfide (10−8–10−6 M) caused a concentration‐dependent relaxation on KCl (60 mM)‐induced contractions and inhibited spontaneous peristaltic activity of uterine strips (P 0.05): nitric oxide synthase inhibitor L‐NAME (10−4 M), hydrogen sulfide‐producing enzymes cystation β synthase and cystation γ‐lyase inhibitors, aminooxyacetic acid (10−4 M) and propargylglycine (10−3 M) and nonselective cyclooxygenase inhibitor indomethacin (10−4 M). However, in calcium‐free Krebs solution containing high KCl (30 mM), DAS significantly inhibited CaCl2 (10−5–10−2 M)‐induced uterine contractions in a concentration‐dependent manner (P |
| doi_str_mv | 10.1111/jog.14421 |
| format | Article |
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We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur‐containing foods such as garlic on rat uterine activity.
Methods
Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague–Dawley rats (250–300 g) in estrus stage of estrous cycle were studied in isolated organ baths containing Krebs–Henseleit solution.
Results
Diallyl sulfide (10−8–10−6 M) caused a concentration‐dependent relaxation on KCl (60 mM)‐induced contractions and inhibited spontaneous peristaltic activity of uterine strips (P < 0.05). None of the following antagonists significantly changed the inhibitory effect of DAS on both KCl‐precontracted uterine strips and spontaneous peristaltic activity of the uterus (P > 0.05): nitric oxide synthase inhibitor L‐NAME (10−4 M), hydrogen sulfide‐producing enzymes cystation β synthase and cystation γ‐lyase inhibitors, aminooxyacetic acid (10−4 M) and propargylglycine (10−3 M) and nonselective cyclooxygenase inhibitor indomethacin (10−4 M). However, in calcium‐free Krebs solution containing high KCl (30 mM), DAS significantly inhibited CaCl2 (10−5–10−2 M)‐induced uterine contractions in a concentration‐dependent manner (P < 0.05).
Conclusion
Diallyl sulfide has a relaxing effect on KCl‐contracted rat uterus strips and an inhibitory effect on spontaneous uterine activity, possibly by decreasing the calcium influx into the cytoplasm of uterine smooth muscle cells.</description><identifier>ISSN: 1341-8076</identifier><identifier>EISSN: 1447-0756</identifier><identifier>DOI: 10.1111/jog.14421</identifier><identifier>PMID: 32830389</identifier><language>eng</language><publisher>Kyoto, Japan: John Wiley & Sons Australia, Ltd</publisher><subject>Antagonists ; Calcium channels ; Calcium channels (voltage-gated) ; Calcium chloride ; Calcium influx ; Cytoplasm ; diallyl sulfide ; Estrus cycle ; garlic ; Hydrogen sulfide ; Indomethacin ; Muscle contraction ; Nitric oxide ; Nitric-oxide synthase ; organ bath ; Potassium chloride ; Prostaglandin endoperoxide synthase ; rat ; Smooth muscle ; Sulfur ; Uterus</subject><ispartof>The journal of obstetrics and gynaecology research, 2021-01, Vol.47 (1), p.88-95</ispartof><rights>2020 Japan Society of Obstetrics and Gynecology</rights><rights>2020 Japan Society of Obstetrics and Gynecology.</rights><rights>2021 Japan Society of Obstetrics and Gynecology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3771-d11b24cc4f06f9e199d3e4c095cb9cd54df3627b3b331ea388da20687baaf9163</citedby><cites>FETCH-LOGICAL-c3771-d11b24cc4f06f9e199d3e4c095cb9cd54df3627b3b331ea388da20687baaf9163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjog.14421$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjog.14421$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32830389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Efe, Oğuzhan E.</creatorcontrib><creatorcontrib>Lux, K. Michael</creatorcontrib><creatorcontrib>Emre Aydingöz, Selda</creatorcontrib><creatorcontrib>Tuncer, Meral</creatorcontrib><title>Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage‐dependent calcium channels</title><title>The journal of obstetrics and gynaecology research</title><addtitle>J Obstet Gynaecol Res</addtitle><description>Aim
We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur‐containing foods such as garlic on rat uterine activity.
Methods
Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague–Dawley rats (250–300 g) in estrus stage of estrous cycle were studied in isolated organ baths containing Krebs–Henseleit solution.
Results
Diallyl sulfide (10−8–10−6 M) caused a concentration‐dependent relaxation on KCl (60 mM)‐induced contractions and inhibited spontaneous peristaltic activity of uterine strips (P < 0.05). None of the following antagonists significantly changed the inhibitory effect of DAS on both KCl‐precontracted uterine strips and spontaneous peristaltic activity of the uterus (P > 0.05): nitric oxide synthase inhibitor L‐NAME (10−4 M), hydrogen sulfide‐producing enzymes cystation β synthase and cystation γ‐lyase inhibitors, aminooxyacetic acid (10−4 M) and propargylglycine (10−3 M) and nonselective cyclooxygenase inhibitor indomethacin (10−4 M). However, in calcium‐free Krebs solution containing high KCl (30 mM), DAS significantly inhibited CaCl2 (10−5–10−2 M)‐induced uterine contractions in a concentration‐dependent manner (P < 0.05).
Conclusion
Diallyl sulfide has a relaxing effect on KCl‐contracted rat uterus strips and an inhibitory effect on spontaneous uterine activity, possibly by decreasing the calcium influx into the cytoplasm of uterine smooth muscle cells.</description><subject>Antagonists</subject><subject>Calcium channels</subject><subject>Calcium channels (voltage-gated)</subject><subject>Calcium chloride</subject><subject>Calcium influx</subject><subject>Cytoplasm</subject><subject>diallyl sulfide</subject><subject>Estrus cycle</subject><subject>garlic</subject><subject>Hydrogen sulfide</subject><subject>Indomethacin</subject><subject>Muscle contraction</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>organ bath</subject><subject>Potassium chloride</subject><subject>Prostaglandin endoperoxide synthase</subject><subject>rat</subject><subject>Smooth muscle</subject><subject>Sulfur</subject><subject>Uterus</subject><issn>1341-8076</issn><issn>1447-0756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp10c1u3CAQB3BUNcr3oS9QIfWSHJwwhrVNb1GU5kORIlXN2cIwbL3CsAE7yd7yCH3GPknZbNJDpXIZQL_5Cw2EfAJ2AnmdLsL8BIQo4QPZzbUuWD2rPuY9F1A0rK52yF5KC8agltBskx1eNpzxRu6Sh-_o1LPyI0VrUY80WGp65dzK0TQ52xukwVMf_DLi3K9hVCOdRoxT-kqv_WNwjzigf-3Mh1HN8ffLL4NL9GZ9rZXT_TRQ_VN5jy4dkC2rXMLDt7pP7r9d_Di_Km7vLq_Pz24LzesaCgPQlUJrYVllJYKUhqPQTM50J7WZCWN5VdYd7zgHVLxpjCpZ1dSdUlZCxffJ0SZ3GcPDhGlshz5pdE55DFNqS8GzBpBlpl_-oYswRZ9fl1UeJTSzSmR1vFE6hpQi2nYZ-0HFVQusXf9D7pq3r_-Q7ee3xKkb0PyV74PP4HQDnnqHq_8ntTd3l5vIP3n3k-o</recordid><startdate>202101</startdate><enddate>202101</enddate><creator>Efe, Oğuzhan E.</creator><creator>Lux, K. Michael</creator><creator>Emre Aydingöz, Selda</creator><creator>Tuncer, Meral</creator><general>John Wiley & Sons Australia, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>202101</creationdate><title>Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage‐dependent calcium channels</title><author>Efe, Oğuzhan E. ; Lux, K. Michael ; Emre Aydingöz, Selda ; Tuncer, Meral</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3771-d11b24cc4f06f9e199d3e4c095cb9cd54df3627b3b331ea388da20687baaf9163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antagonists</topic><topic>Calcium channels</topic><topic>Calcium channels (voltage-gated)</topic><topic>Calcium chloride</topic><topic>Calcium influx</topic><topic>Cytoplasm</topic><topic>diallyl sulfide</topic><topic>Estrus cycle</topic><topic>garlic</topic><topic>Hydrogen sulfide</topic><topic>Indomethacin</topic><topic>Muscle contraction</topic><topic>Nitric oxide</topic><topic>Nitric-oxide synthase</topic><topic>organ bath</topic><topic>Potassium chloride</topic><topic>Prostaglandin endoperoxide synthase</topic><topic>rat</topic><topic>Smooth muscle</topic><topic>Sulfur</topic><topic>Uterus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Efe, Oğuzhan E.</creatorcontrib><creatorcontrib>Lux, K. Michael</creatorcontrib><creatorcontrib>Emre Aydingöz, Selda</creatorcontrib><creatorcontrib>Tuncer, Meral</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of obstetrics and gynaecology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Efe, Oğuzhan E.</au><au>Lux, K. Michael</au><au>Emre Aydingöz, Selda</au><au>Tuncer, Meral</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage‐dependent calcium channels</atitle><jtitle>The journal of obstetrics and gynaecology research</jtitle><addtitle>J Obstet Gynaecol Res</addtitle><date>2021-01</date><risdate>2021</risdate><volume>47</volume><issue>1</issue><spage>88</spage><epage>95</epage><pages>88-95</pages><issn>1341-8076</issn><eissn>1447-0756</eissn><abstract>Aim
We aimed to determine the effect and mechanism of action of diallyl sulfide (DAS), an active component of sulfur‐containing foods such as garlic on rat uterine activity.
Methods
Isometric tension changes in longitudinal uterine strips obtained from 20 female Sprague–Dawley rats (250–300 g) in estrus stage of estrous cycle were studied in isolated organ baths containing Krebs–Henseleit solution.
Results
Diallyl sulfide (10−8–10−6 M) caused a concentration‐dependent relaxation on KCl (60 mM)‐induced contractions and inhibited spontaneous peristaltic activity of uterine strips (P < 0.05). None of the following antagonists significantly changed the inhibitory effect of DAS on both KCl‐precontracted uterine strips and spontaneous peristaltic activity of the uterus (P > 0.05): nitric oxide synthase inhibitor L‐NAME (10−4 M), hydrogen sulfide‐producing enzymes cystation β synthase and cystation γ‐lyase inhibitors, aminooxyacetic acid (10−4 M) and propargylglycine (10−3 M) and nonselective cyclooxygenase inhibitor indomethacin (10−4 M). However, in calcium‐free Krebs solution containing high KCl (30 mM), DAS significantly inhibited CaCl2 (10−5–10−2 M)‐induced uterine contractions in a concentration‐dependent manner (P < 0.05).
Conclusion
Diallyl sulfide has a relaxing effect on KCl‐contracted rat uterus strips and an inhibitory effect on spontaneous uterine activity, possibly by decreasing the calcium influx into the cytoplasm of uterine smooth muscle cells.</abstract><cop>Kyoto, Japan</cop><pub>John Wiley & Sons Australia, Ltd</pub><pmid>32830389</pmid><doi>10.1111/jog.14421</doi><tpages>8</tpages></addata></record> |
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| subjects | Antagonists Calcium channels Calcium channels (voltage-gated) Calcium chloride Calcium influx Cytoplasm diallyl sulfide Estrus cycle garlic Hydrogen sulfide Indomethacin Muscle contraction Nitric oxide Nitric-oxide synthase organ bath Potassium chloride Prostaglandin endoperoxide synthase rat Smooth muscle Sulfur Uterus |
| title | Relaxant effect of diallyl sulfide on nonpregnant rat uterus: Involvement of voltage‐dependent calcium channels |
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