Penaeus stylirostris densovirus proteins CP and NS1 interact with peritrophin of Litopenaeus vannamei
The Penaeus stylirostris densovirus (PstDNV) is a major virus of shrimps that severely harms the shrimp farming industry. Peritrophin is a peritrophic membrane protein with chitin binding activity. To examine the roles of peritrophin in viral infection, we used yeast two-hybrid to analyze the intera...
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| Veröffentlicht in: | Fish & shellfish immunology 2020-11, Vol.106, p.357-364 |
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| container_title | Fish & shellfish immunology |
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| creator | Yang, Chunling Liu, Qingyun Peng, Min Chen, Xiuli Zhu, Weilin Chen, Xiaohan Li, Qiangyong Zeng, Digang Zhao, Yongzhen |
| description | The Penaeus stylirostris densovirus (PstDNV) is a major virus of shrimps that severely harms the shrimp farming industry. Peritrophin is a peritrophic membrane protein with chitin binding activity. To examine the roles of peritrophin in viral infection, we used yeast two-hybrid to analyze the interaction between the Pacific white shrimp (Litopenaeus vannamei) peritrophin and PstDNV proteins (CP, NS1 and NS2). The yeast two-hybrid results showed that NS1 and peritrophin had an interaction, CP and peritrophin had an interaction as well, and NS2 had no interaction with peritrophin. We validated the interactions with GST pull-down assays. We then conducted RNA interference and qRT-PCR. The results showed that when pre-injection of dsRNA-peritrophin, the quantity of PstDNV in the shrimps injected with viruses was significantly lower than in the control group (P |
| doi_str_mv | 10.1016/j.fsi.2020.08.006 |
| format | Article |
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•The PstDNV protein NS1 interacts with peritrophin of L. vannamei.•The PstDNV protein CP also interacts with peritrophin of L. vannamei.•When L. vannamei peritrophin gene expression was silenced, PsDNV proliferation in L. vannamei was reduced.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2020.08.006</identifier><identifier>PMID: 32791095</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Arthropod Proteins - genetics ; Arthropod Proteins - immunology ; Capsid Proteins - physiology ; Densovirinae - physiology ; Interaction ; Litopenaeus vannamei ; Penaeidae - genetics ; Penaeidae - immunology ; Penaeus stylirostris densovirus ; Proteins ; Real-Time Polymerase Chain Reaction ; RNA Interference ; Viral Nonstructural Proteins - physiology</subject><ispartof>Fish & shellfish immunology, 2020-11, Vol.106, p.357-364</ispartof><rights>2020</rights><rights>Copyright © 2020. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-69006105983ee8e22f3e5a98b7248795d35f4d6be9f6fd253051efd431a949fa3</citedby><cites>FETCH-LOGICAL-c353t-69006105983ee8e22f3e5a98b7248795d35f4d6be9f6fd253051efd431a949fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2020.08.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32791095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Chunling</creatorcontrib><creatorcontrib>Liu, Qingyun</creatorcontrib><creatorcontrib>Peng, Min</creatorcontrib><creatorcontrib>Chen, Xiuli</creatorcontrib><creatorcontrib>Zhu, Weilin</creatorcontrib><creatorcontrib>Chen, Xiaohan</creatorcontrib><creatorcontrib>Li, Qiangyong</creatorcontrib><creatorcontrib>Zeng, Digang</creatorcontrib><creatorcontrib>Zhao, Yongzhen</creatorcontrib><title>Penaeus stylirostris densovirus proteins CP and NS1 interact with peritrophin of Litopenaeus vannamei</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>The Penaeus stylirostris densovirus (PstDNV) is a major virus of shrimps that severely harms the shrimp farming industry. Peritrophin is a peritrophic membrane protein with chitin binding activity. To examine the roles of peritrophin in viral infection, we used yeast two-hybrid to analyze the interaction between the Pacific white shrimp (Litopenaeus vannamei) peritrophin and PstDNV proteins (CP, NS1 and NS2). The yeast two-hybrid results showed that NS1 and peritrophin had an interaction, CP and peritrophin had an interaction as well, and NS2 had no interaction with peritrophin. We validated the interactions with GST pull-down assays. We then conducted RNA interference and qRT-PCR. The results showed that when pre-injection of dsRNA-peritrophin, the quantity of PstDNV in the shrimps injected with viruses was significantly lower than in the control group (P < 0.01), indicating the viral infection was decreased when the peritrophin gene expression was inhibited. The results indicated that peritrophin of L. vannamei participated in the PstDNV infection.
•The PstDNV protein NS1 interacts with peritrophin of L. vannamei.•The PstDNV protein CP also interacts with peritrophin of L. vannamei.•When L. vannamei peritrophin gene expression was silenced, PsDNV proliferation in L. vannamei was reduced.</description><subject>Animals</subject><subject>Arthropod Proteins - genetics</subject><subject>Arthropod Proteins - immunology</subject><subject>Capsid Proteins - physiology</subject><subject>Densovirinae - physiology</subject><subject>Interaction</subject><subject>Litopenaeus vannamei</subject><subject>Penaeidae - genetics</subject><subject>Penaeidae - immunology</subject><subject>Penaeus stylirostris densovirus</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA Interference</subject><subject>Viral Nonstructural Proteins - physiology</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rGzEQhkVISVI3PyCXomMuu5VW0u6KnIpJP8CkgaRnIa9GZIyt3UqyQ_59tdjpsacRw6OXdx5CbjirOePtl03tE9YNa1jN-pqx9oxccaZVpbXszue3YpVsZX9JPqa0YYUQLbsgl6Lp9AxeEXiEYGGfaMpvW4xjyhETdRDSeMBY9lMcM2BIdPlIbXD04YlTDBmiHTJ9xfxCJ4iY4zi9YKCjpyvM43QKPdgQ7A7wE_ng7TbB9WkuyO9v98_LH9Xq1_efy6-rahBK5KrVpWEprXsB0EPTeAHK6n7dNbLvtHJCeenaNWjfetcowRQH76TgVkvtrViQ22Nuaf1nDymbHaYBtlsbYNwn00ghZSe6XhWUH9GhHJ0ieDNF3Nn4Zjgzs12zMcWume0a1pvZ3YJ8PsXv1ztw_3686yzA3RGAcuQBIZo0IIQBHEYYsnEj_if-L0q-i5g</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Yang, Chunling</creator><creator>Liu, Qingyun</creator><creator>Peng, Min</creator><creator>Chen, Xiuli</creator><creator>Zhu, Weilin</creator><creator>Chen, Xiaohan</creator><creator>Li, Qiangyong</creator><creator>Zeng, Digang</creator><creator>Zhao, Yongzhen</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202011</creationdate><title>Penaeus stylirostris densovirus proteins CP and NS1 interact with peritrophin of Litopenaeus vannamei</title><author>Yang, Chunling ; Liu, Qingyun ; Peng, Min ; Chen, Xiuli ; Zhu, Weilin ; Chen, Xiaohan ; Li, Qiangyong ; Zeng, Digang ; Zhao, Yongzhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-69006105983ee8e22f3e5a98b7248795d35f4d6be9f6fd253051efd431a949fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Arthropod Proteins - genetics</topic><topic>Arthropod Proteins - immunology</topic><topic>Capsid Proteins - physiology</topic><topic>Densovirinae - physiology</topic><topic>Interaction</topic><topic>Litopenaeus vannamei</topic><topic>Penaeidae - genetics</topic><topic>Penaeidae - immunology</topic><topic>Penaeus stylirostris densovirus</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA Interference</topic><topic>Viral Nonstructural Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Chunling</creatorcontrib><creatorcontrib>Liu, Qingyun</creatorcontrib><creatorcontrib>Peng, Min</creatorcontrib><creatorcontrib>Chen, Xiuli</creatorcontrib><creatorcontrib>Zhu, Weilin</creatorcontrib><creatorcontrib>Chen, Xiaohan</creatorcontrib><creatorcontrib>Li, Qiangyong</creatorcontrib><creatorcontrib>Zeng, Digang</creatorcontrib><creatorcontrib>Zhao, Yongzhen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Chunling</au><au>Liu, Qingyun</au><au>Peng, Min</au><au>Chen, Xiuli</au><au>Zhu, Weilin</au><au>Chen, Xiaohan</au><au>Li, Qiangyong</au><au>Zeng, Digang</au><au>Zhao, Yongzhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Penaeus stylirostris densovirus proteins CP and NS1 interact with peritrophin of Litopenaeus vannamei</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2020-11</date><risdate>2020</risdate><volume>106</volume><spage>357</spage><epage>364</epage><pages>357-364</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>The Penaeus stylirostris densovirus (PstDNV) is a major virus of shrimps that severely harms the shrimp farming industry. Peritrophin is a peritrophic membrane protein with chitin binding activity. To examine the roles of peritrophin in viral infection, we used yeast two-hybrid to analyze the interaction between the Pacific white shrimp (Litopenaeus vannamei) peritrophin and PstDNV proteins (CP, NS1 and NS2). The yeast two-hybrid results showed that NS1 and peritrophin had an interaction, CP and peritrophin had an interaction as well, and NS2 had no interaction with peritrophin. We validated the interactions with GST pull-down assays. We then conducted RNA interference and qRT-PCR. The results showed that when pre-injection of dsRNA-peritrophin, the quantity of PstDNV in the shrimps injected with viruses was significantly lower than in the control group (P < 0.01), indicating the viral infection was decreased when the peritrophin gene expression was inhibited. The results indicated that peritrophin of L. vannamei participated in the PstDNV infection.
•The PstDNV protein NS1 interacts with peritrophin of L. vannamei.•The PstDNV protein CP also interacts with peritrophin of L. vannamei.•When L. vannamei peritrophin gene expression was silenced, PsDNV proliferation in L. vannamei was reduced.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32791095</pmid><doi>10.1016/j.fsi.2020.08.006</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Arthropod Proteins - genetics Arthropod Proteins - immunology Capsid Proteins - physiology Densovirinae - physiology Interaction Litopenaeus vannamei Penaeidae - genetics Penaeidae - immunology Penaeus stylirostris densovirus Proteins Real-Time Polymerase Chain Reaction RNA Interference Viral Nonstructural Proteins - physiology |
| title | Penaeus stylirostris densovirus proteins CP and NS1 interact with peritrophin of Litopenaeus vannamei |
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