Resistance to Boscalid in Botrytis cinerea From Greenhouse-Grown Tomato
Gray mold, caused by the fungus Pers ex Fr., is one of the most destructive spoilage diseases, severely affecting tomato production in Henan Province, China. Spraying fungicides from the flowering to the harvest stage is a necessary measure to reduce losses associated with infection. However, has de...
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| Veröffentlicht in: | Plant disease 2021-03, Vol.105 (3), p.628-635 |
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| creator | Liu, Shengming Fu, Liuyuan Tan, Huanhuan Jiang, Jia Che, Zhiping Tian, Yuee Chen, Genqiang |
| description | Gray mold, caused by the fungus
Pers ex Fr., is one of the most destructive spoilage diseases, severely affecting tomato production in Henan Province, China. Spraying fungicides from the flowering to the harvest stage is a necessary measure to reduce losses associated with
infection. However,
has developed resistance to fungicides in many countries. Boscalid is a succinate dehydrogenase inhibitor (SDHI) fungicide and was registered for the control of gray mold. In this study, a total of 269
isolates were collected from tomato in commercial greenhouses in different locations of Henan Province in 2014 and 2015. The sensitivity and resistance of
field isolates were determined based on mycelial growth. The effective concentration 50 ranged from 0.11 to 15.92 µg/ml and 0.16 to 8.54 µg/ml, in 2014 and 2015, respectively. The frequency of low resistance to boscalid was 12.6 and 7.6%, and moderate resistance was 2.7 and 1.3% in 2014 and 2015, respectively. No highly resistant isolates were found in Henan Province, China. Mycelial growth, mycelial dry weight, spore production, and pathogenicity were not significantly different between resistant and sensitive phenotypes of the
isolates. The results of cross-resistance testing showed no correlation between boscalid and carbendazim, procymidone, pyrimethanil, fluazinam, or fluopyram. In this study, the succinate dehydrogenase genes
(
),
(
), and
(
) were analyzed and compared in sensitive and low-resistance and moderately resistant
isolates to boscalid. Results showed that point mutations occurred simultaneously at
amino acid positions 85 (G85A), 93 (I93V), 158 (M158V), and 168 (V168I) in 4 out of 10 sensitive isolates and 23 of 26 low-resistance and 5 of 5 moderately resistant
isolates to boscalid. No point mutations were found in the
and
genes of all isolates. Furthermore, no point mutations were found in
,
, and
genes in 3 of 26 low-resistance
isolates to boscalid. Therefore, we speculate that the simultaneous point mutations in the
gene may not be related to the resistance of
to boscalid. These results suggested that there might be a substitution mechanism for the resistance of
to the SDHI fungicide boscalid. |
| doi_str_mv | 10.1094/PDIS-06-20-1191-RE |
| format | Article |
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Pers ex Fr., is one of the most destructive spoilage diseases, severely affecting tomato production in Henan Province, China. Spraying fungicides from the flowering to the harvest stage is a necessary measure to reduce losses associated with
infection. However,
has developed resistance to fungicides in many countries. Boscalid is a succinate dehydrogenase inhibitor (SDHI) fungicide and was registered for the control of gray mold. In this study, a total of 269
isolates were collected from tomato in commercial greenhouses in different locations of Henan Province in 2014 and 2015. The sensitivity and resistance of
field isolates were determined based on mycelial growth. The effective concentration 50 ranged from 0.11 to 15.92 µg/ml and 0.16 to 8.54 µg/ml, in 2014 and 2015, respectively. The frequency of low resistance to boscalid was 12.6 and 7.6%, and moderate resistance was 2.7 and 1.3% in 2014 and 2015, respectively. No highly resistant isolates were found in Henan Province, China. Mycelial growth, mycelial dry weight, spore production, and pathogenicity were not significantly different between resistant and sensitive phenotypes of the
isolates. The results of cross-resistance testing showed no correlation between boscalid and carbendazim, procymidone, pyrimethanil, fluazinam, or fluopyram. In this study, the succinate dehydrogenase genes
(
),
(
), and
(
) were analyzed and compared in sensitive and low-resistance and moderately resistant
isolates to boscalid. Results showed that point mutations occurred simultaneously at
amino acid positions 85 (G85A), 93 (I93V), 158 (M158V), and 168 (V168I) in 4 out of 10 sensitive isolates and 23 of 26 low-resistance and 5 of 5 moderately resistant
isolates to boscalid. No point mutations were found in the
and
genes of all isolates. Furthermore, no point mutations were found in
,
, and
genes in 3 of 26 low-resistance
isolates to boscalid. Therefore, we speculate that the simultaneous point mutations in the
gene may not be related to the resistance of
to boscalid. These results suggested that there might be a substitution mechanism for the resistance of
to the SDHI fungicide boscalid.</description><identifier>ISSN: 0191-2917</identifier><identifier>EISSN: 1943-7692</identifier><identifier>DOI: 10.1094/PDIS-06-20-1191-RE</identifier><identifier>PMID: 32820676</identifier><language>eng</language><publisher>United States</publisher><subject>Biphenyl Compounds ; Botrytis - genetics ; China ; Drug Resistance, Fungal - genetics ; Lycopersicon esculentum ; Niacinamide - analogs & derivatives ; Plant Diseases</subject><ispartof>Plant disease, 2021-03, Vol.105 (3), p.628-635</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-1f3b7293a4db6377b015c0d7ac7910cc296332b8633fe21e687488c9305a54293</citedby><cites>FETCH-LOGICAL-c303t-1f3b7293a4db6377b015c0d7ac7910cc296332b8633fe21e687488c9305a54293</cites><orcidid>0000-0002-4797-5994</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3710,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32820676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Shengming</creatorcontrib><creatorcontrib>Fu, Liuyuan</creatorcontrib><creatorcontrib>Tan, Huanhuan</creatorcontrib><creatorcontrib>Jiang, Jia</creatorcontrib><creatorcontrib>Che, Zhiping</creatorcontrib><creatorcontrib>Tian, Yuee</creatorcontrib><creatorcontrib>Chen, Genqiang</creatorcontrib><title>Resistance to Boscalid in Botrytis cinerea From Greenhouse-Grown Tomato</title><title>Plant disease</title><addtitle>Plant Dis</addtitle><description>Gray mold, caused by the fungus
Pers ex Fr., is one of the most destructive spoilage diseases, severely affecting tomato production in Henan Province, China. Spraying fungicides from the flowering to the harvest stage is a necessary measure to reduce losses associated with
infection. However,
has developed resistance to fungicides in many countries. Boscalid is a succinate dehydrogenase inhibitor (SDHI) fungicide and was registered for the control of gray mold. In this study, a total of 269
isolates were collected from tomato in commercial greenhouses in different locations of Henan Province in 2014 and 2015. The sensitivity and resistance of
field isolates were determined based on mycelial growth. The effective concentration 50 ranged from 0.11 to 15.92 µg/ml and 0.16 to 8.54 µg/ml, in 2014 and 2015, respectively. The frequency of low resistance to boscalid was 12.6 and 7.6%, and moderate resistance was 2.7 and 1.3% in 2014 and 2015, respectively. No highly resistant isolates were found in Henan Province, China. Mycelial growth, mycelial dry weight, spore production, and pathogenicity were not significantly different between resistant and sensitive phenotypes of the
isolates. The results of cross-resistance testing showed no correlation between boscalid and carbendazim, procymidone, pyrimethanil, fluazinam, or fluopyram. In this study, the succinate dehydrogenase genes
(
),
(
), and
(
) were analyzed and compared in sensitive and low-resistance and moderately resistant
isolates to boscalid. Results showed that point mutations occurred simultaneously at
amino acid positions 85 (G85A), 93 (I93V), 158 (M158V), and 168 (V168I) in 4 out of 10 sensitive isolates and 23 of 26 low-resistance and 5 of 5 moderately resistant
isolates to boscalid. No point mutations were found in the
and
genes of all isolates. Furthermore, no point mutations were found in
,
, and
genes in 3 of 26 low-resistance
isolates to boscalid. Therefore, we speculate that the simultaneous point mutations in the
gene may not be related to the resistance of
to boscalid. These results suggested that there might be a substitution mechanism for the resistance of
to the SDHI fungicide boscalid.</description><subject>Biphenyl Compounds</subject><subject>Botrytis - genetics</subject><subject>China</subject><subject>Drug Resistance, Fungal - genetics</subject><subject>Lycopersicon esculentum</subject><subject>Niacinamide - analogs & derivatives</subject><subject>Plant Diseases</subject><issn>0191-2917</issn><issn>1943-7692</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLAzEUhYMotlb_gAuZpZvozaPJZOmjHQsFpdZ1yGQyODIzqckU6b83pdXNfXHO4fIhdE3gjoDi92_Pi3cMAlPAhCiCV7MTNCaKMyyFoqdoDPsrVUSO0EWMXwDAucjP0YjRnIKQYoyKlYtNHExvXTb47NFHa9qmypo-zUPYDU3MbNO74Ew2D77LiuBc_-m30eEi-J8-W_vODP4SndWmje7q2CfoYz5bP73g5WuxeHpYYsuADZjUrJRUMcOrUjApSyBTC5U0VioC1lIlGKNlnmrtKHEilzzPrWIwNVOejBN0e8jdBP-9dXHQXROta1vTu_SUppwJpqiQPEnpQWqDjzG4Wm9C05mw0wT0HqDeA9QgNE17QqVXs2S6OeZvy85V_5Y_YuwXEt5qxw</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Liu, Shengming</creator><creator>Fu, Liuyuan</creator><creator>Tan, Huanhuan</creator><creator>Jiang, Jia</creator><creator>Che, Zhiping</creator><creator>Tian, Yuee</creator><creator>Chen, Genqiang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4797-5994</orcidid></search><sort><creationdate>202103</creationdate><title>Resistance to Boscalid in Botrytis cinerea From Greenhouse-Grown Tomato</title><author>Liu, Shengming ; Fu, Liuyuan ; Tan, Huanhuan ; Jiang, Jia ; Che, Zhiping ; Tian, Yuee ; Chen, Genqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-1f3b7293a4db6377b015c0d7ac7910cc296332b8633fe21e687488c9305a54293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biphenyl Compounds</topic><topic>Botrytis - genetics</topic><topic>China</topic><topic>Drug Resistance, Fungal - genetics</topic><topic>Lycopersicon esculentum</topic><topic>Niacinamide - analogs & derivatives</topic><topic>Plant Diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Shengming</creatorcontrib><creatorcontrib>Fu, Liuyuan</creatorcontrib><creatorcontrib>Tan, Huanhuan</creatorcontrib><creatorcontrib>Jiang, Jia</creatorcontrib><creatorcontrib>Che, Zhiping</creatorcontrib><creatorcontrib>Tian, Yuee</creatorcontrib><creatorcontrib>Chen, Genqiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Shengming</au><au>Fu, Liuyuan</au><au>Tan, Huanhuan</au><au>Jiang, Jia</au><au>Che, Zhiping</au><au>Tian, Yuee</au><au>Chen, Genqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance to Boscalid in Botrytis cinerea From Greenhouse-Grown Tomato</atitle><jtitle>Plant disease</jtitle><addtitle>Plant Dis</addtitle><date>2021-03</date><risdate>2021</risdate><volume>105</volume><issue>3</issue><spage>628</spage><epage>635</epage><pages>628-635</pages><issn>0191-2917</issn><eissn>1943-7692</eissn><abstract>Gray mold, caused by the fungus
Pers ex Fr., is one of the most destructive spoilage diseases, severely affecting tomato production in Henan Province, China. Spraying fungicides from the flowering to the harvest stage is a necessary measure to reduce losses associated with
infection. However,
has developed resistance to fungicides in many countries. Boscalid is a succinate dehydrogenase inhibitor (SDHI) fungicide and was registered for the control of gray mold. In this study, a total of 269
isolates were collected from tomato in commercial greenhouses in different locations of Henan Province in 2014 and 2015. The sensitivity and resistance of
field isolates were determined based on mycelial growth. The effective concentration 50 ranged from 0.11 to 15.92 µg/ml and 0.16 to 8.54 µg/ml, in 2014 and 2015, respectively. The frequency of low resistance to boscalid was 12.6 and 7.6%, and moderate resistance was 2.7 and 1.3% in 2014 and 2015, respectively. No highly resistant isolates were found in Henan Province, China. Mycelial growth, mycelial dry weight, spore production, and pathogenicity were not significantly different between resistant and sensitive phenotypes of the
isolates. The results of cross-resistance testing showed no correlation between boscalid and carbendazim, procymidone, pyrimethanil, fluazinam, or fluopyram. In this study, the succinate dehydrogenase genes
(
),
(
), and
(
) were analyzed and compared in sensitive and low-resistance and moderately resistant
isolates to boscalid. Results showed that point mutations occurred simultaneously at
amino acid positions 85 (G85A), 93 (I93V), 158 (M158V), and 168 (V168I) in 4 out of 10 sensitive isolates and 23 of 26 low-resistance and 5 of 5 moderately resistant
isolates to boscalid. No point mutations were found in the
and
genes of all isolates. Furthermore, no point mutations were found in
,
, and
genes in 3 of 26 low-resistance
isolates to boscalid. Therefore, we speculate that the simultaneous point mutations in the
gene may not be related to the resistance of
to boscalid. These results suggested that there might be a substitution mechanism for the resistance of
to the SDHI fungicide boscalid.</abstract><cop>United States</cop><pmid>32820676</pmid><doi>10.1094/PDIS-06-20-1191-RE</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4797-5994</orcidid></addata></record> |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | Biphenyl Compounds Botrytis - genetics China Drug Resistance, Fungal - genetics Lycopersicon esculentum Niacinamide - analogs & derivatives Plant Diseases |
| title | Resistance to Boscalid in Botrytis cinerea From Greenhouse-Grown Tomato |
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