Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus
Abstract Objectives Two labdane diterpenoids, leojapone B and heteronone B, were isolated from Leonurus japonicus Houtt., and their biological activity were evaluated in this study. Methods Human and mouse cancer cells, human peripheral blood mononuclear cells (PBMCs) and mouse macrophages (RAW264.7...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2020-11, Vol.72 (11), p.1657-1665 |
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| container_title | Journal of pharmacy and pharmacology |
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| creator | Yue, Grace Gar-Lee Liang, Xin-Xin Li, Xiao-Li Lee, Julia Kin-Ming Gao, Si Kwok, Hin-Fai Lau, Clara Bik-San Xiao, Wei-Lie |
| description | Abstract
Objectives
Two labdane diterpenoids, leojapone B and heteronone B, were isolated from Leonurus japonicus Houtt., and their biological activity were evaluated in this study.
Methods
Human and mouse cancer cells, human peripheral blood mononuclear cells (PBMCs) and mouse macrophages (RAW264.7 cells) were used to evaluate the activity of leojapone B and heteronone B, while the in vivo effects of leojapone B were further examined in Lewis Lung Cancer tumour-bearing mice.
Key findings
In vitro studies showed that leojapone B selectively inhibited the proliferation of lung cancer cells, and both leojapone B and heteronone B inhibited the production of pro-inflammatory cytokines in activated PBMCs. In tumour-bearing mice model, lung tumours were reduced in size in mice treated with intraperitoneal injections of leojapone B at 20 and 30 mg/kg for 14 days. The population ratio of CD4+/CD8+ T cells in mouse spleens was found to be increased, while regulatory T cells were decreased after leojapone B treatment.
Conclusions
The inhibitory effects of leojapone B in mouse lung tumours were demonstrated for the first time in this study. The immunomodulatory activity of heteronone B were also demonstrated. Our findings indicated that both leojapone B and heteronone B may act as active components in L. japonicus. |
| doi_str_mv | 10.1111/jphp.13348 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2430979326</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/jphp.13348</oup_id><sourcerecordid>2448516602</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2798-f7962a3dad42b5163e0b31ddbf6537f9f7de527b7f5177e3f1bee69e7c87adea3</originalsourceid><addsrcrecordid>eNp9kE9LxDAQxYMouK5e_AQFEUTomj9t0h5lUXdlwT2o15I2E8zSNjVplP32Zl29eHBgmMvvzbx5CJ0TPCOxbjbD2zAjjGXFAZpQnNFUkLw4RBOMKU1ZLtgxOvF-gzEWnPMJel12XehtZ1Vo5WjdNpG9ij2aMXQ2uKQ2Vjaj-YCklbWSPSTKjOAG6K1RPtHOdskKbB9c8MlGDrY3TfCn6EjL1sPZz5yil_u75_kiXT09LOe3q7ShoixSLUpOJVNSZbTOCWeAa0aUqjXPmdClFgpyKmqhcyIEME1qAF6CaAohFUg2RVf7vYOz7wH8WHXGN9C20agNvqIZw6UoGeURvfiDbuJ_fXQXqayI1zmmkbreU42z3jvQ1eBMJ922IrjaRVztIq6-I44w2cOfpoXtP2T1uF6sfzWXe40Nw3-7vwB1v4yK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2448516602</pqid></control><display><type>article</type><title>Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus</title><source>Wiley Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Yue, Grace Gar-Lee ; Liang, Xin-Xin ; Li, Xiao-Li ; Lee, Julia Kin-Ming ; Gao, Si ; Kwok, Hin-Fai ; Lau, Clara Bik-San ; Xiao, Wei-Lie</creator><creatorcontrib>Yue, Grace Gar-Lee ; Liang, Xin-Xin ; Li, Xiao-Li ; Lee, Julia Kin-Ming ; Gao, Si ; Kwok, Hin-Fai ; Lau, Clara Bik-San ; Xiao, Wei-Lie</creatorcontrib><description>Abstract
Objectives
Two labdane diterpenoids, leojapone B and heteronone B, were isolated from Leonurus japonicus Houtt., and their biological activity were evaluated in this study.
Methods
Human and mouse cancer cells, human peripheral blood mononuclear cells (PBMCs) and mouse macrophages (RAW264.7 cells) were used to evaluate the activity of leojapone B and heteronone B, while the in vivo effects of leojapone B were further examined in Lewis Lung Cancer tumour-bearing mice.
Key findings
In vitro studies showed that leojapone B selectively inhibited the proliferation of lung cancer cells, and both leojapone B and heteronone B inhibited the production of pro-inflammatory cytokines in activated PBMCs. In tumour-bearing mice model, lung tumours were reduced in size in mice treated with intraperitoneal injections of leojapone B at 20 and 30 mg/kg for 14 days. The population ratio of CD4+/CD8+ T cells in mouse spleens was found to be increased, while regulatory T cells were decreased after leojapone B treatment.
Conclusions
The inhibitory effects of leojapone B in mouse lung tumours were demonstrated for the first time in this study. The immunomodulatory activity of heteronone B were also demonstrated. Our findings indicated that both leojapone B and heteronone B may act as active components in L. japonicus.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.13348</identifier><language>eng</language><publisher>Bognor Regis: Oxford University Press</publisher><subject>Biological activity ; CD4 antigen ; CD8 antigen ; Cell proliferation ; Cytokines ; cytotoxicity ; Diterpenes ; heteronone ; Immunomodulation ; Immunoregulation ; Inflammation ; labdane diterpenoids ; leojapone ; Leonurus japonicus ; Leukocytes (mononuclear) ; lewis lung cancer ; Lung cancer ; Lymphocytes T ; Macrophages ; Peripheral blood mononuclear cells ; Tumors</subject><ispartof>Journal of pharmacy and pharmacology, 2020-11, Vol.72 (11), p.1657-1665</ispartof><rights>2020 Royal Pharmaceutical Society 2020</rights><rights>2020 Royal Pharmaceutical Society</rights><rights>Copyright © 2020 Royal Pharmaceutical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2798-f7962a3dad42b5163e0b31ddbf6537f9f7de527b7f5177e3f1bee69e7c87adea3</citedby><cites>FETCH-LOGICAL-c2798-f7962a3dad42b5163e0b31ddbf6537f9f7de527b7f5177e3f1bee69e7c87adea3</cites><orcidid>0000-0001-6826-1993 ; 0000-0002-7409-1270 ; 0000-0002-1726-3566</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.13348$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.13348$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Yue, Grace Gar-Lee</creatorcontrib><creatorcontrib>Liang, Xin-Xin</creatorcontrib><creatorcontrib>Li, Xiao-Li</creatorcontrib><creatorcontrib>Lee, Julia Kin-Ming</creatorcontrib><creatorcontrib>Gao, Si</creatorcontrib><creatorcontrib>Kwok, Hin-Fai</creatorcontrib><creatorcontrib>Lau, Clara Bik-San</creatorcontrib><creatorcontrib>Xiao, Wei-Lie</creatorcontrib><title>Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus</title><title>Journal of pharmacy and pharmacology</title><description>Abstract
Objectives
Two labdane diterpenoids, leojapone B and heteronone B, were isolated from Leonurus japonicus Houtt., and their biological activity were evaluated in this study.
Methods
Human and mouse cancer cells, human peripheral blood mononuclear cells (PBMCs) and mouse macrophages (RAW264.7 cells) were used to evaluate the activity of leojapone B and heteronone B, while the in vivo effects of leojapone B were further examined in Lewis Lung Cancer tumour-bearing mice.
Key findings
In vitro studies showed that leojapone B selectively inhibited the proliferation of lung cancer cells, and both leojapone B and heteronone B inhibited the production of pro-inflammatory cytokines in activated PBMCs. In tumour-bearing mice model, lung tumours were reduced in size in mice treated with intraperitoneal injections of leojapone B at 20 and 30 mg/kg for 14 days. The population ratio of CD4+/CD8+ T cells in mouse spleens was found to be increased, while regulatory T cells were decreased after leojapone B treatment.
Conclusions
The inhibitory effects of leojapone B in mouse lung tumours were demonstrated for the first time in this study. The immunomodulatory activity of heteronone B were also demonstrated. Our findings indicated that both leojapone B and heteronone B may act as active components in L. japonicus.</description><subject>Biological activity</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell proliferation</subject><subject>Cytokines</subject><subject>cytotoxicity</subject><subject>Diterpenes</subject><subject>heteronone</subject><subject>Immunomodulation</subject><subject>Immunoregulation</subject><subject>Inflammation</subject><subject>labdane diterpenoids</subject><subject>leojapone</subject><subject>Leonurus japonicus</subject><subject>Leukocytes (mononuclear)</subject><subject>lewis lung cancer</subject><subject>Lung cancer</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Peripheral blood mononuclear cells</subject><subject>Tumors</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYMouK5e_AQFEUTomj9t0h5lUXdlwT2o15I2E8zSNjVplP32Zl29eHBgmMvvzbx5CJ0TPCOxbjbD2zAjjGXFAZpQnNFUkLw4RBOMKU1ZLtgxOvF-gzEWnPMJel12XehtZ1Vo5WjdNpG9ij2aMXQ2uKQ2Vjaj-YCklbWSPSTKjOAG6K1RPtHOdskKbB9c8MlGDrY3TfCn6EjL1sPZz5yil_u75_kiXT09LOe3q7ShoixSLUpOJVNSZbTOCWeAa0aUqjXPmdClFgpyKmqhcyIEME1qAF6CaAohFUg2RVf7vYOz7wH8WHXGN9C20agNvqIZw6UoGeURvfiDbuJ_fXQXqayI1zmmkbreU42z3jvQ1eBMJ922IrjaRVztIq6-I44w2cOfpoXtP2T1uF6sfzWXe40Nw3-7vwB1v4yK</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Yue, Grace Gar-Lee</creator><creator>Liang, Xin-Xin</creator><creator>Li, Xiao-Li</creator><creator>Lee, Julia Kin-Ming</creator><creator>Gao, Si</creator><creator>Kwok, Hin-Fai</creator><creator>Lau, Clara Bik-San</creator><creator>Xiao, Wei-Lie</creator><general>Oxford University Press</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6826-1993</orcidid><orcidid>https://orcid.org/0000-0002-7409-1270</orcidid><orcidid>https://orcid.org/0000-0002-1726-3566</orcidid></search><sort><creationdate>202011</creationdate><title>Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus</title><author>Yue, Grace Gar-Lee ; Liang, Xin-Xin ; Li, Xiao-Li ; Lee, Julia Kin-Ming ; Gao, Si ; Kwok, Hin-Fai ; Lau, Clara Bik-San ; Xiao, Wei-Lie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2798-f7962a3dad42b5163e0b31ddbf6537f9f7de527b7f5177e3f1bee69e7c87adea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biological activity</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Cell proliferation</topic><topic>Cytokines</topic><topic>cytotoxicity</topic><topic>Diterpenes</topic><topic>heteronone</topic><topic>Immunomodulation</topic><topic>Immunoregulation</topic><topic>Inflammation</topic><topic>labdane diterpenoids</topic><topic>leojapone</topic><topic>Leonurus japonicus</topic><topic>Leukocytes (mononuclear)</topic><topic>lewis lung cancer</topic><topic>Lung cancer</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Peripheral blood mononuclear cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yue, Grace Gar-Lee</creatorcontrib><creatorcontrib>Liang, Xin-Xin</creatorcontrib><creatorcontrib>Li, Xiao-Li</creatorcontrib><creatorcontrib>Lee, Julia Kin-Ming</creatorcontrib><creatorcontrib>Gao, Si</creatorcontrib><creatorcontrib>Kwok, Hin-Fai</creatorcontrib><creatorcontrib>Lau, Clara Bik-San</creatorcontrib><creatorcontrib>Xiao, Wei-Lie</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yue, Grace Gar-Lee</au><au>Liang, Xin-Xin</au><au>Li, Xiao-Li</au><au>Lee, Julia Kin-Ming</au><au>Gao, Si</au><au>Kwok, Hin-Fai</au><au>Lau, Clara Bik-San</au><au>Xiao, Wei-Lie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><date>2020-11</date><risdate>2020</risdate><volume>72</volume><issue>11</issue><spage>1657</spage><epage>1665</epage><pages>1657-1665</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Abstract
Objectives
Two labdane diterpenoids, leojapone B and heteronone B, were isolated from Leonurus japonicus Houtt., and their biological activity were evaluated in this study.
Methods
Human and mouse cancer cells, human peripheral blood mononuclear cells (PBMCs) and mouse macrophages (RAW264.7 cells) were used to evaluate the activity of leojapone B and heteronone B, while the in vivo effects of leojapone B were further examined in Lewis Lung Cancer tumour-bearing mice.
Key findings
In vitro studies showed that leojapone B selectively inhibited the proliferation of lung cancer cells, and both leojapone B and heteronone B inhibited the production of pro-inflammatory cytokines in activated PBMCs. In tumour-bearing mice model, lung tumours were reduced in size in mice treated with intraperitoneal injections of leojapone B at 20 and 30 mg/kg for 14 days. The population ratio of CD4+/CD8+ T cells in mouse spleens was found to be increased, while regulatory T cells were decreased after leojapone B treatment.
Conclusions
The inhibitory effects of leojapone B in mouse lung tumours were demonstrated for the first time in this study. The immunomodulatory activity of heteronone B were also demonstrated. Our findings indicated that both leojapone B and heteronone B may act as active components in L. japonicus.</abstract><cop>Bognor Regis</cop><pub>Oxford University Press</pub><doi>10.1111/jphp.13348</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6826-1993</orcidid><orcidid>https://orcid.org/0000-0002-7409-1270</orcidid><orcidid>https://orcid.org/0000-0002-1726-3566</orcidid></addata></record> |
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| source | Wiley Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological activity CD4 antigen CD8 antigen Cell proliferation Cytokines cytotoxicity Diterpenes heteronone Immunomodulation Immunoregulation Inflammation labdane diterpenoids leojapone Leonurus japonicus Leukocytes (mononuclear) lewis lung cancer Lung cancer Lymphocytes T Macrophages Peripheral blood mononuclear cells Tumors |
| title | Immunomodulatory and antitumour bioactive labdane diterpenoids from Leonurus japonicus |
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