Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes
This study aimed to evaluate the relationship between antral follicular count (AFC) and ovarian volume (OV), preantral follicular population and survival, meiotic progression and ultrastructure of cumulus–oocyte complexes (COCs) after in vitro maturation. In experiment 1, the relationship between AF...
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| Veröffentlicht in: | Zygote (Cambridge) 2020-12, Vol.28 (6), p.495-503 |
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| creator | de Vasconcelos, Gisvani Lopes Maculan, Renata da Cunha, Ellen Vasconcelos Silva, Anderson Weiny Barbalho Batista, Ana Liza Souza Donato, Mariana Aragão Matos Peixoto, Christina Alves Silva, José Roberto Viana de Souza, José Camisão |
| description | This study aimed to evaluate the relationship between antral follicular count (AFC) and ovarian volume (OV), preantral follicular population and survival, meiotic progression and ultrastructure of cumulus–oocyte complexes (COCs) after
in vitro
maturation. In experiment 1, the relationship between AFC and preantral follicle population and survival was evaluated by classical histology. In experiment 2, the relationship among AFC, OV, ability of oocytes to resume meiosis and ultrastructure of
in vitro
matured bovine COCs was studied. A positive correlation (
P
< 0.05) between AFC and the numbers of healthy primordial, degenerate and total follicles was observed, as well as with healthy secondary follicles and total follicles. The numbers of grades I and II oocytes in ovaries of high AFC class were higher compared with those with intermediate or lower AFC. After
in vitro
maturation, COCs from ovaries of high AFC had a higher percentage of oocytes in metaphase II compared with those of intermediate and low AFC (
P
< 0.0001). Ovaries of intermediate AFC had a higher percentage of oocytes in metaphase II compared with ovaries with low AFC (
P
< 0.0001). The proportion of oocytes in metaphase I, telophase I and anaphase I in COCs from ovaries of intermediate AFC (26.04%) was higher (
P
< 0.05) compared with that seen in COCs of ovaries with high (8.55%) and low (14.15%) AFC. No differences in the ultrastructure of oocytes were seen. In conclusion, after
in vitro
maturation, cow ovaries with high AFC have higher numbers of oocytes that reach in metaphase II (MII), but they also have higher numbers of degenerated primordial and primary follicles. |
| doi_str_mv | 10.1017/S0967199420000386 |
| format | Article |
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in vitro
maturation. In experiment 1, the relationship between AFC and preantral follicle population and survival was evaluated by classical histology. In experiment 2, the relationship among AFC, OV, ability of oocytes to resume meiosis and ultrastructure of
in vitro
matured bovine COCs was studied. A positive correlation (
P
< 0.05) between AFC and the numbers of healthy primordial, degenerate and total follicles was observed, as well as with healthy secondary follicles and total follicles. The numbers of grades I and II oocytes in ovaries of high AFC class were higher compared with those with intermediate or lower AFC. After
in vitro
maturation, COCs from ovaries of high AFC had a higher percentage of oocytes in metaphase II compared with those of intermediate and low AFC (
P
< 0.0001). Ovaries of intermediate AFC had a higher percentage of oocytes in metaphase II compared with ovaries with low AFC (
P
< 0.0001). The proportion of oocytes in metaphase I, telophase I and anaphase I in COCs from ovaries of intermediate AFC (26.04%) was higher (
P
< 0.05) compared with that seen in COCs of ovaries with high (8.55%) and low (14.15%) AFC. No differences in the ultrastructure of oocytes were seen. In conclusion, after
in vitro
maturation, cow ovaries with high AFC have higher numbers of oocytes that reach in metaphase II (MII), but they also have higher numbers of degenerated primordial and primary follicles.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199420000386</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Anaphase ; Embryos ; Experiments ; Fertility ; Follicles ; Gametocytes ; Histology ; In vitro fertilization ; Maturation ; Meiosis ; Metaphase ; Oocytes ; Ovaries ; Population ; Reproductive technologies ; Survival ; Ultrastructure</subject><ispartof>Zygote (Cambridge), 2020-12, Vol.28 (6), p.495-503</ispartof><rights>The Author(s), 2020. Published by Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c306t-946c2d143b6197a600e84f5ffc2add115926446ad274760f147a1cb2bd81aa023</citedby><cites>FETCH-LOGICAL-c306t-946c2d143b6197a600e84f5ffc2add115926446ad274760f147a1cb2bd81aa023</cites><orcidid>0000-0002-5970-6177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>de Vasconcelos, Gisvani Lopes</creatorcontrib><creatorcontrib>Maculan, Renata</creatorcontrib><creatorcontrib>da Cunha, Ellen Vasconcelos</creatorcontrib><creatorcontrib>Silva, Anderson Weiny Barbalho</creatorcontrib><creatorcontrib>Batista, Ana Liza Souza</creatorcontrib><creatorcontrib>Donato, Mariana Aragão Matos</creatorcontrib><creatorcontrib>Peixoto, Christina Alves</creatorcontrib><creatorcontrib>Silva, José Roberto Viana</creatorcontrib><creatorcontrib>de Souza, José Camisão</creatorcontrib><title>Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes</title><title>Zygote (Cambridge)</title><description>This study aimed to evaluate the relationship between antral follicular count (AFC) and ovarian volume (OV), preantral follicular population and survival, meiotic progression and ultrastructure of cumulus–oocyte complexes (COCs) after
in vitro
maturation. In experiment 1, the relationship between AFC and preantral follicle population and survival was evaluated by classical histology. In experiment 2, the relationship among AFC, OV, ability of oocytes to resume meiosis and ultrastructure of
in vitro
matured bovine COCs was studied. A positive correlation (
P
< 0.05) between AFC and the numbers of healthy primordial, degenerate and total follicles was observed, as well as with healthy secondary follicles and total follicles. The numbers of grades I and II oocytes in ovaries of high AFC class were higher compared with those with intermediate or lower AFC. After
in vitro
maturation, COCs from ovaries of high AFC had a higher percentage of oocytes in metaphase II compared with those of intermediate and low AFC (
P
< 0.0001). Ovaries of intermediate AFC had a higher percentage of oocytes in metaphase II compared with ovaries with low AFC (
P
< 0.0001). The proportion of oocytes in metaphase I, telophase I and anaphase I in COCs from ovaries of intermediate AFC (26.04%) was higher (
P
< 0.05) compared with that seen in COCs of ovaries with high (8.55%) and low (14.15%) AFC. No differences in the ultrastructure of oocytes were seen. In conclusion, after
in vitro
maturation, cow ovaries with high AFC have higher numbers of oocytes that reach in metaphase II (MII), but they also have higher numbers of degenerated primordial and primary follicles.</description><subject>Anaphase</subject><subject>Embryos</subject><subject>Experiments</subject><subject>Fertility</subject><subject>Follicles</subject><subject>Gametocytes</subject><subject>Histology</subject><subject>In vitro fertilization</subject><subject>Maturation</subject><subject>Meiosis</subject><subject>Metaphase</subject><subject>Oocytes</subject><subject>Ovaries</subject><subject>Population</subject><subject>Reproductive technologies</subject><subject>Survival</subject><subject>Ultrastructure</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkctu1TAQhi0EEofCA7AbiQ2LBnyLnSyrikulSiyAdTTHcagrJw6-hHbHO_BkvAJPUh9OkRB4Y8n_N9-MNYQ8Z_QVo0y__kh7pVnfS07rEZ16QHZMqr7ptKAPye4QN4f8MXmS0nVltO7ljvw8W3JED1Pw3pniMYIJZcmAywguJ4jWY3ZhSVduhW8uX0HYMDpcYAu-zPYU1mjxb4m3sIa1HMt-e1KJm9vQn0II5jZbmK0L2ZlaGr5Em9IfsPjqSTkWk0u0ECZwtY_LMcCMh6cR9mFziwVT5uJL-vX9x73ShHn19samp-TRhD7ZZ_f3Cfn89s2n8_fN5Yd3F-dnl40RVOWml8rwkUmxV6zXqCi1nZzaaTIcx5GxtudKSoUj11IrOjGpkZk9348dQ6RcnJCXR2_9xNdiUx5ml4z1HhcbShq4FG0rOGeqoi_-Qa9DiUudbuCtFKoTHaOVYkfKxJBStNOwRjdjvB0YHQ5LHv5bsrgDQ4uhNg</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>de Vasconcelos, Gisvani Lopes</creator><creator>Maculan, Renata</creator><creator>da Cunha, Ellen Vasconcelos</creator><creator>Silva, Anderson Weiny Barbalho</creator><creator>Batista, Ana Liza Souza</creator><creator>Donato, Mariana Aragão Matos</creator><creator>Peixoto, Christina Alves</creator><creator>Silva, José Roberto Viana</creator><creator>de Souza, José Camisão</creator><general>Cambridge University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5970-6177</orcidid></search><sort><creationdate>20201201</creationdate><title>Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes</title><author>de Vasconcelos, Gisvani Lopes ; Maculan, Renata ; da Cunha, Ellen Vasconcelos ; Silva, Anderson Weiny Barbalho ; Batista, Ana Liza Souza ; Donato, Mariana Aragão Matos ; Peixoto, Christina Alves ; Silva, José Roberto Viana ; de Souza, José Camisão</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-946c2d143b6197a600e84f5ffc2add115926446ad274760f147a1cb2bd81aa023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Anaphase</topic><topic>Embryos</topic><topic>Experiments</topic><topic>Fertility</topic><topic>Follicles</topic><topic>Gametocytes</topic><topic>Histology</topic><topic>In vitro fertilization</topic><topic>Maturation</topic><topic>Meiosis</topic><topic>Metaphase</topic><topic>Oocytes</topic><topic>Ovaries</topic><topic>Population</topic><topic>Reproductive technologies</topic><topic>Survival</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Vasconcelos, Gisvani Lopes</creatorcontrib><creatorcontrib>Maculan, Renata</creatorcontrib><creatorcontrib>da Cunha, Ellen Vasconcelos</creatorcontrib><creatorcontrib>Silva, Anderson Weiny Barbalho</creatorcontrib><creatorcontrib>Batista, Ana Liza Souza</creatorcontrib><creatorcontrib>Donato, Mariana Aragão Matos</creatorcontrib><creatorcontrib>Peixoto, Christina Alves</creatorcontrib><creatorcontrib>Silva, José Roberto Viana</creatorcontrib><creatorcontrib>de Souza, José Camisão</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Vasconcelos, Gisvani Lopes</au><au>Maculan, Renata</au><au>da Cunha, Ellen Vasconcelos</au><au>Silva, Anderson Weiny Barbalho</au><au>Batista, Ana Liza Souza</au><au>Donato, Mariana Aragão Matos</au><au>Peixoto, Christina Alves</au><au>Silva, José Roberto Viana</au><au>de Souza, José Camisão</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes</atitle><jtitle>Zygote (Cambridge)</jtitle><date>2020-12-01</date><risdate>2020</risdate><volume>28</volume><issue>6</issue><spage>495</spage><epage>503</epage><pages>495-503</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>This study aimed to evaluate the relationship between antral follicular count (AFC) and ovarian volume (OV), preantral follicular population and survival, meiotic progression and ultrastructure of cumulus–oocyte complexes (COCs) after
in vitro
maturation. In experiment 1, the relationship between AFC and preantral follicle population and survival was evaluated by classical histology. In experiment 2, the relationship among AFC, OV, ability of oocytes to resume meiosis and ultrastructure of
in vitro
matured bovine COCs was studied. A positive correlation (
P
< 0.05) between AFC and the numbers of healthy primordial, degenerate and total follicles was observed, as well as with healthy secondary follicles and total follicles. The numbers of grades I and II oocytes in ovaries of high AFC class were higher compared with those with intermediate or lower AFC. After
in vitro
maturation, COCs from ovaries of high AFC had a higher percentage of oocytes in metaphase II compared with those of intermediate and low AFC (
P
< 0.0001). Ovaries of intermediate AFC had a higher percentage of oocytes in metaphase II compared with ovaries with low AFC (
P
< 0.0001). The proportion of oocytes in metaphase I, telophase I and anaphase I in COCs from ovaries of intermediate AFC (26.04%) was higher (
P
< 0.05) compared with that seen in COCs of ovaries with high (8.55%) and low (14.15%) AFC. No differences in the ultrastructure of oocytes were seen. In conclusion, after
in vitro
maturation, cow ovaries with high AFC have higher numbers of oocytes that reach in metaphase II (MII), but they also have higher numbers of degenerated primordial and primary follicles.</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><doi>10.1017/S0967199420000386</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5970-6177</orcidid></addata></record> |
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| ispartof | Zygote (Cambridge), 2020-12, Vol.28 (6), p.495-503 |
| issn | 0967-1994 1469-8730 |
| language | eng |
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| source | Cambridge University Press Journals Complete |
| subjects | Anaphase Embryos Experiments Fertility Follicles Gametocytes Histology In vitro fertilization Maturation Meiosis Metaphase Oocytes Ovaries Population Reproductive technologies Survival Ultrastructure |
| title | Antral follicular count and its relationship with ovarian volume, preantral follicle population and survival, oocyte meiotic progression and ultrastructure of in vitro matured bovine cumulus–oocyte complexes |
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