Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects
Background Infection by Adenovirus 36 (Ad-36) has been associated with adipogenesis using cell and animal models, and a high risk of developing obesity has been reported in Ad-36-seropositive individuals. However, molecular mechanisms involved in the maintenance over the years of adipogenesis associ...
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| Veröffentlicht in: | International Journal of Obesity 2020-11, Vol.44 (11), p.2303-2312 |
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| creator | Manríquez, Víctor Gutierrez, Alvaro Morales, Alexis Brito, Roberto Pavez, Monica Sapunar, Jorge Fonseca, Luis Molina, Víctor Ortiz, Eugenia Barra, Maria Ines Reimer, Camila Charles, Maria Schneider, Constance Cerda, Alvaro |
| description | Background
Infection by Adenovirus 36 (Ad-36) has been associated with adipogenesis using cell and animal models, and a high risk of developing obesity has been reported in Ad-36-seropositive individuals. However, molecular mechanisms involved in the maintenance over the years of adipogenesis associated with Ad-36 has not been investigated in human adipose tissue. Epigenetic mechanisms, such as micro-RNAs (miRNAs) that regulate gene expression at the post-transcriptional level, have shown an important role in the development and maintenance of metabolic diseases.
Aim
This study investigated the expression of miRNA associated with the adipogenic process in visceral adipose tissue from obese individuals according to Ad-36 serology.
Methods
Obese individuals were separated according to their status of Ad-36 serology in seropositive (Ad-36 (+);
n
= 29) and seronegative (Ad-36 (−);
n
= 28) groups. Additionally, a group of lean controls (
n
= 17) was selected to compare with obese individuals. Biopsies of visceral adipose tissue were obtained to evaluate miRNA and gene expression. The study of Ad-36 serology was carried out by ELISA. The expression of pro-adipogenic (miR-17 and miR-210) and anti-adipogenic (miR-155, miR-130 and miR-27a) miRNAs was evaluated using Taqman advanced miRNA assays by qPCR. The expression of adipogenes encoding
LEP
,
ADIPOQ
, and
PPARγ
was evaluated by Taqman predesigned assays through qPCR.
Results
The obese group had higher
LEP
(
p
|
| doi_str_mv | 10.1038/s41366-020-00654-9 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2436396911</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A639118050</galeid><sourcerecordid>A639118050</sourcerecordid><originalsourceid>FETCH-LOGICAL-c539t-4c45788635de51c5650db24d922815c97322780073fb024434382b3019e074f73</originalsourceid><addsrcrecordid>eNp9kltr3DAQhUVpabZp_0AfiqFQ-uJ0dLcfl9BLILRQ2mdhy-NdLV7L1dgh-fdVsrk0pRQ9CEnfOcyMDmOvOZxwkNUHUlwaU4KAEsBoVdZP2Iora0qtavuUrUCCLUEbfcReEO0AQGsQz9mRFJUwtZUrhmdjPyw4eixiXzQdjvEipIUKaQrCFKdIYQ4XYb4q4ljMWyzwckpIFPLxRhGmuMEx-GIffIrfv66pCPmpRcKClnaHfqaX7FnfDISvbvdj9vPTxx-nX8rzb5_PTtfnpdeynkvllbZVZaTuUHOfC4euFaqrhai49rlgIWwFYGXfglBKKlmJVgKvEazqrTxm7w--U4q_FqTZ7QN5HIZmxLiQE0oaWZua84y-_QvdxSWNubpMWa5qYUT1QG2aAV0Y-zinxl-bunV24rwCDZk6-QeVV4d5KHHEPuT7R4J3fwi22AzzluKwzHmq9BgUBzCPlihh76YU9k26chzcdQjcIQQuh8DdhMDVWfTmtrWl3WN3L7n79QzIA0D5adxgeuj9P7a_AZq9uDI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2471492628</pqid></control><display><type>article</type><title>Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects</title><source>Nature</source><source>Alma/SFX Local Collection</source><creator>Manríquez, Víctor ; Gutierrez, Alvaro ; Morales, Alexis ; Brito, Roberto ; Pavez, Monica ; Sapunar, Jorge ; Fonseca, Luis ; Molina, Víctor ; Ortiz, Eugenia ; Barra, Maria Ines ; Reimer, Camila ; Charles, Maria ; Schneider, Constance ; Cerda, Alvaro</creator><creatorcontrib>Manríquez, Víctor ; Gutierrez, Alvaro ; Morales, Alexis ; Brito, Roberto ; Pavez, Monica ; Sapunar, Jorge ; Fonseca, Luis ; Molina, Víctor ; Ortiz, Eugenia ; Barra, Maria Ines ; Reimer, Camila ; Charles, Maria ; Schneider, Constance ; Cerda, Alvaro</creatorcontrib><description>Background
Infection by Adenovirus 36 (Ad-36) has been associated with adipogenesis using cell and animal models, and a high risk of developing obesity has been reported in Ad-36-seropositive individuals. However, molecular mechanisms involved in the maintenance over the years of adipogenesis associated with Ad-36 has not been investigated in human adipose tissue. Epigenetic mechanisms, such as micro-RNAs (miRNAs) that regulate gene expression at the post-transcriptional level, have shown an important role in the development and maintenance of metabolic diseases.
Aim
This study investigated the expression of miRNA associated with the adipogenic process in visceral adipose tissue from obese individuals according to Ad-36 serology.
Methods
Obese individuals were separated according to their status of Ad-36 serology in seropositive (Ad-36 (+);
n
= 29) and seronegative (Ad-36 (−);
n
= 28) groups. Additionally, a group of lean controls (
n
= 17) was selected to compare with obese individuals. Biopsies of visceral adipose tissue were obtained to evaluate miRNA and gene expression. The study of Ad-36 serology was carried out by ELISA. The expression of pro-adipogenic (miR-17 and miR-210) and anti-adipogenic (miR-155, miR-130 and miR-27a) miRNAs was evaluated using Taqman advanced miRNA assays by qPCR. The expression of adipogenes encoding
LEP
,
ADIPOQ
, and
PPARγ
was evaluated by Taqman predesigned assays through qPCR.
Results
The obese group had higher
LEP
(
p
< 0.001) and
PPARγ
(
p
= 0.016) expression and lower
ADIPOQ
expression (
p
= 0.017), and also had higher expression of miR-210 (
p
= 0.039), whereas lower expression of miR-155 (
p
= 0.019) and miR-27a (
p
= 0.028) as compared to lean controls. Higher
PPARγ
expression (
p
= 0.008), but no influence on
LEP
or
ADIPOQ
expression was observed in Ad-36 (+) group. Those seropositive individuals also had higher expression of the miR-17 (
p
= 0.028) and lower levels of miR-155 (
p
= 0.031) in adipose tissue as compared to seronegative subjects.
Conclusions
Individuals with previous infection by Ad-36 had higher expression of the pro-adipogenic miR-17 and lower expression of the anti-adipogenic miR-155, which could lead to an increased adipogenic status by positively modulating
PPARγ
expression in adipose tissue from obese subjects.</description><identifier>ISSN: 0307-0565</identifier><identifier>EISSN: 1476-5497</identifier><identifier>DOI: 10.1038/s41366-020-00654-9</identifier><identifier>PMID: 32826973</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/39 ; 38/77 ; 692/499 ; 692/699/2743/393 ; 82/1 ; Adenoviruses ; Adipogenesis ; Adipose tissue ; Adipose tissues ; Advertising executives ; Animal models ; Cell culture ; Development and progression ; Enzyme-linked immunosorbent assay ; Epidemiology ; Epigenetic inheritance ; Epigenetics ; Gene expression ; Genes ; Genetic aspects ; Genetic research ; Genetic transcription ; Health Promotion and Disease Prevention ; Internal Medicine ; Maintenance ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Metabolic disorders ; MicroRNA ; MicroRNAs ; miRNA ; Molecular modelling ; Obesity ; Post-transcription ; Public Health ; Ribonucleic acid ; RNA ; Serology</subject><ispartof>International Journal of Obesity, 2020-11, Vol.44 (11), p.2303-2312</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020</rights><rights>COPYRIGHT 2020 Nature Publishing Group</rights><rights>The Author(s), under exclusive licence to Springer Nature Limited 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-4c45788635de51c5650db24d922815c97322780073fb024434382b3019e074f73</citedby><cites>FETCH-LOGICAL-c539t-4c45788635de51c5650db24d922815c97322780073fb024434382b3019e074f73</cites><orcidid>0000-0003-3428-8332</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32826973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manríquez, Víctor</creatorcontrib><creatorcontrib>Gutierrez, Alvaro</creatorcontrib><creatorcontrib>Morales, Alexis</creatorcontrib><creatorcontrib>Brito, Roberto</creatorcontrib><creatorcontrib>Pavez, Monica</creatorcontrib><creatorcontrib>Sapunar, Jorge</creatorcontrib><creatorcontrib>Fonseca, Luis</creatorcontrib><creatorcontrib>Molina, Víctor</creatorcontrib><creatorcontrib>Ortiz, Eugenia</creatorcontrib><creatorcontrib>Barra, Maria Ines</creatorcontrib><creatorcontrib>Reimer, Camila</creatorcontrib><creatorcontrib>Charles, Maria</creatorcontrib><creatorcontrib>Schneider, Constance</creatorcontrib><creatorcontrib>Cerda, Alvaro</creatorcontrib><title>Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects</title><title>International Journal of Obesity</title><addtitle>Int J Obes</addtitle><addtitle>Int J Obes (Lond)</addtitle><description>Background
Infection by Adenovirus 36 (Ad-36) has been associated with adipogenesis using cell and animal models, and a high risk of developing obesity has been reported in Ad-36-seropositive individuals. However, molecular mechanisms involved in the maintenance over the years of adipogenesis associated with Ad-36 has not been investigated in human adipose tissue. Epigenetic mechanisms, such as micro-RNAs (miRNAs) that regulate gene expression at the post-transcriptional level, have shown an important role in the development and maintenance of metabolic diseases.
Aim
This study investigated the expression of miRNA associated with the adipogenic process in visceral adipose tissue from obese individuals according to Ad-36 serology.
Methods
Obese individuals were separated according to their status of Ad-36 serology in seropositive (Ad-36 (+);
n
= 29) and seronegative (Ad-36 (−);
n
= 28) groups. Additionally, a group of lean controls (
n
= 17) was selected to compare with obese individuals. Biopsies of visceral adipose tissue were obtained to evaluate miRNA and gene expression. The study of Ad-36 serology was carried out by ELISA. The expression of pro-adipogenic (miR-17 and miR-210) and anti-adipogenic (miR-155, miR-130 and miR-27a) miRNAs was evaluated using Taqman advanced miRNA assays by qPCR. The expression of adipogenes encoding
LEP
,
ADIPOQ
, and
PPARγ
was evaluated by Taqman predesigned assays through qPCR.
Results
The obese group had higher
LEP
(
p
< 0.001) and
PPARγ
(
p
= 0.016) expression and lower
ADIPOQ
expression (
p
= 0.017), and also had higher expression of miR-210 (
p
= 0.039), whereas lower expression of miR-155 (
p
= 0.019) and miR-27a (
p
= 0.028) as compared to lean controls. Higher
PPARγ
expression (
p
= 0.008), but no influence on
LEP
or
ADIPOQ
expression was observed in Ad-36 (+) group. Those seropositive individuals also had higher expression of the miR-17 (
p
= 0.028) and lower levels of miR-155 (
p
= 0.031) in adipose tissue as compared to seronegative subjects.
Conclusions
Individuals with previous infection by Ad-36 had higher expression of the pro-adipogenic miR-17 and lower expression of the anti-adipogenic miR-155, which could lead to an increased adipogenic status by positively modulating
PPARγ
expression in adipose tissue from obese subjects.</description><subject>38/39</subject><subject>38/77</subject><subject>692/499</subject><subject>692/699/2743/393</subject><subject>82/1</subject><subject>Adenoviruses</subject><subject>Adipogenesis</subject><subject>Adipose tissue</subject><subject>Adipose tissues</subject><subject>Advertising executives</subject><subject>Animal models</subject><subject>Cell culture</subject><subject>Development and progression</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Epidemiology</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic research</subject><subject>Genetic transcription</subject><subject>Health Promotion and Disease Prevention</subject><subject>Internal Medicine</subject><subject>Maintenance</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Metabolic disorders</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Molecular modelling</subject><subject>Obesity</subject><subject>Post-transcription</subject><subject>Public Health</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Serology</subject><issn>0307-0565</issn><issn>1476-5497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kltr3DAQhUVpabZp_0AfiqFQ-uJ0dLcfl9BLILRQ2mdhy-NdLV7L1dgh-fdVsrk0pRQ9CEnfOcyMDmOvOZxwkNUHUlwaU4KAEsBoVdZP2Iora0qtavuUrUCCLUEbfcReEO0AQGsQz9mRFJUwtZUrhmdjPyw4eixiXzQdjvEipIUKaQrCFKdIYQ4XYb4q4ljMWyzwckpIFPLxRhGmuMEx-GIffIrfv66pCPmpRcKClnaHfqaX7FnfDISvbvdj9vPTxx-nX8rzb5_PTtfnpdeynkvllbZVZaTuUHOfC4euFaqrhai49rlgIWwFYGXfglBKKlmJVgKvEazqrTxm7w--U4q_FqTZ7QN5HIZmxLiQE0oaWZua84y-_QvdxSWNubpMWa5qYUT1QG2aAV0Y-zinxl-bunV24rwCDZk6-QeVV4d5KHHEPuT7R4J3fwi22AzzluKwzHmq9BgUBzCPlihh76YU9k26chzcdQjcIQQuh8DdhMDVWfTmtrWl3WN3L7n79QzIA0D5adxgeuj9P7a_AZq9uDI</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Manríquez, Víctor</creator><creator>Gutierrez, Alvaro</creator><creator>Morales, Alexis</creator><creator>Brito, Roberto</creator><creator>Pavez, Monica</creator><creator>Sapunar, Jorge</creator><creator>Fonseca, Luis</creator><creator>Molina, Víctor</creator><creator>Ortiz, Eugenia</creator><creator>Barra, Maria Ines</creator><creator>Reimer, Camila</creator><creator>Charles, Maria</creator><creator>Schneider, Constance</creator><creator>Cerda, Alvaro</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T2</scope><scope>7TK</scope><scope>7TS</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3428-8332</orcidid></search><sort><creationdate>20201101</creationdate><title>Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects</title><author>Manríquez, Víctor ; Gutierrez, Alvaro ; Morales, Alexis ; Brito, Roberto ; Pavez, Monica ; Sapunar, Jorge ; Fonseca, Luis ; Molina, Víctor ; Ortiz, Eugenia ; Barra, Maria Ines ; Reimer, Camila ; Charles, Maria ; Schneider, Constance ; Cerda, Alvaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-4c45788635de51c5650db24d922815c97322780073fb024434382b3019e074f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>38/39</topic><topic>38/77</topic><topic>692/499</topic><topic>692/699/2743/393</topic><topic>82/1</topic><topic>Adenoviruses</topic><topic>Adipogenesis</topic><topic>Adipose tissue</topic><topic>Adipose tissues</topic><topic>Advertising executives</topic><topic>Animal models</topic><topic>Cell culture</topic><topic>Development and progression</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Epidemiology</topic><topic>Epigenetic inheritance</topic><topic>Epigenetics</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic research</topic><topic>Genetic transcription</topic><topic>Health Promotion and Disease Prevention</topic><topic>Internal Medicine</topic><topic>Maintenance</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Metabolic disorders</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Molecular modelling</topic><topic>Obesity</topic><topic>Post-transcription</topic><topic>Public Health</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Serology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manríquez, Víctor</creatorcontrib><creatorcontrib>Gutierrez, Alvaro</creatorcontrib><creatorcontrib>Morales, Alexis</creatorcontrib><creatorcontrib>Brito, Roberto</creatorcontrib><creatorcontrib>Pavez, Monica</creatorcontrib><creatorcontrib>Sapunar, Jorge</creatorcontrib><creatorcontrib>Fonseca, Luis</creatorcontrib><creatorcontrib>Molina, Víctor</creatorcontrib><creatorcontrib>Ortiz, Eugenia</creatorcontrib><creatorcontrib>Barra, Maria Ines</creatorcontrib><creatorcontrib>Reimer, Camila</creatorcontrib><creatorcontrib>Charles, Maria</creatorcontrib><creatorcontrib>Schneider, Constance</creatorcontrib><creatorcontrib>Cerda, Alvaro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>International Journal of Obesity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manríquez, Víctor</au><au>Gutierrez, Alvaro</au><au>Morales, Alexis</au><au>Brito, Roberto</au><au>Pavez, Monica</au><au>Sapunar, Jorge</au><au>Fonseca, Luis</au><au>Molina, Víctor</au><au>Ortiz, Eugenia</au><au>Barra, Maria Ines</au><au>Reimer, Camila</au><au>Charles, Maria</au><au>Schneider, Constance</au><au>Cerda, Alvaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects</atitle><jtitle>International Journal of Obesity</jtitle><stitle>Int J Obes</stitle><addtitle>Int J Obes (Lond)</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>44</volume><issue>11</issue><spage>2303</spage><epage>2312</epage><pages>2303-2312</pages><issn>0307-0565</issn><eissn>1476-5497</eissn><abstract>Background
Infection by Adenovirus 36 (Ad-36) has been associated with adipogenesis using cell and animal models, and a high risk of developing obesity has been reported in Ad-36-seropositive individuals. However, molecular mechanisms involved in the maintenance over the years of adipogenesis associated with Ad-36 has not been investigated in human adipose tissue. Epigenetic mechanisms, such as micro-RNAs (miRNAs) that regulate gene expression at the post-transcriptional level, have shown an important role in the development and maintenance of metabolic diseases.
Aim
This study investigated the expression of miRNA associated with the adipogenic process in visceral adipose tissue from obese individuals according to Ad-36 serology.
Methods
Obese individuals were separated according to their status of Ad-36 serology in seropositive (Ad-36 (+);
n
= 29) and seronegative (Ad-36 (−);
n
= 28) groups. Additionally, a group of lean controls (
n
= 17) was selected to compare with obese individuals. Biopsies of visceral adipose tissue were obtained to evaluate miRNA and gene expression. The study of Ad-36 serology was carried out by ELISA. The expression of pro-adipogenic (miR-17 and miR-210) and anti-adipogenic (miR-155, miR-130 and miR-27a) miRNAs was evaluated using Taqman advanced miRNA assays by qPCR. The expression of adipogenes encoding
LEP
,
ADIPOQ
, and
PPARγ
was evaluated by Taqman predesigned assays through qPCR.
Results
The obese group had higher
LEP
(
p
< 0.001) and
PPARγ
(
p
= 0.016) expression and lower
ADIPOQ
expression (
p
= 0.017), and also had higher expression of miR-210 (
p
= 0.039), whereas lower expression of miR-155 (
p
= 0.019) and miR-27a (
p
= 0.028) as compared to lean controls. Higher
PPARγ
expression (
p
= 0.008), but no influence on
LEP
or
ADIPOQ
expression was observed in Ad-36 (+) group. Those seropositive individuals also had higher expression of the miR-17 (
p
= 0.028) and lower levels of miR-155 (
p
= 0.031) in adipose tissue as compared to seronegative subjects.
Conclusions
Individuals with previous infection by Ad-36 had higher expression of the pro-adipogenic miR-17 and lower expression of the anti-adipogenic miR-155, which could lead to an increased adipogenic status by positively modulating
PPARγ
expression in adipose tissue from obese subjects.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32826973</pmid><doi>10.1038/s41366-020-00654-9</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3428-8332</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0307-0565 |
| ispartof | International Journal of Obesity, 2020-11, Vol.44 (11), p.2303-2312 |
| issn | 0307-0565 1476-5497 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2436396911 |
| source | Nature; Alma/SFX Local Collection |
| subjects | 38/39 38/77 692/499 692/699/2743/393 82/1 Adenoviruses Adipogenesis Adipose tissue Adipose tissues Advertising executives Animal models Cell culture Development and progression Enzyme-linked immunosorbent assay Epidemiology Epigenetic inheritance Epigenetics Gene expression Genes Genetic aspects Genetic research Genetic transcription Health Promotion and Disease Prevention Internal Medicine Maintenance Medicine Medicine & Public Health Metabolic Diseases Metabolic disorders MicroRNA MicroRNAs miRNA Molecular modelling Obesity Post-transcription Public Health Ribonucleic acid RNA Serology |
| title | Influence of adenovirus 36 seropositivity on the expression of adipogenic microRNAs in obese subjects |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T10%3A43%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20adenovirus%2036%20seropositivity%20on%20the%20expression%20of%20adipogenic%20microRNAs%20in%20obese%20subjects&rft.jtitle=International%20Journal%20of%20Obesity&rft.au=Manr%C3%ADquez,%20V%C3%ADctor&rft.date=2020-11-01&rft.volume=44&rft.issue=11&rft.spage=2303&rft.epage=2312&rft.pages=2303-2312&rft.issn=0307-0565&rft.eissn=1476-5497&rft_id=info:doi/10.1038/s41366-020-00654-9&rft_dat=%3Cgale_proqu%3EA639118050%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2471492628&rft_id=info:pmid/32826973&rft_galeid=A639118050&rfr_iscdi=true |