Influence of microfabrication on digital PCR performance in bead-based microwell array assays

Digital PCR (dPCR) is a highly sensitive analytical technique used to quantify DNA targets. Detection sensitivity can be further enhanced by capturing target sequences onto beads for preconcentration and sample cleanup prior to analysis in microfluidic microwell arrays. However, robust digital analy...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2020-10, Vol.412 (25), p.6917-6926
Hauptverfasser:	McCarthy Riley, Bailey F., Ward, Cassandra L., Linz, Thomas H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Arrays Assaying Beads Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Debris Detritus DNA - analysis Fabrication Food Science Heterogeneity Humans Laboratory Medicine Microfluidic Analytical Techniques - methods Microfluidics Monitoring/Environmental Analysis Nucleotide sequence Photoresists Polymerase chain reaction Polymerase Chain Reaction - methods Research Paper Robustness Scanning electron microscopy Sensitivity enhancement Silicon wafers Spatial heterogeneity Target detection Wells
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