The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease
Chronic kidney disease (CKD) impairs osteoblast/osteoclast balance and damages bone structure with diminished mineralization and results in bone restoration disorders. In this study, we investigate the effects of adipose-derived stromal vascular fraction and platelet-rich plasma (PRP) on bone healin...
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| Veröffentlicht in: | Annals of plastic surgery 2020-09, Vol.85 (3), p.316-323 |
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| creator | Eyuboglu, Atilla Adnan Arpaci, Enver Albayati, Abbas Uysal, Ahmet Cagri Terzi, Aysen Bozalioglu, Sema Turnaoglu, Hale Balcik, Cenk Ozkan, Burak Ertas, Nilgun Markal |
| description | Chronic kidney disease (CKD) impairs osteoblast/osteoclast balance and damages bone structure with diminished mineralization and results in bone restoration disorders. In this study, we investigate the effects of adipose-derived stromal vascular fraction and platelet-rich plasma (PRP) on bone healing model in rats with CKD.
Sprague-Dawley rats were separated into 4 groups. All groups except group I (healthy control) had CKD surgery using 5/6 nephrectomy model. All groups had intramedullary pin fixation after receiving bone fracture using drilling tools. Group II rats were used as control group for CKD. Group III rats received PRP treatment on fracture site. Group IV rats received PRP and stromal vascular fraction treatment on fracture site.Weight loss and blood samples were followed at the time of kidney surgery, third, sixth, and 12th weeks. Bone healing and callus formations were compared, biomechanically, radiologically, histopathologically, and immunohistochemically. Osteoblastic transformation of stem cells was assessed with DiI staining.
Negative effects of CKD on bone healing were reduced by increasing mechanical, histological, radiological, and biochemical properties of the bone with stromal vascular fraction and PRP treatments. Although thickness of callus tissue delayed bone healing process, it also enhanced biomechanical features and bone tissue organization.
Platelet-rich plasma and adipose-derived stromal vascular fraction treatments were effective for bone healing in animal model, which can be promising for clinical trials. |
| doi_str_mv | 10.1097/SAP.0000000000002396 |
| format | Article |
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Sprague-Dawley rats were separated into 4 groups. All groups except group I (healthy control) had CKD surgery using 5/6 nephrectomy model. All groups had intramedullary pin fixation after receiving bone fracture using drilling tools. Group II rats were used as control group for CKD. Group III rats received PRP treatment on fracture site. Group IV rats received PRP and stromal vascular fraction treatment on fracture site.Weight loss and blood samples were followed at the time of kidney surgery, third, sixth, and 12th weeks. Bone healing and callus formations were compared, biomechanically, radiologically, histopathologically, and immunohistochemically. Osteoblastic transformation of stem cells was assessed with DiI staining.
Negative effects of CKD on bone healing were reduced by increasing mechanical, histological, radiological, and biochemical properties of the bone with stromal vascular fraction and PRP treatments. Although thickness of callus tissue delayed bone healing process, it also enhanced biomechanical features and bone tissue organization.
Platelet-rich plasma and adipose-derived stromal vascular fraction treatments were effective for bone healing in animal model, which can be promising for clinical trials.</description><identifier>ISSN: 0148-7043</identifier><identifier>EISSN: 1536-3708</identifier><identifier>DOI: 10.1097/SAP.0000000000002396</identifier><identifier>PMID: 32784349</identifier><language>eng</language><publisher>United States: Lippincott Williams & Wilkins</publisher><subject>Adipose Tissue ; Animals ; Platelet-Rich Plasma ; Rats ; Rats, Sprague-Dawley ; Renal Insufficiency, Chronic - therapy ; Wound Healing</subject><ispartof>Annals of plastic surgery, 2020-09, Vol.85 (3), p.316-323</ispartof><rights>Lippincott Williams & Wilkins</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3523-fe9f75a128f95f4248d0196c46f704154d859b26259c231462f323a0431d23363</citedby><cites>FETCH-LOGICAL-c3523-fe9f75a128f95f4248d0196c46f704154d859b26259c231462f323a0431d23363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32784349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eyuboglu, Atilla Adnan</creatorcontrib><creatorcontrib>Arpaci, Enver</creatorcontrib><creatorcontrib>Albayati, Abbas</creatorcontrib><creatorcontrib>Uysal, Ahmet Cagri</creatorcontrib><creatorcontrib>Terzi, Aysen</creatorcontrib><creatorcontrib>Bozalioglu, Sema</creatorcontrib><creatorcontrib>Turnaoglu, Hale</creatorcontrib><creatorcontrib>Balcik, Cenk</creatorcontrib><creatorcontrib>Ozkan, Burak</creatorcontrib><creatorcontrib>Ertas, Nilgun Markal</creatorcontrib><title>The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease</title><title>Annals of plastic surgery</title><addtitle>Ann Plast Surg</addtitle><description>Chronic kidney disease (CKD) impairs osteoblast/osteoclast balance and damages bone structure with diminished mineralization and results in bone restoration disorders. In this study, we investigate the effects of adipose-derived stromal vascular fraction and platelet-rich plasma (PRP) on bone healing model in rats with CKD.
Sprague-Dawley rats were separated into 4 groups. All groups except group I (healthy control) had CKD surgery using 5/6 nephrectomy model. All groups had intramedullary pin fixation after receiving bone fracture using drilling tools. Group II rats were used as control group for CKD. Group III rats received PRP treatment on fracture site. Group IV rats received PRP and stromal vascular fraction treatment on fracture site.Weight loss and blood samples were followed at the time of kidney surgery, third, sixth, and 12th weeks. Bone healing and callus formations were compared, biomechanically, radiologically, histopathologically, and immunohistochemically. Osteoblastic transformation of stem cells was assessed with DiI staining.
Negative effects of CKD on bone healing were reduced by increasing mechanical, histological, radiological, and biochemical properties of the bone with stromal vascular fraction and PRP treatments. Although thickness of callus tissue delayed bone healing process, it also enhanced biomechanical features and bone tissue organization.
Platelet-rich plasma and adipose-derived stromal vascular fraction treatments were effective for bone healing in animal model, which can be promising for clinical trials.</description><subject>Adipose Tissue</subject><subject>Animals</subject><subject>Platelet-Rich Plasma</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Renal Insufficiency, Chronic - therapy</subject><subject>Wound Healing</subject><issn>0148-7043</issn><issn>1536-3708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkd9OFTEQxhsjkSP6Bsb00puF_tvu9vJ4ADFgJIB6uSndqVvtbg9tV8I7-ND0cFCMczOZzMxvMt-H0BtK9ilRzcHl8nyf_BOMK_kMLWjNZcUb0j5HC0JFWzVE8F30MqUfhFDWCvkC7XLWtIILtUC_rwbAR9aCyQkHi5e9W4cE-BCi-wU9vswxjNrjrzqZ2euIj6M22YUJ66nH515n8JCrC2eGTZVGjUvvfZgAn4D2bvq-oWp8oTP-FHrw-JvLA14NMUzO4FPXT3CHD10CneAV2rHaJ3j9mPfQl-Ojq9VJdfb5w8fV8qwyvGa8sqBsU-vyjFW1FUy0PaFKGiFteZbWom9rdc0kq5VhnArJLGdcFx1ozziXfA-923LXMdzMkHI3umTAez1BmFPHijakbpUQZVRsR00MKUWw3Tq6Uce7jpJu40NXfOj-96GsvX28MF-P0P9d-iP8E_c2-Awx_fTzLcRuKJrlYcuTvKkYYYSoUlQPZH4PfuKQiw</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Eyuboglu, Atilla Adnan</creator><creator>Arpaci, Enver</creator><creator>Albayati, Abbas</creator><creator>Uysal, Ahmet Cagri</creator><creator>Terzi, Aysen</creator><creator>Bozalioglu, Sema</creator><creator>Turnaoglu, Hale</creator><creator>Balcik, Cenk</creator><creator>Ozkan, Burak</creator><creator>Ertas, Nilgun Markal</creator><general>Lippincott Williams & Wilkins</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200901</creationdate><title>The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease</title><author>Eyuboglu, Atilla Adnan ; Arpaci, Enver ; Albayati, Abbas ; Uysal, Ahmet Cagri ; Terzi, Aysen ; Bozalioglu, Sema ; Turnaoglu, Hale ; Balcik, Cenk ; Ozkan, Burak ; Ertas, Nilgun Markal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3523-fe9f75a128f95f4248d0196c46f704154d859b26259c231462f323a0431d23363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adipose Tissue</topic><topic>Animals</topic><topic>Platelet-Rich Plasma</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Renal Insufficiency, Chronic - therapy</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eyuboglu, Atilla Adnan</creatorcontrib><creatorcontrib>Arpaci, Enver</creatorcontrib><creatorcontrib>Albayati, Abbas</creatorcontrib><creatorcontrib>Uysal, Ahmet Cagri</creatorcontrib><creatorcontrib>Terzi, Aysen</creatorcontrib><creatorcontrib>Bozalioglu, Sema</creatorcontrib><creatorcontrib>Turnaoglu, Hale</creatorcontrib><creatorcontrib>Balcik, Cenk</creatorcontrib><creatorcontrib>Ozkan, Burak</creatorcontrib><creatorcontrib>Ertas, Nilgun Markal</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of plastic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eyuboglu, Atilla Adnan</au><au>Arpaci, Enver</au><au>Albayati, Abbas</au><au>Uysal, Ahmet Cagri</au><au>Terzi, Aysen</au><au>Bozalioglu, Sema</au><au>Turnaoglu, Hale</au><au>Balcik, Cenk</au><au>Ozkan, Burak</au><au>Ertas, Nilgun Markal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease</atitle><jtitle>Annals of plastic surgery</jtitle><addtitle>Ann Plast Surg</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>85</volume><issue>3</issue><spage>316</spage><epage>323</epage><pages>316-323</pages><issn>0148-7043</issn><eissn>1536-3708</eissn><abstract>Chronic kidney disease (CKD) impairs osteoblast/osteoclast balance and damages bone structure with diminished mineralization and results in bone restoration disorders. In this study, we investigate the effects of adipose-derived stromal vascular fraction and platelet-rich plasma (PRP) on bone healing model in rats with CKD.
Sprague-Dawley rats were separated into 4 groups. All groups except group I (healthy control) had CKD surgery using 5/6 nephrectomy model. All groups had intramedullary pin fixation after receiving bone fracture using drilling tools. Group II rats were used as control group for CKD. Group III rats received PRP treatment on fracture site. Group IV rats received PRP and stromal vascular fraction treatment on fracture site.Weight loss and blood samples were followed at the time of kidney surgery, third, sixth, and 12th weeks. Bone healing and callus formations were compared, biomechanically, radiologically, histopathologically, and immunohistochemically. Osteoblastic transformation of stem cells was assessed with DiI staining.
Negative effects of CKD on bone healing were reduced by increasing mechanical, histological, radiological, and biochemical properties of the bone with stromal vascular fraction and PRP treatments. Although thickness of callus tissue delayed bone healing process, it also enhanced biomechanical features and bone tissue organization.
Platelet-rich plasma and adipose-derived stromal vascular fraction treatments were effective for bone healing in animal model, which can be promising for clinical trials.</abstract><cop>United States</cop><pub>Lippincott Williams & Wilkins</pub><pmid>32784349</pmid><doi>10.1097/SAP.0000000000002396</doi><tpages>8</tpages></addata></record> |
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| subjects | Adipose Tissue Animals Platelet-Rich Plasma Rats Rats, Sprague-Dawley Renal Insufficiency, Chronic - therapy Wound Healing |
| title | The Effects of Adipose Derived Stromal Vascular Fraction and Platelet-Rich Plasma on Bone Healing of a Rat Model With Chronic Kidney Disease |
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