One-Pot Synthesis of Adipic Acid from Guaiacol in Escherichia coli
Adipic acid is one of the most important small molecules in the modern chemical industry. However, the damaging environmental impact of the current industrial synthesis of adipic acid has necessitated the development of greener, biobased approaches to its manufacture. Herein we report the first one-...
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| Veröffentlicht in: | ACS synthetic biology 2020-09, Vol.9 (9), p.2472-2476 |
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| container_title | ACS synthetic biology |
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| creator | Suitor, Jack T Varzandeh, Simon Wallace, Stephen |
| description | Adipic acid is one of the most important small molecules in the modern chemical industry. However, the damaging environmental impact of the current industrial synthesis of adipic acid has necessitated the development of greener, biobased approaches to its manufacture. Herein we report the first one-pot synthesis of adipic acid from guaiacol, a lignin-derived feedstock, using genetically engineered whole-cells of Escherichia coli. The reaction is mild, efficient, requires no additional additives or reagents, and produces no byproducts. This study demonstrates how modern synthetic biology can be used to valorize abundant feedstocks into industrially relevant small molecules in living cells. |
| doi_str_mv | 10.1021/acssynbio.0c00254 |
| format | Article |
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This study demonstrates how modern synthetic biology can be used to valorize abundant feedstocks into industrially relevant small molecules in living cells.</description><subject>Adipates - metabolism</subject><subject>Bacillus coagulans - enzymology</subject><subject>Dioxygenases - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Guaiacol - metabolism</subject><subject>Metabolic Engineering - methods</subject><subject>Oxidoreductases - genetics</subject><subject>Plasmids - genetics</subject><subject>Plasmids - metabolism</subject><subject>Pseudomonas putida - enzymology</subject><issn>2161-5063</issn><issn>2161-5063</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LwzAYh4Mobsx9AC-So5fO_G96nGNOYTBBPYc0TVhG28ykPezb27E5PPle8hKe3w_eB4B7jGYYEfykTUqHtvRhhgxChLMrMCZY4IwjQa__7CMwTWmHhuGccipvwYiSXIqC0DF43rQ2ew8d_Di03dYmn2BwcF75vTdwbnwFXQwNXPXaaxNq6Fu4TGZrozdbr-Hw5e_AjdN1stPzOwFfL8vPxWu23qzeFvN1pqkkXcZlIQojcyqNLqksWKVxgbg1Dhe5ZUXuODYltk5QQZmzFWdWWkOdrDhloqIT8Hjq3cfw3dvUqcYnY-tatzb0SRFGGRKY5HhA8Qk1MaQUrVP76BsdDwojdbSnLvbU2d6QeTjX92Vjq0vi19UAZCdgyKpd6GM7XPtP4Q-UzXsJ</recordid><startdate>20200918</startdate><enddate>20200918</enddate><creator>Suitor, Jack T</creator><creator>Varzandeh, Simon</creator><creator>Wallace, Stephen</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200918</creationdate><title>One-Pot Synthesis of Adipic Acid from Guaiacol in Escherichia coli</title><author>Suitor, Jack T ; Varzandeh, Simon ; Wallace, Stephen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a382t-58969c8738cab3894da1905ecf197e497f51cb1ef63634fed54e8ec3f8d5346d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipates - metabolism</topic><topic>Bacillus coagulans - enzymology</topic><topic>Dioxygenases - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Guaiacol - metabolism</topic><topic>Metabolic Engineering - methods</topic><topic>Oxidoreductases - genetics</topic><topic>Plasmids - genetics</topic><topic>Plasmids - metabolism</topic><topic>Pseudomonas putida - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suitor, Jack T</creatorcontrib><creatorcontrib>Varzandeh, Simon</creatorcontrib><creatorcontrib>Wallace, Stephen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS synthetic biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suitor, Jack T</au><au>Varzandeh, Simon</au><au>Wallace, Stephen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>One-Pot Synthesis of Adipic Acid from Guaiacol in Escherichia coli</atitle><jtitle>ACS synthetic biology</jtitle><addtitle>ACS Synth. Biol</addtitle><date>2020-09-18</date><risdate>2020</risdate><volume>9</volume><issue>9</issue><spage>2472</spage><epage>2476</epage><pages>2472-2476</pages><issn>2161-5063</issn><eissn>2161-5063</eissn><abstract>Adipic acid is one of the most important small molecules in the modern chemical industry. However, the damaging environmental impact of the current industrial synthesis of adipic acid has necessitated the development of greener, biobased approaches to its manufacture. Herein we report the first one-pot synthesis of adipic acid from guaiacol, a lignin-derived feedstock, using genetically engineered whole-cells of Escherichia coli. The reaction is mild, efficient, requires no additional additives or reagents, and produces no byproducts. 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| subjects | Adipates - metabolism Bacillus coagulans - enzymology Dioxygenases - genetics Escherichia coli - genetics Escherichia coli - metabolism Guaiacol - metabolism Metabolic Engineering - methods Oxidoreductases - genetics Plasmids - genetics Plasmids - metabolism Pseudomonas putida - enzymology |
| title | One-Pot Synthesis of Adipic Acid from Guaiacol in Escherichia coli |
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