Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells
Adipose-derived mesenchymal stem cells (Ad-MSCs) have been designated as the promising agents for clinical applications for easy accessibility, multi-linage differentiation and immunomodulation capacity. Despite this, optimal cell delivery conditions have remained as a clinical challenge and improve...
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| Veröffentlicht in: | Cell and tissue banking 2020-12, Vol.21 (4), p.643-653 |
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| creator | Heirani-Tabasi, Asieh Mirahmadi, Mahdi Mishan, Mohammad Amir Naderi-Meshkin, Hojjat Toosi, Shirin Matin, Maryam M. Bidkhori, Hamid Reza Bahrami, Ahmad Reza |
| description | Adipose-derived mesenchymal stem cells (Ad-MSCs) have been designated as the promising agents for clinical applications for easy accessibility, multi-linage differentiation and immunomodulation capacity. Despite this, optimal cell delivery conditions have remained as a clinical challenge and improvement of stem cell homing to the target organs is being considered as a major strategy in cell therapy systemic injection. It has been shown that homing of mesenchymal stem cells are increased when treated with physical or chemical hypoxia-mimicking factors, however, efficiency of different agents remained to be determined. In this study, hypoxia-mimicking agents, including valproic acid (VPA), cobalt chloride (CoCl
2
) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of
MAPK4
(associated with MAPK signaling pathway),
INPP4B
(associated with Inositol polyphosphate pathway),
VEGF-A
and
VEGF-C
(associated with cytokine–cytokine receptor pathways),
IL-8
and its receptor,
CXCR2
(associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl
2
only upregulated
VEGF-A
and
VEGF-C
gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways. |
| doi_str_mv | 10.1007/s10561-020-09851-2 |
| format | Article |
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2
) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of
MAPK4
(associated with MAPK signaling pathway),
INPP4B
(associated with Inositol polyphosphate pathway),
VEGF-A
and
VEGF-C
(associated with cytokine–cytokine receptor pathways),
IL-8
and its receptor,
CXCR2
(associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl
2
only upregulated
VEGF-A
and
VEGF-C
gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-020-09851-2</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; C gene ; Cell Biology ; Cell therapy ; Cobalt ; Cobalt chloride ; CXCR2 protein ; Cytokines ; Deferoxamine ; Gene expression ; Homing behavior ; Hypoxia ; Immunomodulation ; Inositol polyphosphate ; Interleukin 8 ; Life Sciences ; MAP kinase ; Mesenchymal stem cells ; Mimicry ; Signal transduction ; Stem cells ; Transplant Surgery ; Valproic acid ; Vascular endothelial growth factor ; Wound healing</subject><ispartof>Cell and tissue banking, 2020-12, Vol.21 (4), p.643-653</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-85e278a8e209f7371765fc50b493da24f910c0d727545eb00708bada19ab74723</citedby><cites>FETCH-LOGICAL-c352t-85e278a8e209f7371765fc50b493da24f910c0d727545eb00708bada19ab74723</cites><orcidid>0000-0002-0817-1950</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-020-09851-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-020-09851-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids></links><search><creatorcontrib>Heirani-Tabasi, Asieh</creatorcontrib><creatorcontrib>Mirahmadi, Mahdi</creatorcontrib><creatorcontrib>Mishan, Mohammad Amir</creatorcontrib><creatorcontrib>Naderi-Meshkin, Hojjat</creatorcontrib><creatorcontrib>Toosi, Shirin</creatorcontrib><creatorcontrib>Matin, Maryam M.</creatorcontrib><creatorcontrib>Bidkhori, Hamid Reza</creatorcontrib><creatorcontrib>Bahrami, Ahmad Reza</creatorcontrib><title>Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><description>Adipose-derived mesenchymal stem cells (Ad-MSCs) have been designated as the promising agents for clinical applications for easy accessibility, multi-linage differentiation and immunomodulation capacity. Despite this, optimal cell delivery conditions have remained as a clinical challenge and improvement of stem cell homing to the target organs is being considered as a major strategy in cell therapy systemic injection. It has been shown that homing of mesenchymal stem cells are increased when treated with physical or chemical hypoxia-mimicking factors, however, efficiency of different agents remained to be determined. In this study, hypoxia-mimicking agents, including valproic acid (VPA), cobalt chloride (CoCl
2
) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of
MAPK4
(associated with MAPK signaling pathway),
INPP4B
(associated with Inositol polyphosphate pathway),
VEGF-A
and
VEGF-C
(associated with cytokine–cytokine receptor pathways),
IL-8
and its receptor,
CXCR2
(associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl
2
only upregulated
VEGF-A
and
VEGF-C
gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>C gene</subject><subject>Cell Biology</subject><subject>Cell therapy</subject><subject>Cobalt</subject><subject>Cobalt chloride</subject><subject>CXCR2 protein</subject><subject>Cytokines</subject><subject>Deferoxamine</subject><subject>Gene expression</subject><subject>Homing behavior</subject><subject>Hypoxia</subject><subject>Immunomodulation</subject><subject>Inositol polyphosphate</subject><subject>Interleukin 8</subject><subject>Life Sciences</subject><subject>MAP kinase</subject><subject>Mesenchymal stem cells</subject><subject>Mimicry</subject><subject>Signal transduction</subject><subject>Stem cells</subject><subject>Transplant Surgery</subject><subject>Valproic acid</subject><subject>Vascular endothelial growth factor</subject><subject>Wound healing</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9P3DAQxaOKSqXQL9CTpV64GPwnXidHtGoBCYkLnK3Z7CRrmtipxyu6375OFwmJA6cZaX4zmvdeVX2X4lIKYa9ICrOSXCjBRdsYydWn6lQaq_mqkfVJ6XXT8lZr_aX6SvQsCmmVPq3yOk4zJE8xsLxDhn2PXSYWe7Y7zPGvBz75yXe_fRgYDBiWWWCTHxJkHwNPOELGLSM_BBgXaoa8e4EDMV84JAzd7jDByCjjxDocRzqvPvcwEn57rWfV06-fj-tbfv9wc7e-vuedNirzxqCyDTSoRNtbbaVdmb4zYlO3eguq7lspOrG1ypra4Kb4IJoNbEG2sLF1kXdWXRzvzin-2SNlN3laPoCAcU9O1drY4puoC_rjHfoc96koWigrW6mkEYVSR6pLkShh7-bkJ0gHJ4VbgnDHIFyx1_0Pwi1f6OMSFTgMmN5Of7D1D5IIjCk</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Heirani-Tabasi, Asieh</creator><creator>Mirahmadi, Mahdi</creator><creator>Mishan, Mohammad Amir</creator><creator>Naderi-Meshkin, Hojjat</creator><creator>Toosi, Shirin</creator><creator>Matin, Maryam M.</creator><creator>Bidkhori, Hamid Reza</creator><creator>Bahrami, Ahmad Reza</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0817-1950</orcidid></search><sort><creationdate>20201201</creationdate><title>Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells</title><author>Heirani-Tabasi, Asieh ; 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Despite this, optimal cell delivery conditions have remained as a clinical challenge and improvement of stem cell homing to the target organs is being considered as a major strategy in cell therapy systemic injection. It has been shown that homing of mesenchymal stem cells are increased when treated with physical or chemical hypoxia-mimicking factors, however, efficiency of different agents remained to be determined. In this study, hypoxia-mimicking agents, including valproic acid (VPA), cobalt chloride (CoCl
2
) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of
MAPK4
(associated with MAPK signaling pathway),
INPP4B
(associated with Inositol polyphosphate pathway),
VEGF-A
and
VEGF-C
(associated with cytokine–cytokine receptor pathways),
IL-8
and its receptor,
CXCR2
(associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl
2
only upregulated
VEGF-A
and
VEGF-C
gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10561-020-09851-2</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-0817-1950</orcidid></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biomedical and Life Sciences Biomedicine C gene Cell Biology Cell therapy Cobalt Cobalt chloride CXCR2 protein Cytokines Deferoxamine Gene expression Homing behavior Hypoxia Immunomodulation Inositol polyphosphate Interleukin 8 Life Sciences MAP kinase Mesenchymal stem cells Mimicry Signal transduction Stem cells Transplant Surgery Valproic acid Vascular endothelial growth factor Wound healing |
| title | Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells |
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