Erlotinib-Loaded Poly(ε-Caprolactone) Nanocapsules Improve In Vitro Cytotoxicity and Anticlonogenic Effects on Human A549 Lung Cancer Cells
Lung cancer is the most frequent type of cancer and the leading cause of cancer-related mortality worldwide. This study aimed to develop erlotinib (ELB)-loaded poly(ε-caprolactone) nanocapsules (NC ELB ) and evaluated their in vitro cytotoxicity in A549 cells. The formulation was characterized in re...
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| Veröffentlicht in: | AAPS PharmSciTech 2020-08, Vol.21 (6), p.229-229, Article 229 |
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| creator | Bruinsmann, Franciele Aline Buss, Julieti Huch Souto, Gabriele Dadalt Schultze, Eduarda de Cristo Soares Alves, Aline Seixas, Fabiana Kömmling Collares, Tiago Veiras Pohlmann, Adriana Raffin Guterres, Silvia Stanisçuaski |
| description | Lung cancer is the most frequent type of cancer and the leading cause of cancer-related mortality worldwide. This study aimed to develop erlotinib (ELB)-loaded poly(ε-caprolactone) nanocapsules (NC
ELB
) and evaluated their
in vitro
cytotoxicity in A549 cells. The formulation was characterized in relation to hydrodynamic diameter (171 nm), polydispersity index (0.076), zeta potential (− 8 mV), drug content (0.5 mg
.
mL
−1
), encapsulation efficiency (99%), and pH (6.0). NC
ELB
presented higher cytotoxicity than ELB in solution against A549 cells in the MTT and LIVE/DEAD cell viability assays after 24 h of treatment. The main mechanism of cytotoxicity of NC
ELB
was the induction of apoptosis in A549 cells. Further, a significant decrease in A549 colony formation was verified after NC
ELB
treatment in comparison with the unencapsulated drug treatment. The reduction in clonogenic capacity is very relevant as it can reduce the risk of tumor recurrence and metastasis. In conclusion, erlotinib-loaded PCL nanocapsules are promising nanoparticles carriers to increase the efficacy of ELB in lung cancer treatment. |
| doi_str_mv | 10.1208/s12249-020-01723-y |
| format | Article |
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ELB
) and evaluated their
in vitro
cytotoxicity in A549 cells. The formulation was characterized in relation to hydrodynamic diameter (171 nm), polydispersity index (0.076), zeta potential (− 8 mV), drug content (0.5 mg
.
mL
−1
), encapsulation efficiency (99%), and pH (6.0). NC
ELB
presented higher cytotoxicity than ELB in solution against A549 cells in the MTT and LIVE/DEAD cell viability assays after 24 h of treatment. The main mechanism of cytotoxicity of NC
ELB
was the induction of apoptosis in A549 cells. Further, a significant decrease in A549 colony formation was verified after NC
ELB
treatment in comparison with the unencapsulated drug treatment. The reduction in clonogenic capacity is very relevant as it can reduce the risk of tumor recurrence and metastasis. In conclusion, erlotinib-loaded PCL nanocapsules are promising nanoparticles carriers to increase the efficacy of ELB in lung cancer treatment.</description><identifier>ISSN: 1530-9932</identifier><identifier>EISSN: 1530-9932</identifier><identifier>DOI: 10.1208/s12249-020-01723-y</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Pharmacology/Toxicology ; Pharmacy ; Research Article</subject><ispartof>AAPS PharmSciTech, 2020-08, Vol.21 (6), p.229-229, Article 229</ispartof><rights>American Association of Pharmaceutical Scientists 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c239y-3ac31fefd20390b170b1d76df6b527eaebf321379333ffe7d184d541536978d43</citedby><cites>FETCH-LOGICAL-c239y-3ac31fefd20390b170b1d76df6b527eaebf321379333ffe7d184d541536978d43</cites><orcidid>0000-0002-8833-7542</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1208/s12249-020-01723-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1208/s12249-020-01723-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Bruinsmann, Franciele Aline</creatorcontrib><creatorcontrib>Buss, Julieti Huch</creatorcontrib><creatorcontrib>Souto, Gabriele Dadalt</creatorcontrib><creatorcontrib>Schultze, Eduarda</creatorcontrib><creatorcontrib>de Cristo Soares Alves, Aline</creatorcontrib><creatorcontrib>Seixas, Fabiana Kömmling</creatorcontrib><creatorcontrib>Collares, Tiago Veiras</creatorcontrib><creatorcontrib>Pohlmann, Adriana Raffin</creatorcontrib><creatorcontrib>Guterres, Silvia Stanisçuaski</creatorcontrib><title>Erlotinib-Loaded Poly(ε-Caprolactone) Nanocapsules Improve In Vitro Cytotoxicity and Anticlonogenic Effects on Human A549 Lung Cancer Cells</title><title>AAPS PharmSciTech</title><addtitle>AAPS PharmSciTech</addtitle><description>Lung cancer is the most frequent type of cancer and the leading cause of cancer-related mortality worldwide. This study aimed to develop erlotinib (ELB)-loaded poly(ε-caprolactone) nanocapsules (NC
ELB
) and evaluated their
in vitro
cytotoxicity in A549 cells. The formulation was characterized in relation to hydrodynamic diameter (171 nm), polydispersity index (0.076), zeta potential (− 8 mV), drug content (0.5 mg
.
mL
−1
), encapsulation efficiency (99%), and pH (6.0). NC
ELB
presented higher cytotoxicity than ELB in solution against A549 cells in the MTT and LIVE/DEAD cell viability assays after 24 h of treatment. The main mechanism of cytotoxicity of NC
ELB
was the induction of apoptosis in A549 cells. Further, a significant decrease in A549 colony formation was verified after NC
ELB
treatment in comparison with the unencapsulated drug treatment. The reduction in clonogenic capacity is very relevant as it can reduce the risk of tumor recurrence and metastasis. In conclusion, erlotinib-loaded PCL nanocapsules are promising nanoparticles carriers to increase the efficacy of ELB in lung cancer treatment.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Research Article</subject><issn>1530-9932</issn><issn>1530-9932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kDtOAzEQhlcIJJ4XoHIJhcH27CMuo1WASBFQAK3l2N7IaGMH24vYO3AdrsGZMISCimI0I83_z-MrilNKLigjk8tIGSs5JoxgQhsGeNwpDmgFBHMObPdPvV8cxvhMCAPK4aB4n4XeJ-vsEi-81Eaje9-PZ58fuJWb4HupknfmHN1K55XcxKE3Ec3XufVq0NyhJ5uCR-2YfPJvVtk0Iuk0mrpkVe-dXxlnFZp1nVEpIu_QzbCWDk2rkqPF4FaolU6ZgFrT9_G42OtkH83Jbz4qHq9mD-0NXtxdz9vpAisGfMQgFdDOdJoR4GRJmxy6qXVXLyvWGGmWHTAKDQeAvLjRdFLqqswIat5MdAlHxdl2bn7jZTAxibWNKl8gnfFDFKwENqlqVldZyrZSFXyMwXRiE-xahlFQIr7Riy16kdGLH_RizCbYmmIWu5UJ4tkPweWX_nN9AUSaiS8</recordid><startdate>20200810</startdate><enddate>20200810</enddate><creator>Bruinsmann, Franciele Aline</creator><creator>Buss, Julieti Huch</creator><creator>Souto, Gabriele Dadalt</creator><creator>Schultze, Eduarda</creator><creator>de Cristo Soares Alves, Aline</creator><creator>Seixas, Fabiana Kömmling</creator><creator>Collares, Tiago Veiras</creator><creator>Pohlmann, Adriana Raffin</creator><creator>Guterres, Silvia Stanisçuaski</creator><general>Springer International Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8833-7542</orcidid></search><sort><creationdate>20200810</creationdate><title>Erlotinib-Loaded Poly(ε-Caprolactone) Nanocapsules Improve In Vitro Cytotoxicity and Anticlonogenic Effects on Human A549 Lung Cancer Cells</title><author>Bruinsmann, Franciele Aline ; Buss, Julieti Huch ; Souto, Gabriele Dadalt ; Schultze, Eduarda ; de Cristo Soares Alves, Aline ; Seixas, Fabiana Kömmling ; Collares, Tiago Veiras ; Pohlmann, Adriana Raffin ; Guterres, Silvia Stanisçuaski</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c239y-3ac31fefd20390b170b1d76df6b527eaebf321379333ffe7d184d541536978d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Research Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bruinsmann, Franciele Aline</creatorcontrib><creatorcontrib>Buss, Julieti Huch</creatorcontrib><creatorcontrib>Souto, Gabriele Dadalt</creatorcontrib><creatorcontrib>Schultze, Eduarda</creatorcontrib><creatorcontrib>de Cristo Soares Alves, Aline</creatorcontrib><creatorcontrib>Seixas, Fabiana Kömmling</creatorcontrib><creatorcontrib>Collares, Tiago Veiras</creatorcontrib><creatorcontrib>Pohlmann, Adriana Raffin</creatorcontrib><creatorcontrib>Guterres, Silvia Stanisçuaski</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>AAPS PharmSciTech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bruinsmann, Franciele Aline</au><au>Buss, Julieti Huch</au><au>Souto, Gabriele Dadalt</au><au>Schultze, Eduarda</au><au>de Cristo Soares Alves, Aline</au><au>Seixas, Fabiana Kömmling</au><au>Collares, Tiago Veiras</au><au>Pohlmann, Adriana Raffin</au><au>Guterres, Silvia Stanisçuaski</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Erlotinib-Loaded Poly(ε-Caprolactone) Nanocapsules Improve In Vitro Cytotoxicity and Anticlonogenic Effects on Human A549 Lung Cancer Cells</atitle><jtitle>AAPS PharmSciTech</jtitle><stitle>AAPS PharmSciTech</stitle><date>2020-08-10</date><risdate>2020</risdate><volume>21</volume><issue>6</issue><spage>229</spage><epage>229</epage><pages>229-229</pages><artnum>229</artnum><issn>1530-9932</issn><eissn>1530-9932</eissn><abstract>Lung cancer is the most frequent type of cancer and the leading cause of cancer-related mortality worldwide. This study aimed to develop erlotinib (ELB)-loaded poly(ε-caprolactone) nanocapsules (NC
ELB
) and evaluated their
in vitro
cytotoxicity in A549 cells. The formulation was characterized in relation to hydrodynamic diameter (171 nm), polydispersity index (0.076), zeta potential (− 8 mV), drug content (0.5 mg
.
mL
−1
), encapsulation efficiency (99%), and pH (6.0). NC
ELB
presented higher cytotoxicity than ELB in solution against A549 cells in the MTT and LIVE/DEAD cell viability assays after 24 h of treatment. The main mechanism of cytotoxicity of NC
ELB
was the induction of apoptosis in A549 cells. Further, a significant decrease in A549 colony formation was verified after NC
ELB
treatment in comparison with the unencapsulated drug treatment. The reduction in clonogenic capacity is very relevant as it can reduce the risk of tumor recurrence and metastasis. In conclusion, erlotinib-loaded PCL nanocapsules are promising nanoparticles carriers to increase the efficacy of ELB in lung cancer treatment.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1208/s12249-020-01723-y</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-8833-7542</orcidid></addata></record> |
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| title | Erlotinib-Loaded Poly(ε-Caprolactone) Nanocapsules Improve In Vitro Cytotoxicity and Anticlonogenic Effects on Human A549 Lung Cancer Cells |
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