Effect of macrophages on biological function of ovarian cancer cells in tumor microenvironment in vitro
Objective To investigate the influence of two types of tumor-associated macrophages (TAMs) on the biological function of human ovarian cell lines in vitro. Methods (1) M2 macrophage release was induced by IL-4, and M1 macrophage release by phorbol myristate acetate (PMA) in vitro. Flow cytometry was...
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| Veröffentlicht in: | Archives of gynecology and obstetrics 2020-10, Vol.302 (4), p.1009-1017 |
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| creator | Yi, Jiang Lin, Yuan Yicong, Wan Chengyan, Luo Shulin, Zhou Wenjun, Cheng |
| description | Objective
To investigate the influence of two types of tumor-associated macrophages (TAMs) on the biological function of human ovarian cell lines in vitro.
Methods
(1) M2 macrophage release was induced by IL-4, and M1 macrophage release by phorbol myristate acetate (PMA) in vitro. Flow cytometry was used to distinguish these two types; (2) transwell culture system was used to establish a non-contact co-culture model of macrophage and ovarian cancer cells (SKOV3, HEY, HO8910 and A2780) in vitro. The microenvironment of ovarian cancer was simulated in vitro. (3) The proliferation, apoptosis, migration and invasion of ovarian cancer cells SKOV3, HEY, HO8910 and A2780 were analyzed after co-culture. Their proliferation was detected by CCK8 method, apoptosis by flow cytometry, Annexin V-FITC/PI double staining, invasion by Transwell assay, and migration by wound healing test.
Results
(1) IL-4-induced macrophages (M2) overexpressed CD163, and PMA-induced macrophages (M1) overexpressed HLA-DR. After co-culturing primary macrophages with ovarian cancer cells (SKOV3, HEY, HO8910, A2780), macrophage CD163 was highly expressed. (2) Proliferation and apoptosis of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the proliferation of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
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| doi_str_mv | 10.1007/s00404-020-05719-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2430370509</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2430370509</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-e6ef3c1ec3f8dce7bbfee7d707c68c0ad43da34c531a28c95ec6d2924539502a3</originalsourceid><addsrcrecordid>eNp9kU2LFDEQhoMo7jj6BzxIwIuX1spXJ32UZf2ABS96Dpl0ZczSnYxJ94D_ftPOquDBU4Wqp96q1EvISwZvGYB-VwEkyA44dKA0GzrziOyYFLwDzdhjsoNhe0Ovr8izWu8AGDemf0quBNfSgJI7crwJAf1Cc6Cz8yWfvrsjVpoTPcQ85WP0bqJhTX6JLdeofHYlukS9Sx4L9ThNlcZEl3XOhc6xaWA6x5LTjGnZKue4lPycPAluqvjiIe7Jtw83X68_dbdfPn6-fn_beaHV0mGPQXiGXgQzetSHQ0DUowbte-PBjVKMTkivBHPc-EGh70c-cKnEoIA7sSdvLrqnkn-sWBc7x7ot6RLmtVouBQgNqp1mT17_g97ltaS23UYNWhnNRaP4hWofq7VgsKcSZ1d-WgZ2s8FebLDNBvvLBmta06sH6fUw4_in5ffdGyAuQG2ldMTyd_Z_ZO8By9KUDQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2439758723</pqid></control><display><type>article</type><title>Effect of macrophages on biological function of ovarian cancer cells in tumor microenvironment in vitro</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Yi, Jiang ; Lin, Yuan ; Yicong, Wan ; Chengyan, Luo ; Shulin, Zhou ; Wenjun, Cheng</creator><creatorcontrib>Yi, Jiang ; Lin, Yuan ; Yicong, Wan ; Chengyan, Luo ; Shulin, Zhou ; Wenjun, Cheng</creatorcontrib><description>Objective
To investigate the influence of two types of tumor-associated macrophages (TAMs) on the biological function of human ovarian cell lines in vitro.
Methods
(1) M2 macrophage release was induced by IL-4, and M1 macrophage release by phorbol myristate acetate (PMA) in vitro. Flow cytometry was used to distinguish these two types; (2) transwell culture system was used to establish a non-contact co-culture model of macrophage and ovarian cancer cells (SKOV3, HEY, HO8910 and A2780) in vitro. The microenvironment of ovarian cancer was simulated in vitro. (3) The proliferation, apoptosis, migration and invasion of ovarian cancer cells SKOV3, HEY, HO8910 and A2780 were analyzed after co-culture. Their proliferation was detected by CCK8 method, apoptosis by flow cytometry, Annexin V-FITC/PI double staining, invasion by Transwell assay, and migration by wound healing test.
Results
(1) IL-4-induced macrophages (M2) overexpressed CD163, and PMA-induced macrophages (M1) overexpressed HLA-DR. After co-culturing primary macrophages with ovarian cancer cells (SKOV3, HEY, HO8910, A2780), macrophage CD163 was highly expressed. (2) Proliferation and apoptosis of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the proliferation of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05); the apoptosis of ovarian cancer cells in M2 co-culture group decreased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05). (3) Migration and invasion of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the invasion of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05); the migration of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and the primary co-culture group (
p
< 0.05).
Conclusion
In the simulated in vitro tumor microenvironment, co-cultured ovarian cancer cells polarized macrophages to the M2 phenotype. Furthermore, M2 macrophages enhanced the proliferation, invasion and migration, and inhibited the apoptosis of ovarian cancer cells.</description><identifier>ISSN: 0932-0067</identifier><identifier>EISSN: 1432-0711</identifier><identifier>DOI: 10.1007/s00404-020-05719-8</identifier><identifier>PMID: 32748054</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Apoptosis ; Cell Line, Tumor ; Endocrinology ; Female ; Flow cytometry ; Gynecologic Oncology ; Gynecology ; Human Genetics ; Humans ; Macrophages - pathology ; Medicine ; Medicine & Public Health ; Obstetrics/Perinatology/Midwifery ; Ovarian cancer ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Tumor Microenvironment</subject><ispartof>Archives of gynecology and obstetrics, 2020-10, Vol.302 (4), p.1009-1017</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-e6ef3c1ec3f8dce7bbfee7d707c68c0ad43da34c531a28c95ec6d2924539502a3</citedby><cites>FETCH-LOGICAL-c375t-e6ef3c1ec3f8dce7bbfee7d707c68c0ad43da34c531a28c95ec6d2924539502a3</cites><orcidid>0000-0002-3872-4384</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00404-020-05719-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00404-020-05719-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32748054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, Jiang</creatorcontrib><creatorcontrib>Lin, Yuan</creatorcontrib><creatorcontrib>Yicong, Wan</creatorcontrib><creatorcontrib>Chengyan, Luo</creatorcontrib><creatorcontrib>Shulin, Zhou</creatorcontrib><creatorcontrib>Wenjun, Cheng</creatorcontrib><title>Effect of macrophages on biological function of ovarian cancer cells in tumor microenvironment in vitro</title><title>Archives of gynecology and obstetrics</title><addtitle>Arch Gynecol Obstet</addtitle><addtitle>Arch Gynecol Obstet</addtitle><description>Objective
To investigate the influence of two types of tumor-associated macrophages (TAMs) on the biological function of human ovarian cell lines in vitro.
Methods
(1) M2 macrophage release was induced by IL-4, and M1 macrophage release by phorbol myristate acetate (PMA) in vitro. Flow cytometry was used to distinguish these two types; (2) transwell culture system was used to establish a non-contact co-culture model of macrophage and ovarian cancer cells (SKOV3, HEY, HO8910 and A2780) in vitro. The microenvironment of ovarian cancer was simulated in vitro. (3) The proliferation, apoptosis, migration and invasion of ovarian cancer cells SKOV3, HEY, HO8910 and A2780 were analyzed after co-culture. Their proliferation was detected by CCK8 method, apoptosis by flow cytometry, Annexin V-FITC/PI double staining, invasion by Transwell assay, and migration by wound healing test.
Results
(1) IL-4-induced macrophages (M2) overexpressed CD163, and PMA-induced macrophages (M1) overexpressed HLA-DR. After co-culturing primary macrophages with ovarian cancer cells (SKOV3, HEY, HO8910, A2780), macrophage CD163 was highly expressed. (2) Proliferation and apoptosis of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the proliferation of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05); the apoptosis of ovarian cancer cells in M2 co-culture group decreased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05). (3) Migration and invasion of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the invasion of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05); the migration of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and the primary co-culture group (
p
< 0.05).
Conclusion
In the simulated in vitro tumor microenvironment, co-cultured ovarian cancer cells polarized macrophages to the M2 phenotype. Furthermore, M2 macrophages enhanced the proliferation, invasion and migration, and inhibited the apoptosis of ovarian cancer cells.</description><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Endocrinology</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Gynecologic Oncology</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Macrophages - pathology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Tumor Microenvironment</subject><issn>0932-0067</issn><issn>1432-0711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kU2LFDEQhoMo7jj6BzxIwIuX1spXJ32UZf2ABS96Dpl0ZczSnYxJ94D_ftPOquDBU4Wqp96q1EvISwZvGYB-VwEkyA44dKA0GzrziOyYFLwDzdhjsoNhe0Ovr8izWu8AGDemf0quBNfSgJI7crwJAf1Cc6Cz8yWfvrsjVpoTPcQ85WP0bqJhTX6JLdeofHYlukS9Sx4L9ThNlcZEl3XOhc6xaWA6x5LTjGnZKue4lPycPAluqvjiIe7Jtw83X68_dbdfPn6-fn_beaHV0mGPQXiGXgQzetSHQ0DUowbte-PBjVKMTkivBHPc-EGh70c-cKnEoIA7sSdvLrqnkn-sWBc7x7ot6RLmtVouBQgNqp1mT17_g97ltaS23UYNWhnNRaP4hWofq7VgsKcSZ1d-WgZ2s8FebLDNBvvLBmta06sH6fUw4_in5ffdGyAuQG2ldMTyd_Z_ZO8By9KUDQ</recordid><startdate>20201001</startdate><enddate>20201001</enddate><creator>Yi, Jiang</creator><creator>Lin, Yuan</creator><creator>Yicong, Wan</creator><creator>Chengyan, Luo</creator><creator>Shulin, Zhou</creator><creator>Wenjun, Cheng</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3872-4384</orcidid></search><sort><creationdate>20201001</creationdate><title>Effect of macrophages on biological function of ovarian cancer cells in tumor microenvironment in vitro</title><author>Yi, Jiang ; Lin, Yuan ; Yicong, Wan ; Chengyan, Luo ; Shulin, Zhou ; Wenjun, Cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-e6ef3c1ec3f8dce7bbfee7d707c68c0ad43da34c531a28c95ec6d2924539502a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Endocrinology</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Gynecologic Oncology</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Macrophages - pathology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Tumor Microenvironment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yi, Jiang</creatorcontrib><creatorcontrib>Lin, Yuan</creatorcontrib><creatorcontrib>Yicong, Wan</creatorcontrib><creatorcontrib>Chengyan, Luo</creatorcontrib><creatorcontrib>Shulin, Zhou</creatorcontrib><creatorcontrib>Wenjun, Cheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, Jiang</au><au>Lin, Yuan</au><au>Yicong, Wan</au><au>Chengyan, Luo</au><au>Shulin, Zhou</au><au>Wenjun, Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of macrophages on biological function of ovarian cancer cells in tumor microenvironment in vitro</atitle><jtitle>Archives of gynecology and obstetrics</jtitle><stitle>Arch Gynecol Obstet</stitle><addtitle>Arch Gynecol Obstet</addtitle><date>2020-10-01</date><risdate>2020</risdate><volume>302</volume><issue>4</issue><spage>1009</spage><epage>1017</epage><pages>1009-1017</pages><issn>0932-0067</issn><eissn>1432-0711</eissn><abstract>Objective
To investigate the influence of two types of tumor-associated macrophages (TAMs) on the biological function of human ovarian cell lines in vitro.
Methods
(1) M2 macrophage release was induced by IL-4, and M1 macrophage release by phorbol myristate acetate (PMA) in vitro. Flow cytometry was used to distinguish these two types; (2) transwell culture system was used to establish a non-contact co-culture model of macrophage and ovarian cancer cells (SKOV3, HEY, HO8910 and A2780) in vitro. The microenvironment of ovarian cancer was simulated in vitro. (3) The proliferation, apoptosis, migration and invasion of ovarian cancer cells SKOV3, HEY, HO8910 and A2780 were analyzed after co-culture. Their proliferation was detected by CCK8 method, apoptosis by flow cytometry, Annexin V-FITC/PI double staining, invasion by Transwell assay, and migration by wound healing test.
Results
(1) IL-4-induced macrophages (M2) overexpressed CD163, and PMA-induced macrophages (M1) overexpressed HLA-DR. After co-culturing primary macrophages with ovarian cancer cells (SKOV3, HEY, HO8910, A2780), macrophage CD163 was highly expressed. (2) Proliferation and apoptosis of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the proliferation of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05); the apoptosis of ovarian cancer cells in M2 co-culture group decreased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05). (3) Migration and invasion of ovarian cancer cells (SKOV3, HEY, HO8910, A2780): the invasion of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and primary co-culture group (
p
< 0.05); the migration of ovarian cancer cells in M2 co-culture group increased compared to that in M1 co-culture group and the primary co-culture group (
p
< 0.05).
Conclusion
In the simulated in vitro tumor microenvironment, co-cultured ovarian cancer cells polarized macrophages to the M2 phenotype. Furthermore, M2 macrophages enhanced the proliferation, invasion and migration, and inhibited the apoptosis of ovarian cancer cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32748054</pmid><doi>10.1007/s00404-020-05719-8</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-3872-4384</orcidid></addata></record> |
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| identifier | ISSN: 0932-0067 |
| ispartof | Archives of gynecology and obstetrics, 2020-10, Vol.302 (4), p.1009-1017 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Apoptosis Cell Line, Tumor Endocrinology Female Flow cytometry Gynecologic Oncology Gynecology Human Genetics Humans Macrophages - pathology Medicine Medicine & Public Health Obstetrics/Perinatology/Midwifery Ovarian cancer Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Tumor Microenvironment |
| title | Effect of macrophages on biological function of ovarian cancer cells in tumor microenvironment in vitro |
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